YGL085W_p / LCL3

Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL085W / LCL3

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:-3.06|P-value:6.47E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.44|P-value:1.38E-6 ID:SGTC_135|Compound:0933-0004|FD-Score:3.03|P-value:6.88E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.02|P-value:7.54E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.07|P-value:5.99E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.00|P-value:7.79E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:3.16|P-value:4.26E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.90|P-value:1.97E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:2.98|P-value:8.22E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.08|P-value:5.73E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:4.56|P-value:7.51E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:2.97|P-value:8.63E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.52|P-value:9.94E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.25|P-value:3.16E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.09|P-value:5.47E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.11|P-value:5.11E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-4.39|P-value:2.01E-6 ID:SGTC_1594|Compound:piperine|FD-Score:3.32|P-value:2.32E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.04|P-value:7.15E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-3.32|P-value:2.40E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.86|P-value:2.36E-5 ID:SGTC_1759|Compound:st043059|FD-Score:4.04|P-value:1.02E-5 ID:SGTC_1822|Compound:st052468|FD-Score:-3.41|P-value:1.71E-4 ID:SGTC_2112|Compound:5488546|FD-Score:5.52|P-value:2.92E-9 ID:SGTC_2208|Compound:7283366|FD-Score:3.13|P-value:4.67E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.64|P-value:6.00E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.58|P-value:7.93E-5 ID:SGTC_2446|Compound:5162541|FD-Score:-3.73|P-value:4.46E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.37|P-value:1.85E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.10|P-value:5.68E-4 ID:SGTC_2809|Compound:7964133|FD-Score:3.81|P-value:2.92E-5 ID:SGTC_2811|Compound:7967822|FD-Score:-3.22|P-value:3.60E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.51|P-value:1.12E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.34|P-value:2.28E-4 ID:SGTC_2859|Compound:9028643|FD-Score:3.51|P-value:1.08E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.30|P-value:2.50E-4 ID:SGTC_2994|Compound:9053520|FD-Score:3.23|P-value:3.31E-4 ID:SGTC_3049|Compound:9092223|FD-Score:4.68|P-value:4.03E-7 ID:SGTC_3099|Compound:9120367|FD-Score:-3.14|P-value:4.85E-4 ID:SGTC_3334|Compound:9142812|FD-Score:3.00|P-value:7.81E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.06|P-value:6.47E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.44|P-value:1.38E-6 ID:SGTC_135|Compound:0933-0004|FD-Score:3.03|P-value:6.88E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-3.02|P-value:7.54E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.07|P-value:5.99E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:3.00|P-value:7.79E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:3.16|P-value:4.26E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.90|P-value:1.97E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:2.98|P-value:8.22E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.08|P-value:5.73E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:4.56|P-value:7.51E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:2.97|P-value:8.63E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:3.52|P-value:9.94E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.25|P-value:3.16E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.09|P-value:5.47E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.11|P-value:5.11E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-4.39|P-value:2.01E-6 ID:SGTC_1594|Compound:piperine|FD-Score:3.32|P-value:2.32E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.04|P-value:7.15E-4 ID:SGTC_1642|Compound:st009582|FD-Score:-3.32|P-value:2.40E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.86|P-value:2.36E-5 ID:SGTC_1759|Compound:st043059|FD-Score:4.04|P-value:1.02E-5 ID:SGTC_1822|Compound:st052468|FD-Score:-3.41|P-value:1.71E-4 ID:SGTC_2112|Compound:5488546|FD-Score:5.52|P-value:2.92E-9 ID:SGTC_2208|Compound:7283366|FD-Score:3.13|P-value:4.67E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.64|P-value:6.00E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.58|P-value:7.93E-5 ID:SGTC_2446|Compound:5162541|FD-Score:-3.73|P-value:4.46E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.37|P-value:1.85E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.10|P-value:5.68E-4 ID:SGTC_2809|Compound:7964133|FD-Score:3.81|P-value:2.92E-5 ID:SGTC_2811|Compound:7967822|FD-Score:-3.22|P-value:3.60E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.51|P-value:1.12E-4 ID:SGTC_2857|Compound:9024691|FD-Score:-3.34|P-value:2.28E-4 ID:SGTC_2859|Compound:9028643|FD-Score:3.51|P-value:1.08E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.30|P-value:2.50E-4 ID:SGTC_2994|Compound:9053520|FD-Score:3.23|P-value:3.31E-4 ID:SGTC_3049|Compound:9092223|FD-Score:4.68|P-value:4.03E-7 ID:SGTC_3099|Compound:9120367|FD-Score:-3.14|P-value:4.85E-4 ID:SGTC_3334|Compound:9142812|FD-Score:3.00|P-value:7.81E-4

Top fitness defect scores for YGL085W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2112 5488546 5.52 200.0 µM 2.92E-9 TSC3-RPN4 5 1.70E-8
2 SGTC_3049 9092223 4.68 49.5 µM 4.03E-7 8 1.46E-6
3 SGTC_659 k018-0002 4.56 107.0 µM 7.51E-7 11 2.56E-6
4 SGTC_114 0364-0001 4.44 490.3 µM 1.38E-6 32 4.42E-6
5 SGTC_1759 st043059 4.04 70.0 µM 1.02E-5 amide catabolism 27 2.70E-5
6 SGTC_383 2324-0395 3.90 7.4 µM 1.97E-5 azole & statin 34 4.90E-5
7 SGTC_1728 st035614 3.86 33.8 µM 2.36E-5 56 5.78E-5
8 SGTC_2809 7964133 3.81 13.0 µM 2.92E-5 60S ribosome export 44 7.00E-5
9 SGTC_2444 5485484 3.64 36.4 µM 6.00E-5 42 1.34E-4
10 SGTC_2445 5742597 3.58 35.6 µM 7.93E-5 47 1.73E-4
11 SGTC_997 3996-0078 3.52 137.0 µM 9.94E-5 40 2.12E-4
12 SGTC_2859 9028643 3.51 45.5 µM 1.08E-4 27 2.28E-4
13 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 3.37 63.6 µM 1.85E-4 67 3.72E-4
14 SGTC_1594 piperine 3.32 70.1 µM 2.32E-4 34 4.57E-4
15 SGTC_2906 7926292 3.30 26.4 µM 2.50E-4 45 4.88E-4
16 SGTC_2994 9053520 3.23 71.4 µM 3.31E-4 RPP1 & pyrimidine depletion 42 6.29E-4
17 SGTC_356 1634-0012 3.16 32.5 µM 4.26E-4 55 7.93E-4
18 SGTC_2208 7283366 3.13 200.0 µM 4.67E-4 31 8.61E-4
19 SGTC_1575 cyproterone acetate 3.11 48.0 µM 5.11E-4 54 9.33E-4
20 SGTC_1430 4049-0248 3.09 69.1 µM 5.47E-4 52 9.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 8.65E-12 YJR119C JHD2 hom JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation
0.110 1.99E-10 YDL072C YET3 hom Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
0.108 4.21E-10 YOR245C DGA1 hom Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
0.107 6.21E-10 YLL023C POM33 hom Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
0.098 1.51E-8 YPL187W MF(ALPHA)1 hom Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor
0.088 3.90E-7 YGR172C YIP1 het Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
0.086 6.12E-7 YDR026C NSI1 hom RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
0.085 8.91E-7 YOR081C TGL5 hom Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate
0.079 4.45E-6 YHR049C-A_d YHR049C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.075 1.27E-5 YGR249W MGA1 hom Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
0.074 1.92E-5 YML028W TSA1 hom Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
0.073 2.65E-5 YBL107C MIC23 hom Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C)
0.070 4.63E-5 YER055C HIS1 hom ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
0.070 4.94E-5 YDR019C GCV1 hom T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
0.069 6.27E-5 YBL075C SSA3 hom ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication