YGL090W / LIF1

Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL090W / LIF1

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:4.57|P-value:3.13E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:-17.60|P-value:3.58E-68 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.37|P-value:7.66E-6 ID:SGTC_49|Compound:0717-0965|FD-Score:3.16|P-value:9.03E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-7.23|P-value:4.75E-13 ID:SGTC_125|Compound:0133-0007|FD-Score:3.80|P-value:8.77E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.53|P-value:2.39E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.34|P-value:4.96E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.25|P-value:6.62E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.94|P-value:4.86E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.93|P-value:5.12E-5 ID:SGTC_575|Compound:k072-0246|FD-Score:3.69|P-value:1.31E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.45|P-value:3.29E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.75|P-value:1.08E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:3.32|P-value:5.08E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-9.68|P-value:6.16E-22 ID:SGTC_867|Compound:0518-0118|FD-Score:3.34|P-value:4.79E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:5.12|P-value:2.10E-7 ID:SGTC_925|Compound:2064-1272|FD-Score:3.37|P-value:4.30E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.80|P-value:8.71E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.28|P-value:5.86E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.32|P-value:5.07E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:3.77|P-value:9.65E-5 ID:SGTC_1740|Compound:st038286|FD-Score:6.65|P-value:2.42E-11 ID:SGTC_1741|Compound:st038134|FD-Score:-4.63|P-value:2.41E-6 ID:SGTC_1744|Compound:st037937|FD-Score:5.05|P-value:3.05E-7 ID:SGTC_1773|Compound:st048542|FD-Score:4.16|P-value:1.97E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.60|P-value:1.90E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.25|P-value:6.67E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.91|P-value:5.62E-5 ID:SGTC_1794|Compound:st048918|FD-Score:5.13|P-value:1.94E-7 ID:SGTC_1796|Compound:st049173|FD-Score:3.67|P-value:1.42E-4 ID:SGTC_1986|Compound:st071812|FD-Score:3.30|P-value:5.52E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.31|P-value:5.48E-4 ID:SGTC_2033|Compound:5192550|FD-Score:4.28|P-value:1.16E-5 ID:SGTC_2088|Compound:5278280|FD-Score:3.20|P-value:7.69E-4 ID:SGTC_2198|Compound:6589944|FD-Score:3.44|P-value:3.32E-4 ID:SGTC_2300|Compound:7976697|FD-Score:3.42|P-value:3.56E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.43|P-value:3.49E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.13|P-value:9.92E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.40|P-value:3.88E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.90|P-value:5.88E-5 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.44|P-value:3.37E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.32|P-value:5.08E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:5.15|P-value:1.80E-7 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.56|P-value:3.30E-6 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.63|P-value:2.36E-6 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.85|P-value:7.14E-5 ID:SGTC_2792|Compound:7535235|FD-Score:-5.47|P-value:3.32E-8 ID:SGTC_2936|Compound:9038235|FD-Score:3.35|P-value:4.70E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.38|P-value:4.11E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.28|P-value:5.83E-4 ID:SGTC_3352|Compound:9151559|FD-Score:4.44|P-value:5.76E-6 ID:SGTC_39|Compound:0986-0283|FD-Score:4.57|P-value:3.13E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:-17.60|P-value:3.58E-68 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.37|P-value:7.66E-6 ID:SGTC_49|Compound:0717-0965|FD-Score:3.16|P-value:9.03E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-7.23|P-value:4.75E-13 ID:SGTC_125|Compound:0133-0007|FD-Score:3.80|P-value:8.77E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.53|P-value:2.39E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.34|P-value:4.96E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:3.25|P-value:6.62E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.94|P-value:4.86E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.93|P-value:5.12E-5 ID:SGTC_575|Compound:k072-0246|FD-Score:3.69|P-value:1.31E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:3.45|P-value:3.29E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.75|P-value:1.08E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:3.32|P-value:5.08E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-9.68|P-value:6.16E-22 ID:SGTC_867|Compound:0518-0118|FD-Score:3.34|P-value:4.79E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:5.12|P-value:2.10E-7 ID:SGTC_925|Compound:2064-1272|FD-Score:3.37|P-value:4.30E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.80|P-value:8.71E-5 ID:SGTC_1146|Compound:3579-1023|FD-Score:3.28|P-value:5.86E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.32|P-value:5.07E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:3.77|P-value:9.65E-5 ID:SGTC_1740|Compound:st038286|FD-Score:6.65|P-value:2.42E-11 ID:SGTC_1741|Compound:st038134|FD-Score:-4.63|P-value:2.41E-6 ID:SGTC_1744|Compound:st037937|FD-Score:5.05|P-value:3.05E-7 ID:SGTC_1773|Compound:st048542|FD-Score:4.16|P-value:1.97E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.60|P-value:1.90E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.25|P-value:6.67E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.91|P-value:5.62E-5 ID:SGTC_1794|Compound:st048918|FD-Score:5.13|P-value:1.94E-7 ID:SGTC_1796|Compound:st049173|FD-Score:3.67|P-value:1.42E-4 ID:SGTC_1986|Compound:st071812|FD-Score:3.30|P-value:5.52E-4 ID:SGTC_2013|Compound:4024628|FD-Score:-3.31|P-value:5.48E-4 ID:SGTC_2033|Compound:5192550|FD-Score:4.28|P-value:1.16E-5 ID:SGTC_2088|Compound:5278280|FD-Score:3.20|P-value:7.69E-4 ID:SGTC_2198|Compound:6589944|FD-Score:3.44|P-value:3.32E-4 ID:SGTC_2300|Compound:7976697|FD-Score:3.42|P-value:3.56E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.43|P-value:3.49E-4 ID:SGTC_2479|Compound:5617353|FD-Score:3.13|P-value:9.92E-4 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.40|P-value:3.88E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.90|P-value:5.88E-5 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.44|P-value:3.37E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.32|P-value:5.08E-4 ID:SGTC_2628|Compound:lathosterol|FD-Score:5.15|P-value:1.80E-7 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.56|P-value:3.30E-6 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.63|P-value:2.36E-6 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.85|P-value:7.14E-5 ID:SGTC_2792|Compound:7535235|FD-Score:-5.47|P-value:3.32E-8 ID:SGTC_2936|Compound:9038235|FD-Score:3.35|P-value:4.70E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.38|P-value:4.11E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.28|P-value:5.83E-4 ID:SGTC_3352|Compound:9151559|FD-Score:4.44|P-value:5.76E-6

Top fitness defect scores for YGL090W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1740 st038286 6.65 13.2 µM 2.42E-11 2 1.42E-11
2 SGTC_2628 lathosterol 5.15 100.0 µM 1.80E-7 10 1.31E-7
3 SGTC_1794 st048918 5.13 76.9 µM 1.94E-7 3 1.41E-7
4 SGTC_912 3474-0017 5.12 2.2 µM 2.10E-7 11 1.53E-7
5 SGTC_1744 st037937 5.05 38.9 µM 3.05E-7 5 2.24E-7
6 SGTC_2764 methyldopa 4.63 94.7 µM 2.36E-6 11 1.82E-6
7 SGTC_39 0986-0283 4.57 58.4 µM 3.13E-6 calcium & mitochondrial duress 18 2.43E-6
8 SGTC_2751 anetholtrithion 4.56 17.2 µM 3.30E-6 15 2.56E-6
9 SGTC_3352 9151559 4.44 45.6 µM 5.76E-6 6 4.54E-6
10 SGTC_45 0kpi-0036 4.37 14.9 µM 7.66E-6 excess fatty acid 12 6.07E-6
11 SGTC_2033 5192550 4.28 145.0 µM 1.16E-5 9 9.30E-6
12 SGTC_1773 st048542 4.16 73.6 µM 1.97E-5 36 1.60E-5
13 SGTC_359 0081-0058 3.94 15.9 µM 4.86E-5 60S ribosome export 43 4.02E-5
14 SGTC_550 4123-0385 3.93 25.7 µM 5.12E-5 TSC3-RPN4 23 4.24E-5
15 SGTC_2765 myclobutanil 3.85 7.7 µM 7.14E-5 azole & statin 50 5.96E-5
16 SGTC_1022 4477-0945 3.80 21.3 µM 8.71E-5 30 7.31E-5
17 SGTC_125 0133-0007 3.80 11.8 µM 8.77E-5 DNA intercalators 10 7.36E-5
18 SGTC_1533 dihydroergotamine mesylate 3.77 29.4 µM 9.65E-5 DNA intercalators 15 8.11E-5
19 SGTC_575 k072-0246 3.69 11.9 µM 1.31E-4 26 1.11E-4
20 SGTC_1796 st049173 3.67 110.4 µM 1.42E-4 22 1.20E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.343 1.88E-93 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.326 6.82E-84 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.307 3.83E-74 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.291 2.25E-66 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.272 8.10E-58 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.253 5.68E-50 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.244 1.21E-46 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.235 3.55E-43 YLR034C SMF3 hom Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
0.227 1.35E-40 YNL103W MET4 het Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
0.225 7.36E-40 YOR014W RTS1 hom B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
0.224 1.69E-39 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.222 7.96E-39 YOR033C EXO1 hom 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress
0.213 8.79E-36 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.199 2.74E-31 YJR018W_d YJR018W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.194 1.04E-29 YKL034W TUL1 hom Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1