YGL107C / RMD9

Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL107C / RMD9

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:7.77|P-value:6.85E-14 ID:SGTC_70|Compound:0141-0289|FD-Score:4.48|P-value:9.91E-6 ID:SGTC_87|Compound:0100-0052|FD-Score:4.87|P-value:1.73E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:4.92|P-value:1.38E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:7.25|P-value:2.49E-12 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.44|P-value:5.04E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.44|P-value:5.26E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:4.11|P-value:4.53E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:24.40|P-value:5.48E-120 ID:SGTC_275|Compound:trichlorophene|FD-Score:10.40|P-value:2.51E-23 ID:SGTC_365|Compound:0121-0052|FD-Score:3.26|P-value:9.62E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:11.70|P-value:5.41E-29 ID:SGTC_594|Compound:1487-1584|FD-Score:3.73|P-value:1.91E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.36|P-value:6.60E-4 ID:SGTC_616|Compound:4092-0450|FD-Score:3.34|P-value:7.48E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:-4.42|P-value:1.22E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:-4.13|P-value:3.96E-5 ID:SGTC_962|Compound:1254-0300|FD-Score:3.39|P-value:6.24E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-4.14|P-value:3.77E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.83|P-value:1.35E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.28|P-value:9.14E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.27|P-value:9.26E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.61|P-value:2.84E-4 ID:SGTC_1635|Compound:st007035|FD-Score:8.76|P-value:3.60E-17 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.07|P-value:4.95E-5 ID:SGTC_1728|Compound:st035614|FD-Score:3.37|P-value:6.75E-4 ID:SGTC_1730|Compound:st033808|FD-Score:4.23|P-value:2.77E-5 ID:SGTC_1732|Compound:st034308|FD-Score:4.53|P-value:8.20E-6 ID:SGTC_1736|Compound:st036774|FD-Score:-3.40|P-value:5.85E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.44|P-value:5.29E-4 ID:SGTC_1819|Compound:st053448|FD-Score:3.34|P-value:7.50E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:3.76|P-value:1.72E-4 ID:SGTC_1911|Compound:st060208|FD-Score:5.73|P-value:2.38E-8 ID:SGTC_1914|Compound:st062367|FD-Score:7.11|P-value:6.07E-12 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:4.04|P-value:5.89E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.32|P-value:7.95E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:-4.05|P-value:5.48E-5 ID:SGTC_2803|Compound:7707263|FD-Score:3.67|P-value:2.35E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.16|P-value:3.55E-5 ID:SGTC_2956|Compound:9076196|FD-Score:3.37|P-value:6.76E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.99|P-value:7.33E-5 ID:SGTC_3314|Compound:9135860|FD-Score:-4.43|P-value:1.17E-5 ID:SGTC_3319|Compound:9138450|FD-Score:-3.52|P-value:3.80E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.77|P-value:6.85E-14 ID:SGTC_70|Compound:0141-0289|FD-Score:4.48|P-value:9.91E-6 ID:SGTC_87|Compound:0100-0052|FD-Score:4.87|P-value:1.73E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:4.92|P-value:1.38E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:7.25|P-value:2.49E-12 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.44|P-value:5.04E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.44|P-value:5.26E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:4.11|P-value:4.53E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:24.40|P-value:5.48E-120 ID:SGTC_275|Compound:trichlorophene|FD-Score:10.40|P-value:2.51E-23 ID:SGTC_365|Compound:0121-0052|FD-Score:3.26|P-value:9.62E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:11.70|P-value:5.41E-29 ID:SGTC_594|Compound:1487-1584|FD-Score:3.73|P-value:1.91E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.36|P-value:6.60E-4 ID:SGTC_616|Compound:4092-0450|FD-Score:3.34|P-value:7.48E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:-4.42|P-value:1.22E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:-4.13|P-value:3.96E-5 ID:SGTC_962|Compound:1254-0300|FD-Score:3.39|P-value:6.24E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-4.14|P-value:3.77E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.83|P-value:1.35E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:3.28|P-value:9.14E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.27|P-value:9.26E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:-3.61|P-value:2.84E-4 ID:SGTC_1635|Compound:st007035|FD-Score:8.76|P-value:3.60E-17 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.07|P-value:4.95E-5 ID:SGTC_1728|Compound:st035614|FD-Score:3.37|P-value:6.75E-4 ID:SGTC_1730|Compound:st033808|FD-Score:4.23|P-value:2.77E-5 ID:SGTC_1732|Compound:st034308|FD-Score:4.53|P-value:8.20E-6 ID:SGTC_1736|Compound:st036774|FD-Score:-3.40|P-value:5.85E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.44|P-value:5.29E-4 ID:SGTC_1819|Compound:st053448|FD-Score:3.34|P-value:7.50E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:3.76|P-value:1.72E-4 ID:SGTC_1911|Compound:st060208|FD-Score:5.73|P-value:2.38E-8 ID:SGTC_1914|Compound:st062367|FD-Score:7.11|P-value:6.07E-12 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:4.04|P-value:5.89E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.32|P-value:7.95E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:-4.05|P-value:5.48E-5 ID:SGTC_2803|Compound:7707263|FD-Score:3.67|P-value:2.35E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.16|P-value:3.55E-5 ID:SGTC_2956|Compound:9076196|FD-Score:3.37|P-value:6.76E-4 ID:SGTC_3304|Compound:9125678|FD-Score:3.99|P-value:7.33E-5 ID:SGTC_3314|Compound:9135860|FD-Score:-4.43|P-value:1.17E-5 ID:SGTC_3319|Compound:9138450|FD-Score:-3.52|P-value:3.80E-4

Top fitness defect scores for YGL107C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 24.40 5.3 µM 5.48E-120 35 9.15E-132
2 SGTC_386 1509-0011 11.70 3.1 µM 5.41E-29 36 1.02E-31
3 SGTC_275 trichlorophene 10.40 5.7 µM 2.51E-23 mitochondrial stress 41 1.74E-25
4 SGTC_1635 st007035 8.76 42.4 µM 3.60E-17 92 1.01E-18
5 SGTC_58 0957-0393 7.77 9.0 µM 6.85E-14 cell wall 134 4.06E-15
6 SGTC_134 0269-0018 7.25 6.9 µM 2.49E-12 64 2.11E-13
7 SGTC_1914 st062367 7.11 72.6 µM 6.07E-12 2 5.61E-13
8 SGTC_1911 st060208 5.73 74.3 µM 2.38E-8 6 4.96E-9
9 SGTC_119 0121-0043 4.92 24.8 µM 1.38E-6 mitochondrial stress 60 4.26E-7
10 SGTC_87 0100-0052 4.87 35.5 µM 1.73E-6 60 5.47E-7
11 SGTC_1732 st034308 4.53 51.8 µM 8.20E-6 16 3.01E-6
12 SGTC_70 0141-0289 4.48 43.3 µM 9.91E-6 mitochondrial stress 139 3.71E-6
13 SGTC_1730 st033808 4.23 45.1 µM 2.77E-5 22 1.14E-5
14 SGTC_214 4373-3496 4.11 70.0 µM 4.53E-5 53 1.97E-5
15 SGTC_2454 5-benzylidenerhodanine 4.04 10.4 µM 5.89E-5 TRP & mitochondrial translation 36 2.62E-5
16 SGTC_3304 9125678 3.99 46.1 µM 7.33E-5 48 3.33E-5
17 SGTC_1108 betulinic acid 3.83 729.1 nM 1.35E-4 17 6.50E-5
18 SGTC_1823 3-hydroxy-2-naphthoic acid 3.76 106.0 µM 1.72E-4 22 8.49E-5
19 SGTC_594 1487-1584 3.73 10.8 µM 1.91E-4 31 9.49E-5
20 SGTC_2803 7707263 3.67 71.4 µM 2.35E-4 33 1.19E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.674 0 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.646 0 YJL022W_d YJL022W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.636 0 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.632 0 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.631 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.631 0 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.625 0 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.605 0 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.602 0 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.596 1.98E-322 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.585 5.59E-307 YDR231C COX20 hom Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.583 6.25E-305 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.579 2.93E-300 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.579 1.28E-299 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.578 5.87E-298 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs