YGL113W / SLD3

Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGL113W / SLD3

Click on Significant Values for Screen Details ID:SGTC_96|Compound:0929-0093|FD-Score:3.94|P-value:1.49E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:4.37|P-value:3.03E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:8.57|P-value:2.45E-15 ID:SGTC_197|Compound:4544-0043|FD-Score:5.49|P-value:2.51E-7 ID:SGTC_227|Compound:fluconazole|FD-Score:5.98|P-value:2.18E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.72|P-value:7.65E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:-4.09|P-value:1.12E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.96|P-value:1.37E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:3.52|P-value:6.16E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-5.99|P-value:2.94E-8 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.04|P-value:1.32E-4 ID:SGTC_389|Compound:tpck|FD-Score:4.16|P-value:6.77E-5 ID:SGTC_432|Compound:NS 1619|FD-Score:4.26|P-value:4.65E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.33|P-value:3.56E-9 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.21|P-value:7.34E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:-3.68|P-value:4.60E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-4.55|P-value:2.01E-5 ID:SGTC_576|Compound:1073-0113|FD-Score:3.97|P-value:1.35E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:3.53|P-value:5.98E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:5.88|P-value:3.78E-8 ID:SGTC_908|Compound:3448-7491|FD-Score:4.51|P-value:1.79E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:4.57|P-value:1.43E-5 ID:SGTC_919|Compound:tnp00227|FD-Score:4.87|P-value:4.13E-6 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.72|P-value:3.20E-4 ID:SGTC_1054|Compound:3429-0080|FD-Score:4.56|P-value:1.43E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.65|P-value:1.03E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:5.00|P-value:2.31E-6 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.39|P-value:9.13E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:-3.45|P-value:9.51E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.64|P-value:4.21E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:5.60|P-value:1.46E-7 ID:SGTC_1568|Compound:digitonin|FD-Score:-4.40|P-value:3.55E-5 ID:SGTC_1626|Compound:st003712|FD-Score:-5.30|P-value:8.39E-7 ID:SGTC_1712|Compound:st032288|FD-Score:4.97|P-value:2.61E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-4.78|P-value:7.93E-6 ID:SGTC_1773|Compound:st048542|FD-Score:3.65|P-value:4.07E-4 ID:SGTC_1774|Compound:st048371|FD-Score:-3.79|P-value:3.21E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:11.00|P-value:7.25E-24 ID:SGTC_1897|Compound:st060201|FD-Score:3.86|P-value:1.95E-4 ID:SGTC_1941|Compound:st076221|FD-Score:5.24|P-value:8.13E-7 ID:SGTC_1992|Compound:st073643|FD-Score:3.54|P-value:5.64E-4 ID:SGTC_2003|Compound:4023581|FD-Score:3.39|P-value:9.21E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.34|P-value:4.94E-7 ID:SGTC_2054|Compound:5233173|FD-Score:-5.90|P-value:4.70E-8 ID:SGTC_2269|Compound:7935389|FD-Score:3.52|P-value:6.19E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-4.04|P-value:1.35E-4 ID:SGTC_2322|Compound:7413319|FD-Score:12.10|P-value:8.04E-29 ID:SGTC_2413|Compound:inermin|FD-Score:18.00|P-value:8.04E-61 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.68|P-value:9.04E-6 ID:SGTC_2425|Compound:5628681|FD-Score:-3.61|P-value:5.76E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-4.21|P-value:7.19E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.64|P-value:5.17E-4 ID:SGTC_2570|Compound:licareol|FD-Score:3.72|P-value:3.17E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-4.55|P-value:1.96E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-4.48|P-value:2.67E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.83|P-value:2.18E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:6.24|P-value:5.69E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.66|P-value:4.80E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.99|P-value:1.23E-4 ID:SGTC_2991|Compound:9074669|FD-Score:4.04|P-value:1.04E-4 ID:SGTC_3036|Compound:9090181|FD-Score:-3.68|P-value:4.62E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:3.94|P-value:1.49E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:4.37|P-value:3.03E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:8.57|P-value:2.45E-15 ID:SGTC_197|Compound:4544-0043|FD-Score:5.49|P-value:2.51E-7 ID:SGTC_227|Compound:fluconazole|FD-Score:5.98|P-value:2.18E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.72|P-value:7.65E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:-4.09|P-value:1.12E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.96|P-value:1.37E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:3.52|P-value:6.16E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-5.99|P-value:2.94E-8 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.04|P-value:1.32E-4 ID:SGTC_389|Compound:tpck|FD-Score:4.16|P-value:6.77E-5 ID:SGTC_432|Compound:NS 1619|FD-Score:4.26|P-value:4.65E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.33|P-value:3.56E-9 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.21|P-value:7.34E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:-3.68|P-value:4.60E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-4.55|P-value:2.01E-5 ID:SGTC_576|Compound:1073-0113|FD-Score:3.97|P-value:1.35E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:3.53|P-value:5.98E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:5.88|P-value:3.78E-8 ID:SGTC_908|Compound:3448-7491|FD-Score:4.51|P-value:1.79E-5 ID:SGTC_911|Compound:3456-4043|FD-Score:4.57|P-value:1.43E-5 ID:SGTC_919|Compound:tnp00227|FD-Score:4.87|P-value:4.13E-6 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.72|P-value:3.20E-4 ID:SGTC_1054|Compound:3429-0080|FD-Score:4.56|P-value:1.43E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.65|P-value:1.03E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:5.00|P-value:2.31E-6 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.39|P-value:9.13E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:-3.45|P-value:9.51E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.64|P-value:4.21E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:5.60|P-value:1.46E-7 ID:SGTC_1568|Compound:digitonin|FD-Score:-4.40|P-value:3.55E-5 ID:SGTC_1626|Compound:st003712|FD-Score:-5.30|P-value:8.39E-7 ID:SGTC_1712|Compound:st032288|FD-Score:4.97|P-value:2.61E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-4.78|P-value:7.93E-6 ID:SGTC_1773|Compound:st048542|FD-Score:3.65|P-value:4.07E-4 ID:SGTC_1774|Compound:st048371|FD-Score:-3.79|P-value:3.21E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:11.00|P-value:7.25E-24 ID:SGTC_1897|Compound:st060201|FD-Score:3.86|P-value:1.95E-4 ID:SGTC_1941|Compound:st076221|FD-Score:5.24|P-value:8.13E-7 ID:SGTC_1992|Compound:st073643|FD-Score:3.54|P-value:5.64E-4 ID:SGTC_2003|Compound:4023581|FD-Score:3.39|P-value:9.21E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.34|P-value:4.94E-7 ID:SGTC_2054|Compound:5233173|FD-Score:-5.90|P-value:4.70E-8 ID:SGTC_2269|Compound:7935389|FD-Score:3.52|P-value:6.19E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-4.04|P-value:1.35E-4 ID:SGTC_2322|Compound:7413319|FD-Score:12.10|P-value:8.04E-29 ID:SGTC_2413|Compound:inermin|FD-Score:18.00|P-value:8.04E-61 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.68|P-value:9.04E-6 ID:SGTC_2425|Compound:5628681|FD-Score:-3.61|P-value:5.76E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-4.21|P-value:7.19E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:-3.64|P-value:5.17E-4 ID:SGTC_2570|Compound:licareol|FD-Score:3.72|P-value:3.17E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:-4.55|P-value:1.96E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-4.48|P-value:2.67E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.83|P-value:2.18E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:6.24|P-value:5.69E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.66|P-value:4.80E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.99|P-value:1.23E-4 ID:SGTC_2991|Compound:9074669|FD-Score:4.04|P-value:1.04E-4 ID:SGTC_3036|Compound:9090181|FD-Score:-3.68|P-value:4.62E-4

Top fitness defect scores for YGL113W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2413 inermin 18.00 26.1 µM 8.04E-61 4 9.24E-73 1.79
2 SGTC_2322 7413319 12.10 38.2 µM 8.04E-29 ERAD & cell cycle 1 3.18E-34 1.81
3 SGTC_1844 rapamycin 11.00 4.0 nM 7.25E-24 8 2.90E-28 0.47
4 SGTC_168 3970-0862 8.57 530.5 µM 2.45E-15 6 5.12E-18 0.07
5 SGTC_445 e6 berbamine 6.33 66.0 µM 3.56E-9 NEO1-PIK1 13 1.24E-10 0.02
6 SGTC_2662 daunorubicin 6.24 22.4 µM 5.69E-9 anthracycline transcription coupled DNA repair 8 2.18E-10 0.41
7 SGTC_227 fluconazole 5.98 33.5 µM 2.18E-8 azole & statin 29 1.09E-9 0.03
8 SGTC_902 1120-0002 5.88 2.1 µM 3.78E-8 azole & statin 7 2.10E-9 0.05
9 SGTC_1496 4469-0713 5.60 57.4 µM 1.46E-7 1 1.06E-8 0.85
10 SGTC_197 4544-0043 5.49 41.6 µM 2.51E-7 endomembrane recycling 13 2.01E-8 0.23
11 SGTC_2021 4019055 5.34 173.0 µM 4.94E-7 endomembrane recycling 23 4.52E-8 0.16
12 SGTC_1941 st076221 5.24 61.8 µM 8.13E-7 sphingolipid biosynthesis & PDR1 3 8.20E-8 0.19
13 SGTC_1380 2291-0730 5.00 164.0 µM 2.31E-6 2 2.85E-7 0.04
14 SGTC_1712 st032288 4.97 53.4 µM 2.61E-6 calcium & mitochondrial duress 24 3.29E-7 0.01
15 SGTC_919 tnp00227 4.87 403.6 nM 4.13E-6 RNA pol III & RNase P/MRP 7 5.69E-7 0.03
16 SGTC_234 nsc-17383 4.72 10.7 µM 7.65E-6 ERG2 3 1.19E-6 0.72
17 SGTC_2418 pentamidine 4.68 31.7 µM 9.04E-6 mitochondrial processes 12 1.45E-6 0.46
18 SGTC_1371 2421-0008 4.65 32.7 µM 1.03E-5 mitochondrial processes 18 1.69E-6 0.11
19 SGTC_911 3456-4043 4.57 236.0 µM 1.43E-5 mitochondrial processes 5 2.49E-6 0.06
20 SGTC_1054 3429-0080 4.56 167.0 µM 1.43E-5 4 2.51E-6 0.59

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.304 6.76E-73 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.203 1.05E-32 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.197 8.09E-31 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.197 1.55E-30 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.190 1.29E-28 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.183 1.15E-26 YOR132W VPS17 hom Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
0.178 3.20E-25 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.165 5.63E-22 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.163 2.24E-21 YGR098C ESP1 het Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.161 8.25E-21 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.158 3.59E-20 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.156 1.14E-19 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.151 1.23E-18 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.149 3.83E-18 YMR043W MCM1 het Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
0.147 9.76E-18 YPL112C PEX25 hom Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p