YGL158W / RCK1

Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL158W / RCK1

Click on Significant Values for Screen Details ID:SGTC_170|Compound:4197-0004|FD-Score:-2.92|P-value:5.46E-4 ID:SGTC_180|Compound:1486-1293|FD-Score:3.16|P-value:2.24E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.30|P-value:1.22E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-6.29|P-value:1.08E-12 ID:SGTC_325|Compound:2810-4341|FD-Score:-4.08|P-value:2.60E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:3.01|P-value:4.02E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.70|P-value:1.88E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:3.16|P-value:2.16E-4 ID:SGTC_394|Compound:menadione|FD-Score:-4.39|P-value:4.74E-7 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.12|P-value:2.38E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.33|P-value:1.06E-4 ID:SGTC_458|Compound:nppb|FD-Score:-4.19|P-value:1.43E-6 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.17|P-value:1.98E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.73|P-value:1.70E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:3.61|P-value:2.96E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:4.06|P-value:3.12E-6 ID:SGTC_918|Compound:glabranin|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:2.88|P-value:6.73E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.37|P-value:5.61E-7 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.56|P-value:3.82E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:-2.97|P-value:4.46E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-5.51|P-value:3.71E-10 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.86|P-value:7.95E-6 ID:SGTC_1112|Compound:tnp00069|FD-Score:-7.10|P-value:1.10E-15 ID:SGTC_1191|Compound:1487-1166|FD-Score:-2.83|P-value:7.84E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:2.91|P-value:6.02E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:2.78|P-value:9.93E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.89|P-value:7.61E-6 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-3.42|P-value:6.63E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:3.45|P-value:6.13E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-3.07|P-value:2.96E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.09|P-value:2.68E-4 ID:SGTC_1663|Compound:st014140|FD-Score:-2.92|P-value:5.33E-4 ID:SGTC_1689|Compound:st020265|FD-Score:3.09|P-value:3.00E-4 ID:SGTC_1728|Compound:st035614|FD-Score:5.11|P-value:6.50E-9 ID:SGTC_1737|Compound:st035557|FD-Score:-3.79|P-value:1.13E-5 ID:SGTC_1784|Compound:st039334|FD-Score:2.86|P-value:7.40E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.23|P-value:1.52E-4 ID:SGTC_2342|Compound:7990637|FD-Score:2.92|P-value:5.82E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-2.85|P-value:7.15E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:5.13|P-value:5.78E-9 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:5.18|P-value:4.06E-9 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-2.91|P-value:5.74E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.71|P-value:8.07E-8 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.72|P-value:1.78E-5 ID:SGTC_2783|Compound:7738165|FD-Score:3.12|P-value:2.58E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.36|P-value:9.27E-5 ID:SGTC_2819|Compound:7996655|FD-Score:3.00|P-value:4.20E-4 ID:SGTC_2823|Compound:7987624|FD-Score:4.77|P-value:5.38E-8 ID:SGTC_2943|Compound:9068529|FD-Score:-3.74|P-value:1.42E-5 ID:SGTC_2946|Compound:9065502|FD-Score:3.96|P-value:5.16E-6 ID:SGTC_2950|Compound:9072895|FD-Score:-6.62|P-value:6.85E-14 ID:SGTC_2952|Compound:9073731|FD-Score:-3.20|P-value:1.75E-4 ID:SGTC_2958|Compound:9077254|FD-Score:4.34|P-value:6.73E-7 ID:SGTC_2962|Compound:9082311|FD-Score:-5.85|P-value:3.29E-11 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.18|P-value:2.04E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.86|P-value:3.25E-8 ID:SGTC_3206|Compound:9114350|FD-Score:3.61|P-value:2.94E-5 ID:SGTC_3210|Compound:9128157|FD-Score:4.24|P-value:1.17E-6 ID:SGTC_170|Compound:4197-0004|FD-Score:-2.92|P-value:5.46E-4 ID:SGTC_180|Compound:1486-1293|FD-Score:3.16|P-value:2.24E-4 ID:SGTC_277|Compound:nystatin|FD-Score:3.30|P-value:1.22E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-6.29|P-value:1.08E-12 ID:SGTC_325|Compound:2810-4341|FD-Score:-4.08|P-value:2.60E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:3.01|P-value:4.02E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.70|P-value:1.88E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:3.16|P-value:2.16E-4 ID:SGTC_394|Compound:menadione|FD-Score:-4.39|P-value:4.74E-7 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:-3.12|P-value:2.38E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.33|P-value:1.06E-4 ID:SGTC_458|Compound:nppb|FD-Score:-4.19|P-value:1.43E-6 ID:SGTC_631|Compound:k072-0230|FD-Score:-3.17|P-value:1.98E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.73|P-value:1.70E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:3.61|P-value:2.96E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:4.06|P-value:3.12E-6 ID:SGTC_918|Compound:glabranin|FD-Score:3.06|P-value:3.38E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:2.88|P-value:6.73E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.37|P-value:5.61E-7 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.56|P-value:3.82E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:-2.97|P-value:4.46E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-5.51|P-value:3.71E-10 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.86|P-value:7.95E-6 ID:SGTC_1112|Compound:tnp00069|FD-Score:-7.10|P-value:1.10E-15 ID:SGTC_1191|Compound:1487-1166|FD-Score:-2.83|P-value:7.84E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:2.91|P-value:6.02E-4 ID:SGTC_1210|Compound:3013-0140|FD-Score:2.78|P-value:9.93E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.89|P-value:7.61E-6 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-3.42|P-value:6.63E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:3.45|P-value:6.13E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-3.07|P-value:2.96E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.09|P-value:2.68E-4 ID:SGTC_1663|Compound:st014140|FD-Score:-2.92|P-value:5.33E-4 ID:SGTC_1689|Compound:st020265|FD-Score:3.09|P-value:3.00E-4 ID:SGTC_1728|Compound:st035614|FD-Score:5.11|P-value:6.50E-9 ID:SGTC_1737|Compound:st035557|FD-Score:-3.79|P-value:1.13E-5 ID:SGTC_1784|Compound:st039334|FD-Score:2.86|P-value:7.40E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.23|P-value:1.52E-4 ID:SGTC_2342|Compound:7990637|FD-Score:2.92|P-value:5.82E-4 ID:SGTC_2449|Compound:5812982|FD-Score:-2.85|P-value:7.15E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:5.13|P-value:5.78E-9 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:5.18|P-value:4.06E-9 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-2.91|P-value:5.74E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:4.71|P-value:8.07E-8 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.72|P-value:1.78E-5 ID:SGTC_2783|Compound:7738165|FD-Score:3.12|P-value:2.58E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.36|P-value:9.27E-5 ID:SGTC_2819|Compound:7996655|FD-Score:3.00|P-value:4.20E-4 ID:SGTC_2823|Compound:7987624|FD-Score:4.77|P-value:5.38E-8 ID:SGTC_2943|Compound:9068529|FD-Score:-3.74|P-value:1.42E-5 ID:SGTC_2946|Compound:9065502|FD-Score:3.96|P-value:5.16E-6 ID:SGTC_2950|Compound:9072895|FD-Score:-6.62|P-value:6.85E-14 ID:SGTC_2952|Compound:9073731|FD-Score:-3.20|P-value:1.75E-4 ID:SGTC_2958|Compound:9077254|FD-Score:4.34|P-value:6.73E-7 ID:SGTC_2962|Compound:9082311|FD-Score:-5.85|P-value:3.29E-11 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.18|P-value:2.04E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.86|P-value:3.25E-8 ID:SGTC_3206|Compound:9114350|FD-Score:3.61|P-value:2.94E-5 ID:SGTC_3210|Compound:9128157|FD-Score:4.24|P-value:1.17E-6

Top fitness defect scores for YGL158W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2633 5,7-dihydroxyisoflavone 5.18 100.0 µM 4.06E-9 14 1.11E-7
2 SGTC_2596 sinensetin 5.13 100.0 µM 5.78E-9 10 1.48E-7
3 SGTC_1728 st035614 5.11 33.8 µM 6.50E-9 18 1.63E-7
4 SGTC_3202 9113761 4.86 49.5 µM 3.25E-8 23 6.02E-7
5 SGTC_2823 7987624 4.77 58.4 µM 5.38E-8 11 9.06E-7
6 SGTC_2638 epoxomicin 4.71 18.0 µM 8.07E-8 18 1.26E-6
7 SGTC_978 1416-0512 4.37 87.4 µM 5.61E-7 redox potentiating 23 6.08E-6
8 SGTC_2958 9077254 4.34 16.7 µM 6.73E-7 21 7.04E-6
9 SGTC_3210 9128157 4.24 49.5 µM 1.17E-6 40 1.11E-5
10 SGTC_866 0495-0101 4.06 262.0 µM 3.12E-6 60S ribosome export 16 2.45E-5
11 SGTC_2946 9065502 3.96 71.4 µM 5.16E-6 7 3.69E-5
12 SGTC_1345 1488-0192 3.89 31.4 µM 7.61E-6 16 5.06E-5
13 SGTC_763 3453-1109 3.73 128.0 µM 1.70E-5 23 9.75E-5
14 SGTC_2753 fluvastatin 3.72 17.1 µM 1.78E-5 azole & statin 45 1.01E-4
15 SGTC_354 2783-3500 3.70 4.9 µM 1.88E-5 cell wall 54 1.06E-4
16 SGTC_3206 9114350 3.61 49.5 µM 2.94E-5 26 1.52E-4
17 SGTC_769 0302-0231 3.61 76.0 µM 2.96E-5 56 1.53E-4
18 SGTC_1013 4112-3890 3.56 241.0 µM 3.82E-5 TSC3-RPN4 47 1.89E-4
19 SGTC_1581 himbacine 3.45 57.9 µM 6.13E-5 Golgi 60 2.77E-4
20 SGTC_2816 7982706 3.36 58.4 µM 9.27E-5 azole & statin 82 3.88E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.202 3.63E-32 YOR286W RDL2 hom Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
0.168 1.20E-22 YJL130C URA2 hom Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
0.157 5.27E-20 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.110 1.64E-10 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.108 4.07E-10 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.107 5.22E-10 YNL305C BXI1 hom Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
0.098 1.33E-8 YMR017W SPO20 hom Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
0.097 1.68E-8 YGR035W-A_p YGR035W-A_p hom Putative protein of unknown function
0.095 3.53E-8 YIR005W IST3 hom Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing
0.094 4.27E-8 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.093 7.65E-8 YBL068W PRS4 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
0.091 1.36E-7 YLR392C ART10 hom Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
0.089 2.14E-7 YFR039C_p YFR039C_p hom Putative protein of unknown function; may be involved in response to high salt and changes in carbon source; deletion mutant has decreased spore survival in Drosophila feces
0.087 4.90E-7 YJL032W_d YJL032W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
0.085 8.71E-7 YDR259C YAP6 hom Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication