YGL163C / RAD54

DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL163C / RAD54

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:4.73|P-value:2.30E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:4.28|P-value:1.18E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:5.65|P-value:4.90E-7 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.58|P-value:3.92E-5 ID:SGTC_244|Compound:nsc-207895|FD-Score:9.78|P-value:4.95E-18 ID:SGTC_248|Compound:streptozotocin|FD-Score:5.05|P-value:6.50E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:6.15|P-value:4.66E-8 ID:SGTC_268|Compound:camptothecin|FD-Score:6.65|P-value:3.47E-9 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.23|P-value:1.38E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:4.73|P-value:2.27E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:6.70|P-value:2.73E-9 ID:SGTC_558|Compound:k064-0027|FD-Score:5.61|P-value:5.78E-7 ID:SGTC_620|Compound:0109-0052|FD-Score:4.21|P-value:1.49E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.80|P-value:5.84E-4 ID:SGTC_707|Compound:0865-0008|FD-Score:3.91|P-value:4.01E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:4.35|P-value:9.23E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:4.83|P-value:1.54E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:10.30|P-value:7.19E-20 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.00|P-value:3.03E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.57|P-value:4.63E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.60|P-value:4.24E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:5.48|P-value:1.03E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:5.16|P-value:4.02E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:4.34|P-value:9.46E-5 ID:SGTC_1079|Compound:idarubicin|FD-Score:6.42|P-value:1.18E-8 ID:SGTC_1082|Compound:camptothecin|FD-Score:7.12|P-value:2.68E-10 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.40|P-value:7.80E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.56|P-value:4.82E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:-4.35|P-value:3.15E-5 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.80|P-value:5.68E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-4.12|P-value:7.28E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.71|P-value:7.54E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:4.23|P-value:1.38E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:5.19|P-value:3.64E-6 ID:SGTC_1466|Compound:k213-0079|FD-Score:3.84|P-value:5.13E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:6.31|P-value:2.04E-8 ID:SGTC_1616|Compound:st002734|FD-Score:-3.78|P-value:2.32E-4 ID:SGTC_1627|Compound:st003713|FD-Score:5.58|P-value:6.63E-7 ID:SGTC_1682|Compound:st012968|FD-Score:-3.53|P-value:5.32E-4 ID:SGTC_1703|Compound:st026983|FD-Score:6.43|P-value:1.10E-8 ID:SGTC_1707|Compound:st032323|FD-Score:11.30|P-value:1.78E-23 ID:SGTC_1718|Compound:roemerine|FD-Score:8.29|P-value:2.13E-13 ID:SGTC_1719|Compound:st032227|FD-Score:3.96|P-value:3.41E-4 ID:SGTC_1723|Compound:st034307|FD-Score:3.88|P-value:4.46E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.77|P-value:6.25E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.17|P-value:1.72E-4 ID:SGTC_1913|Compound:st060213|FD-Score:5.58|P-value:6.74E-7 ID:SGTC_1915|Compound:st067105|FD-Score:5.00|P-value:7.95E-6 ID:SGTC_1927|Compound:st060202|FD-Score:5.01|P-value:7.54E-6 ID:SGTC_1930|Compound:st074634|FD-Score:6.68|P-value:3.11E-9 ID:SGTC_1936|Compound:st074407|FD-Score:7.50|P-value:3.04E-11 ID:SGTC_1975|Compound:st070967|FD-Score:3.72|P-value:7.34E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.69|P-value:7.99E-4 ID:SGTC_2065|Compound:5221596|FD-Score:3.70|P-value:7.91E-4 ID:SGTC_2070|Compound:5230947|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_2152|Compound:5568786|FD-Score:3.64|P-value:9.37E-4 ID:SGTC_2375|Compound:9072683|FD-Score:-4.32|P-value:3.52E-5 ID:SGTC_2383|Compound:9036125|FD-Score:4.87|P-value:1.32E-5 ID:SGTC_2384|Compound:9036245|FD-Score:-3.75|P-value:2.58E-4 ID:SGTC_2390|Compound:9097792|FD-Score:-3.71|P-value:2.99E-4 ID:SGTC_2433|Compound:5607874|FD-Score:4.61|P-value:3.59E-5 ID:SGTC_2434|Compound:5670830|FD-Score:5.12|P-value:4.72E-6 ID:SGTC_2442|Compound:5158906|FD-Score:3.72|P-value:7.37E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.09|P-value:2.27E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:10.20|P-value:2.40E-19 ID:SGTC_2671|Compound:danthron|FD-Score:5.82|P-value:2.19E-7 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.67|P-value:8.52E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:5.02|P-value:7.36E-6 ID:SGTC_2945|Compound:9067681|FD-Score:3.84|P-value:5.01E-4 ID:SGTC_2947|Compound:9072639|FD-Score:8.15|P-value:5.26E-13 ID:SGTC_3028|Compound:9087778|FD-Score:6.79|P-value:1.72E-9 ID:SGTC_3031|Compound:9088762|FD-Score:3.80|P-value:5.72E-4 ID:SGTC_3039|Compound:9090270|FD-Score:-4.10|P-value:7.86E-5 ID:SGTC_3044|Compound:9093193|FD-Score:4.65|P-value:3.10E-5 ID:SGTC_3247|Compound:9135149|FD-Score:8.59|P-value:2.83E-14 ID:SGTC_3254|Compound:9136651|FD-Score:5.61|P-value:5.92E-7 ID:SGTC_73|Compound:0327-0325|FD-Score:4.73|P-value:2.30E-5 ID:SGTC_93|Compound:0109-0045|FD-Score:4.28|P-value:1.18E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:5.65|P-value:4.90E-7 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.58|P-value:3.92E-5 ID:SGTC_244|Compound:nsc-207895|FD-Score:9.78|P-value:4.95E-18 ID:SGTC_248|Compound:streptozotocin|FD-Score:5.05|P-value:6.50E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:6.15|P-value:4.66E-8 ID:SGTC_268|Compound:camptothecin|FD-Score:6.65|P-value:3.47E-9 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.23|P-value:1.38E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:4.73|P-value:2.27E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:6.70|P-value:2.73E-9 ID:SGTC_558|Compound:k064-0027|FD-Score:5.61|P-value:5.78E-7 ID:SGTC_620|Compound:0109-0052|FD-Score:4.21|P-value:1.49E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.80|P-value:5.84E-4 ID:SGTC_707|Compound:0865-0008|FD-Score:3.91|P-value:4.01E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:4.35|P-value:9.23E-5 ID:SGTC_824|Compound:1304-0008|FD-Score:4.83|P-value:1.54E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:10.30|P-value:7.19E-20 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.00|P-value:3.03E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.57|P-value:4.63E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.60|P-value:4.24E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:5.48|P-value:1.03E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:5.16|P-value:4.02E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:4.34|P-value:9.46E-5 ID:SGTC_1079|Compound:idarubicin|FD-Score:6.42|P-value:1.18E-8 ID:SGTC_1082|Compound:camptothecin|FD-Score:7.12|P-value:2.68E-10 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.40|P-value:7.80E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.56|P-value:4.82E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:-4.35|P-value:3.15E-5 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.80|P-value:5.68E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-4.12|P-value:7.28E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.71|P-value:7.54E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:4.23|P-value:1.38E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:5.19|P-value:3.64E-6 ID:SGTC_1466|Compound:k213-0079|FD-Score:3.84|P-value:5.13E-4 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:6.31|P-value:2.04E-8 ID:SGTC_1616|Compound:st002734|FD-Score:-3.78|P-value:2.32E-4 ID:SGTC_1627|Compound:st003713|FD-Score:5.58|P-value:6.63E-7 ID:SGTC_1682|Compound:st012968|FD-Score:-3.53|P-value:5.32E-4 ID:SGTC_1703|Compound:st026983|FD-Score:6.43|P-value:1.10E-8 ID:SGTC_1707|Compound:st032323|FD-Score:11.30|P-value:1.78E-23 ID:SGTC_1718|Compound:roemerine|FD-Score:8.29|P-value:2.13E-13 ID:SGTC_1719|Compound:st032227|FD-Score:3.96|P-value:3.41E-4 ID:SGTC_1723|Compound:st034307|FD-Score:3.88|P-value:4.46E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.77|P-value:6.25E-4 ID:SGTC_1845|Compound:st056753|FD-Score:4.17|P-value:1.72E-4 ID:SGTC_1913|Compound:st060213|FD-Score:5.58|P-value:6.74E-7 ID:SGTC_1915|Compound:st067105|FD-Score:5.00|P-value:7.95E-6 ID:SGTC_1927|Compound:st060202|FD-Score:5.01|P-value:7.54E-6 ID:SGTC_1930|Compound:st074634|FD-Score:6.68|P-value:3.11E-9 ID:SGTC_1936|Compound:st074407|FD-Score:7.50|P-value:3.04E-11 ID:SGTC_1975|Compound:st070967|FD-Score:3.72|P-value:7.34E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.69|P-value:7.99E-4 ID:SGTC_2065|Compound:5221596|FD-Score:3.70|P-value:7.91E-4 ID:SGTC_2070|Compound:5230947|FD-Score:4.21|P-value:1.50E-4 ID:SGTC_2152|Compound:5568786|FD-Score:3.64|P-value:9.37E-4 ID:SGTC_2375|Compound:9072683|FD-Score:-4.32|P-value:3.52E-5 ID:SGTC_2383|Compound:9036125|FD-Score:4.87|P-value:1.32E-5 ID:SGTC_2384|Compound:9036245|FD-Score:-3.75|P-value:2.58E-4 ID:SGTC_2390|Compound:9097792|FD-Score:-3.71|P-value:2.99E-4 ID:SGTC_2433|Compound:5607874|FD-Score:4.61|P-value:3.59E-5 ID:SGTC_2434|Compound:5670830|FD-Score:5.12|P-value:4.72E-6 ID:SGTC_2442|Compound:5158906|FD-Score:3.72|P-value:7.37E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.09|P-value:2.27E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:10.20|P-value:2.40E-19 ID:SGTC_2671|Compound:danthron|FD-Score:5.82|P-value:2.19E-7 ID:SGTC_2677|Compound:mitomycin C|FD-Score:3.67|P-value:8.52E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:5.02|P-value:7.36E-6 ID:SGTC_2945|Compound:9067681|FD-Score:3.84|P-value:5.01E-4 ID:SGTC_2947|Compound:9072639|FD-Score:8.15|P-value:5.26E-13 ID:SGTC_3028|Compound:9087778|FD-Score:6.79|P-value:1.72E-9 ID:SGTC_3031|Compound:9088762|FD-Score:3.80|P-value:5.72E-4 ID:SGTC_3039|Compound:9090270|FD-Score:-4.10|P-value:7.86E-5 ID:SGTC_3044|Compound:9093193|FD-Score:4.65|P-value:3.10E-5 ID:SGTC_3247|Compound:9135149|FD-Score:8.59|P-value:2.83E-14 ID:SGTC_3254|Compound:9136651|FD-Score:5.61|P-value:5.92E-7

Top fitness defect scores for YGL163C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1707 st032323 11.30 6.3 µM 1.78E-23 DNA damage response 8 7.61E-30
2 SGTC_829 1635-0120 10.30 117.0 µM 7.19E-20 DNA damage response 5 3.05E-25
3 SGTC_2612 dictamnine 10.20 15.5 µM 2.40E-19 RNA pol III & RNase P/MRP 2 1.42E-24
4 SGTC_244 nsc-207895 9.78 1.3 µM 4.95E-18 DNA damage response 4 6.80E-23
5 SGTC_3247 9135149 8.59 49.5 µM 2.83E-14 DNA damage response 4 4.32E-18
6 SGTC_1718 roemerine 8.29 11.9 µM 2.13E-13 redox potentiating 7 5.70E-17
7 SGTC_2947 9072639 8.15 7.7 µM 5.26E-13 tubulin folding & SWR complex 8 1.82E-16
8 SGTC_1936 st074407 7.50 74.3 µM 3.04E-11 DNA damage response 4 3.27E-14
9 SGTC_1082 camptothecin 7.12 424.9 nM 2.68E-10 DNA damage response 15 5.32E-13
10 SGTC_3028 9087778 6.79 49.5 µM 1.72E-9 2 5.78E-12
11 SGTC_429 1112-0049 6.70 25.5 µM 2.73E-9 DNA damage response 10 1.04E-11
12 SGTC_1930 st074634 6.68 34.3 µM 3.11E-9 RNA pol III & RNase P/MRP 6 1.23E-11
13 SGTC_268 camptothecin 6.65 39.6 µM 3.47E-9 DNA damage response 22 1.42E-11
14 SGTC_1703 st026983 6.43 48.3 µM 1.10E-8 1 6.20E-11
15 SGTC_1079 idarubicin 6.42 2.4 µM 1.18E-8 DNA damage response 5 6.78E-11
16 SGTC_1532 4',5'-dihydroxyflavone 6.31 78.7 µM 2.04E-8 Golgi 8 1.38E-10
17 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 6.15 44.4 nM 4.66E-8 DNA damage response 18 3.97E-10
18 SGTC_2671 danthron 5.82 100.0 µM 2.19E-7 DNA damage response 15 2.90E-9
19 SGTC_219 mitoxanthrone 5.65 164.4 µM 4.90E-7 DNA intercalators 20 8.14E-9
20 SGTC_558 k064-0027 5.61 21.3 µM 5.78E-7 DNA damage response 7 1.01E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.589 1.50E-312 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.561 2.55E-277 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.522 2.34E-234 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.415 4.13E-140 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.409 2.88E-135 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.392 1.84E-123 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.363 3.83E-105 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.329 1.17E-85 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.326 7.90E-84 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.307 5.39E-74 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.228 7.62E-41 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.223 5.91E-39 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.219 1.03E-37 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.202 3.34E-32 YDR369C XRS2 hom Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
0.193 1.20E-29 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress