HIPHOP chemogenomics database

YGL175C / SAE2

Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL175C / SAE2

Click on Significant Values for Screen Details

Top fitness defect scores for YGL175C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1707	st032323	15.20	6.3 µM	7.62E-55	DNA damage response	3	3.04E-52
2	SGTC_829	1635-0120	9.29	117.0 µM	6.94E-22	DNA damage response	7	7.82E-21
3	SGTC_1082	camptothecin	9.13	424.9 nM	3.26E-21	DNA damage response	6	3.41E-20
4	SGTC_3247	9135149	8.55	49.5 µM	7.39E-19	DNA damage response	5	5.97E-18
5	SGTC_268	camptothecin	8.44	39.6 µM	2.05E-18	DNA damage response	12	1.58E-17
6	SGTC_184	1494-0393	7.12	24.7 µM	1.16E-13		13	5.26E-13
7	SGTC_244	nsc-207895	6.83	1.3 µM	1.08E-12	DNA damage response	7	4.40E-12
8	SGTC_2638	epoxomicin	6.60	18.0 µM	5.41E-12		4	2.03E-11
9	SGTC_558	k064-0027	6.54	21.3 µM	8.20E-12	DNA damage response	5	3.02E-11
10	SGTC_174	3553-0389	5.93	74.3 µM	5.09E-10		5	1.53E-9
11	SGTC_1522	st012842	5.89	45.6 µM	6.62E-10	RNA pol III & RNase P/MRP	7	1.96E-9
12	SGTC_2463	5473429	5.67	200.0 µM	2.57E-9	ERG2	4	7.13E-9
13	SGTC_2671	danthron	5.43	100.0 µM	1.11E-8	DNA damage response	18	2.87E-8
14	SGTC_9	0092-0002	5.23	10.4 µM	3.49E-8		10	8.49E-8
15	SGTC_1079	idarubicin	5.21	2.4 µM	3.91E-8	DNA damage response	11	9.46E-8
16	SGTC_1718	roemerine	4.88	11.9 µM	2.44E-7	redox potentiating	24	5.39E-7
17	SGTC_381	3461-2219	4.67	42.7 µM	7.02E-7	copper-dependent oxidative stress	11	1.47E-6
18	SGTC_2932	9016711	4.67	44.5 µM	7.22E-7		29	1.51E-6
19	SGTC_238	5hr heat shock (37°C) + nsc-638432	4.66	8.9 µM	7.63E-7	heat shock/prefoldin	22	1.59E-6
20	SGTC_2302	7771753	4.63	200.0 µM	8.63E-7		25	1.79E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.536	8.01E-249	YDL059C	RAD59	hom	Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.468	3.32E-182	YDR076W	RAD55	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.423	7.24E-146	YDR386W	MUS81	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.416	1.32E-140	YDR004W	RAD57	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.400	6.90E-129	YBR099C_d	YBR099C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.392	1.84E-123	YGL163C	RAD54	hom	DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.381	2.24E-116	YBR098W	MMS4	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.364	2.06E-105	YJL092W	SRS2	hom	DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.329	1.21E-85	YOR191W	ULS1	hom	Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.268	2.86E-56	YDR075W	PPH3	hom	Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.264	1.40E-54	YMR048W	CSM3	hom	Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.246	2.04E-47	YER095W	RAD51	hom	Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.243	2.49E-46	YBL088C	TEL1	hom	Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.204	7.34E-33	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.179	1.64E-25	YNL273W	TOF1	hom	Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase