HIPHOP chemogenomics database

YGL246C / RAI1

Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z

Zygosity: Homozygous strain

fixedexpanded
Profile for YGL246C / RAI1

Click on Significant Values for Screen Details

Top fitness defect scores for YGL246C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_58	0957-0393	8.93	9.0 µM	4.76E-18	cell wall	101	2.19E-19
2	SGTC_832	4083-0019	8.15	164.0 µM	2.27E-15	translation	7	1.78E-16
3	SGTC_59	0330-0160	7.34	1.3 µM	8.12E-13		19	1.05E-13
4	SGTC_831	k061-1017	6.74	217.0 µM	4.38E-11	translation	7	7.97E-12
5	SGTC_800	3448-7362	6.32	155.0 µM	5.84E-10	translation	38	1.32E-10
6	SGTC_118	0083-0097	6.27	135.3 µM	7.57E-10	translation	15	1.75E-10
7	SGTC_830	4076-0286	6.11	57.8 µM	2.01E-9	translation	15	5.04E-10
8	SGTC_241	5hr heat shock (37°C) + cantharidin	5.65	155.4 µM	2.56E-8	cell wall signaling	37	7.94E-9
9	SGTC_56	0957-0365	5.62	83.2 µM	2.98E-8	Golgi	19	9.35E-9
10	SGTC_2809	7964133	5.54	13.0 µM	4.62E-8	60S ribosome export	9	1.50E-8
11	SGTC_3193	9111931	5.54	49.5 µM	4.76E-8		5	1.55E-8
12	SGTC_3206	9114350	5.53	49.5 µM	4.87E-8		3	1.59E-8
13	SGTC_2688	kanamycin b	5.47	96.7 µM	6.80E-8	calcium & mitochondrial duress	198	2.28E-8
14	SGTC_1750	st081859	5.00	2.4 µM	6.90E-7	endomembrane recycling	138	2.80E-7
15	SGTC_2095	5432005	4.92	200.0 µM	1.04E-6		4	4.35E-7
16	SGTC_1007	4101-0031	4.61	70.0 µM	4.22E-6		8	1.98E-6
17	SGTC_2431	5490079	4.50	200.0 µM	7.08E-6		19	3.46E-6
18	SGTC_103	0844-0104	4.48	51.8 µM	7.73E-6	ERAD & cell cycle	120	3.81E-6
19	SGTC_385	1494-0393	4.35	12.4 µM	1.32E-5		13	6.78E-6
20	SGTC_1024	4507-0395	4.31	26.0 µM	1.60E-5	TSC3-RPN4	19	8.34E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.272	9.27E-58	YLR048W	RPS0B	hom	Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.255	7.53E-51	YEL054C	RPL12A	hom	Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.219	1.33E-37	YDR140W	MTQ2	hom	S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.216	1.36E-36	YJL189W	RPL39	hom	Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.207	1.27E-33	YLR061W	RPL22A	hom	Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.190	1.40E-28	YML021C	UNG1	hom	Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.188	7.86E-28	YLR407W_p	YLR407W_p	hom	Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
0.182	2.58E-26	YBR267W	REI1	hom	Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.180	1.28E-25	YJL188C_d	BUD19_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.179	1.57E-25	YGR271C-A	EFG1	hom	Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.178	3.07E-25	YCR085W_d	YCR085W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.177	8.08E-25	YML061C	PIF1	hom	DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
0.175	2.50E-24	YOR309C_d	YOR309C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.173	6.03E-24	YGL135W	RPL1B	hom	Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
0.169	1.10E-22	YGR165W	MRPS35	hom	Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets