YGL246C / RAI1

Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL246C / RAI1

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:5.62|P-value:2.98E-8 ID:SGTC_57|Compound:miconazole|FD-Score:3.32|P-value:6.40E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:8.93|P-value:4.76E-18 ID:SGTC_59|Compound:0330-0160|FD-Score:7.34|P-value:8.12E-13 ID:SGTC_62|Compound:0180-0423|FD-Score:3.39|P-value:5.17E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.76|P-value:3.22E-6 ID:SGTC_87|Compound:0100-0052|FD-Score:3.27|P-value:7.76E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.48|P-value:7.73E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.29|P-value:9.39E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:6.27|P-value:7.57E-10 ID:SGTC_176|Compound:1486-1328|FD-Score:3.25|P-value:8.05E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.65|P-value:2.56E-8 ID:SGTC_285|Compound:1486-1325|FD-Score:3.73|P-value:1.55E-4 ID:SGTC_296|Compound:1222-0039|FD-Score:3.29|P-value:7.13E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:4.35|P-value:1.32E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:4.05|P-value:4.44E-5 ID:SGTC_527|Compound:4476-4215|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.40|P-value:4.97E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.60|P-value:2.49E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:6.32|P-value:5.84E-10 ID:SGTC_829|Compound:1635-0120|FD-Score:-4.19|P-value:3.62E-5 ID:SGTC_830|Compound:4076-0286|FD-Score:6.11|P-value:2.01E-9 ID:SGTC_831|Compound:k061-1017|FD-Score:6.74|P-value:4.38E-11 ID:SGTC_832|Compound:4083-0019|FD-Score:8.15|P-value:2.27E-15 ID:SGTC_879|Compound:0831-1030|FD-Score:4.24|P-value:2.08E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:4.61|P-value:4.22E-6 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.31|P-value:1.60E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-10.50|P-value:8.86E-24 ID:SGTC_1109|Compound:tnp00067|FD-Score:-4.28|P-value:2.57E-5 ID:SGTC_1111|Compound:tnp00062|FD-Score:-8.14|P-value:4.74E-15 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.30|P-value:9.09E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.64|P-value:2.11E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-6.31|P-value:1.01E-9 ID:SGTC_1721|Compound:st082152|FD-Score:3.53|P-value:3.14E-4 ID:SGTC_1722|Compound:st034304|FD-Score:4.05|P-value:4.59E-5 ID:SGTC_1723|Compound:st034307|FD-Score:3.69|P-value:1.77E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.00|P-value:6.90E-7 ID:SGTC_1765|Compound:st044815|FD-Score:3.26|P-value:7.91E-4 ID:SGTC_1843|Compound:st055809|FD-Score:-3.86|P-value:1.31E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.53|P-value:8.89E-6 ID:SGTC_1887|Compound:st057648|FD-Score:4.16|P-value:2.95E-5 ID:SGTC_1935|Compound:st074532|FD-Score:3.43|P-value:4.46E-4 ID:SGTC_2095|Compound:5432005|FD-Score:4.92|P-value:1.04E-6 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-4.33|P-value:2.05E-5 ID:SGTC_2283|Compound:7944793|FD-Score:3.60|P-value:2.49E-4 ID:SGTC_2423|Compound:st077119|FD-Score:3.79|P-value:1.25E-4 ID:SGTC_2431|Compound:5490079|FD-Score:4.50|P-value:7.08E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.26|P-value:2.73E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:-4.15|P-value:4.25E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.47|P-value:6.80E-8 ID:SGTC_2809|Compound:7964133|FD-Score:5.54|P-value:4.62E-8 ID:SGTC_2896|Compound:9052656|FD-Score:-5.91|P-value:9.76E-9 ID:SGTC_2993|Compound:9054257|FD-Score:-5.03|P-value:9.19E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.57|P-value:2.74E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-5.68|P-value:3.49E-8 ID:SGTC_3063|Compound:1327-0034|FD-Score:-3.57|P-value:3.70E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-5.23|P-value:3.55E-7 ID:SGTC_3193|Compound:9111931|FD-Score:5.54|P-value:4.76E-8 ID:SGTC_3197|Compound:9113331|FD-Score:-3.28|P-value:9.86E-4 ID:SGTC_3206|Compound:9114350|FD-Score:5.53|P-value:4.87E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-3.85|P-value:1.34E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-5.50|P-value:9.03E-8 ID:SGTC_56|Compound:0957-0365|FD-Score:5.62|P-value:2.98E-8 ID:SGTC_57|Compound:miconazole|FD-Score:3.32|P-value:6.40E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:8.93|P-value:4.76E-18 ID:SGTC_59|Compound:0330-0160|FD-Score:7.34|P-value:8.12E-13 ID:SGTC_62|Compound:0180-0423|FD-Score:3.39|P-value:5.17E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.76|P-value:3.22E-6 ID:SGTC_87|Compound:0100-0052|FD-Score:3.27|P-value:7.76E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:4.48|P-value:7.73E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.29|P-value:9.39E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:6.27|P-value:7.57E-10 ID:SGTC_176|Compound:1486-1328|FD-Score:3.25|P-value:8.05E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.65|P-value:2.56E-8 ID:SGTC_285|Compound:1486-1325|FD-Score:3.73|P-value:1.55E-4 ID:SGTC_296|Compound:1222-0039|FD-Score:3.29|P-value:7.13E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:4.35|P-value:1.32E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:4.05|P-value:4.44E-5 ID:SGTC_527|Compound:4476-4215|FD-Score:3.68|P-value:1.84E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.40|P-value:4.97E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.60|P-value:2.49E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:6.32|P-value:5.84E-10 ID:SGTC_829|Compound:1635-0120|FD-Score:-4.19|P-value:3.62E-5 ID:SGTC_830|Compound:4076-0286|FD-Score:6.11|P-value:2.01E-9 ID:SGTC_831|Compound:k061-1017|FD-Score:6.74|P-value:4.38E-11 ID:SGTC_832|Compound:4083-0019|FD-Score:8.15|P-value:2.27E-15 ID:SGTC_879|Compound:0831-1030|FD-Score:4.24|P-value:2.08E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:4.61|P-value:4.22E-6 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.31|P-value:1.60E-5 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-10.50|P-value:8.86E-24 ID:SGTC_1109|Compound:tnp00067|FD-Score:-4.28|P-value:2.57E-5 ID:SGTC_1111|Compound:tnp00062|FD-Score:-8.14|P-value:4.74E-15 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.30|P-value:9.09E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.64|P-value:2.11E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-6.31|P-value:1.01E-9 ID:SGTC_1721|Compound:st082152|FD-Score:3.53|P-value:3.14E-4 ID:SGTC_1722|Compound:st034304|FD-Score:4.05|P-value:4.59E-5 ID:SGTC_1723|Compound:st034307|FD-Score:3.69|P-value:1.77E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.00|P-value:6.90E-7 ID:SGTC_1765|Compound:st044815|FD-Score:3.26|P-value:7.91E-4 ID:SGTC_1843|Compound:st055809|FD-Score:-3.86|P-value:1.31E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.53|P-value:8.89E-6 ID:SGTC_1887|Compound:st057648|FD-Score:4.16|P-value:2.95E-5 ID:SGTC_1935|Compound:st074532|FD-Score:3.43|P-value:4.46E-4 ID:SGTC_2095|Compound:5432005|FD-Score:4.92|P-value:1.04E-6 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-4.33|P-value:2.05E-5 ID:SGTC_2283|Compound:7944793|FD-Score:3.60|P-value:2.49E-4 ID:SGTC_2423|Compound:st077119|FD-Score:3.79|P-value:1.25E-4 ID:SGTC_2431|Compound:5490079|FD-Score:4.50|P-value:7.08E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.26|P-value:2.73E-5 ID:SGTC_2586|Compound:curcumin|FD-Score:-4.15|P-value:4.25E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.47|P-value:6.80E-8 ID:SGTC_2809|Compound:7964133|FD-Score:5.54|P-value:4.62E-8 ID:SGTC_2896|Compound:9052656|FD-Score:-5.91|P-value:9.76E-9 ID:SGTC_2993|Compound:9054257|FD-Score:-5.03|P-value:9.19E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.57|P-value:2.74E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-5.68|P-value:3.49E-8 ID:SGTC_3063|Compound:1327-0034|FD-Score:-3.57|P-value:3.70E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-5.23|P-value:3.55E-7 ID:SGTC_3193|Compound:9111931|FD-Score:5.54|P-value:4.76E-8 ID:SGTC_3197|Compound:9113331|FD-Score:-3.28|P-value:9.86E-4 ID:SGTC_3206|Compound:9114350|FD-Score:5.53|P-value:4.87E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-3.85|P-value:1.34E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-5.50|P-value:9.03E-8

Top fitness defect scores for YGL246C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 8.93 9.0 µM 4.76E-18 cell wall 101 2.19E-19
2 SGTC_832 4083-0019 8.15 164.0 µM 2.27E-15 translation 7 1.78E-16
3 SGTC_59 0330-0160 7.34 1.3 µM 8.12E-13 19 1.05E-13
4 SGTC_831 k061-1017 6.74 217.0 µM 4.38E-11 translation 7 7.97E-12
5 SGTC_800 3448-7362 6.32 155.0 µM 5.84E-10 translation 38 1.32E-10
6 SGTC_118 0083-0097 6.27 135.3 µM 7.57E-10 translation 15 1.75E-10
7 SGTC_830 4076-0286 6.11 57.8 µM 2.01E-9 translation 15 5.04E-10
8 SGTC_241 5hr heat shock (37°C) + cantharidin 5.65 155.4 µM 2.56E-8 cell wall signaling 37 7.94E-9
9 SGTC_56 0957-0365 5.62 83.2 µM 2.98E-8 Golgi 19 9.35E-9
10 SGTC_2809 7964133 5.54 13.0 µM 4.62E-8 60S ribosome export 9 1.50E-8
11 SGTC_3193 9111931 5.54 49.5 µM 4.76E-8 5 1.55E-8
12 SGTC_3206 9114350 5.53 49.5 µM 4.87E-8 3 1.59E-8
13 SGTC_2688 kanamycin b 5.47 96.7 µM 6.80E-8 calcium & mitochondrial duress 198 2.28E-8
14 SGTC_1750 st081859 5.00 2.4 µM 6.90E-7 endomembrane recycling 138 2.80E-7
15 SGTC_2095 5432005 4.92 200.0 µM 1.04E-6 4 4.35E-7
16 SGTC_1007 4101-0031 4.61 70.0 µM 4.22E-6 8 1.98E-6
17 SGTC_2431 5490079 4.50 200.0 µM 7.08E-6 19 3.46E-6
18 SGTC_103 0844-0104 4.48 51.8 µM 7.73E-6 ERAD & cell cycle 120 3.81E-6
19 SGTC_385 1494-0393 4.35 12.4 µM 1.32E-5 13 6.78E-6
20 SGTC_1024 4507-0395 4.31 26.0 µM 1.60E-5 TSC3-RPN4 19 8.34E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.272 9.27E-58 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.255 7.53E-51 YEL054C RPL12A hom Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.219 1.33E-37 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.216 1.36E-36 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.207 1.27E-33 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.190 1.40E-28 YML021C UNG1 hom Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
0.188 7.86E-28 YLR407W_p YLR407W_p hom Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
0.182 2.58E-26 YBR267W REI1 hom Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.180 1.28E-25 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.179 1.57E-25 YGR271C-A EFG1 hom Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.178 3.07E-25 YCR085W_d YCR085W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.177 8.08E-25 YML061C PIF1 hom DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
0.175 2.50E-24 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.173 6.03E-24 YGL135W RPL1B hom Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
0.169 1.10E-22 YGR165W MRPS35 hom Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets