YGL253W / HXK2

Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL253W / HXK2

Click on Significant Values for Screen Details ID:SGTC_257|Compound:daunorubicin|FD-Score:3.71|P-value:1.24E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.23|P-value:1.54E-5 ID:SGTC_297|Compound:0986-0032|FD-Score:-3.52|P-value:2.65E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.82|P-value:1.02E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:4.91|P-value:6.39E-7 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:4.19|P-value:1.78E-5 ID:SGTC_398|Compound:bronopol|FD-Score:-3.39|P-value:4.19E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.64|P-value:1.64E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.18|P-value:8.46E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:3.73|P-value:1.19E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:3.52|P-value:2.63E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:-3.91|P-value:5.75E-5 ID:SGTC_603|Compound:3794-0023|FD-Score:3.19|P-value:8.15E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:4.55|P-value:3.55E-6 ID:SGTC_664|Compound:1222-0054|FD-Score:6.19|P-value:5.02E-10 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.15|P-value:9.30E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:4.40|P-value:7.00E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:3.16|P-value:9.16E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:3.53|P-value:2.53E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.14|P-value:9.64E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.15|P-value:9.50E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:6.45|P-value:9.96E-11 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.19|P-value:8.36E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:-3.22|P-value:7.63E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:-4.42|P-value:6.70E-6 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.33|P-value:5.16E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.19|P-value:8.25E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.26|P-value:6.44E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:9.15|P-value:9.28E-20 ID:SGTC_1629|Compound:st005085|FD-Score:-3.57|P-value:2.14E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.24|P-value:6.98E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.23|P-value:7.25E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.59|P-value:2.00E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.41|P-value:3.82E-4 ID:SGTC_2006|Compound:4011487|FD-Score:5.25|P-value:1.15E-7 ID:SGTC_2031|Compound:5180455|FD-Score:4.74|P-value:1.47E-6 ID:SGTC_2036|Compound:5160441|FD-Score:5.91|P-value:2.83E-9 ID:SGTC_2060|Compound:5238662|FD-Score:-3.23|P-value:7.34E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.45|P-value:3.32E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.50|P-value:2.81E-4 ID:SGTC_2279|Compound:7948423|FD-Score:3.22|P-value:7.50E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.38|P-value:7.95E-6 ID:SGTC_2322|Compound:7413319|FD-Score:6.19|P-value:5.26E-10 ID:SGTC_2326|Compound:6164455|FD-Score:3.54|P-value:2.40E-4 ID:SGTC_2333|Compound:9017922|FD-Score:3.65|P-value:1.57E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.34|P-value:4.90E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:6.03|P-value:1.37E-9 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.21|P-value:1.66E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.22|P-value:7.57E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.28|P-value:6.06E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.08|P-value:2.88E-5 ID:SGTC_2830|Compound:7992007|FD-Score:3.79|P-value:9.25E-5 ID:SGTC_2833|Compound:9001315|FD-Score:3.83|P-value:7.96E-5 ID:SGTC_2858|Compound:9025781|FD-Score:7.94|P-value:2.51E-15 ID:SGTC_2896|Compound:9052656|FD-Score:3.35|P-value:4.75E-4 ID:SGTC_2920|Compound:7982904|FD-Score:-7.55|P-value:4.88E-14 ID:SGTC_2921|Compound:7974240|FD-Score:-6.75|P-value:1.40E-11 ID:SGTC_2926|Compound:9010726|FD-Score:-4.30|P-value:1.14E-5 ID:SGTC_2955|Compound:9076254|FD-Score:-4.39|P-value:7.68E-6 ID:SGTC_3201|Compound:9113999|FD-Score:4.03|P-value:3.46E-5 ID:SGTC_3206|Compound:9114350|FD-Score:4.14|P-value:2.22E-5 ID:SGTC_3211|Compound:9128781|FD-Score:6.79|P-value:1.10E-11 ID:SGTC_3256|Compound:9138902|FD-Score:3.18|P-value:8.64E-4 ID:SGTC_3282|Compound:9108498|FD-Score:-3.37|P-value:4.49E-4 ID:SGTC_3296|Compound:9122258|FD-Score:4.28|P-value:1.20E-5 ID:SGTC_3298|Compound:9116858|FD-Score:3.56|P-value:2.19E-4 ID:SGTC_3333|Compound:9141820|FD-Score:-4.22|P-value:1.59E-5 ID:SGTC_257|Compound:daunorubicin|FD-Score:3.71|P-value:1.24E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.23|P-value:1.54E-5 ID:SGTC_297|Compound:0986-0032|FD-Score:-3.52|P-value:2.65E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.82|P-value:1.02E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:4.91|P-value:6.39E-7 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:4.19|P-value:1.78E-5 ID:SGTC_398|Compound:bronopol|FD-Score:-3.39|P-value:4.19E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:3.64|P-value:1.64E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.18|P-value:8.46E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:3.73|P-value:1.19E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:3.52|P-value:2.63E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:-3.91|P-value:5.75E-5 ID:SGTC_603|Compound:3794-0023|FD-Score:3.19|P-value:8.15E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:4.55|P-value:3.55E-6 ID:SGTC_664|Compound:1222-0054|FD-Score:6.19|P-value:5.02E-10 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.15|P-value:9.30E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:4.40|P-value:7.00E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:3.16|P-value:9.16E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:3.53|P-value:2.53E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:3.14|P-value:9.64E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.15|P-value:9.50E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:6.45|P-value:9.96E-11 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.19|P-value:8.36E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:-3.22|P-value:7.63E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:-4.42|P-value:6.70E-6 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.33|P-value:5.16E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.19|P-value:8.25E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.26|P-value:6.44E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:9.15|P-value:9.28E-20 ID:SGTC_1629|Compound:st005085|FD-Score:-3.57|P-value:2.14E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.24|P-value:6.98E-4 ID:SGTC_1712|Compound:st032288|FD-Score:-3.23|P-value:7.25E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.59|P-value:2.00E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.41|P-value:3.82E-4 ID:SGTC_2006|Compound:4011487|FD-Score:5.25|P-value:1.15E-7 ID:SGTC_2031|Compound:5180455|FD-Score:4.74|P-value:1.47E-6 ID:SGTC_2036|Compound:5160441|FD-Score:5.91|P-value:2.83E-9 ID:SGTC_2060|Compound:5238662|FD-Score:-3.23|P-value:7.34E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.45|P-value:3.32E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.50|P-value:2.81E-4 ID:SGTC_2279|Compound:7948423|FD-Score:3.22|P-value:7.50E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.38|P-value:7.95E-6 ID:SGTC_2322|Compound:7413319|FD-Score:6.19|P-value:5.26E-10 ID:SGTC_2326|Compound:6164455|FD-Score:3.54|P-value:2.40E-4 ID:SGTC_2333|Compound:9017922|FD-Score:3.65|P-value:1.57E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.34|P-value:4.90E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:6.03|P-value:1.37E-9 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.21|P-value:1.66E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.22|P-value:7.57E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.28|P-value:6.06E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.08|P-value:2.88E-5 ID:SGTC_2830|Compound:7992007|FD-Score:3.79|P-value:9.25E-5 ID:SGTC_2833|Compound:9001315|FD-Score:3.83|P-value:7.96E-5 ID:SGTC_2858|Compound:9025781|FD-Score:7.94|P-value:2.51E-15 ID:SGTC_2896|Compound:9052656|FD-Score:3.35|P-value:4.75E-4 ID:SGTC_2920|Compound:7982904|FD-Score:-7.55|P-value:4.88E-14 ID:SGTC_2921|Compound:7974240|FD-Score:-6.75|P-value:1.40E-11 ID:SGTC_2926|Compound:9010726|FD-Score:-4.30|P-value:1.14E-5 ID:SGTC_2955|Compound:9076254|FD-Score:-4.39|P-value:7.68E-6 ID:SGTC_3201|Compound:9113999|FD-Score:4.03|P-value:3.46E-5 ID:SGTC_3206|Compound:9114350|FD-Score:4.14|P-value:2.22E-5 ID:SGTC_3211|Compound:9128781|FD-Score:6.79|P-value:1.10E-11 ID:SGTC_3256|Compound:9138902|FD-Score:3.18|P-value:8.64E-4 ID:SGTC_3282|Compound:9108498|FD-Score:-3.37|P-value:4.49E-4 ID:SGTC_3296|Compound:9122258|FD-Score:4.28|P-value:1.20E-5 ID:SGTC_3298|Compound:9116858|FD-Score:3.56|P-value:2.19E-4 ID:SGTC_3333|Compound:9141820|FD-Score:-4.22|P-value:1.59E-5

Top fitness defect scores for YGL253W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1287 0971-0001 9.15 34.6 µM 9.28E-20 sphingolipid biosynthesis & PDR1 13 2.89E-20
2 SGTC_2858 9025781 7.94 9.0 µM 2.51E-15 sphingolipid biosynthesis & PDR1 27 1.04E-15
3 SGTC_3211 9128781 6.79 49.5 µM 1.10E-11 15 5.79E-12
4 SGTC_955 1216-0110 6.45 33.0 µM 9.96E-11 sphingolipid biosynthesis & PDR1 29 5.56E-11
5 SGTC_664 1222-0054 6.19 14.9 µM 5.02E-10 sphingolipid biosynthesis & PDR1 14 2.93E-10
6 SGTC_2322 7413319 6.19 38.2 µM 5.26E-10 ERAD & cell cycle 62 3.08E-10
7 SGTC_2568 diosmetin 6.03 100.0 µM 1.37E-9 3 8.24E-10
8 SGTC_2036 5160441 5.91 105.0 µM 2.83E-9 NEO1 44 1.73E-9
9 SGTC_2006 4011487 5.25 200.0 µM 1.15E-7 endomembrane recycling 57 7.78E-8
10 SGTC_352 k048-0007 4.91 5.1 µM 6.39E-7 ERAD & cell cycle 51 4.55E-7
11 SGTC_2031 5180455 4.74 200.0 µM 1.47E-6 7 1.07E-6
12 SGTC_628 1636-0253 4.55 16.0 µM 3.55E-6 sphingolipid biosynthesis & PDR1 22 2.65E-6
13 SGTC_683 panaxydol 4.40 45.0 µM 7.00E-6 sphingolipid biosynthesis & PDR1 24 5.31E-6
14 SGTC_2316 5844804 4.38 197.4 µM 7.95E-6 20 6.06E-6
15 SGTC_3296 9122258 4.28 64.0 µM 1.20E-5 redox potentiating 19 9.25E-6
16 SGTC_266 formosanin C 4.23 406.0 nM 1.54E-5 sphingolipid biosynthesis & PDR1 58 1.19E-5
17 SGTC_2573 2',4'-dihydroxychalcone 4.21 15.0 µM 1.66E-5 40 1.29E-5
18 SGTC_387 norethindrone acetate 4.19 66.7 µM 1.78E-5 10 1.39E-5
19 SGTC_3206 9114350 4.14 49.5 µM 2.22E-5 14 1.74E-5
20 SGTC_3201 9113999 4.03 49.5 µM 3.46E-5 34 2.74E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.287 1.85E-64 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.271 2.27E-57 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.248 4.74E-48 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.239 5.40E-45 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.226 3.63E-40 YHR112C YHR112C hom Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
0.222 1.04E-38 YDR072C IPT1 hom Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
0.206 1.78E-33 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.191 8.09E-29 YGR164W_d YGR164W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.179 1.46E-25 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.179 1.72E-25 YDR326C YSP2 hom Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.175 1.61E-24 YDR028C REG1 hom Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
0.175 2.18E-24 YBR036C CSG2 hom Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
0.170 2.93E-23 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.167 2.47E-22 YDR073W SNF11 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
0.167 2.41E-22 YGL013C PDR1 hom Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication