YGR058W / PEF1

Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR058W / PEF1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:19.50|P-value:1.42E-96 ID:SGTC_66|Compound:0109-0187|FD-Score:-4.57|P-value:4.93E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.86|P-value:1.84E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.58|P-value:6.44E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:6.70|P-value:3.95E-13 ID:SGTC_138|Compound:0850-0259|FD-Score:4.23|P-value:3.12E-6 ID:SGTC_307|Compound:1501-1223|FD-Score:3.13|P-value:4.16E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:3.77|P-value:2.79E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.02|P-value:6.08E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.09|P-value:4.62E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:10.90|P-value:1.50E-31 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.10|P-value:4.55E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:5.15|P-value:1.84E-8 ID:SGTC_638|Compound:3970-0837|FD-Score:3.47|P-value:1.06E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:2.96|P-value:7.89E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.12|P-value:4.22E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.83|P-value:2.07E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:-5.05|P-value:3.29E-8 ID:SGTC_1129|Compound:3453-1384|FD-Score:-5.76|P-value:3.60E-10 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.12|P-value:5.25E-6 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:4.94|P-value:6.56E-8 ID:SGTC_1563|Compound:conessine|FD-Score:4.25|P-value:2.81E-6 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.50|P-value:9.35E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.44|P-value:1.17E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.48|P-value:1.00E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:4.83|P-value:1.20E-7 ID:SGTC_1610|Compound:st001799|FD-Score:-2.94|P-value:8.14E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.43|P-value:1.12E-6 ID:SGTC_1719|Compound:st032227|FD-Score:3.94|P-value:1.25E-5 ID:SGTC_1837|Compound:st055472|FD-Score:-2.91|P-value:9.17E-4 ID:SGTC_2049|Compound:5262831|FD-Score:2.90|P-value:9.63E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.00|P-value:6.66E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.04|P-value:5.83E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.75|P-value:3.13E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:3.90|P-value:1.56E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.70|P-value:3.89E-13 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.91|P-value:1.43E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-3.44|P-value:1.14E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.26|P-value:2.48E-4 ID:SGTC_3082|Compound:9117008|FD-Score:-3.20|P-value:3.09E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.98|P-value:8.09E-11 ID:SGTC_3323|Compound:9138564|FD-Score:3.22|P-value:2.91E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-4.98|P-value:4.98E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:19.50|P-value:1.42E-96 ID:SGTC_66|Compound:0109-0187|FD-Score:-4.57|P-value:4.93E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.86|P-value:1.84E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.58|P-value:6.44E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:6.70|P-value:3.95E-13 ID:SGTC_138|Compound:0850-0259|FD-Score:4.23|P-value:3.12E-6 ID:SGTC_307|Compound:1501-1223|FD-Score:3.13|P-value:4.16E-4 ID:SGTC_326|Compound:k292-0812|FD-Score:3.77|P-value:2.79E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.02|P-value:6.08E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:-3.09|P-value:4.62E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:10.90|P-value:1.50E-31 ID:SGTC_517|Compound:1365-0151|FD-Score:-3.10|P-value:4.55E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:5.15|P-value:1.84E-8 ID:SGTC_638|Compound:3970-0837|FD-Score:3.47|P-value:1.06E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:2.96|P-value:7.89E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.12|P-value:4.22E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.83|P-value:2.07E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:-5.05|P-value:3.29E-8 ID:SGTC_1129|Compound:3453-1384|FD-Score:-5.76|P-value:3.60E-10 ID:SGTC_1131|Compound:3454-3128|FD-Score:4.12|P-value:5.25E-6 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:4.94|P-value:6.56E-8 ID:SGTC_1563|Compound:conessine|FD-Score:4.25|P-value:2.81E-6 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.50|P-value:9.35E-5 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.44|P-value:1.17E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.48|P-value:1.00E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:4.83|P-value:1.20E-7 ID:SGTC_1610|Compound:st001799|FD-Score:-2.94|P-value:8.14E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.43|P-value:1.12E-6 ID:SGTC_1719|Compound:st032227|FD-Score:3.94|P-value:1.25E-5 ID:SGTC_1837|Compound:st055472|FD-Score:-2.91|P-value:9.17E-4 ID:SGTC_2049|Compound:5262831|FD-Score:2.90|P-value:9.63E-4 ID:SGTC_2359|Compound:9039622|FD-Score:3.00|P-value:6.66E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.04|P-value:5.83E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.75|P-value:3.13E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:3.90|P-value:1.56E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.70|P-value:3.89E-13 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.91|P-value:1.43E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-3.44|P-value:1.14E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.26|P-value:2.48E-4 ID:SGTC_3082|Compound:9117008|FD-Score:-3.20|P-value:3.09E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.98|P-value:8.09E-11 ID:SGTC_3323|Compound:9138564|FD-Score:3.22|P-value:2.91E-4 ID:SGTC_3330|Compound:9141424|FD-Score:-4.98|P-value:4.98E-8

Top fitness defect scores for YGR058W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 19.50 9.0 µM 1.42E-96 cell wall 11 9.24E-85
2 SGTC_457 grayanotoxin iii 10.90 135.0 µM 1.50E-31 6 7.47E-28
3 SGTC_2737 bleomycin 6.70 30.5 nM 3.89E-13 Golgi 28 1.02E-11
4 SGTC_123 0083-0120 6.70 66.7 µM 3.95E-13 15 1.03E-11
5 SGTC_3214 9129694 5.98 49.5 µM 8.09E-11 4 1.10E-9
6 SGTC_586 0957-0265 5.15 8.2 µM 1.84E-8 cell wall 26 1.29E-7
7 SGTC_1546 5-methoxyflavanone 4.94 78.7 µM 6.56E-8 7 3.95E-7
8 SGTC_1572 epiandrosterone 4.83 68.9 µM 1.20E-7 17 6.69E-7
9 SGTC_1687 st020778 4.43 55.4 µM 1.12E-6 155 4.78E-6
10 SGTC_1563 conessine 4.25 56.1 µM 2.81E-6 10 1.08E-5
11 SGTC_138 0850-0259 4.23 40.9 µM 3.12E-6 cell wall 180 1.18E-5
12 SGTC_1131 3454-3128 4.12 32.3 µM 5.25E-6 38 1.86E-5
13 SGTC_1719 st032227 3.94 49.2 µM 1.25E-5 23 4.00E-5
14 SGTC_2736 butenafine 3.90 14.7 µM 1.56E-5 31 4.85E-5
15 SGTC_70 0141-0289 3.86 43.3 µM 1.84E-5 mitochondrial stress 182 5.62E-5
16 SGTC_326 k292-0812 3.77 17.5 µM 2.79E-5 9 8.12E-5
17 SGTC_2722 naftopidil 3.75 47.2 µM 3.13E-5 10 8.98E-5
18 SGTC_122 0236-0016 3.58 402.4 µM 6.44E-5 RNA processing & uracil transport 49 1.70E-4
19 SGTC_1564 D-Arginine 3.50 115.0 µM 9.35E-5 45 2.36E-4
20 SGTC_1569 carminic acid 3.48 40.6 µM 1.00E-4 34 2.51E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.425 5.09E-147 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.384 3.84E-118 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.382 4.55E-117 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.365 1.91E-106 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.352 3.09E-98 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.351 1.12E-97 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.330 5.05E-86 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.328 3.47E-85 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.324 5.05E-83 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.312 1.32E-76 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.307 3.04E-74 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.304 1.06E-72 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.303 3.83E-72 YNL211C_p YNL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
0.297 2.35E-69 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.296 1.03E-68 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication