YGR060W / ERG25

C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR060W / ERG25

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:5.22|P-value:4.20E-6 ID:SGTC_23|Compound:0213-0011|FD-Score:5.67|P-value:6.34E-7 ID:SGTC_43|Compound:0570-0105|FD-Score:4.06|P-value:2.71E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-6.88|P-value:1.70E-9 ID:SGTC_64|Compound:000l-0018|FD-Score:-4.62|P-value:3.45E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:-7.24|P-value:2.50E-10 ID:SGTC_142|Compound:0335-0849|FD-Score:-5.04|P-value:7.14E-6 ID:SGTC_147|Compound:4466-0538|FD-Score:-4.74|P-value:2.24E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-4.28|P-value:1.15E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:4.21|P-value:1.64E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:-3.65|P-value:8.33E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:8.08|P-value:2.54E-12 ID:SGTC_207|Compound:4185-0010|FD-Score:7.18|P-value:4.36E-10 ID:SGTC_208|Compound:k072-0098|FD-Score:4.30|P-value:1.23E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.10|P-value:2.36E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-3.71|P-value:7.00E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.72|P-value:7.74E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:-4.17|P-value:1.64E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.41|P-value:1.62E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:9.31|P-value:8.47E-16 ID:SGTC_374|Compound:0880-0231|FD-Score:11.60|P-value:1.60E-23 ID:SGTC_384|Compound:k007-0144|FD-Score:7.15|P-value:5.13E-10 ID:SGTC_515|Compound:4092-1064|FD-Score:4.66|P-value:3.46E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:17.50|P-value:4.14E-51 ID:SGTC_637|Compound:1181-0519|FD-Score:21.00|P-value:2.89E-72 ID:SGTC_649|Compound:1123-0311|FD-Score:8.86|P-value:1.85E-14 ID:SGTC_689|Compound:0833-0039|FD-Score:-4.23|P-value:1.33E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:13.20|P-value:6.40E-30 ID:SGTC_813|Compound:k007-0158|FD-Score:4.57|P-value:4.89E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:10.10|P-value:2.19E-18 ID:SGTC_868|Compound:0527-0199|FD-Score:4.21|P-value:1.67E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:6.22|P-value:5.36E-8 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.79|P-value:5.49E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-5.24|P-value:3.29E-6 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.81|P-value:5.13E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.80|P-value:5.37E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:-3.63|P-value:8.71E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.86|P-value:5.09E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.94|P-value:3.48E-4 ID:SGTC_1191|Compound:1487-1166|FD-Score:-5.41|P-value:1.62E-6 ID:SGTC_1230|Compound:0170-0365|FD-Score:7.90|P-value:7.25E-12 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.96|P-value:1.18E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.81|P-value:5.80E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.85|P-value:4.55E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.90|P-value:3.86E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.41|P-value:8.33E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.49|P-value:1.38E-6 ID:SGTC_1754|Compound:st042065|FD-Score:10.00|P-value:4.99E-18 ID:SGTC_2006|Compound:4011487|FD-Score:5.14|P-value:5.82E-6 ID:SGTC_2257|Compound:7960413|FD-Score:8.34|P-value:5.09E-13 ID:SGTC_2413|Compound:inermin|FD-Score:4.24|P-value:1.48E-4 ID:SGTC_2414|Compound:st077232|FD-Score:4.10|P-value:2.35E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.86|P-value:5.03E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.55|P-value:5.25E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.92|P-value:3.66E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-5.82|P-value:2.78E-7 ID:SGTC_2874|Compound:9043904|FD-Score:3.72|P-value:7.69E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.72|P-value:7.53E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:5.22|P-value:4.20E-6 ID:SGTC_23|Compound:0213-0011|FD-Score:5.67|P-value:6.34E-7 ID:SGTC_43|Compound:0570-0105|FD-Score:4.06|P-value:2.71E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-6.88|P-value:1.70E-9 ID:SGTC_64|Compound:000l-0018|FD-Score:-4.62|P-value:3.45E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:-7.24|P-value:2.50E-10 ID:SGTC_142|Compound:0335-0849|FD-Score:-5.04|P-value:7.14E-6 ID:SGTC_147|Compound:4466-0538|FD-Score:-4.74|P-value:2.24E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-4.28|P-value:1.15E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:4.21|P-value:1.64E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:-3.65|P-value:8.33E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:8.08|P-value:2.54E-12 ID:SGTC_207|Compound:4185-0010|FD-Score:7.18|P-value:4.36E-10 ID:SGTC_208|Compound:k072-0098|FD-Score:4.30|P-value:1.23E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.10|P-value:2.36E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-3.71|P-value:7.00E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.72|P-value:7.74E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:-4.17|P-value:1.64E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.41|P-value:1.62E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:9.31|P-value:8.47E-16 ID:SGTC_374|Compound:0880-0231|FD-Score:11.60|P-value:1.60E-23 ID:SGTC_384|Compound:k007-0144|FD-Score:7.15|P-value:5.13E-10 ID:SGTC_515|Compound:4092-1064|FD-Score:4.66|P-value:3.46E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:17.50|P-value:4.14E-51 ID:SGTC_637|Compound:1181-0519|FD-Score:21.00|P-value:2.89E-72 ID:SGTC_649|Compound:1123-0311|FD-Score:8.86|P-value:1.85E-14 ID:SGTC_689|Compound:0833-0039|FD-Score:-4.23|P-value:1.33E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:13.20|P-value:6.40E-30 ID:SGTC_813|Compound:k007-0158|FD-Score:4.57|P-value:4.89E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:10.10|P-value:2.19E-18 ID:SGTC_868|Compound:0527-0199|FD-Score:4.21|P-value:1.67E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:6.22|P-value:5.36E-8 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.79|P-value:5.49E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-5.24|P-value:3.29E-6 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.81|P-value:5.13E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.80|P-value:5.37E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:-3.63|P-value:8.71E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.86|P-value:5.09E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.94|P-value:3.48E-4 ID:SGTC_1191|Compound:1487-1166|FD-Score:-5.41|P-value:1.62E-6 ID:SGTC_1230|Compound:0170-0365|FD-Score:7.90|P-value:7.25E-12 ID:SGTC_1308|Compound:1187-1617|FD-Score:4.96|P-value:1.18E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.81|P-value:5.80E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-3.85|P-value:4.55E-4 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:-3.90|P-value:3.86E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.41|P-value:8.33E-5 ID:SGTC_1712|Compound:st032288|FD-Score:5.49|P-value:1.38E-6 ID:SGTC_1754|Compound:st042065|FD-Score:10.00|P-value:4.99E-18 ID:SGTC_2006|Compound:4011487|FD-Score:5.14|P-value:5.82E-6 ID:SGTC_2257|Compound:7960413|FD-Score:8.34|P-value:5.09E-13 ID:SGTC_2413|Compound:inermin|FD-Score:4.24|P-value:1.48E-4 ID:SGTC_2414|Compound:st077232|FD-Score:4.10|P-value:2.35E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.86|P-value:5.03E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.55|P-value:5.25E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.92|P-value:3.66E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-5.82|P-value:2.78E-7 ID:SGTC_2874|Compound:9043904|FD-Score:3.72|P-value:7.69E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.72|P-value:7.53E-4

Top fitness defect scores for YGR060W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_637 1181-0519 21.00 7.3 µM 2.89E-72 fatty acid desaturase (OLE1) 1 8.32E-98 8.69
2 SGTC_568 1193-0097 17.50 7.5 µM 4.14E-51 ERAD & cell cycle 2 5.42E-69 3.47
3 SGTC_768 0384-0033 13.20 77.3 µM 6.40E-30 fatty acid desaturase (OLE1) 2 3.84E-40 2.84
4 SGTC_374 0880-0231 11.60 82.3 µM 1.60E-23 iron homeostasis 1 1.95E-31 4.68
5 SGTC_834 0312-0008 10.10 75.7 µM 2.19E-18 NEO1 3 1.90E-24 0.38
6 SGTC_1754 st042065 10.00 16.1 µM 4.99E-18 NEO1-PIK1 5 5.81E-24 0.69
7 SGTC_352 k048-0007 9.31 5.1 µM 8.47E-16 ERAD & cell cycle 1 6.26E-21 2.89
8 SGTC_649 1123-0311 8.86 367.0 µM 1.85E-14 endomembrane recycling 3 4.14E-19 1.13
9 SGTC_2257 7960413 8.34 200.0 µM 5.09E-13 NEO1-PIK1 4 3.74E-17 0.34
10 SGTC_206 4546-0025 8.08 72.0 µM 2.54E-12 NEO1 3 3.32E-16 2.17
11 SGTC_1230 0170-0365 7.90 3.8 µM 7.25E-12 endomembrane recycling 8 1.38E-15 0.83
12 SGTC_207 4185-0010 7.18 4.5 µM 4.36E-10 endomembrane recycling 5 3.59E-13 0.55
13 SGTC_384 k007-0144 7.15 5.2 µM 5.13E-10 ERAD & cell cycle 1 4.48E-13 1.26
14 SGTC_875 0709-0210 6.22 25.7 µM 5.36E-8 fatty acid desaturase (OLE1) 4 2.45E-10 0.48
15 SGTC_23 0213-0011 5.67 40.0 µM 6.34E-7 copper-dependent oxidative stress 1 6.95E-9 1.05
16 SGTC_1712 st032288 5.49 53.4 µM 1.38E-6 calcium & mitochondrial duress 17 1.99E-8 0.02
17 SGTC_15 0266-0086 5.22 27.1 µM 4.20E-6 endomembrane recycling 8 8.98E-8 0.94
18 SGTC_2006 4011487 5.14 200.0 µM 5.82E-6 endomembrane recycling 11 1.39E-7 0.39
19 SGTC_1308 1187-1617 4.96 41.4 µM 1.18E-5 fatty acid desaturase (OLE1) 3 3.60E-7 0.25
20 SGTC_515 4092-1064 4.66 102.0 µM 3.46E-5 plasma membrane duress 12 1.55E-6 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.444 3.89E-162 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.376 3.82E-113 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.265 6.71E-55 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.264 1.93E-54 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.244 9.73E-47 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.242 6.76E-46 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.228 7.08E-41 YOR104W PIN2 hom Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated
0.225 1.18E-39 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.220 7.25E-38 YOL164W BDS1 hom Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources
0.219 8.57E-38 YOL144W NOP8 het Nucleolar protein required for 60S ribosomal subunit biogenesis
0.218 2.19E-37 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
0.214 4.87E-36 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.210 7.66E-35 YLR380W CSR1 hom Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
0.209 1.65E-34 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.206 1.95E-33 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton