YGR061C / ADE6

Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR061C / ADE6

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:4.47|P-value:1.77E-5 ID:SGTC_8|Compound:0354-0001|FD-Score:4.51|P-value:1.54E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.65|P-value:6.07E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:7.94|P-value:1.59E-13 ID:SGTC_65|Compound:0798-0905|FD-Score:3.59|P-value:4.28E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-7.36|P-value:2.00E-11 ID:SGTC_87|Compound:0100-0052|FD-Score:3.84|P-value:1.84E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:17.10|P-value:3.70E-55 ID:SGTC_163|Compound:1327-0036|FD-Score:3.84|P-value:1.87E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.19|P-value:5.30E-5 ID:SGTC_357|Compound:1325-0123|FD-Score:4.10|P-value:7.21E-5 ID:SGTC_443|Compound:clozapine|FD-Score:3.51|P-value:5.46E-4 ID:SGTC_448|Compound:monastrol|FD-Score:-4.57|P-value:2.34E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.96|P-value:1.19E-4 ID:SGTC_460|Compound:trequinsin|FD-Score:-3.77|P-value:4.14E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.22|P-value:4.70E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:3.41|P-value:7.63E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.37|P-value:8.70E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.37|P-value:8.67E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-4.03|P-value:1.73E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:6.33|P-value:2.94E-9 ID:SGTC_800|Compound:3448-7362|FD-Score:7.07|P-value:4.20E-11 ID:SGTC_853|Compound:0419-0306|FD-Score:3.47|P-value:6.26E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.06|P-value:8.39E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.97|P-value:2.06E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.80|P-value:3.74E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.41|P-value:7.51E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.72|P-value:4.77E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.72|P-value:2.72E-4 ID:SGTC_1809|Compound:st050081|FD-Score:-3.56|P-value:7.89E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.74|P-value:6.33E-8 ID:SGTC_2168|Compound:5727979|FD-Score:-4.66|P-value:1.62E-5 ID:SGTC_2239|Compound:6685329|FD-Score:-4.36|P-value:5.08E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.93|P-value:5.47E-6 ID:SGTC_2449|Compound:5812982|FD-Score:-3.71|P-value:4.94E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.69|P-value:3.05E-4 ID:SGTC_2483|Compound:5729050|FD-Score:4.29|P-value:3.61E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.40|P-value:3.14E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:-4.70|P-value:1.41E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.07|P-value:1.46E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:-3.78|P-value:3.89E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.62|P-value:3.84E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-5.30|P-value:1.09E-6 ID:SGTC_2823|Compound:7987624|FD-Score:-3.51|P-value:9.38E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.84|P-value:1.83E-4 ID:SGTC_3007|Compound:9078383|FD-Score:3.66|P-value:3.37E-4 ID:SGTC_3137|Compound:9097352|FD-Score:5.36|P-value:3.79E-7 ID:SGTC_3165|Compound:9101544|FD-Score:-3.52|P-value:9.00E-4 ID:SGTC_3244|Compound:9134489|FD-Score:14.20|P-value:1.22E-38 ID:SGTC_3288|Compound:9119690|FD-Score:-5.92|P-value:5.77E-8 ID:SGTC_7|Compound:0192-1582|FD-Score:4.47|P-value:1.77E-5 ID:SGTC_8|Compound:0354-0001|FD-Score:4.51|P-value:1.54E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.65|P-value:6.07E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:7.94|P-value:1.59E-13 ID:SGTC_65|Compound:0798-0905|FD-Score:3.59|P-value:4.28E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-7.36|P-value:2.00E-11 ID:SGTC_87|Compound:0100-0052|FD-Score:3.84|P-value:1.84E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:17.10|P-value:3.70E-55 ID:SGTC_163|Compound:1327-0036|FD-Score:3.84|P-value:1.87E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.19|P-value:5.30E-5 ID:SGTC_357|Compound:1325-0123|FD-Score:4.10|P-value:7.21E-5 ID:SGTC_443|Compound:clozapine|FD-Score:3.51|P-value:5.46E-4 ID:SGTC_448|Compound:monastrol|FD-Score:-4.57|P-value:2.34E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.96|P-value:1.19E-4 ID:SGTC_460|Compound:trequinsin|FD-Score:-3.77|P-value:4.14E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.22|P-value:4.70E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:3.41|P-value:7.63E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.37|P-value:8.70E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.37|P-value:8.67E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:-4.03|P-value:1.73E-4 ID:SGTC_791|Compound:1348-1292|FD-Score:6.33|P-value:2.94E-9 ID:SGTC_800|Compound:3448-7362|FD-Score:7.07|P-value:4.20E-11 ID:SGTC_853|Compound:0419-0306|FD-Score:3.47|P-value:6.26E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.06|P-value:8.39E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.97|P-value:2.06E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-3.80|P-value:3.74E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.41|P-value:7.51E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.72|P-value:4.77E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.72|P-value:2.72E-4 ID:SGTC_1809|Compound:st050081|FD-Score:-3.56|P-value:7.89E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.74|P-value:6.33E-8 ID:SGTC_2168|Compound:5727979|FD-Score:-4.66|P-value:1.62E-5 ID:SGTC_2239|Compound:6685329|FD-Score:-4.36|P-value:5.08E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.93|P-value:5.47E-6 ID:SGTC_2449|Compound:5812982|FD-Score:-3.71|P-value:4.94E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.69|P-value:3.05E-4 ID:SGTC_2483|Compound:5729050|FD-Score:4.29|P-value:3.61E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.40|P-value:3.14E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:-4.70|P-value:1.41E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:-4.07|P-value:1.46E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:-3.78|P-value:3.89E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.62|P-value:3.84E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-5.30|P-value:1.09E-6 ID:SGTC_2823|Compound:7987624|FD-Score:-3.51|P-value:9.38E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.84|P-value:1.83E-4 ID:SGTC_3007|Compound:9078383|FD-Score:3.66|P-value:3.37E-4 ID:SGTC_3137|Compound:9097352|FD-Score:5.36|P-value:3.79E-7 ID:SGTC_3165|Compound:9101544|FD-Score:-3.52|P-value:9.00E-4 ID:SGTC_3244|Compound:9134489|FD-Score:14.20|P-value:1.22E-38 ID:SGTC_3288|Compound:9119690|FD-Score:-5.92|P-value:5.77E-8

Top fitness defect scores for YGR061C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_123 0083-0120 17.10 66.7 µM 3.70E-55 2 7.70E-66
2 SGTC_3244 9134489 14.20 49.5 µM 1.22E-38 3 6.36E-46
3 SGTC_46 0329-0187a 7.94 2.2 µM 1.59E-13 16 9.92E-16
4 SGTC_800 3448-7362 7.07 155.0 µM 4.20E-11 translation 23 7.85E-13
5 SGTC_791 1348-1292 6.33 225.0 µM 2.94E-9 8 1.25E-10
6 SGTC_1877 wortmannin 5.74 25.0 µM 6.33E-8 50 4.84E-9
7 SGTC_2582 agaric acid 5.40 100.0 µM 3.14E-7 redox potentiating 198 3.25E-8
8 SGTC_3137 9097352 5.36 49.5 µM 3.79E-7 12 4.07E-8
9 SGTC_8 0354-0001 4.51 70.0 µM 1.54E-5 57 3.29E-6
10 SGTC_7 0192-1582 4.47 4.2 µM 1.77E-5 43 3.88E-6
11 SGTC_2483 5729050 4.29 27.2 µM 3.61E-5 17 9.02E-6
12 SGTC_625 k213-0058 4.22 121.0 µM 4.70E-5 translation 88 1.23E-5
13 SGTC_356 1634-0012 4.19 32.5 µM 5.30E-5 20 1.42E-5
14 SGTC_357 1325-0123 4.10 420.0 µM 7.21E-5 6 2.04E-5
15 SGTC_951 1171-0579 4.06 82.7 µM 8.39E-5 cell wall 76 2.44E-5
16 SGTC_457 grayanotoxin iii 3.96 135.0 µM 1.19E-4 64 3.68E-5
17 SGTC_2953 9074162 3.84 66.5 µM 1.83E-4 27 6.10E-5
18 SGTC_87 0100-0052 3.84 35.5 µM 1.84E-4 117 6.16E-5
19 SGTC_163 1327-0036 3.84 7.5 µM 1.87E-4 112 6.26E-5
20 SGTC_1255 0566-0045 3.72 221.0 µM 2.72E-4 excess fatty acid 38 9.77E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.466 1.71E-180 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.375 1.63E-112 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.321 4.43E-81 YDR408C ADE8 hom Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.317 4.93E-79 YLR058C SHM2 hom Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.274 6.93E-59 YMR300C ADE4 hom Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.182 1.74E-26 YMR120C ADE17 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.119 5.35E-12 YDL040C NAT1 hom Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
0.119 5.56E-12 YOR141C ARP8 hom Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity
0.116 1.34E-11 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.115 2.09E-11 YGR044C RME1 hom Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.113 5.36E-11 YDR067C_p OCA6_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
0.110 1.88E-10 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.107 5.27E-10 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.107 5.85E-10 YKR080W MTD1 hom NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
0.105 1.13E-9 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake