YGR083C / GCD2

Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR083C / GCD2

Click on Significant Values for Screen Details ID:SGTC_81|Compound:0411-0046|FD-Score:6.98|P-value:2.86E-9 ID:SGTC_87|Compound:0100-0052|FD-Score:18.30|P-value:3.87E-53 ID:SGTC_104|Compound:0074-0074|FD-Score:4.44|P-value:1.05E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.71|P-value:9.70E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:5.66|P-value:1.18E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:4.05|P-value:3.60E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.97|P-value:4.55E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.07|P-value:3.44E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:7.15|P-value:1.17E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:4.50|P-value:8.70E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:3.77|P-value:8.19E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:7.00|P-value:2.62E-9 ID:SGTC_274|Compound:sirtinol|FD-Score:4.89|P-value:2.21E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:3.76|P-value:8.59E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:5.26|P-value:5.68E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.72|P-value:9.41E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.76|P-value:8.29E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:14.60|P-value:2.81E-34 ID:SGTC_393|Compound:acivicin|FD-Score:-4.54|P-value:7.39E-5 ID:SGTC_476|Compound:mead acid|FD-Score:-3.83|P-value:6.80E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.78|P-value:7.88E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.23|P-value:2.02E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:7.57|P-value:1.33E-10 ID:SGTC_540|Compound:1187-0821|FD-Score:4.59|P-value:6.39E-5 ID:SGTC_541|Compound:4513-1321|FD-Score:4.14|P-value:2.78E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:6.21|P-value:1.08E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:5.71|P-value:9.47E-7 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.70|P-value:4.40E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:4.08|P-value:3.33E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:5.07|P-value:1.16E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:4.16|P-value:2.60E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.72|P-value:9.67E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:4.09|P-value:3.21E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:4.15|P-value:2.68E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.97|P-value:4.58E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.91|P-value:5.58E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.28|P-value:1.80E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:5.27|P-value:5.37E-6 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:4.78|P-value:3.26E-5 ID:SGTC_1766|Compound:st045414|FD-Score:4.19|P-value:2.33E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.86|P-value:2.46E-5 ID:SGTC_1863|Compound:st057641|FD-Score:4.17|P-value:2.54E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:14.10|P-value:3.54E-32 ID:SGTC_1891|Compound:st059073|FD-Score:4.13|P-value:2.86E-4 ID:SGTC_1904|Compound:st060225|FD-Score:5.20|P-value:7.09E-6 ID:SGTC_1906|Compound:st060206|FD-Score:-6.12|P-value:1.61E-7 ID:SGTC_1977|Compound:st070720|FD-Score:4.35|P-value:1.41E-4 ID:SGTC_2004|Compound:4023728|FD-Score:4.28|P-value:1.77E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.96|P-value:4.78E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-5.95|P-value:3.39E-7 ID:SGTC_2323|Compound:6148992|FD-Score:5.26|P-value:5.69E-6 ID:SGTC_2411|Compound:st077133|FD-Score:21.40|P-value:2.12E-71 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.04|P-value:3.69E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.81|P-value:2.96E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:13.00|P-value:1.06E-27 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.87|P-value:6.16E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.86|P-value:6.32E-4 ID:SGTC_2781|Compound:5567276|FD-Score:5.96|P-value:3.25E-7 ID:SGTC_2786|Compound:7743617|FD-Score:-3.90|P-value:5.53E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-5.17|P-value:7.66E-6 ID:SGTC_2995|Compound:9067767|FD-Score:-4.41|P-value:1.14E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-6.19|P-value:1.15E-7 ID:SGTC_3193|Compound:9111931|FD-Score:3.71|P-value:9.88E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:6.98|P-value:2.86E-9 ID:SGTC_87|Compound:0100-0052|FD-Score:18.30|P-value:3.87E-53 ID:SGTC_104|Compound:0074-0074|FD-Score:4.44|P-value:1.05E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:3.71|P-value:9.70E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:5.66|P-value:1.18E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:4.05|P-value:3.60E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.97|P-value:4.55E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.07|P-value:3.44E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:7.15|P-value:1.17E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:4.50|P-value:8.70E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:3.77|P-value:8.19E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:7.00|P-value:2.62E-9 ID:SGTC_274|Compound:sirtinol|FD-Score:4.89|P-value:2.21E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:3.76|P-value:8.59E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:5.26|P-value:5.68E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.72|P-value:9.41E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.76|P-value:8.29E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:14.60|P-value:2.81E-34 ID:SGTC_393|Compound:acivicin|FD-Score:-4.54|P-value:7.39E-5 ID:SGTC_476|Compound:mead acid|FD-Score:-3.83|P-value:6.80E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.78|P-value:7.88E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.23|P-value:2.02E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:7.57|P-value:1.33E-10 ID:SGTC_540|Compound:1187-0821|FD-Score:4.59|P-value:6.39E-5 ID:SGTC_541|Compound:4513-1321|FD-Score:4.14|P-value:2.78E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:6.21|P-value:1.08E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:5.71|P-value:9.47E-7 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.70|P-value:4.40E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:4.08|P-value:3.33E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:5.07|P-value:1.16E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:4.16|P-value:2.60E-4 ID:SGTC_982|Compound:1491-0518|FD-Score:3.72|P-value:9.67E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:4.09|P-value:3.21E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:4.15|P-value:2.68E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.97|P-value:4.58E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.91|P-value:5.58E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:4.28|P-value:1.80E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:5.27|P-value:5.37E-6 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:4.78|P-value:3.26E-5 ID:SGTC_1766|Compound:st045414|FD-Score:4.19|P-value:2.33E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.86|P-value:2.46E-5 ID:SGTC_1863|Compound:st057641|FD-Score:4.17|P-value:2.54E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:14.10|P-value:3.54E-32 ID:SGTC_1891|Compound:st059073|FD-Score:4.13|P-value:2.86E-4 ID:SGTC_1904|Compound:st060225|FD-Score:5.20|P-value:7.09E-6 ID:SGTC_1906|Compound:st060206|FD-Score:-6.12|P-value:1.61E-7 ID:SGTC_1977|Compound:st070720|FD-Score:4.35|P-value:1.41E-4 ID:SGTC_2004|Compound:4023728|FD-Score:4.28|P-value:1.77E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.96|P-value:4.78E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-5.95|P-value:3.39E-7 ID:SGTC_2323|Compound:6148992|FD-Score:5.26|P-value:5.69E-6 ID:SGTC_2411|Compound:st077133|FD-Score:21.40|P-value:2.12E-71 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.04|P-value:3.69E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.81|P-value:2.96E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:13.00|P-value:1.06E-27 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.87|P-value:6.16E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.86|P-value:6.32E-4 ID:SGTC_2781|Compound:5567276|FD-Score:5.96|P-value:3.25E-7 ID:SGTC_2786|Compound:7743617|FD-Score:-3.90|P-value:5.53E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-5.17|P-value:7.66E-6 ID:SGTC_2995|Compound:9067767|FD-Score:-4.41|P-value:1.14E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-6.19|P-value:1.15E-7 ID:SGTC_3193|Compound:9111931|FD-Score:3.71|P-value:9.88E-4

Top fitness defect scores for YGR083C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2411 st077133 21.40 39.9 µM 2.12E-71 RNA pol III & RNase P/MRP 3 1.82E-101 0.64
2 SGTC_87 0100-0052 18.30 35.5 µM 3.87E-53 2 2.65E-75 7.28
3 SGTC_392 N-ethylmaleimide 14.60 8.3 µM 2.81E-34 RNA pol III & RNase P/MRP 2 2.71E-48 5.03
4 SGTC_1872 amphotericin b 14.10 148.0 nM 3.54E-32 RNA pol III & RNase P/MRP 4 2.75E-45 2.19
5 SGTC_2612 dictamnine 13.00 15.5 µM 1.06E-27 RNA pol III & RNase P/MRP 7 6.96E-39 3.18
6 SGTC_539 4859-0462 7.57 2.5 µM 1.33E-10 RNA pol III & RNase P/MRP 6 1.89E-14 0.07
7 SGTC_153 4077-0274 7.15 519.7 µM 1.17E-9 1 4.19E-13 0.96
8 SGTC_177 1327-0068 7.00 8.4 µM 2.62E-9 6 1.32E-12 0.78
9 SGTC_81 0411-0046 6.98 10.0 µM 2.86E-9 15 1.50E-12 0.01
10 SGTC_567 1080-0542 6.21 68.4 µM 1.08E-7 1 2.60E-10 1.93
11 SGTC_2781 5567276 5.96 71.4 µM 3.25E-7 2 1.25E-9 0.64
12 SGTC_611 3453-1652 5.71 23.7 µM 9.47E-7 10 5.69E-9 0.55
13 SGTC_130 0136-0219 5.66 337.7 µM 1.18E-6 1 7.76E-9 0.66
14 SGTC_1568 digitonin 5.27 820.0 nM 5.37E-6 sphingolipid biosynthesis & PDR1 5 6.66E-8 0.17
15 SGTC_318 1488-0629 5.26 67.2 µM 5.68E-6 copper-dependent oxidative stress 1 7.21E-8 0.78
16 SGTC_2323 6148992 5.26 148.3 µM 5.69E-6 8 7.22E-8 0.56
17 SGTC_1904 st060225 5.20 78.0 µM 7.09E-6 1 9.87E-8 0.53
18 SGTC_690 glyoxal 5.07 20.3 µM 1.16E-5 17 1.99E-7 0.08
19 SGTC_274 sirtinol 4.89 40.1 µM 2.21E-5 10 4.93E-7 0.21
20 SGTC_1768 st044301 4.86 37.2 µM 2.46E-5 1 5.73E-7 0.30

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.515 1.08E-226 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.411 4.60E-137 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.406 5.17E-133 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.310 1.86E-75 YDR211W GCD6 het Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.244 1.44E-46 YBR167C POP7 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.242 7.95E-46 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.201 6.75E-32 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.195 4.85E-30 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.193 2.23E-29 YNL134C YNL134C hom Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.192 4.28E-29 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.185 2.87E-27 YDL150W RPC53 het RNA polymerase III subunit C53
0.175 2.15E-24 YLR243W GPN3 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.170 3.29E-23 YMR283C RIT1 hom 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.167 2.28E-22 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.162 4.40E-21 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway