YGR112W / SHY1

Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR112W / SHY1

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:-3.05|P-value:2.02E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-2.89|P-value:4.07E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.51|P-value:2.36E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.40|P-value:4.08E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:14.60|P-value:1.75E-63 ID:SGTC_266|Compound:formosanin C|FD-Score:3.00|P-value:2.85E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:2.70|P-value:9.71E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.43|P-value:4.12E-5 ID:SGTC_336|Compound:4111-0029|FD-Score:2.80|P-value:6.69E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:4.24|P-value:5.50E-7 ID:SGTC_439|Compound:amiodarone|FD-Score:2.82|P-value:6.09E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:2.85|P-value:5.37E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.92|P-value:3.39E-6 ID:SGTC_530|Compound:4358-1714|FD-Score:2.82|P-value:5.96E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:2.72|P-value:9.07E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.45|P-value:3.27E-5 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.40|P-value:4.82E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:-2.81|P-value:5.55E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:-3.65|P-value:1.17E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.78|P-value:6.36E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-2.86|P-value:4.57E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.31|P-value:7.07E-5 ID:SGTC_1635|Compound:st007035|FD-Score:8.36|P-value:3.01E-22 ID:SGTC_1704|Compound:st028805|FD-Score:2.76|P-value:7.70E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.07|P-value:2.13E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.34|P-value:6.38E-5 ID:SGTC_2116|Compound:5511452|FD-Score:2.70|P-value:9.68E-4 ID:SGTC_2126|Compound:5251174|FD-Score:4.00|P-value:2.16E-6 ID:SGTC_2296|Compound:7971656|FD-Score:-2.92|P-value:3.59E-4 ID:SGTC_2301|Compound:7769476|FD-Score:2.92|P-value:4.04E-4 ID:SGTC_2490|Compound:carminic acid|FD-Score:2.96|P-value:3.38E-4 ID:SGTC_2492|Compound:derrusnin|FD-Score:2.82|P-value:6.02E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:2.83|P-value:5.87E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:2.82|P-value:6.06E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:2.80|P-value:6.47E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-2.83|P-value:5.17E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.62|P-value:1.40E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:-4.31|P-value:3.00E-7 ID:SGTC_2832|Compound:7992762|FD-Score:-3.90|P-value:3.17E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-5.22|P-value:7.62E-10 ID:SGTC_2835|Compound:9006588|FD-Score:-4.65|P-value:3.77E-8 ID:SGTC_2836|Compound:9010338|FD-Score:2.83|P-value:5.76E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-5.75|P-value:1.49E-11 ID:SGTC_2857|Compound:9024691|FD-Score:2.97|P-value:3.20E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.41|P-value:4.40E-5 ID:SGTC_2952|Compound:9073731|FD-Score:3.47|P-value:3.34E-5 ID:SGTC_3026|Compound:9090633|FD-Score:-3.21|P-value:1.01E-4 ID:SGTC_3072|Compound:9116419|FD-Score:3.00|P-value:2.91E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.17|P-value:8.27E-7 ID:SGTC_3274|Compound:9138298|FD-Score:-3.11|P-value:1.54E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.16|P-value:1.41E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.05|P-value:2.02E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-2.89|P-value:4.07E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.51|P-value:2.36E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.40|P-value:4.08E-5 ID:SGTC_236|Compound:1509-0011|FD-Score:14.60|P-value:1.75E-63 ID:SGTC_266|Compound:formosanin C|FD-Score:3.00|P-value:2.85E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:2.70|P-value:9.71E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.43|P-value:4.12E-5 ID:SGTC_336|Compound:4111-0029|FD-Score:2.80|P-value:6.69E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:4.24|P-value:5.50E-7 ID:SGTC_439|Compound:amiodarone|FD-Score:2.82|P-value:6.09E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:2.85|P-value:5.37E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.92|P-value:3.39E-6 ID:SGTC_530|Compound:4358-1714|FD-Score:2.82|P-value:5.96E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:2.72|P-value:9.07E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.45|P-value:3.27E-5 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.40|P-value:4.82E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:-2.81|P-value:5.55E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:-3.65|P-value:1.17E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.78|P-value:6.36E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:-2.86|P-value:4.57E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.31|P-value:7.07E-5 ID:SGTC_1635|Compound:st007035|FD-Score:8.36|P-value:3.01E-22 ID:SGTC_1704|Compound:st028805|FD-Score:2.76|P-value:7.70E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.07|P-value:2.13E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.34|P-value:6.38E-5 ID:SGTC_2116|Compound:5511452|FD-Score:2.70|P-value:9.68E-4 ID:SGTC_2126|Compound:5251174|FD-Score:4.00|P-value:2.16E-6 ID:SGTC_2296|Compound:7971656|FD-Score:-2.92|P-value:3.59E-4 ID:SGTC_2301|Compound:7769476|FD-Score:2.92|P-value:4.04E-4 ID:SGTC_2490|Compound:carminic acid|FD-Score:2.96|P-value:3.38E-4 ID:SGTC_2492|Compound:derrusnin|FD-Score:2.82|P-value:6.02E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:2.83|P-value:5.87E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:2.82|P-value:6.06E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:2.80|P-value:6.47E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-2.83|P-value:5.17E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-3.62|P-value:1.40E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:-4.31|P-value:3.00E-7 ID:SGTC_2832|Compound:7992762|FD-Score:-3.90|P-value:3.17E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-5.22|P-value:7.62E-10 ID:SGTC_2835|Compound:9006588|FD-Score:-4.65|P-value:3.77E-8 ID:SGTC_2836|Compound:9010338|FD-Score:2.83|P-value:5.76E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-5.75|P-value:1.49E-11 ID:SGTC_2857|Compound:9024691|FD-Score:2.97|P-value:3.20E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.41|P-value:4.40E-5 ID:SGTC_2952|Compound:9073731|FD-Score:3.47|P-value:3.34E-5 ID:SGTC_3026|Compound:9090633|FD-Score:-3.21|P-value:1.01E-4 ID:SGTC_3072|Compound:9116419|FD-Score:3.00|P-value:2.91E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.17|P-value:8.27E-7 ID:SGTC_3274|Compound:9138298|FD-Score:-3.11|P-value:1.54E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.16|P-value:1.41E-4

Top fitness defect scores for YGR112W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 14.60 5.3 µM 1.75E-63 108 2.45E-48
2 SGTC_1635 st007035 8.36 42.4 µM 3.01E-22 94 3.05E-17
3 SGTC_386 1509-0011 4.24 3.1 µM 5.50E-7 131 1.12E-5
4 SGTC_3189 9110682 4.17 49.5 µM 8.27E-7 29 1.53E-5
5 SGTC_2126 5251174 4.00 33.9 µM 2.16E-6 32 3.18E-5
6 SGTC_507 nimesulide 3.92 162.0 µM 3.39E-6 17 4.49E-5
7 SGTC_2952 9073731 3.47 13.8 µM 3.34E-5 tubulin folding & SWR complex 63 2.58E-4
8 SGTC_310 1273-0058 3.43 1.1 µM 4.12E-5 mitochondrial stress 296 3.04E-4
9 SGTC_2949 9072542 3.41 2.2 µM 4.40E-5 TSC3-RPN4 60 3.20E-4
10 SGTC_1009 4106-0057 3.40 9.9 µM 4.82E-5 24 3.42E-4
11 SGTC_1976 st070609 3.34 63.5 µM 6.38E-5 29 4.25E-4
12 SGTC_1299 1079-1439 3.31 3.7 µM 7.07E-5 TSC3-RPN4 33 4.59E-4
13 SGTC_3281 9140613 3.16 49.5 µM 1.41E-4 RPP1 & pyrimidine depletion 46 7.82E-4
14 SGTC_1941 st076221 3.07 61.8 µM 2.13E-4 sphingolipid biosynthesis & PDR1 137 0.00107
15 SGTC_266 formosanin C 3.00 406.0 nM 2.85E-4 sphingolipid biosynthesis & PDR1 116 0.00134
16 SGTC_3072 9116419 3.00 49.5 µM 2.91E-4 ERG2 56 0.00136
17 SGTC_2857 9024691 2.97 58.4 µM 3.20E-4 77 0.00147
18 SGTC_2490 carminic acid 2.96 95.4 µM 3.38E-4 28 0.00153
19 SGTC_2301 7769476 2.92 200.0 µM 4.04E-4 58 0.00176
20 SGTC_502 l-744,832 2.85 89.3 µM 5.37E-4 calcium & mitochondrial duress 96 0.00218

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.625 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.607 0 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.583 5.73E-305 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.571 4.52E-290 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.565 1.59E-282 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.561 1.79E-277 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.557 1.05E-272 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.555 4.46E-270 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.544 3.97E-258 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.543 1.56E-256 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.541 2.61E-254 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.538 5.73E-251 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.535 3.41E-248 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.530 1.29E-242 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.526 3.52E-238 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies