HIPHOP chemogenomics database
YGR124W / ASN2 
Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YGR124W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.122 | 1.39E-12 | YKL074C | MUD2 | hom | Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 |
| 0.120 | 3.17E-12 | YOL079W_d | YOL079W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| 0.107 | 5.33E-10 | YLR417W | VPS36 | hom | Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome |
| 0.106 | 6.83E-10 | YDR156W | RPA14 | hom | RNA polymerase I subunit A14 |
| 0.106 | 7.47E-10 | YOL158C | ENB1 | hom | Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment |
| 0.106 | 8.58E-10 | YDR147W | EKI1 | hom | Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication |
| 0.105 | 1.07E-9 | YKL004W | AUR1 | het | Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance |
| 0.105 | 1.24E-9 | YGR206W | MVB12 | hom | ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin |
| 0.103 | 2.03E-9 | YKL052C | ASK1 | het | Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress |
| 0.094 | 5.06E-8 | YLL037W_d | YLL037W_d | het | Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor |
| 0.091 | 1.45E-7 | YPL079W | RPL21B | hom | Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication |
| 0.086 | 6.07E-7 | YDL201W | TRM8 | hom | Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p |
| 0.084 | 1.20E-6 | YNL236W | SIN4 | hom | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription |
| 0.084 | 1.28E-6 | YNL097C | PHO23 | hom | Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 |
| 0.083 | 1.29E-6 | YNL219C | ALG9 | hom | Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation |