YGR136W / LSB1

Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR136W / LSB1

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.21|P-value:1.06E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:-4.03|P-value:2.09E-6 ID:SGTC_264|Compound:promethazine|FD-Score:-4.25|P-value:6.20E-7 ID:SGTC_380|Compound:3970-0968|FD-Score:-2.79|P-value:7.51E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:2.88|P-value:4.30E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-2.98|P-value:3.48E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:2.85|P-value:4.81E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:2.68|P-value:9.66E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:-3.25|P-value:1.08E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:5.58|P-value:6.23E-11 ID:SGTC_1209|Compound:2323-2347|FD-Score:3.80|P-value:5.67E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:2.77|P-value:6.73E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-7.53|P-value:3.54E-18 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.13|P-value:1.22E-12 ID:SGTC_1515|Compound:3460-0031|FD-Score:-2.73|P-value:9.63E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-2.85|P-value:6.02E-4 ID:SGTC_1968|Compound:st069590|FD-Score:2.77|P-value:6.73E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-2.72|P-value:9.88E-4 ID:SGTC_2048|Compound:5246248|FD-Score:2.89|P-value:4.18E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-2.87|P-value:5.42E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-5.16|P-value:1.93E-9 ID:SGTC_2131|Compound:toluylene red|FD-Score:-5.94|P-value:5.63E-12 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-2.82|P-value:6.70E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.26|P-value:8.39E-5 ID:SGTC_2351|Compound:9026858|FD-Score:-3.27|P-value:9.81E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:2.73|P-value:7.96E-4 ID:SGTC_2715|Compound:st079500|FD-Score:-2.96|P-value:3.73E-4 ID:SGTC_2791|Compound:5663188|FD-Score:2.74|P-value:7.66E-4 ID:SGTC_2798|Compound:7813637|FD-Score:2.86|P-value:4.62E-4 ID:SGTC_2799|Compound:7812892|FD-Score:2.84|P-value:5.00E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.13|P-value:1.52E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.08|P-value:1.28E-6 ID:SGTC_2941|Compound:9056145|FD-Score:3.57|P-value:1.89E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.15|P-value:1.38E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.37|P-value:6.18E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.28|P-value:7.55E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:3.21|P-value:1.06E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:-4.03|P-value:2.09E-6 ID:SGTC_264|Compound:promethazine|FD-Score:-4.25|P-value:6.20E-7 ID:SGTC_380|Compound:3970-0968|FD-Score:-2.79|P-value:7.51E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:2.88|P-value:4.30E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:-2.98|P-value:3.48E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:2.85|P-value:4.81E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:2.68|P-value:9.66E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:-3.25|P-value:1.08E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:5.58|P-value:6.23E-11 ID:SGTC_1209|Compound:2323-2347|FD-Score:3.80|P-value:5.67E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:2.77|P-value:6.73E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-7.53|P-value:3.54E-18 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.13|P-value:1.22E-12 ID:SGTC_1515|Compound:3460-0031|FD-Score:-2.73|P-value:9.63E-4 ID:SGTC_1957|Compound:st077035|FD-Score:-2.85|P-value:6.02E-4 ID:SGTC_1968|Compound:st069590|FD-Score:2.77|P-value:6.73E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-2.72|P-value:9.88E-4 ID:SGTC_2048|Compound:5246248|FD-Score:2.89|P-value:4.18E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-2.87|P-value:5.42E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:-5.16|P-value:1.93E-9 ID:SGTC_2131|Compound:toluylene red|FD-Score:-5.94|P-value:5.63E-12 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-2.82|P-value:6.70E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.26|P-value:8.39E-5 ID:SGTC_2351|Compound:9026858|FD-Score:-3.27|P-value:9.81E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:2.73|P-value:7.96E-4 ID:SGTC_2715|Compound:st079500|FD-Score:-2.96|P-value:3.73E-4 ID:SGTC_2791|Compound:5663188|FD-Score:2.74|P-value:7.66E-4 ID:SGTC_2798|Compound:7813637|FD-Score:2.86|P-value:4.62E-4 ID:SGTC_2799|Compound:7812892|FD-Score:2.84|P-value:5.00E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.13|P-value:1.52E-4 ID:SGTC_2814|Compound:7977482|FD-Score:4.08|P-value:1.28E-6 ID:SGTC_2941|Compound:9056145|FD-Score:3.57|P-value:1.89E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.15|P-value:1.38E-4 ID:SGTC_3049|Compound:9092223|FD-Score:-3.37|P-value:6.18E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.28|P-value:7.55E-5

Top fitness defect scores for YGR136W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1181 1222-0449 5.58 220.0 µM 6.23E-11 8 1.17E-8
2 SGTC_2814 7977482 4.08 43.0 µM 1.28E-6 45 2.29E-5
3 SGTC_1209 2323-2347 3.80 50.3 µM 5.67E-6 20 7.14E-5
4 SGTC_2941 9056145 3.57 11.2 µM 1.89E-5 26 1.79E-4
5 SGTC_3063 1327-0034 3.28 4.0 µM 7.55E-5 61 5.19E-4
6 SGTC_2327 6189656 3.26 200.0 µM 8.39E-5 47 5.63E-4
7 SGTC_65 0798-0905 3.21 35.0 µM 1.06E-4 calcium & mitochondrial duress 141 6.73E-4
8 SGTC_2989 9073649 3.15 71.4 µM 1.38E-4 87 8.24E-4
9 SGTC_2807 7945238 3.13 52.0 µM 1.52E-4 79 8.88E-4
10 SGTC_2048 5246248 2.89 200.0 µM 4.18E-4 33 0.00193
11 SGTC_426 0988-0037 2.88 14.6 µM 4.30E-4 ERAD & cell cycle 94 0.00198
12 SGTC_2798 7813637 2.86 45.5 µM 4.62E-4 95 0.00209
13 SGTC_1111 tnp00062 2.85 510.7 nM 4.81E-4 49 0.00215
14 SGTC_2799 7812892 2.84 81.8 µM 5.00E-4 superoxide 89 0.00222
15 SGTC_1968 st069590 2.77 43.6 µM 6.73E-4 59 0.00279
16 SGTC_1226 3909-7979 2.77 35.4 µM 6.73E-4 102 0.00279
17 SGTC_2791 5663188 2.74 71.4 µM 7.66E-4 RPP1 & pyrimidine depletion 79 0.00308
18 SGTC_2563 tomatine 2.73 8.5 µM 7.96E-4 144 0.00318
19 SGTC_1139 k015-0024 2.68 46.9 µM 9.66E-4 PDR1 57 0.00369
20 SGTC_2724 maprotiline 2.66 69.6 µM 0.00105 ERG2 75 0.00393

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.363 1.25E-104 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.356 1.68E-100 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.352 3.91E-98 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.332 5.40E-87 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.326 2.42E-83 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.324 8.32E-83 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.321 2.57E-81 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.321 6.73E-81 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.316 1.03E-78 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.315 7.32E-78 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.308 1.45E-74 YDR517W GRH1 hom Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress
0.306 1.23E-73 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.299 9.96E-70 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.297 4.49E-69 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.291 1.09E-66 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth