HIPHOP chemogenomics database

YGR140W / CBF2

Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YGR140W / CBF2

Click on Significant Values for Screen Details

Top fitness defect scores for YGR140W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1723	st034307	6.63	2.5 µM	1.22E-13	mitochondrial processes	3	1.67E-11	0.34
2	SGTC_2158	5657123	6.49	3.6 µM	3.78E-13		2	4.24E-11	0.26
3	SGTC_1722	st034304	5.97	3.6 µM	2.18E-11	mitochondrial processes	2	1.19E-9	0.02
4	SGTC_1732	st034308	4.69	51.8 µM	1.14E-7		4	1.36E-6	0.30
5	SGTC_1204	4049-0315	4.25	18.3 µM	1.37E-6		8	1.06E-5	0.16
6	SGTC_3293	9120939	4.01	62.0 µM	4.88E-6		10	3.04E-5	0.40
7	SGTC_155	k072-0202	3.99	43.6 µM	5.42E-6		4	3.31E-5	0.20
8	SGTC_3292	9120825	3.73	59.8 µM	1.94E-5		3	9.51E-5	0.08
9	SGTC_2743	auranofin	3.71	4.3 µM	2.14E-5	RPP1 & pyrimidine depletion	13	1.03E-4	0.14
10	SGTC_1258	0671-0043	3.61	57.1 µM	3.41E-5		7	1.52E-4	0.22
11	SGTC_2014	4013930	3.57	200.0 µM	4.16E-5		6	1.79E-4	0.09
12	SGTC_478	N-arachidonylglycine	3.45	10.0 µM	7.17E-5		1	2.81E-4	0.61
13	SGTC_882	0843-0131	3.36	351.0 µM	1.06E-4		18	3.90E-4	0.08
14	SGTC_3320	9137005	3.26	35.1 µM	1.63E-4	endomembrane recycling	8	5.55E-4	0.08
15	SGTC_1445	4239-0156	3.14	97.7 µM	2.67E-4		8	8.36E-4	0.08
16	SGTC_2254	7938975	3.10	95.2 µM	3.15E-4		19	9.60E-4	0.12
17	SGTC_659	k018-0002	3.06	107.0 µM	3.75E-4		17	0.00111	0.04
18	SGTC_418	lividomycin a	3.04	100.0 µM	4.13E-4		9	0.00120	0.01
19	SGTC_473	2-arachidonoylglycerol	3.01	10.0 µM	4.48E-4		17	0.00129	0.01
20	SGTC_3342	9147791	3.01	14.5 µM	4.55E-4		9	0.00130	0.03

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.187	8.10E-28	YJR072C	NPA3	het	Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.174	3.47E-24	YOR159C	SME1	het	Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.172	7.80E-24	YLR117C	CLF1	het	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.161	6.25E-21	YML098W	TAF13	het	TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.146	2.25E-17	YER013W	PRP22	het	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.126	2.69E-13	YBL004W	UTP20	het	Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.124	5.93E-13	YAL034W-A	MTW1	het	Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
0.123	1.05E-12	YER159C	BUR6	het	Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
0.121	2.39E-12	YIL083C	CAB2	het	Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.117	1.09E-11	YFR031C	SMC2	het	Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus
0.117	1.33E-11	YDR088C	SLU7	het	RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.114	3.65E-11	YGL144C	ROG1	hom	Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication
0.114	4.03E-11	YHR166C	CDC23	het	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.108	4.20E-10	YGR280C	PXR1	het	Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
0.106	7.83E-10	YKL024C	URA6	het	Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)