YGR162W / TIF4631

Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR162W / TIF4631

Click on Significant Values for Screen Details ID:SGTC_104|Compound:0074-0074|FD-Score:4.32|P-value:3.63E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:4.96|P-value:2.73E-6 ID:SGTC_201|Compound:4262-0159|FD-Score:3.67|P-value:3.69E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.76|P-value:2.69E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.65|P-value:3.89E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:5.16|P-value:1.11E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.00|P-value:3.18E-6 ID:SGTC_582|Compound:0987-0078|FD-Score:4.07|P-value:9.12E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:7.62|P-value:1.55E-12 ID:SGTC_679|Compound:0422-0091|FD-Score:3.44|P-value:7.71E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.65|P-value:3.90E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.75|P-value:2.82E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.34|P-value:4.89E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:5.87|P-value:3.81E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:6.52|P-value:1.21E-9 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.56|P-value:5.22E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.24|P-value:4.85E-5 ID:SGTC_1423|Compound:4048-0112|FD-Score:3.45|P-value:7.47E-4 ID:SGTC_1728|Compound:st035614|FD-Score:4.79|P-value:5.51E-6 ID:SGTC_1781|Compound:st038725|FD-Score:6.70|P-value:4.34E-10 ID:SGTC_1813|Compound:st051246|FD-Score:-3.46|P-value:9.37E-4 ID:SGTC_1942|Compound:st076330|FD-Score:4.40|P-value:2.68E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.69|P-value:3.47E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:9.31|P-value:9.02E-18 ID:SGTC_2280|Compound:7785178|FD-Score:3.42|P-value:8.29E-4 ID:SGTC_2354|Compound:9060492|FD-Score:-3.68|P-value:4.71E-4 ID:SGTC_2355|Compound:9061122|FD-Score:-4.00|P-value:1.57E-4 ID:SGTC_2358|Compound:9037157|FD-Score:5.31|P-value:5.51E-7 ID:SGTC_2452|Compound:5863136|FD-Score:3.62|P-value:4.30E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.49|P-value:6.63E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:4.48|P-value:1.96E-5 ID:SGTC_2674|Compound:ligustilide|FD-Score:5.04|P-value:1.88E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.83|P-value:2.12E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.61|P-value:4.45E-4 ID:SGTC_2821|Compound:7996764|FD-Score:6.89|P-value:1.43E-10 ID:SGTC_2822|Compound:7994718|FD-Score:-4.24|P-value:6.62E-5 ID:SGTC_2823|Compound:7987624|FD-Score:-4.06|P-value:1.26E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.37|P-value:9.48E-4 ID:SGTC_3105|Compound:9121040|FD-Score:-3.68|P-value:4.67E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.43|P-value:7.92E-4 ID:SGTC_3120|Compound:9124077|FD-Score:4.30|P-value:3.85E-5 ID:SGTC_3133|Compound:9127736|FD-Score:4.43|P-value:2.37E-5 ID:SGTC_3210|Compound:9128157|FD-Score:3.64|P-value:4.01E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-8.31|P-value:2.57E-14 ID:SGTC_104|Compound:0074-0074|FD-Score:4.32|P-value:3.63E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:4.96|P-value:2.73E-6 ID:SGTC_201|Compound:4262-0159|FD-Score:3.67|P-value:3.69E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.76|P-value:2.69E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.65|P-value:3.89E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:5.16|P-value:1.11E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.00|P-value:3.18E-6 ID:SGTC_582|Compound:0987-0078|FD-Score:4.07|P-value:9.12E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:7.62|P-value:1.55E-12 ID:SGTC_679|Compound:0422-0091|FD-Score:3.44|P-value:7.71E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.65|P-value:3.90E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.75|P-value:2.82E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.34|P-value:4.89E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:5.87|P-value:3.81E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:6.52|P-value:1.21E-9 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.56|P-value:5.22E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.24|P-value:4.85E-5 ID:SGTC_1423|Compound:4048-0112|FD-Score:3.45|P-value:7.47E-4 ID:SGTC_1728|Compound:st035614|FD-Score:4.79|P-value:5.51E-6 ID:SGTC_1781|Compound:st038725|FD-Score:6.70|P-value:4.34E-10 ID:SGTC_1813|Compound:st051246|FD-Score:-3.46|P-value:9.37E-4 ID:SGTC_1942|Compound:st076330|FD-Score:4.40|P-value:2.68E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.69|P-value:3.47E-4 ID:SGTC_2129|Compound:girgensonine|FD-Score:9.31|P-value:9.02E-18 ID:SGTC_2280|Compound:7785178|FD-Score:3.42|P-value:8.29E-4 ID:SGTC_2354|Compound:9060492|FD-Score:-3.68|P-value:4.71E-4 ID:SGTC_2355|Compound:9061122|FD-Score:-4.00|P-value:1.57E-4 ID:SGTC_2358|Compound:9037157|FD-Score:5.31|P-value:5.51E-7 ID:SGTC_2452|Compound:5863136|FD-Score:3.62|P-value:4.30E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.49|P-value:6.63E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:4.48|P-value:1.96E-5 ID:SGTC_2674|Compound:ligustilide|FD-Score:5.04|P-value:1.88E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.83|P-value:2.12E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.61|P-value:4.45E-4 ID:SGTC_2821|Compound:7996764|FD-Score:6.89|P-value:1.43E-10 ID:SGTC_2822|Compound:7994718|FD-Score:-4.24|P-value:6.62E-5 ID:SGTC_2823|Compound:7987624|FD-Score:-4.06|P-value:1.26E-4 ID:SGTC_2957|Compound:9076800|FD-Score:3.37|P-value:9.48E-4 ID:SGTC_3105|Compound:9121040|FD-Score:-3.68|P-value:4.67E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.43|P-value:7.92E-4 ID:SGTC_3120|Compound:9124077|FD-Score:4.30|P-value:3.85E-5 ID:SGTC_3133|Compound:9127736|FD-Score:4.43|P-value:2.37E-5 ID:SGTC_3210|Compound:9128157|FD-Score:3.64|P-value:4.01E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-8.31|P-value:2.57E-14

Top fitness defect scores for YGR162W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2129 girgensonine 9.31 121.0 µM 9.02E-18 heme biosynthesis & mitochondrial translocase 2 6.58E-21
2 SGTC_625 k213-0058 7.62 121.0 µM 1.55E-12 translation 15 1.24E-14
3 SGTC_2821 7996764 6.89 71.4 µM 1.43E-10 3 2.79E-12
4 SGTC_1781 st038725 6.70 66.3 µM 4.34E-10 NEO1 46 1.06E-11
5 SGTC_832 4083-0019 6.52 164.0 µM 1.21E-9 translation 14 3.61E-11
6 SGTC_831 k061-1017 5.87 217.0 µM 3.81E-8 translation 11 2.21E-9
7 SGTC_800 3448-7362 5.34 155.0 µM 4.89E-7 translation 72 4.65E-8
8 SGTC_2358 9037157 5.31 4.0 µM 5.51E-7 9 5.37E-8
9 SGTC_366 0181-0206 5.16 4.3 µM 1.11E-6 Golgi 13 1.24E-7
10 SGTC_2674 ligustilide 5.04 100.0 µM 1.88E-6 2 2.31E-7
11 SGTC_118 0083-0097 4.96 135.3 µM 2.73E-6 translation 61 3.61E-7
12 SGTC_1728 st035614 4.79 33.8 µM 5.51E-6 26 8.32E-7
13 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 4.48 13.2 µM 1.96E-5 15 3.76E-6
14 SGTC_3133 9127736 4.43 49.5 µM 2.37E-5 13 4.71E-6
15 SGTC_1942 st076330 4.40 65.2 µM 2.68E-5 19 5.45E-6
16 SGTC_104 0074-0074 4.32 591.0 µM 3.63E-5 6 7.83E-6
17 SGTC_3120 9124077 4.30 49.5 µM 3.85E-5 15 8.40E-6
18 SGTC_1365 1672-3266 4.24 55.4 µM 4.85E-5 11 1.10E-5
19 SGTC_582 0987-0078 4.07 156.0 µM 9.12E-5 calcium & mitochondrial duress 63 2.33E-5
20 SGTC_2688 kanamycin b 3.83 96.7 µM 2.12E-4 calcium & mitochondrial duress 391 6.34E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.285 1.23E-63 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.230 1.84E-41 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.215 2.25E-36 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.213 8.85E-36 YBL071C-B_p YBL071C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.187 7.06E-28 YJR145C RPS4A hom Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.175 1.41E-24 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.173 7.51E-24 YLR068W FYV7 hom Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
0.173 7.15E-24 YGR271C-A EFG1 hom Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
0.171 1.94E-23 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.166 3.60E-22 YKR057W RPS21A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
0.161 7.21E-21 YOL121C RPS19A hom Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
0.158 3.28E-20 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.157 7.66E-20 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.155 1.38E-19 YOR078W BUD21 hom Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.152 8.39E-19 YDR174W HMO1 hom Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase