YGR169C / PUS6

tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR169C / PUS6

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:-3.22|P-value:9.15E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.83|P-value:8.63E-9 ID:SGTC_42|Compound:0133-0086|FD-Score:-4.48|P-value:8.40E-8 ID:SGTC_358|Compound:0075-0058|FD-Score:4.50|P-value:5.07E-8 ID:SGTC_397|Compound:arbidol|FD-Score:2.89|P-value:2.92E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.14|P-value:9.46E-5 ID:SGTC_493|Compound:zm226600|FD-Score:-5.08|P-value:1.55E-9 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.28|P-value:6.68E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:-5.18|P-value:7.26E-10 ID:SGTC_532|Compound:1548-0774|FD-Score:2.75|P-value:5.43E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.26|P-value:5.37E-5 ID:SGTC_664|Compound:1222-0054|FD-Score:-2.76|P-value:6.65E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-2.67|P-value:9.55E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:2.90|P-value:2.78E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:2.61|P-value:9.56E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.57|P-value:1.14E-5 ID:SGTC_1073|Compound:triclabendazole|FD-Score:2.69|P-value:7.00E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.99|P-value:1.64E-6 ID:SGTC_1192|Compound:1488-0095|FD-Score:-3.42|P-value:3.48E-5 ID:SGTC_1197|Compound:1601-0112|FD-Score:-2.67|P-value:9.85E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.04|P-value:2.00E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:2.75|P-value:5.28E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.83|P-value:5.06E-4 ID:SGTC_1498|Compound:4478-3555|FD-Score:3.03|P-value:1.60E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.32|P-value:5.69E-5 ID:SGTC_1689|Compound:st020265|FD-Score:2.67|P-value:7.30E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.33|P-value:3.85E-5 ID:SGTC_1704|Compound:st028805|FD-Score:-2.81|P-value:5.54E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-3.97|P-value:1.81E-6 ID:SGTC_1735|Compound:st034310|FD-Score:3.09|P-value:1.21E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.27|P-value:5.24E-5 ID:SGTC_1802|Compound:st050112|FD-Score:-3.13|P-value:1.38E-4 ID:SGTC_1810|Compound:st050138|FD-Score:2.91|P-value:2.69E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-3.05|P-value:1.98E-4 ID:SGTC_2087|Compound:5276107|FD-Score:-2.68|P-value:9.29E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.03|P-value:2.12E-4 ID:SGTC_2290|Compound:7904846|FD-Score:-3.22|P-value:8.98E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.54|P-value:1.36E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.45|P-value:2.16E-5 ID:SGTC_2327|Compound:6189656|FD-Score:3.25|P-value:5.74E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.80|P-value:3.29E-6 ID:SGTC_2337|Compound:9009472|FD-Score:4.26|P-value:2.25E-7 ID:SGTC_2368|Compound:9071271|FD-Score:-2.70|P-value:8.49E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.12|P-value:1.07E-4 ID:SGTC_2462|Compound:5162487|FD-Score:2.65|P-value:8.00E-4 ID:SGTC_2481|Compound:5478948|FD-Score:2.77|P-value:4.84E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:2.63|P-value:8.66E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.80|P-value:1.04E-8 ID:SGTC_2641|Compound:levomentholum|FD-Score:6.31|P-value:4.48E-14 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.57|P-value:1.61E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.95|P-value:2.23E-4 ID:SGTC_2802|Compound:7925815|FD-Score:-4.04|P-value:1.23E-6 ID:SGTC_2815|Compound:7968870|FD-Score:-3.04|P-value:2.06E-4 ID:SGTC_3009|Compound:9080819|FD-Score:2.69|P-value:6.97E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.42|P-value:3.47E-5 ID:SGTC_3291|Compound:9121177|FD-Score:3.16|P-value:8.87E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.22|P-value:9.15E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.83|P-value:8.63E-9 ID:SGTC_42|Compound:0133-0086|FD-Score:-4.48|P-value:8.40E-8 ID:SGTC_358|Compound:0075-0058|FD-Score:4.50|P-value:5.07E-8 ID:SGTC_397|Compound:arbidol|FD-Score:2.89|P-value:2.92E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.14|P-value:9.46E-5 ID:SGTC_493|Compound:zm226600|FD-Score:-5.08|P-value:1.55E-9 ID:SGTC_501|Compound:nsc-95397|FD-Score:-3.28|P-value:6.68E-5 ID:SGTC_502|Compound:l-744,832|FD-Score:-5.18|P-value:7.26E-10 ID:SGTC_532|Compound:1548-0774|FD-Score:2.75|P-value:5.43E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.26|P-value:5.37E-5 ID:SGTC_664|Compound:1222-0054|FD-Score:-2.76|P-value:6.65E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-2.67|P-value:9.55E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:2.90|P-value:2.78E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:2.61|P-value:9.56E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.57|P-value:1.14E-5 ID:SGTC_1073|Compound:triclabendazole|FD-Score:2.69|P-value:7.00E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.99|P-value:1.64E-6 ID:SGTC_1192|Compound:1488-0095|FD-Score:-3.42|P-value:3.48E-5 ID:SGTC_1197|Compound:1601-0112|FD-Score:-2.67|P-value:9.85E-4 ID:SGTC_1227|Compound:3909-8010|FD-Score:-3.04|P-value:2.00E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:2.75|P-value:5.28E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-2.83|P-value:5.06E-4 ID:SGTC_1498|Compound:4478-3555|FD-Score:3.03|P-value:1.60E-4 ID:SGTC_1638|Compound:st008370|FD-Score:-3.32|P-value:5.69E-5 ID:SGTC_1689|Compound:st020265|FD-Score:2.67|P-value:7.30E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.33|P-value:3.85E-5 ID:SGTC_1704|Compound:st028805|FD-Score:-2.81|P-value:5.54E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-3.97|P-value:1.81E-6 ID:SGTC_1735|Compound:st034310|FD-Score:3.09|P-value:1.21E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.27|P-value:5.24E-5 ID:SGTC_1802|Compound:st050112|FD-Score:-3.13|P-value:1.38E-4 ID:SGTC_1810|Compound:st050138|FD-Score:2.91|P-value:2.69E-4 ID:SGTC_1848|Compound:st056003|FD-Score:-3.05|P-value:1.98E-4 ID:SGTC_2087|Compound:5276107|FD-Score:-2.68|P-value:9.29E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.03|P-value:2.12E-4 ID:SGTC_2290|Compound:7904846|FD-Score:-3.22|P-value:8.98E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.54|P-value:1.36E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.45|P-value:2.16E-5 ID:SGTC_2327|Compound:6189656|FD-Score:3.25|P-value:5.74E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.80|P-value:3.29E-6 ID:SGTC_2337|Compound:9009472|FD-Score:4.26|P-value:2.25E-7 ID:SGTC_2368|Compound:9071271|FD-Score:-2.70|P-value:8.49E-4 ID:SGTC_2434|Compound:5670830|FD-Score:3.12|P-value:1.07E-4 ID:SGTC_2462|Compound:5162487|FD-Score:2.65|P-value:8.00E-4 ID:SGTC_2481|Compound:5478948|FD-Score:2.77|P-value:4.84E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:2.63|P-value:8.66E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.80|P-value:1.04E-8 ID:SGTC_2641|Compound:levomentholum|FD-Score:6.31|P-value:4.48E-14 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.57|P-value:1.61E-5 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.95|P-value:2.23E-4 ID:SGTC_2802|Compound:7925815|FD-Score:-4.04|P-value:1.23E-6 ID:SGTC_2815|Compound:7968870|FD-Score:-3.04|P-value:2.06E-4 ID:SGTC_3009|Compound:9080819|FD-Score:2.69|P-value:6.97E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.42|P-value:3.47E-5 ID:SGTC_3291|Compound:9121177|FD-Score:3.16|P-value:8.87E-5

Top fitness defect scores for YGR169C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2641 levomentholum 6.31 100.0 µM 4.48E-14 2 1.39E-10
2 SGTC_358 0075-0058 4.50 9.4 µM 5.07E-8 43 3.44E-6
3 SGTC_2337 9009472 4.26 200.0 µM 2.25E-7 23 1.02E-5
4 SGTC_2336 9001819 3.80 198.7 µM 3.29E-6 heme biosynthesis & mitochondrial translocase 61 7.18E-5
5 SGTC_987 1497-1013 3.57 404.0 µM 1.14E-5 18 1.77E-4
6 SGTC_2297 7971671 3.54 152.3 µM 1.36E-5 RNA processing & uracil transport 42 2.03E-4
7 SGTC_2322 7413319 3.45 38.2 µM 2.16E-5 ERAD & cell cycle 192 2.84E-4
8 SGTC_1698 5-fluorocytosine 3.33 1.1 µM 3.85E-5 exosome 18 4.33E-4
9 SGTC_1748 st037455 3.27 53.6 µM 5.24E-5 63 5.42E-4
10 SGTC_535 2293-4129 3.26 48.2 µM 5.37E-5 NEO1 102 5.52E-4
11 SGTC_2327 6189656 3.25 200.0 µM 5.74E-5 49 5.80E-4
12 SGTC_3291 9121177 3.16 71.4 µM 8.87E-5 35 7.97E-4
13 SGTC_431 k089-0097 3.14 107.0 µM 9.46E-5 iron homeostasis 88 8.36E-4
14 SGTC_2434 5670830 3.12 169.9 µM 1.07E-4 64 9.15E-4
15 SGTC_1735 st034310 3.09 56.8 µM 1.21E-4 Golgi 63 0.00100
16 SGTC_1498 4478-3555 3.03 97.6 µM 1.60E-4 22 0.00123
17 SGTC_2697 mebhydrolin 2.95 72.4 µM 2.23E-4 NEO1 133 0.00157
18 SGTC_1810 st050138 2.91 53.0 µM 2.69E-4 PDR1 38 0.00180
19 SGTC_851 0394-0702 2.90 453.0 µM 2.78E-4 mitochondrial processes 51 0.00184
20 SGTC_397 arbidol 2.89 50.0 µM 2.92E-4 cell wall 70 0.00191

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.218 1.74E-37 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.125 3.35E-13 YIL073C SPO22 hom Meiosis-specific protein essential for chromosome synapsis, involved in completion of nuclear divisions during meiosis; induced early in meiosis
0.122 1.14E-12 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
0.121 2.09E-12 YHR131C_p YHR131C_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study
0.121 2.12E-12 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.118 5.99E-12 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.118 6.76E-12 YOR302W YOR302W hom CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA
0.113 4.56E-11 YDR508C GNP1 hom High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication
0.106 6.83E-10 YLR305C STT4 het Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
0.106 7.53E-10 YDR285W ZIP1 hom Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate
0.104 1.75E-9 YBR149W ARA1 hom NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
0.103 2.33E-9 YDR420W HKR1 hom Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
0.102 3.20E-9 YLR360W VPS38 hom Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated
0.101 4.72E-9 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
0.101 5.36E-9 YGR176W_d YGR176W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data