YGR174C / CBP4

Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR174C / CBP4

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:6.26|P-value:5.01E-11 ID:SGTC_67|Compound:0335-0847|FD-Score:-5.08|P-value:7.15E-8 ID:SGTC_127|Compound:0180-0333|FD-Score:3.54|P-value:1.28E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:5.62|P-value:3.13E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:3.35|P-value:2.69E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.23|P-value:6.33E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:19.50|P-value:6.32E-91 ID:SGTC_359|Compound:0081-0058|FD-Score:3.57|P-value:1.11E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.57|P-value:1.13E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.07|P-value:7.27E-4 ID:SGTC_616|Compound:4092-0450|FD-Score:3.02|P-value:9.23E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:3.97|P-value:2.06E-5 ID:SGTC_666|Compound:0272-0154|FD-Score:5.06|P-value:8.39E-8 ID:SGTC_766|Compound:1391-0721|FD-Score:3.46|P-value:1.76E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:4.01|P-value:1.72E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.10|P-value:6.58E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.81|P-value:4.04E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:-3.41|P-value:2.05E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:-3.38|P-value:2.30E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:4.75|P-value:4.49E-7 ID:SGTC_892|Compound:0929-0076|FD-Score:3.51|P-value:1.44E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:4.23|P-value:6.24E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:6.17|P-value:9.21E-11 ID:SGTC_977|Compound:1348-1378|FD-Score:5.74|P-value:1.49E-9 ID:SGTC_986|Compound:1493-0483|FD-Score:3.38|P-value:2.43E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.51|P-value:1.60E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:-4.15|P-value:8.74E-6 ID:SGTC_1163|Compound:k060-0048|FD-Score:-3.37|P-value:2.43E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.26|P-value:3.81E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.46|P-value:1.78E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.06|P-value:7.98E-4 ID:SGTC_1320|Compound:1313-0481|FD-Score:6.35|P-value:2.59E-11 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.45|P-value:1.87E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.12|P-value:6.16E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.05|P-value:8.25E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.90|P-value:2.77E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:3.01|P-value:9.45E-4 ID:SGTC_1635|Compound:st007035|FD-Score:15.50|P-value:3.97E-58 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:4.67|P-value:7.10E-7 ID:SGTC_1739|Compound:st037196|FD-Score:3.61|P-value:9.56E-5 ID:SGTC_1747|Compound:st037496|FD-Score:-3.82|P-value:3.77E-5 ID:SGTC_1758|Compound:st042942|FD-Score:3.23|P-value:4.27E-4 ID:SGTC_1992|Compound:st073643|FD-Score:-3.18|P-value:4.93E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.74|P-value:5.33E-5 ID:SGTC_2185|Compound:6113692|FD-Score:3.11|P-value:6.51E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-3.25|P-value:3.84E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.39|P-value:2.28E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:4.25|P-value:5.61E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.77|P-value:4.86E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:6.85|P-value:7.02E-13 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.72|P-value:6.15E-5 ID:SGTC_2711|Compound:st079103|FD-Score:4.34|P-value:3.75E-6 ID:SGTC_2716|Compound:st080858|FD-Score:3.98|P-value:2.01E-5 ID:SGTC_2717|Compound:st081029|FD-Score:4.90|P-value:2.08E-7 ID:SGTC_2853|Compound:9015042|FD-Score:-4.41|P-value:2.42E-6 ID:SGTC_2855|Compound:9016525|FD-Score:3.13|P-value:6.21E-4 ID:SGTC_2856|Compound:9022925|FD-Score:7.72|P-value:7.30E-16 ID:SGTC_15|Compound:0266-0086|FD-Score:6.26|P-value:5.01E-11 ID:SGTC_67|Compound:0335-0847|FD-Score:-5.08|P-value:7.15E-8 ID:SGTC_127|Compound:0180-0333|FD-Score:3.54|P-value:1.28E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:5.62|P-value:3.13E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:3.35|P-value:2.69E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.23|P-value:6.33E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:19.50|P-value:6.32E-91 ID:SGTC_359|Compound:0081-0058|FD-Score:3.57|P-value:1.11E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.57|P-value:1.13E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.07|P-value:7.27E-4 ID:SGTC_616|Compound:4092-0450|FD-Score:3.02|P-value:9.23E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:3.97|P-value:2.06E-5 ID:SGTC_666|Compound:0272-0154|FD-Score:5.06|P-value:8.39E-8 ID:SGTC_766|Compound:1391-0721|FD-Score:3.46|P-value:1.76E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:4.01|P-value:1.72E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.10|P-value:6.58E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.81|P-value:4.04E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:-3.41|P-value:2.05E-4 ID:SGTC_795|Compound:0685-0265|FD-Score:-3.38|P-value:2.30E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:4.75|P-value:4.49E-7 ID:SGTC_892|Compound:0929-0076|FD-Score:3.51|P-value:1.44E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:4.23|P-value:6.24E-6 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:6.17|P-value:9.21E-11 ID:SGTC_977|Compound:1348-1378|FD-Score:5.74|P-value:1.49E-9 ID:SGTC_986|Compound:1493-0483|FD-Score:3.38|P-value:2.43E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:4.51|P-value:1.60E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:-4.15|P-value:8.74E-6 ID:SGTC_1163|Compound:k060-0048|FD-Score:-3.37|P-value:2.43E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.26|P-value:3.81E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.46|P-value:1.78E-4 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.06|P-value:7.98E-4 ID:SGTC_1320|Compound:1313-0481|FD-Score:6.35|P-value:2.59E-11 ID:SGTC_1325|Compound:1431-0015|FD-Score:3.45|P-value:1.87E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.12|P-value:6.16E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:3.05|P-value:8.25E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.90|P-value:2.77E-5 ID:SGTC_1570|Compound:verbenone|FD-Score:3.01|P-value:9.45E-4 ID:SGTC_1635|Compound:st007035|FD-Score:15.50|P-value:3.97E-58 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:4.67|P-value:7.10E-7 ID:SGTC_1739|Compound:st037196|FD-Score:3.61|P-value:9.56E-5 ID:SGTC_1747|Compound:st037496|FD-Score:-3.82|P-value:3.77E-5 ID:SGTC_1758|Compound:st042942|FD-Score:3.23|P-value:4.27E-4 ID:SGTC_1992|Compound:st073643|FD-Score:-3.18|P-value:4.93E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.74|P-value:5.33E-5 ID:SGTC_2185|Compound:6113692|FD-Score:3.11|P-value:6.51E-4 ID:SGTC_2397|Compound:5161689|FD-Score:-3.25|P-value:3.84E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.39|P-value:2.28E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:4.25|P-value:5.61E-6 ID:SGTC_2625|Compound:euphol|FD-Score:3.77|P-value:4.86E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:6.85|P-value:7.02E-13 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.72|P-value:6.15E-5 ID:SGTC_2711|Compound:st079103|FD-Score:4.34|P-value:3.75E-6 ID:SGTC_2716|Compound:st080858|FD-Score:3.98|P-value:2.01E-5 ID:SGTC_2717|Compound:st081029|FD-Score:4.90|P-value:2.08E-7 ID:SGTC_2853|Compound:9015042|FD-Score:-4.41|P-value:2.42E-6 ID:SGTC_2855|Compound:9016525|FD-Score:3.13|P-value:6.21E-4 ID:SGTC_2856|Compound:9022925|FD-Score:7.72|P-value:7.30E-16

Top fitness defect scores for YGR174C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 19.50 5.3 µM 6.32E-91 59 3.56E-85
2 SGTC_1635 st007035 15.50 42.4 µM 3.97E-58 35 1.67E-54
3 SGTC_2856 9022925 7.72 19.5 µM 7.30E-16 1 5.80E-15
4 SGTC_2627 acetyl isogambogic acid 6.85 100.0 µM 7.02E-13 TSC3-RPN4 10 3.60E-12
5 SGTC_1320 1313-0481 6.35 95.3 µM 2.59E-11 4 1.06E-10
6 SGTC_15 0266-0086 6.26 27.1 µM 5.01E-11 endomembrane recycling 16 1.96E-10
7 SGTC_920 mycophenolic acid 6.17 780.4 nM 9.21E-11 2 3.47E-10
8 SGTC_977 1348-1378 5.74 135.0 µM 1.49E-9 5 4.72E-9
9 SGTC_134 0269-0018 5.62 6.9 µM 3.13E-9 87 9.44E-9
10 SGTC_666 0272-0154 5.06 136.0 µM 8.39E-8 cell wall 28 2.06E-7
11 SGTC_2717 st081029 4.90 62.3 µM 2.08E-7 5 4.83E-7
12 SGTC_824 1304-0008 4.75 100.0 µM 4.49E-7 DNA damage response 24 9.94E-7
13 SGTC_1698 5-fluorocytosine 4.67 1.1 µM 7.10E-7 exosome 4 1.53E-6
14 SGTC_1149 3909-7961 4.51 23.3 µM 1.60E-6 7 3.28E-6
15 SGTC_2711 st079103 4.34 59.1 µM 3.75E-6 4 7.28E-6
16 SGTC_2576 guaiol 4.25 23.1 µM 5.61E-6 12 1.06E-5
17 SGTC_919 tnp00227 4.23 403.6 nM 6.24E-6 RNA pol III & RNase P/MRP 25 1.17E-5
18 SGTC_159 3970-0959 4.23 50.0 µM 6.33E-6 65 1.19E-5
19 SGTC_781 4335-2806 4.01 94.4 µM 1.72E-5 19 3.04E-5
20 SGTC_2716 st080858 3.98 38.6 µM 2.01E-5 60S ribosome export 21 3.51E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.551 2.73E-266 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.524 1.49E-235 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.519 1.80E-230 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.514 7.35E-226 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.513 1.24E-224 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.506 3.94E-218 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.501 2.61E-212 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.499 1.44E-210 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.497 1.29E-208 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.496 3.52E-208 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.493 4.08E-205 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.489 1.83E-201 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.480 2.74E-192 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.472 8.79E-186 YLR038C COX12 hom Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
0.469 1.62E-183 YER058W PET117 hom Protein required for assembly of cytochrome c oxidase