YGR200C / ELP2

Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR200C / ELP2

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:4.72|P-value:8.31E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.04|P-value:1.16E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-4.35|P-value:3.65E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.55|P-value:6.16E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-4.40|P-value:3.04E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-8.40|P-value:9.95E-15 ID:SGTC_522|Compound:1273-0010|FD-Score:4.06|P-value:1.06E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.39|P-value:4.49E-7 ID:SGTC_680|Compound:0417-1641|FD-Score:4.36|P-value:3.50E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:-4.28|P-value:4.75E-5 ID:SGTC_706|Compound:4262-0057|FD-Score:3.60|P-value:5.06E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.29|P-value:6.88E-7 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.81|P-value:2.55E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.63|P-value:4.59E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.56|P-value:5.82E-4 ID:SGTC_1609|Compound:st001574|FD-Score:5.43|P-value:3.71E-7 ID:SGTC_1613|Compound:st002381|FD-Score:3.92|P-value:1.73E-4 ID:SGTC_1751|Compound:st044839|FD-Score:-5.11|P-value:1.61E-6 ID:SGTC_1754|Compound:st042065|FD-Score:6.27|P-value:5.51E-9 ID:SGTC_1763|Compound:st044482|FD-Score:5.40|P-value:4.15E-7 ID:SGTC_1800|Compound:st050079|FD-Score:3.91|P-value:1.81E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.67|P-value:1.02E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.49|P-value:7.28E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:10.20|P-value:8.76E-21 ID:SGTC_1849|Compound:st056239|FD-Score:5.44|P-value:3.56E-7 ID:SGTC_1972|Compound:st070201|FD-Score:-3.68|P-value:4.00E-4 ID:SGTC_2074|Compound:5212007|FD-Score:-4.11|P-value:9.04E-5 ID:SGTC_2143|Compound:5623677|FD-Score:3.48|P-value:7.59E-4 ID:SGTC_2361|Compound:9034121|FD-Score:-3.53|P-value:6.43E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-4.53|P-value:1.85E-5 ID:SGTC_2365|Compound:9042475|FD-Score:4.09|P-value:9.49E-5 ID:SGTC_2437|Compound:5741531|FD-Score:-4.28|P-value:4.81E-5 ID:SGTC_2442|Compound:5158906|FD-Score:4.11|P-value:8.98E-5 ID:SGTC_2450|Compound:5739737|FD-Score:-5.14|P-value:1.38E-6 ID:SGTC_2455|Compound:5377712|FD-Score:-3.40|P-value:9.64E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.29|P-value:4.65E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.40|P-value:1.61E-21 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.08|P-value:8.51E-14 ID:SGTC_2713|Compound:st079149|FD-Score:3.64|P-value:4.55E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:-3.63|P-value:4.72E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.05|P-value:1.78E-8 ID:SGTC_2947|Compound:9072639|FD-Score:3.85|P-value:2.24E-4 ID:SGTC_2966|Compound:9084041|FD-Score:4.67|P-value:1.03E-5 ID:SGTC_3058|Compound:9093861|FD-Score:-3.42|P-value:9.05E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.59|P-value:5.40E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.42|P-value:2.82E-5 ID:SGTC_3140|Compound:9094243|FD-Score:5.37|P-value:4.88E-7 ID:SGTC_3142|Compound:9086498|FD-Score:-3.82|P-value:2.51E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-4.28|P-value:4.86E-5 ID:SGTC_3203|Compound:9113543|FD-Score:-3.61|P-value:5.09E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.70|P-value:3.81E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-3.44|P-value:8.53E-4 ID:SGTC_3246|Compound:9135460|FD-Score:-5.15|P-value:1.35E-6 ID:SGTC_3248|Compound:9135562|FD-Score:3.72|P-value:3.40E-4 ID:SGTC_3282|Compound:9108498|FD-Score:4.04|P-value:1.16E-4 ID:SGTC_3286|Compound:9117135|FD-Score:3.40|P-value:9.63E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.77|P-value:2.91E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.72|P-value:8.31E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.04|P-value:1.16E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-4.35|P-value:3.65E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.55|P-value:6.16E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-4.40|P-value:3.04E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-8.40|P-value:9.95E-15 ID:SGTC_522|Compound:1273-0010|FD-Score:4.06|P-value:1.06E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.39|P-value:4.49E-7 ID:SGTC_680|Compound:0417-1641|FD-Score:4.36|P-value:3.50E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:-4.28|P-value:4.75E-5 ID:SGTC_706|Compound:4262-0057|FD-Score:3.60|P-value:5.06E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.29|P-value:6.88E-7 ID:SGTC_904|Compound:1141-0088|FD-Score:-3.81|P-value:2.55E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.63|P-value:4.59E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.56|P-value:5.82E-4 ID:SGTC_1609|Compound:st001574|FD-Score:5.43|P-value:3.71E-7 ID:SGTC_1613|Compound:st002381|FD-Score:3.92|P-value:1.73E-4 ID:SGTC_1751|Compound:st044839|FD-Score:-5.11|P-value:1.61E-6 ID:SGTC_1754|Compound:st042065|FD-Score:6.27|P-value:5.51E-9 ID:SGTC_1763|Compound:st044482|FD-Score:5.40|P-value:4.15E-7 ID:SGTC_1800|Compound:st050079|FD-Score:3.91|P-value:1.81E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.67|P-value:1.02E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.49|P-value:7.28E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:10.20|P-value:8.76E-21 ID:SGTC_1849|Compound:st056239|FD-Score:5.44|P-value:3.56E-7 ID:SGTC_1972|Compound:st070201|FD-Score:-3.68|P-value:4.00E-4 ID:SGTC_2074|Compound:5212007|FD-Score:-4.11|P-value:9.04E-5 ID:SGTC_2143|Compound:5623677|FD-Score:3.48|P-value:7.59E-4 ID:SGTC_2361|Compound:9034121|FD-Score:-3.53|P-value:6.43E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-4.53|P-value:1.85E-5 ID:SGTC_2365|Compound:9042475|FD-Score:4.09|P-value:9.49E-5 ID:SGTC_2437|Compound:5741531|FD-Score:-4.28|P-value:4.81E-5 ID:SGTC_2442|Compound:5158906|FD-Score:4.11|P-value:8.98E-5 ID:SGTC_2450|Compound:5739737|FD-Score:-5.14|P-value:1.38E-6 ID:SGTC_2455|Compound:5377712|FD-Score:-3.40|P-value:9.64E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.29|P-value:4.65E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:10.40|P-value:1.61E-21 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.08|P-value:8.51E-14 ID:SGTC_2713|Compound:st079149|FD-Score:3.64|P-value:4.55E-4 ID:SGTC_2752|Compound:diclazuril|FD-Score:-3.63|P-value:4.72E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-6.05|P-value:1.78E-8 ID:SGTC_2947|Compound:9072639|FD-Score:3.85|P-value:2.24E-4 ID:SGTC_2966|Compound:9084041|FD-Score:4.67|P-value:1.03E-5 ID:SGTC_3058|Compound:9093861|FD-Score:-3.42|P-value:9.05E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.59|P-value:5.40E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.42|P-value:2.82E-5 ID:SGTC_3140|Compound:9094243|FD-Score:5.37|P-value:4.88E-7 ID:SGTC_3142|Compound:9086498|FD-Score:-3.82|P-value:2.51E-4 ID:SGTC_3201|Compound:9113999|FD-Score:-4.28|P-value:4.86E-5 ID:SGTC_3203|Compound:9113543|FD-Score:-3.61|P-value:5.09E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.70|P-value:3.81E-4 ID:SGTC_3219|Compound:9131112|FD-Score:-3.44|P-value:8.53E-4 ID:SGTC_3246|Compound:9135460|FD-Score:-5.15|P-value:1.35E-6 ID:SGTC_3248|Compound:9135562|FD-Score:3.72|P-value:3.40E-4 ID:SGTC_3282|Compound:9108498|FD-Score:4.04|P-value:1.16E-4 ID:SGTC_3286|Compound:9117135|FD-Score:3.40|P-value:9.63E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.77|P-value:2.91E-4

Top fitness defect scores for YGR200C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2679 rapamycin 10.40 1.0 nM 1.61E-21 24 1.70E-25
2 SGTC_1844 rapamycin 10.20 4.0 nM 8.76E-21 37 1.30E-24
3 SGTC_2688 kanamycin b 8.08 96.7 µM 8.51E-14 calcium & mitochondrial duress 61 3.16E-16
4 SGTC_1754 st042065 6.27 16.1 µM 5.51E-9 NEO1-PIK1 92 1.85E-10
5 SGTC_1849 st056239 5.44 19.2 µM 3.56E-7 RPP1 & pyrimidine depletion 4 2.72E-8
6 SGTC_1609 st001574 5.43 46.4 µM 3.71E-7 2 2.85E-8
7 SGTC_1763 st044482 5.40 94.5 µM 4.15E-7 copper-dependent oxidative stress 7 3.26E-8
8 SGTC_3140 9094243 5.37 49.5 µM 4.88E-7 8 3.96E-8
9 SGTC_800 3448-7362 5.29 155.0 µM 6.88E-7 translation 76 5.97E-8
10 SGTC_37 0566-0070 4.72 1.6 µM 8.31E-6 15 1.17E-6
11 SGTC_1803 st050068 4.67 52.4 µM 1.02E-5 NEO1-PIK1 130 1.49E-6
12 SGTC_2966 9084041 4.67 48.0 µM 1.03E-5 18 1.52E-6
13 SGTC_680 0417-1641 4.36 114.0 µM 3.50E-5 RPP1 & pyrimidine depletion 7 6.51E-6
14 SGTC_2582 agaric acid 4.29 100.0 µM 4.65E-5 redox potentiating 279 9.13E-6
15 SGTC_2442 5158906 4.11 48.5 µM 8.98E-5 17 2.00E-5
16 SGTC_2365 9042475 4.09 200.0 µM 9.49E-5 33 2.14E-5
17 SGTC_522 1273-0010 4.06 11.6 µM 1.06E-4 60S ribosome export 22 2.43E-5
18 SGTC_3282 9108498 4.04 15.5 µM 1.16E-4 28 2.72E-5
19 SGTC_1613 st002381 3.92 52.1 µM 1.73E-4 24 4.36E-5
20 SGTC_1800 st050079 3.91 29.1 µM 1.81E-4 NEO1-PIK1 126 4.62E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.406 4.90E-133 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.293 2.42E-67 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.281 8.21E-62 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.280 1.94E-61 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.280 1.98E-61 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.274 9.51E-59 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.272 7.89E-58 YHR111W UBA4 hom Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.268 2.54E-56 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.266 2.14E-55 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.258 3.20E-52 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.249 1.16E-48 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.246 1.69E-47 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.239 7.40E-45 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.233 1.89E-42 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.221 2.05E-38 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity