YGR202C / PCT1

Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR202C / PCT1

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:3.73|P-value:2.51E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:6.73|P-value:1.69E-13 ID:SGTC_146|Compound:3448-8650|FD-Score:2.97|P-value:5.94E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.28|P-value:1.78E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.75|P-value:3.32E-26 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.11|P-value:2.58E-8 ID:SGTC_439|Compound:amiodarone|FD-Score:4.01|P-value:6.52E-6 ID:SGTC_535|Compound:2293-4129|FD-Score:4.03|P-value:6.00E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:3.45|P-value:8.75E-5 ID:SGTC_699|Compound:0375-0592|FD-Score:3.46|P-value:8.09E-5 ID:SGTC_705|Compound:1124-0212|FD-Score:3.16|P-value:2.80E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-4.48|P-value:9.05E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.03|P-value:6.74E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:2.99|P-value:5.45E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:2.93|P-value:6.98E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.12|P-value:4.68E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.08|P-value:3.97E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.05|P-value:4.39E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.23|P-value:2.14E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:4.36|P-value:1.09E-6 ID:SGTC_988|Compound:1497-1057|FD-Score:3.27|P-value:1.80E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.44|P-value:1.28E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.02|P-value:6.88E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.24|P-value:2.94E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.19|P-value:2.52E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.12|P-value:3.35E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.06|P-value:4.17E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.08|P-value:3.85E-4 ID:SGTC_1750|Compound:st081859|FD-Score:8.29|P-value:1.70E-19 ID:SGTC_1754|Compound:st042065|FD-Score:8.69|P-value:3.06E-21 ID:SGTC_1800|Compound:st050079|FD-Score:2.92|P-value:7.17E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.31|P-value:1.58E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.20|P-value:2.46E-4 ID:SGTC_2107|Compound:5363541|FD-Score:2.92|P-value:7.29E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.18|P-value:3.77E-4 ID:SGTC_2335|Compound:9018011|FD-Score:2.89|P-value:8.00E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.90|P-value:7.84E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-2.95|P-value:9.08E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:3.28|P-value:1.76E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-3.06|P-value:5.98E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.55|P-value:6.61E-13 ID:SGTC_2794|Compound:7785382|FD-Score:4.32|P-value:1.38E-6 ID:SGTC_2961|Compound:9083290|FD-Score:-3.14|P-value:4.42E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-2.93|P-value:9.53E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.18|P-value:3.70E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.73|P-value:2.51E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:6.73|P-value:1.69E-13 ID:SGTC_146|Compound:3448-8650|FD-Score:2.97|P-value:5.94E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.28|P-value:1.78E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.75|P-value:3.32E-26 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.11|P-value:2.58E-8 ID:SGTC_439|Compound:amiodarone|FD-Score:4.01|P-value:6.52E-6 ID:SGTC_535|Compound:2293-4129|FD-Score:4.03|P-value:6.00E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:3.45|P-value:8.75E-5 ID:SGTC_699|Compound:0375-0592|FD-Score:3.46|P-value:8.09E-5 ID:SGTC_705|Compound:1124-0212|FD-Score:3.16|P-value:2.80E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-4.48|P-value:9.05E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.03|P-value:6.74E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:2.99|P-value:5.45E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:2.93|P-value:6.98E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.12|P-value:4.68E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.08|P-value:3.97E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:3.05|P-value:4.39E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.23|P-value:2.14E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:4.36|P-value:1.09E-6 ID:SGTC_988|Compound:1497-1057|FD-Score:3.27|P-value:1.80E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:-3.44|P-value:1.28E-4 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.02|P-value:6.88E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.24|P-value:2.94E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.19|P-value:2.52E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.12|P-value:3.35E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.06|P-value:4.17E-4 ID:SGTC_1634|Compound:st006576|FD-Score:3.08|P-value:3.85E-4 ID:SGTC_1750|Compound:st081859|FD-Score:8.29|P-value:1.70E-19 ID:SGTC_1754|Compound:st042065|FD-Score:8.69|P-value:3.06E-21 ID:SGTC_1800|Compound:st050079|FD-Score:2.92|P-value:7.17E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.31|P-value:1.58E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.20|P-value:2.46E-4 ID:SGTC_2107|Compound:5363541|FD-Score:2.92|P-value:7.29E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-3.18|P-value:3.77E-4 ID:SGTC_2335|Compound:9018011|FD-Score:2.89|P-value:8.00E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.90|P-value:7.84E-4 ID:SGTC_2459|Compound:5811157|FD-Score:-2.95|P-value:9.08E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:3.28|P-value:1.76E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:-3.06|P-value:5.98E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.55|P-value:6.61E-13 ID:SGTC_2794|Compound:7785382|FD-Score:4.32|P-value:1.38E-6 ID:SGTC_2961|Compound:9083290|FD-Score:-3.14|P-value:4.42E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-2.93|P-value:9.53E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.18|P-value:3.70E-4

Top fitness defect scores for YGR202C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 9.75 10.3 µM 3.32E-26 NEO1-PIK1 62 9.68E-23
2 SGTC_1754 st042065 8.69 16.1 µM 3.06E-21 NEO1-PIK1 46 1.85E-18
3 SGTC_1750 st081859 8.29 2.4 µM 1.70E-19 endomembrane recycling 66 5.90E-17
4 SGTC_16 erodoxin 6.73 22.8 µM 1.69E-13 unfolded protein response 21 8.76E-12
5 SGTC_2688 kanamycin b 6.55 96.7 µM 6.61E-13 calcium & mitochondrial duress 127 2.83E-11
6 SGTC_987 1497-1013 4.36 404.0 µM 1.09E-6 8 6.47E-6
7 SGTC_2794 7785382 4.32 27.3 µM 1.38E-6 23 7.93E-6
8 SGTC_535 2293-4129 4.03 48.2 µM 6.00E-6 NEO1 56 2.82E-5
9 SGTC_439 amiodarone 4.01 34.4 µM 6.52E-6 NEO1-PIK1 94 3.03E-5
10 SGTC_8 0354-0001 3.73 70.0 µM 2.51E-5 86 9.68E-5
11 SGTC_699 0375-0592 3.46 111.0 µM 8.09E-5 iron homeostasis 39 2.66E-4
12 SGTC_649 1123-0311 3.45 367.0 µM 8.75E-5 endomembrane recycling 101 2.84E-4
13 SGTC_1803 st050068 3.31 52.4 µM 1.58E-4 NEO1-PIK1 233 4.74E-4
14 SGTC_2491 paeonol 3.28 100.0 µM 1.76E-4 17 5.19E-4
15 SGTC_207 4185-0010 3.28 4.5 µM 1.78E-4 endomembrane recycling 128 5.24E-4
16 SGTC_988 1497-1057 3.27 103.0 µM 1.80E-4 27 5.31E-4
17 SGTC_986 1493-0483 3.23 84.0 µM 2.14E-4 35 6.15E-4
18 SGTC_2021 4019055 3.20 173.0 µM 2.46E-4 endomembrane recycling 194 6.94E-4
19 SGTC_1338 1486-1223 3.19 76.0 µM 2.52E-4 46 7.08E-4
20 SGTC_705 1124-0212 3.16 310.0 µM 2.80E-4 44 7.76E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.405 1.21E-132 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
0.388 5.66E-121 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.354 1.33E-99 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.343 5.27E-93 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.329 1.53E-85 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.316 1.19E-78 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.310 1.03E-75 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.310 1.08E-75 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.309 2.56E-75 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.308 9.35E-75 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.306 1.37E-73 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.306 1.26E-73 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.303 2.95E-72 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.303 3.70E-72 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.301 5.58E-71 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p