YGR204W / ADE3

Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR204W / ADE3

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.80|P-value:1.49E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.63|P-value:2.67E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:4.84|P-value:2.21E-6 ID:SGTC_25|Compound:0118-0223|FD-Score:3.40|P-value:5.85E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.40|P-value:5.79E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:4.29|P-value:2.28E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.39|P-value:9.94E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:3.98|P-value:7.71E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:39.40|P-value:5.10E-297 ID:SGTC_309|Compound:k408-0051|FD-Score:3.27|P-value:8.86E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.31|P-value:7.91E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.74|P-value:3.18E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.67|P-value:2.31E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-4.06|P-value:1.02E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:-3.98|P-value:1.35E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:-3.74|P-value:3.20E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.87|P-value:1.14E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.47|P-value:4.69E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:5.18|P-value:4.62E-7 ID:SGTC_800|Compound:3448-7362|FD-Score:4.21|P-value:3.08E-5 ID:SGTC_856|Compound:0433-0016|FD-Score:3.28|P-value:8.68E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:-3.51|P-value:6.81E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.60|P-value:3.00E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.98|P-value:1.15E-6 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.48|P-value:4.41E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.30|P-value:2.19E-5 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:-3.49|P-value:7.24E-4 ID:SGTC_1766|Compound:st045414|FD-Score:-4.00|P-value:1.26E-4 ID:SGTC_1811|Compound:st050069|FD-Score:-3.82|P-value:2.44E-4 ID:SGTC_1812|Compound:st052057|FD-Score:4.65|P-value:5.01E-6 ID:SGTC_2172|Compound:5754775|FD-Score:4.00|P-value:7.16E-5 ID:SGTC_2184|Compound:6075246|FD-Score:-3.44|P-value:8.44E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.40|P-value:5.85E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.58|P-value:5.40E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.45|P-value:5.02E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-3.45|P-value:8.30E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.58|P-value:3.23E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.52|P-value:3.95E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.84|P-value:1.25E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:5.82|P-value:1.85E-8 ID:SGTC_2537|Compound:larixol|FD-Score:4.72|P-value:3.83E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.53|P-value:3.82E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-4.23|P-value:5.28E-5 ID:SGTC_2709|Compound:st078560|FD-Score:-3.48|P-value:7.60E-4 ID:SGTC_2828|Compound:7998861|FD-Score:5.50|P-value:9.58E-8 ID:SGTC_2851|Compound:9015922|FD-Score:3.69|P-value:2.14E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.47|P-value:7.74E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.50|P-value:4.22E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-3.58|P-value:5.43E-4 ID:SGTC_3160|Compound:9100216|FD-Score:4.01|P-value:6.72E-5 ID:SGTC_3173|Compound:9105693|FD-Score:3.79|P-value:1.53E-4 ID:SGTC_3187|Compound:9109394|FD-Score:-3.47|P-value:7.69E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-5.08|P-value:1.54E-6 ID:SGTC_3244|Compound:9134489|FD-Score:15.00|P-value:2.80E-45 ID:SGTC_7|Compound:0192-1582|FD-Score:3.80|P-value:1.49E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:3.63|P-value:2.67E-4 ID:SGTC_14|Compound:0354-0009|FD-Score:4.84|P-value:2.21E-6 ID:SGTC_25|Compound:0118-0223|FD-Score:3.40|P-value:5.85E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.40|P-value:5.79E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:4.29|P-value:2.28E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:-3.39|P-value:9.94E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:3.98|P-value:7.71E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:39.40|P-value:5.10E-297 ID:SGTC_309|Compound:k408-0051|FD-Score:3.27|P-value:8.86E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.31|P-value:7.91E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.74|P-value:3.18E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.67|P-value:2.31E-4 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-4.06|P-value:1.02E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:-3.98|P-value:1.35E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:-3.74|P-value:3.20E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:3.87|P-value:1.14E-4 ID:SGTC_735|Compound:3076-0345|FD-Score:3.47|P-value:4.69E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:5.18|P-value:4.62E-7 ID:SGTC_800|Compound:3448-7362|FD-Score:4.21|P-value:3.08E-5 ID:SGTC_856|Compound:0433-0016|FD-Score:3.28|P-value:8.68E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:-3.51|P-value:6.81E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.60|P-value:3.00E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.98|P-value:1.15E-6 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.48|P-value:4.41E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.30|P-value:2.19E-5 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:-3.49|P-value:7.24E-4 ID:SGTC_1766|Compound:st045414|FD-Score:-4.00|P-value:1.26E-4 ID:SGTC_1811|Compound:st050069|FD-Score:-3.82|P-value:2.44E-4 ID:SGTC_1812|Compound:st052057|FD-Score:4.65|P-value:5.01E-6 ID:SGTC_2172|Compound:5754775|FD-Score:4.00|P-value:7.16E-5 ID:SGTC_2184|Compound:6075246|FD-Score:-3.44|P-value:8.44E-4 ID:SGTC_2265|Compound:7929201|FD-Score:3.40|P-value:5.85E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.58|P-value:5.40E-4 ID:SGTC_2316|Compound:5844804|FD-Score:3.45|P-value:5.02E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-3.45|P-value:8.30E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.58|P-value:3.23E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.52|P-value:3.95E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.84|P-value:1.25E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:5.82|P-value:1.85E-8 ID:SGTC_2537|Compound:larixol|FD-Score:4.72|P-value:3.83E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.53|P-value:3.82E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:-4.23|P-value:5.28E-5 ID:SGTC_2709|Compound:st078560|FD-Score:-3.48|P-value:7.60E-4 ID:SGTC_2828|Compound:7998861|FD-Score:5.50|P-value:9.58E-8 ID:SGTC_2851|Compound:9015922|FD-Score:3.69|P-value:2.14E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.47|P-value:7.74E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.74|P-value:1.84E-4 ID:SGTC_3050|Compound:9093513|FD-Score:3.50|P-value:4.22E-4 ID:SGTC_3073|Compound:9114972|FD-Score:-3.58|P-value:5.43E-4 ID:SGTC_3160|Compound:9100216|FD-Score:4.01|P-value:6.72E-5 ID:SGTC_3173|Compound:9105693|FD-Score:3.79|P-value:1.53E-4 ID:SGTC_3187|Compound:9109394|FD-Score:-3.47|P-value:7.69E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-5.08|P-value:1.54E-6 ID:SGTC_3244|Compound:9134489|FD-Score:15.00|P-value:2.80E-45

Top fitness defect scores for YGR204W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_123 0083-0120 39.40 66.7 µM 5.10E-297 1 0
2 SGTC_3244 9134489 15.00 49.5 µM 2.80E-45 2 3.62E-51
3 SGTC_2531 methyl deoxycholate 5.82 70.1 µM 1.85E-8 6 2.96E-9
4 SGTC_2828 7998861 5.50 71.4 µM 9.58E-8 5 1.89E-8
5 SGTC_782 0083-0095 5.18 30.5 µM 4.62E-7 10 1.11E-7
6 SGTC_1155 4088-0394 4.98 48.2 µM 1.15E-6 8 3.11E-7
7 SGTC_14 0354-0009 4.84 295.0 µM 2.21E-6 12 6.51E-7
8 SGTC_2537 larixol 4.72 68.9 µM 3.83E-6 11 1.21E-6
9 SGTC_1812 st052057 4.65 4.3 µM 5.01E-6 calcium & mitochondrial duress 13 1.63E-6
10 SGTC_1206 3978-0017 4.30 132.0 µM 2.19E-5 ERG2 23 8.56E-6
11 SGTC_90 0870-0863 4.29 4.6 µM 2.28E-5 38 8.95E-6
12 SGTC_800 3448-7362 4.21 155.0 µM 3.08E-5 translation 143 1.25E-5
13 SGTC_3160 9100216 4.01 49.5 µM 6.72E-5 5 3.01E-5
14 SGTC_2172 5754775 4.00 163.9 µM 7.16E-5 14 3.23E-5
15 SGTC_108 0509-0257 3.98 621.1 µM 7.71E-5 51 3.51E-5
16 SGTC_709 3807-1509 3.87 243.0 µM 1.14E-4 RSC complex & mRNA processing 14 5.45E-5
17 SGTC_2422 chlorphenesin carbamate 3.84 384.2 µM 1.25E-4 28 6.05E-5
18 SGTC_7 0192-1582 3.80 4.2 µM 1.49E-4 65 7.35E-5
19 SGTC_3173 9105693 3.79 49.5 µM 1.53E-4 23 7.55E-5
20 SGTC_1099 5-nonyloxytryptamine 3.74 2.9 µM 1.84E-4 25 9.28E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.473 1.08E-186 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.468 1.72E-182 YLR058C SHM2 hom Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.466 1.71E-180 YGR061C ADE6 hom Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.416 1.37E-140 YMR300C ADE4 hom Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.337 5.06E-90 YDR408C ADE8 hom Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.240 4.20E-45 YMR120C ADE17 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.230 2.13E-41 YKR080W MTD1 hom NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
0.193 1.44E-29 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.149 4.85E-18 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.144 5.70E-17 YMR194C-B CMC4 hom Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
0.137 1.67E-15 YGR239C PEX21 hom Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress
0.132 2.06E-14 YGR024C THG1 het tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
0.130 3.61E-14 YPR086W SUA7 het Transcription factor TFIIB, a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
0.129 5.39E-14 YLR264W RPS28B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
0.129 5.81E-14 YDL080C THI3 hom Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis