YGR261C / APL6

Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR261C / APL6

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.72|P-value:3.74E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.24|P-value:4.57E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:3.68|P-value:4.30E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:5.41|P-value:4.45E-7 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:3.74|P-value:3.56E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:6.68|P-value:6.37E-10 ID:SGTC_263|Compound:alizarin|FD-Score:4.09|P-value:1.05E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.56|P-value:4.92E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:4.33|P-value:4.35E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:7.33|P-value:1.30E-11 ID:SGTC_308|Compound:4092-1169|FD-Score:3.47|P-value:8.60E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.65|P-value:9.76E-8 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.05|P-value:9.19E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.76|P-value:7.78E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:5.05|P-value:2.24E-6 ID:SGTC_359|Compound:0081-0058|FD-Score:-14.00|P-value:3.43E-38 ID:SGTC_363|Compound:0455-0021|FD-Score:8.55|P-value:3.43E-15 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.55|P-value:2.36E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:3.52|P-value:7.19E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:7.48|P-value:4.92E-12 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.84|P-value:5.54E-8 ID:SGTC_481|Compound:bay k-8644|FD-Score:5.04|P-value:2.38E-6 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.25|P-value:9.47E-7 ID:SGTC_524|Compound:r073-0010|FD-Score:3.49|P-value:7.86E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:8.45|P-value:7.06E-15 ID:SGTC_582|Compound:0987-0078|FD-Score:3.80|P-value:2.90E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.89|P-value:2.16E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.82|P-value:2.67E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.94|P-value:1.37E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.61|P-value:5.48E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:8.09|P-value:8.75E-14 ID:SGTC_870|Compound:0573-2213|FD-Score:3.81|P-value:2.80E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.10|P-value:1.03E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.03|P-value:2.48E-6 ID:SGTC_1227|Compound:3909-8010|FD-Score:4.51|P-value:2.17E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:-4.40|P-value:2.45E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:-4.16|P-value:6.05E-5 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.66|P-value:4.62E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.90|P-value:2.04E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.94|P-value:1.76E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.88|P-value:4.81E-6 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.78|P-value:3.12E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.79|P-value:2.28E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:5.69|P-value:1.16E-7 ID:SGTC_1559|Compound:coumarin 343|FD-Score:-4.52|P-value:1.52E-5 ID:SGTC_1658|Compound:st012944|FD-Score:4.62|P-value:1.39E-5 ID:SGTC_1660|Compound:st012960|FD-Score:4.38|P-value:3.52E-5 ID:SGTC_1687|Compound:st020778|FD-Score:13.00|P-value:1.71E-32 ID:SGTC_1712|Compound:st032288|FD-Score:4.31|P-value:4.70E-5 ID:SGTC_1830|Compound:st055982|FD-Score:3.58|P-value:6.04E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:8.55|P-value:3.37E-15 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.55|P-value:2.32E-7 ID:SGTC_1953|Compound:st076800|FD-Score:4.15|P-value:8.54E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.08|P-value:1.10E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.92|P-value:3.98E-6 ID:SGTC_2077|Compound:5212015|FD-Score:5.01|P-value:2.77E-6 ID:SGTC_2144|Compound:5628481|FD-Score:3.74|P-value:3.60E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.16|P-value:8.06E-5 ID:SGTC_2275|Compound:7947463|FD-Score:3.89|P-value:2.16E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.64|P-value:4.92E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.61|P-value:5.39E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.59|P-value:1.59E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.71|P-value:3.86E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.57|P-value:6.17E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:5.44|P-value:3.82E-7 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.87|P-value:4.87E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.43|P-value:6.98E-12 ID:SGTC_2737|Compound:bleomycin|FD-Score:10.60|P-value:1.62E-22 ID:SGTC_2779|Compound:methotrexate|FD-Score:12.90|P-value:3.45E-32 ID:SGTC_2833|Compound:9001315|FD-Score:5.67|P-value:1.27E-7 ID:SGTC_2907|Compound:7721985|FD-Score:3.84|P-value:2.54E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.55|P-value:6.51E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.92|P-value:1.92E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.72|P-value:3.80E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.03|P-value:1.29E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-3.38|P-value:8.79E-4 ID:SGTC_3141|Compound:9094609|FD-Score:5.66|P-value:1.34E-7 ID:SGTC_3142|Compound:9086498|FD-Score:6.44|P-value:2.39E-9 ID:SGTC_3155|Compound:9099265|FD-Score:4.45|P-value:2.78E-5 ID:SGTC_3308|Compound:9128332|FD-Score:4.07|P-value:1.15E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.72|P-value:3.74E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.24|P-value:4.57E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:3.68|P-value:4.30E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:5.41|P-value:4.45E-7 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:3.74|P-value:3.56E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:6.68|P-value:6.37E-10 ID:SGTC_263|Compound:alizarin|FD-Score:4.09|P-value:1.05E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.56|P-value:4.92E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:4.33|P-value:4.35E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:7.33|P-value:1.30E-11 ID:SGTC_308|Compound:4092-1169|FD-Score:3.47|P-value:8.60E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.65|P-value:9.76E-8 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.05|P-value:9.19E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.76|P-value:7.78E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:5.05|P-value:2.24E-6 ID:SGTC_359|Compound:0081-0058|FD-Score:-14.00|P-value:3.43E-38 ID:SGTC_363|Compound:0455-0021|FD-Score:8.55|P-value:3.43E-15 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.55|P-value:2.36E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:3.52|P-value:7.19E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:7.48|P-value:4.92E-12 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.84|P-value:5.54E-8 ID:SGTC_481|Compound:bay k-8644|FD-Score:5.04|P-value:2.38E-6 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.25|P-value:9.47E-7 ID:SGTC_524|Compound:r073-0010|FD-Score:3.49|P-value:7.86E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:8.45|P-value:7.06E-15 ID:SGTC_582|Compound:0987-0078|FD-Score:3.80|P-value:2.90E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.89|P-value:2.16E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.82|P-value:2.67E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.94|P-value:1.37E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:3.61|P-value:5.48E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:8.09|P-value:8.75E-14 ID:SGTC_870|Compound:0573-2213|FD-Score:3.81|P-value:2.80E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:4.10|P-value:1.03E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.03|P-value:2.48E-6 ID:SGTC_1227|Compound:3909-8010|FD-Score:4.51|P-value:2.17E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:-4.40|P-value:2.45E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:-4.16|P-value:6.05E-5 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.66|P-value:4.62E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.90|P-value:2.04E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.94|P-value:1.76E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.88|P-value:4.81E-6 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.78|P-value:3.12E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.79|P-value:2.28E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:5.69|P-value:1.16E-7 ID:SGTC_1559|Compound:coumarin 343|FD-Score:-4.52|P-value:1.52E-5 ID:SGTC_1658|Compound:st012944|FD-Score:4.62|P-value:1.39E-5 ID:SGTC_1660|Compound:st012960|FD-Score:4.38|P-value:3.52E-5 ID:SGTC_1687|Compound:st020778|FD-Score:13.00|P-value:1.71E-32 ID:SGTC_1712|Compound:st032288|FD-Score:4.31|P-value:4.70E-5 ID:SGTC_1830|Compound:st055982|FD-Score:3.58|P-value:6.04E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:8.55|P-value:3.37E-15 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.55|P-value:2.32E-7 ID:SGTC_1953|Compound:st076800|FD-Score:4.15|P-value:8.54E-5 ID:SGTC_2011|Compound:4012611|FD-Score:4.08|P-value:1.10E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.92|P-value:3.98E-6 ID:SGTC_2077|Compound:5212015|FD-Score:5.01|P-value:2.77E-6 ID:SGTC_2144|Compound:5628481|FD-Score:3.74|P-value:3.60E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.16|P-value:8.06E-5 ID:SGTC_2275|Compound:7947463|FD-Score:3.89|P-value:2.16E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.64|P-value:4.92E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.61|P-value:5.39E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.59|P-value:1.59E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.71|P-value:3.86E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.57|P-value:6.17E-4 ID:SGTC_2586|Compound:curcumin|FD-Score:5.44|P-value:3.82E-7 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.87|P-value:4.87E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.43|P-value:6.98E-12 ID:SGTC_2737|Compound:bleomycin|FD-Score:10.60|P-value:1.62E-22 ID:SGTC_2779|Compound:methotrexate|FD-Score:12.90|P-value:3.45E-32 ID:SGTC_2833|Compound:9001315|FD-Score:5.67|P-value:1.27E-7 ID:SGTC_2907|Compound:7721985|FD-Score:3.84|P-value:2.54E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.55|P-value:6.51E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.92|P-value:1.92E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.72|P-value:3.80E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.03|P-value:1.29E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-3.38|P-value:8.79E-4 ID:SGTC_3141|Compound:9094609|FD-Score:5.66|P-value:1.34E-7 ID:SGTC_3142|Compound:9086498|FD-Score:6.44|P-value:2.39E-9 ID:SGTC_3155|Compound:9099265|FD-Score:4.45|P-value:2.78E-5 ID:SGTC_3308|Compound:9128332|FD-Score:4.07|P-value:1.15E-4

Top fitness defect scores for YGR261C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1687 st020778 13.00 55.4 µM 1.71E-32 30 1.16E-38
2 SGTC_2779 methotrexate 12.90 400.0 µM 3.45E-32 2 2.69E-38
3 SGTC_2737 bleomycin 10.60 30.5 nM 1.62E-22 Golgi 10 1.03E-26
4 SGTC_1873 rapamycin 8.55 2.0 nM 3.37E-15 11 5.94E-18
5 SGTC_363 0455-0021 8.55 3.1 µM 3.43E-15 azole & statin 12 6.06E-18
6 SGTC_528 3492-0019 8.45 172.0 µM 7.06E-15 calcium & mitochondrial duress 63 1.44E-17
7 SGTC_841 0251-0215 8.09 91.2 µM 8.75E-14 20 2.94E-16
8 SGTC_431 k089-0097 7.48 107.0 µM 4.92E-12 iron homeostasis 4 3.65E-14
9 SGTC_2688 kanamycin b 7.43 96.7 µM 6.98E-12 calcium & mitochondrial duress 88 5.56E-14
10 SGTC_301 4119-0100 7.33 24.5 µM 1.30E-11 NEO1 25 1.17E-13
11 SGTC_245 methotrexate 6.68 289.6 µM 6.37E-10 7 1.24E-11
12 SGTC_3142 9086498 6.44 49.5 µM 2.39E-9 iron homeostasis 4 6.02E-11
13 SGTC_445 e6 berbamine 5.84 66.0 µM 5.54E-8 NEO1-PIK1 36 2.59E-9
14 SGTC_1550 gossypolone 5.69 36.6 µM 1.16E-7 2 6.30E-9
15 SGTC_2833 9001315 5.67 45.5 µM 1.27E-7 amide catabolism 22 6.95E-9
16 SGTC_3141 9094609 5.66 49.5 µM 1.34E-7 iron homeostasis 8 7.45E-9
17 SGTC_1877 wortmannin 5.55 25.0 µM 2.32E-7 53 1.44E-8
18 SGTC_391 gliotoxin 5.55 8.3 µM 2.36E-7 31 1.46E-8
19 SGTC_2586 curcumin 5.44 80.0 µM 3.82E-7 iron homeostasis 26 2.61E-8
20 SGTC_123 0083-0120 5.41 66.7 µM 4.45E-7 24 3.13E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.445 1.61E-162 YJL024C APS3 hom Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
0.437 7.94E-157 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.419 3.34E-143 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.392 9.42E-124 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.385 6.14E-119 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.372 1.35E-110 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.319 4.80E-80 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.311 2.60E-76 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.302 7.86E-72 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.268 2.70E-56 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.256 3.31E-51 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.251 1.81E-49 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.239 7.62E-45 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.237 5.80E-44 YGL012W ERG4 hom C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
0.227 1.27E-40 YBR131W CCZ1 hom Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway