HIPHOP chemogenomics database

YHL003C / LAG1

Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YHL003C / LAG1

Click on Significant Values for Screen Details

Top fitness defect scores for YHL003C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_3212	9128307	14.70	49.5 µM	6.60E-62		2	3.13E-49
2	SGTC_3208	9114115	6.32	49.5 µM	5.14E-13		9	1.27E-10
3	SGTC_3076	9116187	5.65	49.5 µM	9.80E-11		3	8.16E-9
4	SGTC_2557	derrustone	4.56	83.8 µM	1.35E-7		19	2.52E-6
5	SGTC_1059	3448-6867	4.16	171.0 µM	1.36E-6		10	1.58E-5
6	SGTC_2640	pentamidine	4.10	10.4 µM	1.90E-6	mitochondrial processes	30	2.06E-5
7	SGTC_6	0844-0013	3.72	19.8 µM	1.36E-5		115	9.94E-5
8	SGTC_129	0088-0017	3.64	176.6 µM	2.03E-5		76	1.36E-4
9	SGTC_1381	2324-0066	3.59	62.1 µM	2.63E-5		41	1.68E-4
10	SGTC_69	0096-0274	3.57	206.7 µM	2.81E-5	mitochondrial processes	58	1.77E-4
11	SGTC_3200	9114385	3.56	49.5 µM	3.02E-5		52	1.87E-4
12	SGTC_2200	6600604	3.54	21.9 µM	3.34E-5	tubulin folding & SWR complex	61	2.03E-4
13	SGTC_73	0327-0325	3.52	16.2 µM	3.65E-5	DNA damage response	68	2.18E-4
14	SGTC_2618	avocadyne	3.51	43.4 µM	3.70E-5		46	2.20E-4
15	SGTC_1125	tnp00248	3.51	1.3 µM	3.77E-5		49	2.23E-4
16	SGTC_1315	1237-0019	3.51	3.7 µM	3.79E-5		13	2.24E-4
17	SGTC_287	k060-0039	3.50	29.6 µM	4.03E-5		18	2.36E-4
18	SGTC_2563	tomatine	3.48	8.5 µM	4.25E-5		72	2.46E-4
19	SGTC_1567	st069303	3.33	68.4 µM	8.61E-5	mitochondrial processes	41	4.32E-4
20	SGTC_2006	4011487	3.25	200.0 µM	1.25E-4	endomembrane recycling	119	5.83E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.230	2.56E-41	YPL167C	REV3	hom	Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.201	6.84E-32	YHR130C_d	YHR130C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.196	3.19E-30	YOR055W_d	YOR055W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184	6.24E-27	YML069W	POB3	het	Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
0.177	3.90E-25	YKL201C	MNN4	hom	Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.165	8.08E-22	YJR107W_p	YJR107W_p	hom	Putative lipase
0.151	1.55E-18	YKL116C	PRR1	hom	Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor
0.137	1.62E-15	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.128	8.98E-14	YKL218C	SRY1	hom	3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity
0.127	1.79E-13	YGL017W	ATE1	hom	Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.121	1.87E-12	YOR034C	AKR2	hom	Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
0.116	1.54E-11	YCR061W_p	YCR061W_p	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
0.116	1.91E-11	YGL082W_p	YGL082W_p	hom	Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
0.115	2.08E-11	YDL027C_p	YDL027C_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
0.115	2.22E-11	YLR312W-A	MRPL15	hom	Mitochondrial ribosomal protein of the large subunit