YHL003C / LAG1

Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL003C / LAG1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.72|P-value:1.36E-5 ID:SGTC_69|Compound:0096-0274|FD-Score:3.57|P-value:2.81E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:3.52|P-value:3.65E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:3.64|P-value:2.03E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.01|P-value:3.51E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:3.50|P-value:4.03E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:-4.00|P-value:3.40E-6 ID:SGTC_480|Compound:am 92016|FD-Score:2.84|P-value:6.88E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.13|P-value:2.10E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:2.87|P-value:5.94E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:2.75|P-value:9.53E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.09|P-value:2.47E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.22|P-value:1.49E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:4.16|P-value:1.36E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.62|P-value:2.38E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.33|P-value:9.09E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:-5.83|P-value:2.64E-11 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.51|P-value:3.77E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.81|P-value:7.65E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.72|P-value:1.44E-5 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.51|P-value:3.79E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:-3.59|P-value:2.73E-5 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.59|P-value:2.63E-5 ID:SGTC_1567|Compound:st069303|FD-Score:3.33|P-value:8.61E-5 ID:SGTC_1696|Compound:st025591|FD-Score:-2.91|P-value:5.36E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.18|P-value:1.68E-4 ID:SGTC_1820|Compound:st053765|FD-Score:2.87|P-value:5.97E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.03|P-value:3.15E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.25|P-value:1.25E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-2.79|P-value:8.42E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.07|P-value:2.84E-4 ID:SGTC_2104|Compound:5389443|FD-Score:-2.78|P-value:8.78E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.09|P-value:2.53E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-2.89|P-value:5.89E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.54|P-value:3.34E-5 ID:SGTC_2241|Compound:6772625|FD-Score:3.14|P-value:1.99E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-2.78|P-value:8.87E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.56|P-value:1.35E-7 ID:SGTC_2563|Compound:tomatine|FD-Score:3.48|P-value:4.25E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:-2.79|P-value:8.51E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.23|P-value:1.36E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.51|P-value:3.70E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.22|P-value:1.39E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.10|P-value:1.90E-6 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.16|P-value:1.82E-4 ID:SGTC_2802|Compound:7925815|FD-Score:2.83|P-value:7.19E-4 ID:SGTC_2823|Compound:7987624|FD-Score:2.86|P-value:6.25E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.14|P-value:2.05E-4 ID:SGTC_2998|Compound:9066762|FD-Score:3.02|P-value:3.33E-4 ID:SGTC_3029|Compound:9088683|FD-Score:2.87|P-value:6.10E-4 ID:SGTC_3076|Compound:9116187|FD-Score:5.65|P-value:9.80E-11 ID:SGTC_3200|Compound:9114385|FD-Score:3.56|P-value:3.02E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.03|P-value:3.17E-4 ID:SGTC_3208|Compound:9114115|FD-Score:6.32|P-value:5.14E-13 ID:SGTC_3212|Compound:9128307|FD-Score:14.70|P-value:6.60E-62 ID:SGTC_6|Compound:0844-0013|FD-Score:3.72|P-value:1.36E-5 ID:SGTC_69|Compound:0096-0274|FD-Score:3.57|P-value:2.81E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:3.52|P-value:3.65E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:3.64|P-value:2.03E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.01|P-value:3.51E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:3.50|P-value:4.03E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:-4.00|P-value:3.40E-6 ID:SGTC_480|Compound:am 92016|FD-Score:2.84|P-value:6.88E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.13|P-value:2.10E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:2.87|P-value:5.94E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:2.75|P-value:9.53E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:3.09|P-value:2.47E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.22|P-value:1.49E-4 ID:SGTC_1059|Compound:3448-6867|FD-Score:4.16|P-value:1.36E-6 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.62|P-value:2.38E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.33|P-value:9.09E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:-5.83|P-value:2.64E-11 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.51|P-value:3.77E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:2.81|P-value:7.65E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.72|P-value:1.44E-5 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.51|P-value:3.79E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:-3.59|P-value:2.73E-5 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.59|P-value:2.63E-5 ID:SGTC_1567|Compound:st069303|FD-Score:3.33|P-value:8.61E-5 ID:SGTC_1696|Compound:st025591|FD-Score:-2.91|P-value:5.36E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.18|P-value:1.68E-4 ID:SGTC_1820|Compound:st053765|FD-Score:2.87|P-value:5.97E-4 ID:SGTC_2001|Compound:4023502|FD-Score:3.03|P-value:3.15E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.25|P-value:1.25E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-2.79|P-value:8.42E-4 ID:SGTC_2053|Compound:5265679|FD-Score:-3.07|P-value:2.84E-4 ID:SGTC_2104|Compound:5389443|FD-Score:-2.78|P-value:8.78E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.09|P-value:2.53E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-2.89|P-value:5.89E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.54|P-value:3.34E-5 ID:SGTC_2241|Compound:6772625|FD-Score:3.14|P-value:1.99E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-2.78|P-value:8.87E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:4.56|P-value:1.35E-7 ID:SGTC_2563|Compound:tomatine|FD-Score:3.48|P-value:4.25E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:-2.79|P-value:8.51E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.23|P-value:1.36E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.51|P-value:3.70E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.22|P-value:1.39E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.10|P-value:1.90E-6 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.16|P-value:1.82E-4 ID:SGTC_2802|Compound:7925815|FD-Score:2.83|P-value:7.19E-4 ID:SGTC_2823|Compound:7987624|FD-Score:2.86|P-value:6.25E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.14|P-value:2.05E-4 ID:SGTC_2998|Compound:9066762|FD-Score:3.02|P-value:3.33E-4 ID:SGTC_3029|Compound:9088683|FD-Score:2.87|P-value:6.10E-4 ID:SGTC_3076|Compound:9116187|FD-Score:5.65|P-value:9.80E-11 ID:SGTC_3200|Compound:9114385|FD-Score:3.56|P-value:3.02E-5 ID:SGTC_3205|Compound:9113483|FD-Score:3.03|P-value:3.17E-4 ID:SGTC_3208|Compound:9114115|FD-Score:6.32|P-value:5.14E-13 ID:SGTC_3212|Compound:9128307|FD-Score:14.70|P-value:6.60E-62

Top fitness defect scores for YHL003C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3212 9128307 14.70 49.5 µM 6.60E-62 2 3.13E-49
2 SGTC_3208 9114115 6.32 49.5 µM 5.14E-13 9 1.27E-10
3 SGTC_3076 9116187 5.65 49.5 µM 9.80E-11 3 8.16E-9
4 SGTC_2557 derrustone 4.56 83.8 µM 1.35E-7 19 2.52E-6
5 SGTC_1059 3448-6867 4.16 171.0 µM 1.36E-6 10 1.58E-5
6 SGTC_2640 pentamidine 4.10 10.4 µM 1.90E-6 mitochondrial processes 30 2.06E-5
7 SGTC_6 0844-0013 3.72 19.8 µM 1.36E-5 115 9.94E-5
8 SGTC_129 0088-0017 3.64 176.6 µM 2.03E-5 76 1.36E-4
9 SGTC_1381 2324-0066 3.59 62.1 µM 2.63E-5 41 1.68E-4
10 SGTC_69 0096-0274 3.57 206.7 µM 2.81E-5 mitochondrial processes 58 1.77E-4
11 SGTC_3200 9114385 3.56 49.5 µM 3.02E-5 52 1.87E-4
12 SGTC_2200 6600604 3.54 21.9 µM 3.34E-5 tubulin folding & SWR complex 61 2.03E-4
13 SGTC_73 0327-0325 3.52 16.2 µM 3.65E-5 DNA damage response 68 2.18E-4
14 SGTC_2618 avocadyne 3.51 43.4 µM 3.70E-5 46 2.20E-4
15 SGTC_1125 tnp00248 3.51 1.3 µM 3.77E-5 49 2.23E-4
16 SGTC_1315 1237-0019 3.51 3.7 µM 3.79E-5 13 2.24E-4
17 SGTC_287 k060-0039 3.50 29.6 µM 4.03E-5 18 2.36E-4
18 SGTC_2563 tomatine 3.48 8.5 µM 4.25E-5 72 2.46E-4
19 SGTC_1567 st069303 3.33 68.4 µM 8.61E-5 mitochondrial processes 41 4.32E-4
20 SGTC_2006 4011487 3.25 200.0 µM 1.25E-4 endomembrane recycling 119 5.83E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.230 2.56E-41 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.201 6.84E-32 YHR130C_d YHR130C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.196 3.19E-30 YOR055W_d YOR055W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184 6.24E-27 YML069W POB3 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
0.177 3.90E-25 YKL201C MNN4 hom Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
0.165 8.08E-22 YJR107W_p YJR107W_p hom Putative lipase
0.151 1.55E-18 YKL116C PRR1 hom Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor
0.137 1.62E-15 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.128 8.98E-14 YKL218C SRY1 hom 3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity
0.127 1.79E-13 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.121 1.87E-12 YOR034C AKR2 hom Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
0.116 1.54E-11 YCR061W_p YCR061W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
0.116 1.91E-11 YGL082W_p YGL082W_p hom Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
0.115 2.08E-11 YDL027C_p YDL027C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene
0.115 2.22E-11 YLR312W-A MRPL15 hom Mitochondrial ribosomal protein of the large subunit