YHL014C / YLF2

Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL014C / YLF2

Click on Significant Values for Screen Details ID:SGTC_129|Compound:0088-0017|FD-Score:-3.83|P-value:4.94E-5 ID:SGTC_135|Compound:0933-0004|FD-Score:3.33|P-value:3.78E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.15|P-value:7.20E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:5.49|P-value:1.33E-8 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.87|P-value:4.22E-5 ID:SGTC_604|Compound:1000-0114|FD-Score:4.05|P-value:2.07E-5 ID:SGTC_628|Compound:1636-0253|FD-Score:4.02|P-value:2.34E-5 ID:SGTC_638|Compound:3970-0837|FD-Score:3.48|P-value:2.16E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:5.01|P-value:1.88E-7 ID:SGTC_784|Compound:4579-0127|FD-Score:3.43|P-value:2.62E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:3.77|P-value:6.87E-5 ID:SGTC_803|Compound:4488-1585|FD-Score:3.74|P-value:7.59E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.14|P-value:7.02E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:4.62|P-value:1.42E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:-3.46|P-value:2.15E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.08|P-value:9.14E-4 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.91|P-value:3.80E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.36|P-value:3.40E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.80|P-value:6.01E-5 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:4.24|P-value:8.95E-6 ID:SGTC_1745|Compound:st081495|FD-Score:-3.34|P-value:3.38E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.71|P-value:9.09E-7 ID:SGTC_1763|Compound:st044482|FD-Score:-3.56|P-value:1.45E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.46|P-value:2.28E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.58|P-value:1.45E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.43|P-value:3.61E-6 ID:SGTC_2023|Compound:5144124|FD-Score:-3.67|P-value:9.55E-5 ID:SGTC_2207|Compound:7258803|FD-Score:3.64|P-value:1.17E-4 ID:SGTC_2291|Compound:7917025|FD-Score:-3.92|P-value:3.39E-5 ID:SGTC_2378|Compound:9074213|FD-Score:3.29|P-value:4.31E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.57|P-value:1.51E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.59|P-value:1.37E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.25|P-value:4.99E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-3.83|P-value:4.94E-5 ID:SGTC_135|Compound:0933-0004|FD-Score:3.33|P-value:3.78E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:3.15|P-value:7.20E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:5.49|P-value:1.33E-8 ID:SGTC_404|Compound:fumagillin|FD-Score:-3.87|P-value:4.22E-5 ID:SGTC_604|Compound:1000-0114|FD-Score:4.05|P-value:2.07E-5 ID:SGTC_628|Compound:1636-0253|FD-Score:4.02|P-value:2.34E-5 ID:SGTC_638|Compound:3970-0837|FD-Score:3.48|P-value:2.16E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:5.01|P-value:1.88E-7 ID:SGTC_784|Compound:4579-0127|FD-Score:3.43|P-value:2.62E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:3.77|P-value:6.87E-5 ID:SGTC_803|Compound:4488-1585|FD-Score:3.74|P-value:7.59E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:-3.14|P-value:7.02E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:4.62|P-value:1.42E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:-3.46|P-value:2.15E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.08|P-value:9.14E-4 ID:SGTC_1191|Compound:1487-1166|FD-Score:3.91|P-value:3.80E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.36|P-value:3.40E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.80|P-value:6.01E-5 ID:SGTC_1508|Compound:1080-0625|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:4.24|P-value:8.95E-6 ID:SGTC_1745|Compound:st081495|FD-Score:-3.34|P-value:3.38E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.71|P-value:9.09E-7 ID:SGTC_1763|Compound:st044482|FD-Score:-3.56|P-value:1.45E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.46|P-value:2.28E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.58|P-value:1.45E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.43|P-value:3.61E-6 ID:SGTC_2023|Compound:5144124|FD-Score:-3.67|P-value:9.55E-5 ID:SGTC_2207|Compound:7258803|FD-Score:3.64|P-value:1.17E-4 ID:SGTC_2291|Compound:7917025|FD-Score:-3.92|P-value:3.39E-5 ID:SGTC_2378|Compound:9074213|FD-Score:3.29|P-value:4.31E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.57|P-value:1.51E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.59|P-value:1.37E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.25|P-value:4.99E-4

Top fitness defect scores for YHL014C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_154 3389-1638 5.49 511.1 µM 1.33E-8 4 2.01E-8
2 SGTC_769 0302-0231 5.01 76.0 µM 1.88E-7 16 2.65E-7
3 SGTC_1754 st042065 4.71 16.1 µM 9.09E-7 NEO1-PIK1 141 1.23E-6
4 SGTC_1061 3448-9325 4.62 110.0 µM 1.42E-6 13 1.89E-6
5 SGTC_2013 4024628 4.43 200.0 µM 3.61E-6 32 4.71E-6
6 SGTC_1528 arabinosylhypoxanthine 4.24 74.6 µM 8.95E-6 15 1.14E-5
7 SGTC_604 1000-0114 4.05 314.0 µM 2.07E-5 17 2.58E-5
8 SGTC_628 1636-0253 4.02 16.0 µM 2.34E-5 sphingolipid biosynthesis & PDR1 32 2.90E-5
9 SGTC_1191 1487-1166 3.91 10.6 µM 3.80E-5 azole & statin 33 4.66E-5
10 SGTC_1410 3909-8106 3.80 77.9 µM 6.01E-5 13 7.28E-5
11 SGTC_797 k781-0471 3.77 270.0 µM 6.87E-5 RPP1 & pyrimidine depletion 26 8.30E-5
12 SGTC_803 4488-1585 3.74 147.0 µM 7.59E-5 14 9.15E-5
13 SGTC_2207 7258803 3.64 200.0 µM 1.17E-4 10 1.39E-4
14 SGTC_2789 5281112 3.59 71.4 µM 1.37E-4 52 1.63E-4
15 SGTC_1508 1080-0625 3.59 72.9 µM 1.38E-4 30 1.64E-4
16 SGTC_1803 st050068 3.58 52.4 µM 1.45E-4 NEO1-PIK1 209 1.72E-4
17 SGTC_2411 st077133 3.57 39.9 µM 1.51E-4 RNA pol III & RNase P/MRP 43 1.79E-4
18 SGTC_638 3970-0837 3.48 4.1 µM 2.16E-4 56 2.53E-4
19 SGTC_1801 st050089 3.46 28.4 µM 2.28E-4 NEO1-PIK1 150 2.67E-4
20 SGTC_784 4579-0127 3.43 173.0 µM 2.62E-4 39 3.06E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 8.06E-18 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.141 2.12E-16 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.130 3.49E-14 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.122 1.17E-12 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.121 2.43E-12 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
0.120 2.56E-12 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.120 2.68E-12 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.119 4.25E-12 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
0.118 6.13E-12 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.118 7.05E-12 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.117 1.28E-11 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.117 1.28E-11 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.115 2.19E-11 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.115 2.40E-11 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.114 3.21E-11 YHL009C YAP3 hom Basic leucine zipper (bZIP) transcription factor