YHL029C_p / OCA5

Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL029C / OCA5

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:5.93|P-value:1.05E-7 ID:SGTC_8|Compound:0354-0001|FD-Score:8.09|P-value:7.28E-13 ID:SGTC_18|Compound:0111-0177|FD-Score:7.26|P-value:1.02E-10 ID:SGTC_31|Compound:0508-1974|FD-Score:4.82|P-value:1.21E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:6.22|P-value:2.54E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.05|P-value:2.05E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:5.16|P-value:3.03E-6 ID:SGTC_87|Compound:0100-0052|FD-Score:6.41|P-value:9.89E-9 ID:SGTC_130|Compound:0136-0219|FD-Score:-5.30|P-value:1.93E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:-4.85|P-value:1.15E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.93|P-value:3.07E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.78|P-value:2.12E-7 ID:SGTC_252|Compound:miconazole|FD-Score:4.16|P-value:1.32E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.01|P-value:2.18E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.89|P-value:3.29E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.87|P-value:3.41E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.58|P-value:8.53E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:4.14|P-value:1.41E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:-3.99|P-value:2.53E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:4.32|P-value:7.77E-5 ID:SGTC_995|Compound:3931-2021|FD-Score:4.72|P-value:1.76E-5 ID:SGTC_1184|Compound:1269-1718|FD-Score:5.13|P-value:3.56E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:5.23|P-value:2.30E-6 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.57|P-value:8.75E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.91|P-value:3.09E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.60|P-value:7.99E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.57|P-value:8.80E-4 ID:SGTC_1801|Compound:st050089|FD-Score:4.42|P-value:5.31E-5 ID:SGTC_1850|Compound:st056238|FD-Score:4.96|P-value:6.89E-6 ID:SGTC_1851|Compound:st056236|FD-Score:-3.90|P-value:3.38E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.57|P-value:9.34E-4 ID:SGTC_1971|Compound:st069429|FD-Score:-4.09|P-value:1.84E-4 ID:SGTC_2017|Compound:5132927|FD-Score:6.07|P-value:5.26E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.09|P-value:1.66E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.29|P-value:8.46E-5 ID:SGTC_2387|Compound:9077560|FD-Score:3.98|P-value:2.39E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.00|P-value:2.24E-4 ID:SGTC_2643|Compound:anethole|FD-Score:-3.96|P-value:2.76E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:6.64|P-value:3.10E-9 ID:SGTC_2649|Compound:anisomycin|FD-Score:7.88|P-value:2.70E-12 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.49|P-value:7.59E-7 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.90|P-value:1.17E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.55|P-value:3.40E-5 ID:SGTC_2691|Compound:st077356|FD-Score:3.66|P-value:6.75E-4 ID:SGTC_2803|Compound:7707263|FD-Score:5.75|P-value:2.40E-7 ID:SGTC_2851|Compound:9015922|FD-Score:5.01|P-value:5.74E-6 ID:SGTC_2888|Compound:9058547|FD-Score:3.67|P-value:6.56E-4 ID:SGTC_2896|Compound:9052656|FD-Score:5.81|P-value:1.81E-7 ID:SGTC_2988|Compound:9073640|FD-Score:-4.21|P-value:1.23E-4 ID:SGTC_2998|Compound:9066762|FD-Score:5.80|P-value:1.92E-7 ID:SGTC_3046|Compound:9092212|FD-Score:3.53|P-value:9.84E-4 ID:SGTC_3260|Compound:9137109|FD-Score:-3.93|P-value:3.10E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.81|P-value:4.24E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:5.93|P-value:1.05E-7 ID:SGTC_8|Compound:0354-0001|FD-Score:8.09|P-value:7.28E-13 ID:SGTC_18|Compound:0111-0177|FD-Score:7.26|P-value:1.02E-10 ID:SGTC_31|Compound:0508-1974|FD-Score:4.82|P-value:1.21E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:6.22|P-value:2.54E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.05|P-value:2.05E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:5.16|P-value:3.03E-6 ID:SGTC_87|Compound:0100-0052|FD-Score:6.41|P-value:9.89E-9 ID:SGTC_130|Compound:0136-0219|FD-Score:-5.30|P-value:1.93E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:-4.85|P-value:1.15E-5 ID:SGTC_174|Compound:3553-0389|FD-Score:-3.93|P-value:3.07E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.78|P-value:2.12E-7 ID:SGTC_252|Compound:miconazole|FD-Score:4.16|P-value:1.32E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.01|P-value:2.18E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.89|P-value:3.29E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.87|P-value:3.41E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.58|P-value:8.53E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:4.14|P-value:1.41E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:-3.99|P-value:2.53E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:4.32|P-value:7.77E-5 ID:SGTC_995|Compound:3931-2021|FD-Score:4.72|P-value:1.76E-5 ID:SGTC_1184|Compound:1269-1718|FD-Score:5.13|P-value:3.56E-6 ID:SGTC_1226|Compound:3909-7979|FD-Score:5.23|P-value:2.30E-6 ID:SGTC_1442|Compound:4112-3290|FD-Score:3.57|P-value:8.75E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:3.91|P-value:3.09E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.60|P-value:7.99E-4 ID:SGTC_1800|Compound:st050079|FD-Score:3.57|P-value:8.80E-4 ID:SGTC_1801|Compound:st050089|FD-Score:4.42|P-value:5.31E-5 ID:SGTC_1850|Compound:st056238|FD-Score:4.96|P-value:6.89E-6 ID:SGTC_1851|Compound:st056236|FD-Score:-3.90|P-value:3.38E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.57|P-value:9.34E-4 ID:SGTC_1971|Compound:st069429|FD-Score:-4.09|P-value:1.84E-4 ID:SGTC_2017|Compound:5132927|FD-Score:6.07|P-value:5.26E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.09|P-value:1.66E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.29|P-value:8.46E-5 ID:SGTC_2387|Compound:9077560|FD-Score:3.98|P-value:2.39E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.00|P-value:2.24E-4 ID:SGTC_2643|Compound:anethole|FD-Score:-3.96|P-value:2.76E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:6.64|P-value:3.10E-9 ID:SGTC_2649|Compound:anisomycin|FD-Score:7.88|P-value:2.70E-12 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.49|P-value:7.59E-7 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.90|P-value:1.17E-7 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.55|P-value:3.40E-5 ID:SGTC_2691|Compound:st077356|FD-Score:3.66|P-value:6.75E-4 ID:SGTC_2803|Compound:7707263|FD-Score:5.75|P-value:2.40E-7 ID:SGTC_2851|Compound:9015922|FD-Score:5.01|P-value:5.74E-6 ID:SGTC_2888|Compound:9058547|FD-Score:3.67|P-value:6.56E-4 ID:SGTC_2896|Compound:9052656|FD-Score:5.81|P-value:1.81E-7 ID:SGTC_2988|Compound:9073640|FD-Score:-4.21|P-value:1.23E-4 ID:SGTC_2998|Compound:9066762|FD-Score:5.80|P-value:1.92E-7 ID:SGTC_3046|Compound:9092212|FD-Score:3.53|P-value:9.84E-4 ID:SGTC_3260|Compound:9137109|FD-Score:-3.93|P-value:3.10E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.81|P-value:4.24E-4

Top fitness defect scores for YHL029C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_8 0354-0001 8.09 70.0 µM 7.28E-13 9 3.05E-16
2 SGTC_2649 anisomycin 7.88 9.6 µM 2.70E-12 1 1.68E-15
3 SGTC_18 0111-0177 7.26 500.0 nM 1.02E-10 10 1.90E-13
4 SGTC_2645 abamectin 6.64 100.0 µM 3.10E-9 heme requiring 5 1.60E-11
5 SGTC_87 0100-0052 6.41 35.5 µM 9.89E-9 26 7.20E-11
6 SGTC_46 0329-0187a 6.22 2.2 µM 2.54E-8 26 2.45E-10
7 SGTC_2017 5132927 6.07 60.6 µM 5.26E-8 4 6.30E-10
8 SGTC_7 0192-1582 5.93 4.2 µM 1.05E-7 25 1.54E-9
9 SGTC_2679 rapamycin 5.90 1.0 nM 1.17E-7 79 1.78E-9
10 SGTC_2896 9052656 5.81 71.4 µM 1.81E-7 endomembrane recycling 42 3.13E-9
11 SGTC_2998 9066762 5.80 71.4 µM 1.92E-7 RPP1 & pyrimidine depletion 3 3.37E-9
12 SGTC_221 trifluoperazine 5.78 10.3 µM 2.12E-7 NEO1-PIK1 127 3.84E-9
13 SGTC_2803 7707263 5.75 71.4 µM 2.40E-7 7 4.52E-9
14 SGTC_2651 1327-0036 5.49 5.0 µM 7.59E-7 89 2.01E-8
15 SGTC_1226 3909-7979 5.23 35.4 µM 2.30E-6 19 8.42E-8
16 SGTC_59 0330-0160 5.16 1.3 µM 3.03E-6 55 1.20E-7
17 SGTC_1184 1269-1718 5.13 139.0 µM 3.56E-6 10 1.48E-7
18 SGTC_2851 9015922 5.01 71.4 µM 5.74E-6 15 2.75E-7
19 SGTC_1850 st056238 4.96 17.0 µM 6.89E-6 RPP1 & pyrimidine depletion 9 3.48E-7
20 SGTC_31 0508-1974 4.82 57.2 µM 1.21E-5 9 7.19E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.278 1.84E-60 YNL056W OCA2 hom Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
0.273 1.42E-58 YCR095C_p OCA4_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.269 6.66E-57 YNL032W SIW14 hom Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
0.257 9.79E-52 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
0.233 1.43E-42 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.213 1.36E-35 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.193 1.28E-29 YLR451W LEU3 hom Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
0.179 1.20E-25 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.178 2.08E-25 YDR067C_p OCA6_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
0.171 2.61E-23 YPL077C_p YPL077C_p hom Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.170 4.99E-23 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.168 1.63E-22 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.166 4.39E-22 YOR274W MOD5 hom Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
0.162 3.77E-21 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.158 4.00E-20 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p