HIPHOP chemogenomics database

YHR020W

Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

Zygosity: Heterozygous strain

fixedexpanded
Profile for YHR020W / YHR020W

Click on Significant Values for Screen Details

Top fitness defect scores for YHR020W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_821	1114-0266	7.23	29.2 µM	1.11E-15		1	2.38E-13	2.42
2	SGTC_2511	berberine	6.31	10.5 µM	2.28E-12	60S ribosome export	4	1.38E-10	0.91
3	SGTC_145	3737-0113	6.30	38.4 µM	2.48E-12	DNA intercalators	15	1.48E-10	0.12
4	SGTC_2016	5121758	5.87	140.0 µM	6.06E-11	redox potentiating	6	2.14E-9	0.17
5	SGTC_126	0417-1624	5.33	672.5 µM	2.60E-9	DNA intercalators	6	4.96E-8	0.10
6	SGTC_144	4161-2749	4.75	112.4 µM	9.86E-8	DNA intercalators	8	1.04E-6	0.23
7	SGTC_1269	0831-0796	4.36	3.0 µM	8.89E-7	60S ribosome export	3	6.55E-6	0.11
8	SGTC_113	0099-0221	4.16	2.5 µM	2.58E-6		3	1.60E-5	0.36
9	SGTC_2379	9035213	4.16	200.0 µM	2.59E-6		4	1.61E-5	0.43
10	SGTC_3158	9100007	3.96	49.5 µM	7.09E-6		5	3.73E-5	0.10
11	SGTC_2522	biochanin a	3.90	4.9 µM	9.42E-6	RNA pol III & RNase P/MRP	12	4.74E-5	0.73
12	SGTC_918	glabranin	3.81	770.7 nM	1.50E-5		9	6.99E-5	0.14
13	SGTC_2640	pentamidine	3.78	10.4 µM	1.74E-5	mitochondrial processes	19	7.93E-5	0.01
14	SGTC_522	1273-0010	3.72	11.6 µM	2.32E-5	60S ribosome export	6	1.01E-4	0.08
15	SGTC_3298	9116858	3.54	71.4 µM	5.16E-5		6	1.98E-4	0.04
16	SGTC_2520	obtusaquinone	3.47	4.7 µM	7.11E-5		10	2.59E-4	0.10
17	SGTC_143	4032-1194	3.46	15.7 µM	7.41E-5		29	2.68E-4	0.02
18	SGTC_1057	3448-5381	3.19	134.0 µM	2.36E-4	calcium & mitochondrial duress	23	7.11E-4	0.00
19	SGTC_1578	josamycin	3.15	24.2 µM	2.78E-4		12	8.17E-4	0.05
20	SGTC_325	2810-4341	3.07	181.0 µM	3.80E-4	amide catabolism	14	0.00106	0.17

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.287	2.03E-64	YDR401W_d	YDR401W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.236	9.98E-44	YDR054C	CDC34	het	Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
0.203	1.59E-32	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.197	1.05E-30	YEL017C-A	PMP2	hom	Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.197	1.27E-30	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.195	4.37E-30	YEL064C	AVT2	hom	Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.189	1.96E-28	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187	8.56E-28	YFL022C	FRS2	het	Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.179	1.40E-25	YNL124W	NAF1	het	RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.173	6.71E-24	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.173	7.49E-24	YGL002W	ERP6	hom	Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.159	1.96E-20	YOR077W	RTS2	het	Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.155	1.55E-19	YMR262W_p	YMR262W_p	hom	Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.151	1.45E-18	YMR030W	RSF1	hom	Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.150	3.08E-18	YNL264C	PDR17	hom	Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition