HIPHOP chemogenomics database

YHR030C / SLT2

Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR030C / SLT2

Click on Significant Values for Screen Details

Top fitness defect scores for YHR030C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_663	1486-0733	38.60	13.9 µM	6.35E-159	cell wall signaling	1	0
2	SGTC_2776	terbinafine	35.30	19.9 µM	1.78E-133	cell wall signaling	1	3.17E-273
3	SGTC_2912	7964298	32.70	10.9 µM	4.37E-115	cell wall	2	2.64E-235
4	SGTC_500	h-89	28.10	112.0 µM	3.83E-85	cell wall signaling	1	1.44E-173
5	SGTC_422	cantharidin	27.80	155.0 µM	1.84E-83	cell wall signaling	2	4.21E-170
6	SGTC_2924	9010168	26.80	62.8 µM	1.43E-77	cell wall signaling	1	5.85E-158
7	SGTC_369	0957-0393	25.50	4.5 µM	1.24E-70	cell wall signaling	1	1.17E-143
8	SGTC_360	0957-0399	24.60	1.0 µM	5.28E-66	cell wall	1	4.08E-134
9	SGTC_241	5hr heat shock (37°C) + cantharidin	21.60	155.4 µM	1.96E-51	cell wall signaling	4	4.31E-104
10	SGTC_2007	4011539	20.70	71.8 µM	4.31E-47	cell wall signaling	3	3.83E-95
11	SGTC_2210	7115885	20.50	190.4 µM	2.45E-46	cell wall signaling	1	1.37E-93
12	SGTC_367	0335-0881	20.30	7.1 µM	1.10E-45	ubiquinone biosynthesis & proteasome	2	3.01E-92
13	SGTC_137	0438-0332	19.80	85.4 µM	2.44E-43	ubiquinone biosynthesis & proteasome	5	2.06E-87
14	SGTC_1614	st002383	19.20	48.6 µM	5.21E-41	cell wall signaling	1	1.29E-82
15	SGTC_2009	4012297	19.10	12.1 µM	1.38E-40	cell wall signaling	3	9.60E-82
16	SGTC_138	0850-0259	18.90	40.9 µM	7.86E-40	cell wall	8	3.47E-80
17	SGTC_905	2-aminofluorene	18.70	215.0 µM	4.35E-39	cell wall signaling	2	1.17E-78
18	SGTC_71	0417-1750	18.60	47.8 µM	1.71E-38	Golgi	3	1.97E-77
19	SGTC_2619	obliquin	18.40	87.9 µM	1.44E-37	cell wall	4	1.58E-75
20	SGTC_2272	7805186	18.10	180.0 µM	1.32E-36	cell wall signaling	2	1.51E-73

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.777	0	YJL095W	BCK1	hom	Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.417	2.91E-140	YPR165W	RHO1	het	GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.360	9.86E-103	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.358	3.18E-101	YLR331C_d	JIP3_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.347	4.45E-95	YLR332W	MID2	hom	O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.307	1.40E-73	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.276	3.92E-59	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.269	4.04E-56	YOR008C	SLG1	hom	Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.266	4.18E-55	YPL089C	RLM1	hom	MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
0.261	6.20E-53	YLL043W	FPS1	hom	Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.242	1.26E-45	YJL099W	CHS6	hom	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.231	1.22E-41	YBL006C	LDB7	hom	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.222	1.48E-38	YPR107C	YTH1	het	Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
0.221	4.08E-38	YDR348C	PAL1	hom	Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.220	9.68E-38	YCR094W	CDC50	hom	Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication