YHR031C / RRM3

DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR031C / RRM3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:3.74|P-value:2.77E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:5.42|P-value:2.76E-7 ID:SGTC_98|Compound:0074-0023|FD-Score:3.46|P-value:7.11E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.52|P-value:1.51E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:5.18|P-value:8.60E-7 ID:SGTC_647|Compound:0140-0236|FD-Score:3.79|P-value:2.33E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.14|P-value:6.50E-5 ID:SGTC_726|Compound:3448-4391|FD-Score:6.87|P-value:1.05E-10 ID:SGTC_829|Compound:1635-0120|FD-Score:4.89|P-value:3.13E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:6.19|P-value:5.19E-9 ID:SGTC_990|Compound:3985-0011|FD-Score:-3.53|P-value:5.25E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:5.03|P-value:1.69E-6 ID:SGTC_992|Compound:3909-8734|FD-Score:-3.93|P-value:1.33E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.41|P-value:8.23E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:4.33|P-value:3.22E-5 ID:SGTC_1666|Compound:st014829|FD-Score:3.36|P-value:9.75E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-3.84|P-value:1.83E-4 ID:SGTC_1736|Compound:st036774|FD-Score:-3.85|P-value:1.76E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.57|P-value:4.90E-4 ID:SGTC_2023|Compound:5144124|FD-Score:5.89|P-value:2.63E-8 ID:SGTC_2088|Compound:5278280|FD-Score:4.45|P-value:1.95E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.96|P-value:1.30E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:5.33|P-value:4.25E-7 ID:SGTC_2396|Compound:5342950|FD-Score:5.02|P-value:1.80E-6 ID:SGTC_2445|Compound:5742597|FD-Score:-3.56|P-value:4.73E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:-3.52|P-value:5.40E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-3.73|P-value:2.64E-4 ID:SGTC_2869|Compound:9037858|FD-Score:3.42|P-value:7.92E-4 ID:SGTC_2879|Compound:9041815|FD-Score:-3.36|P-value:9.15E-4 ID:SGTC_2900|Compound:5328528|FD-Score:3.46|P-value:7.14E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.04|P-value:9.00E-5 ID:SGTC_3247|Compound:9135149|FD-Score:4.18|P-value:5.66E-5 ID:SGTC_3265|Compound:9137266|FD-Score:-4.09|P-value:7.43E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.74|P-value:2.77E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:5.42|P-value:2.76E-7 ID:SGTC_98|Compound:0074-0023|FD-Score:3.46|P-value:7.11E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.52|P-value:1.51E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:5.18|P-value:8.60E-7 ID:SGTC_647|Compound:0140-0236|FD-Score:3.79|P-value:2.33E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.14|P-value:6.50E-5 ID:SGTC_726|Compound:3448-4391|FD-Score:6.87|P-value:1.05E-10 ID:SGTC_829|Compound:1635-0120|FD-Score:4.89|P-value:3.13E-6 ID:SGTC_839|Compound:0122-0028|FD-Score:6.19|P-value:5.19E-9 ID:SGTC_990|Compound:3985-0011|FD-Score:-3.53|P-value:5.25E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:5.03|P-value:1.69E-6 ID:SGTC_992|Compound:3909-8734|FD-Score:-3.93|P-value:1.33E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.41|P-value:8.23E-4 ID:SGTC_1225|Compound:1216-0074|FD-Score:4.33|P-value:3.22E-5 ID:SGTC_1666|Compound:st014829|FD-Score:3.36|P-value:9.75E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-3.84|P-value:1.83E-4 ID:SGTC_1736|Compound:st036774|FD-Score:-3.85|P-value:1.76E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.57|P-value:4.90E-4 ID:SGTC_2023|Compound:5144124|FD-Score:5.89|P-value:2.63E-8 ID:SGTC_2088|Compound:5278280|FD-Score:4.45|P-value:1.95E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.96|P-value:1.30E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:5.33|P-value:4.25E-7 ID:SGTC_2396|Compound:5342950|FD-Score:5.02|P-value:1.80E-6 ID:SGTC_2445|Compound:5742597|FD-Score:-3.56|P-value:4.73E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:-3.52|P-value:5.40E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-3.73|P-value:2.64E-4 ID:SGTC_2869|Compound:9037858|FD-Score:3.42|P-value:7.92E-4 ID:SGTC_2879|Compound:9041815|FD-Score:-3.36|P-value:9.15E-4 ID:SGTC_2900|Compound:5328528|FD-Score:3.46|P-value:7.14E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-4.04|P-value:9.00E-5 ID:SGTC_3247|Compound:9135149|FD-Score:4.18|P-value:5.66E-5 ID:SGTC_3265|Compound:9137266|FD-Score:-4.09|P-value:7.43E-5

Top fitness defect scores for YHR031C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_726 3448-4391 6.87 34.2 µM 1.05E-10 1 3.12E-12
2 SGTC_839 0122-0028 6.19 230.0 µM 5.19E-9 RSC complex & mRNA processing 5 2.92E-10
3 SGTC_2023 5144124 5.89 124.0 µM 2.63E-8 1 1.93E-9
4 SGTC_93 0109-0045 5.42 59.2 µM 2.76E-7 DNA damage response 18 2.98E-8
5 SGTC_2392 N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide 5.33 200.0 µM 4.25E-7 1 4.93E-8
6 SGTC_514 4466-0038 5.18 2.6 nM 8.60E-7 cell wall 101 1.12E-7
7 SGTC_991 3996-0057 5.03 64.7 µM 1.69E-6 6 2.45E-7
8 SGTC_2396 5342950 5.02 42.8 µM 1.80E-6 RSC complex & mRNA processing 22 2.64E-7
9 SGTC_829 1635-0120 4.89 117.0 µM 3.13E-6 DNA damage response 24 5.02E-7
10 SGTC_423 5-fluorouracil 4.52 457.0 µM 1.51E-5 exosome 12 3.13E-6
11 SGTC_2088 5278280 4.45 127.4 µM 1.95E-5 12 4.21E-6
12 SGTC_1225 1216-0074 4.33 20.6 µM 3.22E-5 19 7.53E-6
13 SGTC_3247 9135149 4.18 49.5 µM 5.66E-5 DNA damage response 21 1.45E-5
14 SGTC_674 0498-0004 4.14 451.0 µM 6.50E-5 heme requiring 21 1.70E-5
15 SGTC_2111 5486399 3.96 200.0 µM 1.30E-4 46 3.82E-5
16 SGTC_647 0140-0236 3.79 81.0 µM 2.33E-4 10 7.49E-5
17 SGTC_58 0957-0393 3.74 9.0 µM 2.77E-4 cell wall 396 9.14E-5
18 SGTC_1915 st067105 3.57 30.6 µM 4.90E-4 RNA pol III & RNase P/MRP 61 1.77E-4
19 SGTC_98 0074-0023 3.46 107.7 µM 7.11E-4 Golgi 60 2.73E-4
20 SGTC_2900 5328528 3.46 85.3 µM 7.14E-4 Golgi 46 2.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.233 1.41E-42 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.182 2.57E-26 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.164 1.44E-21 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.163 2.32E-21 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.145 2.89E-17 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.143 7.46E-17 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.139 5.62E-16 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.139 6.71E-16 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.138 1.01E-15 YBL092W RPL32 het Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
0.138 1.26E-15 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.137 1.47E-15 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.135 4.86E-15 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.134 5.75E-15 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.131 2.98E-14 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.129 8.40E-14 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p