HIPHOP chemogenomics database

YHR041C / SRB2

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR041C / SRB2

Click on Significant Values for Screen Details

Top fitness defect scores for YHR041C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1876	myriocin	12.40	605.0 nM	2.51E-28		3	8.52E-36
2	SGTC_2688	kanamycin b	9.38	96.7 µM	7.08E-17	calcium & mitochondrial duress	36	3.24E-21
3	SGTC_3221	9128209	8.39	49.5 µM	7.99E-14		1	2.52E-17
4	SGTC_241	5hr heat shock (37°C) + cantharidin	8.16	155.4 µM	3.60E-13	cell wall signaling	16	1.71E-16
5	SGTC_2276	7947513	7.12	42.1 µM	2.03E-10		2	5.51E-13
6	SGTC_121	0557-0495	6.59	61.3 µM	3.58E-9	calcium & mitochondrial duress	12	2.14E-11
7	SGTC_582	0987-0078	6.28	156.0 µM	1.82E-8	calcium & mitochondrial duress	18	1.70E-10
8	SGTC_240	5hr heat shock (37°C) + Streptovitacin	6.25	300.0 µM	2.12E-8	heat shock/prefoldin	16	2.07E-10
9	SGTC_243	5hr heat shock (37°C) + splitomicin	5.88	1.0 µM	1.31E-7	heat shock/prefoldin	15	2.10E-9
10	SGTC_310	1273-0058	5.55	1.1 µM	5.85E-7	mitochondrial stress	158	1.42E-8
11	SGTC_1712	st032288	5.43	53.4 µM	9.88E-7	calcium & mitochondrial duress	94	2.76E-8
12	SGTC_249	wiskostatin	5.27	11.2 µM	1.98E-6		4	6.70E-8
13	SGTC_701	4424-0219	5.24	185.0 µM	2.25E-6	calcium & mitochondrial duress	6	7.87E-8
14	SGTC_2737	bleomycin	5.15	30.5 nM	3.39E-6	Golgi	46	1.33E-7
15	SGTC_239	5hr heat shock (37°C) + mitomycin C	5.11	819.4 µM	3.96E-6	heat shock/prefoldin	17	1.62E-7
16	SGTC_149	3970-0822	4.97	46.1 µM	6.99E-6	tubulin folding & SWR complex	50	3.35E-7
17	SGTC_2271	7849410	4.85	118.6 µM	1.12E-5		7	6.08E-7
18	SGTC_453	enantio-paf c16	4.84	10.0 µM	1.19E-5		94	6.60E-7
19	SGTC_832	4083-0019	4.78	164.0 µM	1.52E-5	translation	48	8.97E-7
20	SGTC_108	0509-0257	4.77	621.1 µM	1.52E-5		26	9.02E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.250	5.07E-49	YCR063W	BUD31	hom	Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.243	2.96E-46	YGR212W	SLI1	hom	N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.241	1.60E-45	YLR200W	YKE2	hom	Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.231	5.14E-42	YGR104C	SRB5	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
0.229	3.72E-41	YDR260C	SWM1	hom	Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.218	2.36E-37	YLR027C	AAT2	hom	Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.180	5.89E-26	YGR285C	ZUO1	hom	Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.177	3.66E-25	YNL098C	RAS2	hom	GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.173	6.49E-24	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.170	4.74E-23	YIL105C	SLM1	hom	Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.169	8.57E-23	YOR001W	RRP6	hom	Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.168	1.21E-22	YDR466W	PKH3	hom	Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.160	1.27E-20	YJL129C	TRK1	hom	Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.159	1.52E-20	YHR064C	SSZ1	hom	Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.159	1.58E-20	YHR059W	FYV4	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin