YHR041C / SRB2

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR041C / SRB2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.40|P-value:9.87E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:4.21|P-value:1.23E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.77|P-value:1.52E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:6.59|P-value:3.58E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:3.66|P-value:7.35E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.97|P-value:6.99E-6 ID:SGTC_212|Compound:3807-4682|FD-Score:4.56|P-value:3.42E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.99|P-value:2.54E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:5.11|P-value:3.96E-6 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:6.25|P-value:2.12E-8 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:8.16|P-value:3.60E-13 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.91|P-value:3.37E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:5.88|P-value:1.31E-7 ID:SGTC_249|Compound:wiskostatin|FD-Score:5.27|P-value:1.98E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.57|P-value:9.60E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:4.16|P-value:1.46E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.55|P-value:5.85E-7 ID:SGTC_394|Compound:menadione|FD-Score:3.97|P-value:2.76E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.84|P-value:1.19E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.02|P-value:2.33E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:6.28|P-value:1.82E-8 ID:SGTC_620|Compound:0109-0052|FD-Score:4.24|P-value:1.08E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.75|P-value:5.55E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:5.24|P-value:2.25E-6 ID:SGTC_801|Compound:0866-0028|FD-Score:4.31|P-value:8.50E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:3.79|P-value:4.90E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.78|P-value:1.52E-5 ID:SGTC_854|Compound:0422-0053|FD-Score:4.59|P-value:3.03E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:4.38|P-value:6.57E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:-4.06|P-value:1.18E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.71|P-value:1.95E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:-4.64|P-value:1.39E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.63|P-value:4.94E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.13|P-value:1.60E-4 ID:SGTC_1627|Compound:st003713|FD-Score:4.04|P-value:2.17E-4 ID:SGTC_1712|Compound:st032288|FD-Score:5.43|P-value:9.88E-7 ID:SGTC_1876|Compound:myriocin|FD-Score:12.40|P-value:2.51E-28 ID:SGTC_1909|Compound:st060837|FD-Score:3.83|P-value:4.36E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.62|P-value:8.32E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.13|P-value:1.62E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-3.99|P-value:1.52E-4 ID:SGTC_2267|Compound:7931562|FD-Score:4.36|P-value:7.18E-5 ID:SGTC_2269|Compound:7935389|FD-Score:-3.95|P-value:1.72E-4 ID:SGTC_2271|Compound:7849410|FD-Score:4.85|P-value:1.12E-5 ID:SGTC_2276|Compound:7947513|FD-Score:7.12|P-value:2.03E-10 ID:SGTC_2289|Compound:7946404|FD-Score:3.68|P-value:6.91E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.11|P-value:1.73E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-4.31|P-value:4.74E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.51|P-value:4.21E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.38|P-value:7.08E-17 ID:SGTC_2737|Compound:bleomycin|FD-Score:5.15|P-value:3.39E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.54|P-value:3.74E-5 ID:SGTC_2838|Compound:9002687|FD-Score:-3.71|P-value:3.78E-4 ID:SGTC_2916|Compound:7875993|FD-Score:4.45|P-value:5.19E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.73|P-value:5.83E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.04|P-value:2.17E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.77|P-value:3.10E-4 ID:SGTC_3075|Compound:9116269|FD-Score:4.42|P-value:5.82E-5 ID:SGTC_3081|Compound:9117243|FD-Score:-5.05|P-value:2.58E-6 ID:SGTC_3135|Compound:9127742|FD-Score:4.17|P-value:1.41E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-6.20|P-value:1.23E-8 ID:SGTC_3219|Compound:9131112|FD-Score:-7.47|P-value:1.02E-11 ID:SGTC_3221|Compound:9128209|FD-Score:8.39|P-value:7.99E-14 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.40|P-value:9.87E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:4.21|P-value:1.23E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.77|P-value:1.52E-5 ID:SGTC_121|Compound:0557-0495|FD-Score:6.59|P-value:3.58E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:3.66|P-value:7.35E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.97|P-value:6.99E-6 ID:SGTC_212|Compound:3807-4682|FD-Score:4.56|P-value:3.42E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.99|P-value:2.54E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:5.11|P-value:3.96E-6 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:6.25|P-value:2.12E-8 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:8.16|P-value:3.60E-13 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.91|P-value:3.37E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:5.88|P-value:1.31E-7 ID:SGTC_249|Compound:wiskostatin|FD-Score:5.27|P-value:1.98E-6 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.57|P-value:9.60E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:4.16|P-value:1.46E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.55|P-value:5.85E-7 ID:SGTC_394|Compound:menadione|FD-Score:3.97|P-value:2.76E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.84|P-value:1.19E-5 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.02|P-value:2.33E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:6.28|P-value:1.82E-8 ID:SGTC_620|Compound:0109-0052|FD-Score:4.24|P-value:1.08E-4 ID:SGTC_691|Compound:1548-0686|FD-Score:3.75|P-value:5.55E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:5.24|P-value:2.25E-6 ID:SGTC_801|Compound:0866-0028|FD-Score:4.31|P-value:8.50E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:3.79|P-value:4.90E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.78|P-value:1.52E-5 ID:SGTC_854|Compound:0422-0053|FD-Score:4.59|P-value:3.03E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:4.38|P-value:6.57E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:-4.06|P-value:1.18E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.71|P-value:1.95E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:-4.64|P-value:1.39E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:-3.63|P-value:4.94E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.13|P-value:1.60E-4 ID:SGTC_1627|Compound:st003713|FD-Score:4.04|P-value:2.17E-4 ID:SGTC_1712|Compound:st032288|FD-Score:5.43|P-value:9.88E-7 ID:SGTC_1876|Compound:myriocin|FD-Score:12.40|P-value:2.51E-28 ID:SGTC_1909|Compound:st060837|FD-Score:3.83|P-value:4.36E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.62|P-value:8.32E-4 ID:SGTC_2111|Compound:5486399|FD-Score:4.13|P-value:1.62E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-3.99|P-value:1.52E-4 ID:SGTC_2267|Compound:7931562|FD-Score:4.36|P-value:7.18E-5 ID:SGTC_2269|Compound:7935389|FD-Score:-3.95|P-value:1.72E-4 ID:SGTC_2271|Compound:7849410|FD-Score:4.85|P-value:1.12E-5 ID:SGTC_2276|Compound:7947513|FD-Score:7.12|P-value:2.03E-10 ID:SGTC_2289|Compound:7946404|FD-Score:3.68|P-value:6.91E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.11|P-value:1.73E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-4.31|P-value:4.74E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.51|P-value:4.21E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.38|P-value:7.08E-17 ID:SGTC_2737|Compound:bleomycin|FD-Score:5.15|P-value:3.39E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.54|P-value:3.74E-5 ID:SGTC_2838|Compound:9002687|FD-Score:-3.71|P-value:3.78E-4 ID:SGTC_2916|Compound:7875993|FD-Score:4.45|P-value:5.19E-5 ID:SGTC_2929|Compound:9013348|FD-Score:3.73|P-value:5.83E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.04|P-value:2.17E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.77|P-value:3.10E-4 ID:SGTC_3075|Compound:9116269|FD-Score:4.42|P-value:5.82E-5 ID:SGTC_3081|Compound:9117243|FD-Score:-5.05|P-value:2.58E-6 ID:SGTC_3135|Compound:9127742|FD-Score:4.17|P-value:1.41E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-6.20|P-value:1.23E-8 ID:SGTC_3219|Compound:9131112|FD-Score:-7.47|P-value:1.02E-11 ID:SGTC_3221|Compound:9128209|FD-Score:8.39|P-value:7.99E-14

Top fitness defect scores for YHR041C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1876 myriocin 12.40 605.0 nM 2.51E-28 3 8.52E-36
2 SGTC_2688 kanamycin b 9.38 96.7 µM 7.08E-17 calcium & mitochondrial duress 36 3.24E-21
3 SGTC_3221 9128209 8.39 49.5 µM 7.99E-14 1 2.52E-17
4 SGTC_241 5hr heat shock (37°C) + cantharidin 8.16 155.4 µM 3.60E-13 cell wall signaling 16 1.71E-16
5 SGTC_2276 7947513 7.12 42.1 µM 2.03E-10 2 5.51E-13
6 SGTC_121 0557-0495 6.59 61.3 µM 3.58E-9 calcium & mitochondrial duress 12 2.14E-11
7 SGTC_582 0987-0078 6.28 156.0 µM 1.82E-8 calcium & mitochondrial duress 18 1.70E-10
8 SGTC_240 5hr heat shock (37°C) + Streptovitacin 6.25 300.0 µM 2.12E-8 heat shock/prefoldin 16 2.07E-10
9 SGTC_243 5hr heat shock (37°C) + splitomicin 5.88 1.0 µM 1.31E-7 heat shock/prefoldin 15 2.10E-9
10 SGTC_310 1273-0058 5.55 1.1 µM 5.85E-7 mitochondrial stress 158 1.42E-8
11 SGTC_1712 st032288 5.43 53.4 µM 9.88E-7 calcium & mitochondrial duress 94 2.76E-8
12 SGTC_249 wiskostatin 5.27 11.2 µM 1.98E-6 4 6.70E-8
13 SGTC_701 4424-0219 5.24 185.0 µM 2.25E-6 calcium & mitochondrial duress 6 7.87E-8
14 SGTC_2737 bleomycin 5.15 30.5 nM 3.39E-6 Golgi 46 1.33E-7
15 SGTC_239 5hr heat shock (37°C) + mitomycin C 5.11 819.4 µM 3.96E-6 heat shock/prefoldin 17 1.62E-7
16 SGTC_149 3970-0822 4.97 46.1 µM 6.99E-6 tubulin folding & SWR complex 50 3.35E-7
17 SGTC_2271 7849410 4.85 118.6 µM 1.12E-5 7 6.08E-7
18 SGTC_453 enantio-paf c16 4.84 10.0 µM 1.19E-5 94 6.60E-7
19 SGTC_832 4083-0019 4.78 164.0 µM 1.52E-5 translation 48 8.97E-7
20 SGTC_108 0509-0257 4.77 621.1 µM 1.52E-5 26 9.02E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.250 5.07E-49 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.243 2.96E-46 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.241 1.60E-45 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.231 5.14E-42 YGR104C SRB5 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
0.229 3.72E-41 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.218 2.36E-37 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.180 5.89E-26 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.177 3.66E-25 YNL098C RAS2 hom GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.173 6.49E-24 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.170 4.74E-23 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.169 8.57E-23 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.168 1.21E-22 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.160 1.27E-20 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.159 1.52E-20 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
0.159 1.58E-20 YHR059W FYV4 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin