YHR075C / PPE1

Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR075C / PPE1

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:-3.06|P-value:8.93E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.44|P-value:2.32E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.34|P-value:3.44E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.05|P-value:9.61E-4 ID:SGTC_83|Compound:0170-0301|FD-Score:-3.51|P-value:1.73E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.07|P-value:1.72E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:4.38|P-value:4.10E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.14|P-value:7.10E-4 ID:SGTC_606|Compound:k015-0008|FD-Score:3.67|P-value:9.25E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:4.05|P-value:1.85E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:3.80|P-value:5.60E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:3.14|P-value:6.94E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.12|P-value:7.61E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:-3.19|P-value:5.72E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.06|P-value:9.33E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.15|P-value:6.66E-4 ID:SGTC_1604|Compound:st002052|FD-Score:4.64|P-value:1.17E-6 ID:SGTC_1607|Compound:st000305|FD-Score:-3.92|P-value:3.31E-5 ID:SGTC_1610|Compound:st001799|FD-Score:3.45|P-value:2.26E-4 ID:SGTC_1615|Compound:st002656|FD-Score:4.33|P-value:5.29E-6 ID:SGTC_1623|Compound:st003709|FD-Score:-3.73|P-value:7.10E-5 ID:SGTC_1855|Compound:st056195|FD-Score:-3.39|P-value:2.75E-4 ID:SGTC_1861|Compound:st057647|FD-Score:3.73|P-value:7.27E-5 ID:SGTC_1868|Compound:st057644|FD-Score:-3.19|P-value:5.82E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.28|P-value:4.20E-4 ID:SGTC_2064|Compound:5220758|FD-Score:4.59|P-value:1.51E-6 ID:SGTC_2102|Compound:5377816|FD-Score:3.17|P-value:6.41E-4 ID:SGTC_2176|Compound:5809774|FD-Score:3.94|P-value:3.04E-5 ID:SGTC_2411|Compound:st077133|FD-Score:3.12|P-value:7.65E-4 ID:SGTC_2451|Compound:5856223|FD-Score:3.16|P-value:6.49E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.58|P-value:1.34E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.77|P-value:6.07E-5 ID:SGTC_2803|Compound:7707263|FD-Score:3.42|P-value:2.50E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.38|P-value:2.91E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.56|P-value:1.46E-4 ID:SGTC_2995|Compound:9067767|FD-Score:3.21|P-value:5.47E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.90|P-value:3.67E-5 ID:SGTC_3050|Compound:9093513|FD-Score:4.66|P-value:1.06E-6 ID:SGTC_3063|Compound:1327-0034|FD-Score:-3.07|P-value:8.71E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.44|P-value:2.30E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.25|P-value:4.76E-4 ID:SGTC_3206|Compound:9114350|FD-Score:3.59|P-value:1.29E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:-3.06|P-value:8.93E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-3.44|P-value:2.32E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:3.34|P-value:3.44E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.05|P-value:9.61E-4 ID:SGTC_83|Compound:0170-0301|FD-Score:-3.51|P-value:1.73E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.07|P-value:1.72E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:4.38|P-value:4.10E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.14|P-value:7.10E-4 ID:SGTC_606|Compound:k015-0008|FD-Score:3.67|P-value:9.25E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:4.05|P-value:1.85E-5 ID:SGTC_937|Compound:3122-0005|FD-Score:3.80|P-value:5.60E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:3.14|P-value:6.94E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.12|P-value:7.61E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:-3.19|P-value:5.72E-4 ID:SGTC_1441|Compound:4106-0040|FD-Score:3.06|P-value:9.33E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.15|P-value:6.66E-4 ID:SGTC_1604|Compound:st002052|FD-Score:4.64|P-value:1.17E-6 ID:SGTC_1607|Compound:st000305|FD-Score:-3.92|P-value:3.31E-5 ID:SGTC_1610|Compound:st001799|FD-Score:3.45|P-value:2.26E-4 ID:SGTC_1615|Compound:st002656|FD-Score:4.33|P-value:5.29E-6 ID:SGTC_1623|Compound:st003709|FD-Score:-3.73|P-value:7.10E-5 ID:SGTC_1855|Compound:st056195|FD-Score:-3.39|P-value:2.75E-4 ID:SGTC_1861|Compound:st057647|FD-Score:3.73|P-value:7.27E-5 ID:SGTC_1868|Compound:st057644|FD-Score:-3.19|P-value:5.82E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.28|P-value:4.20E-4 ID:SGTC_2064|Compound:5220758|FD-Score:4.59|P-value:1.51E-6 ID:SGTC_2102|Compound:5377816|FD-Score:3.17|P-value:6.41E-4 ID:SGTC_2176|Compound:5809774|FD-Score:3.94|P-value:3.04E-5 ID:SGTC_2411|Compound:st077133|FD-Score:3.12|P-value:7.65E-4 ID:SGTC_2451|Compound:5856223|FD-Score:3.16|P-value:6.49E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.58|P-value:1.34E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:-3.77|P-value:6.07E-5 ID:SGTC_2803|Compound:7707263|FD-Score:3.42|P-value:2.50E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.38|P-value:2.91E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.56|P-value:1.46E-4 ID:SGTC_2995|Compound:9067767|FD-Score:3.21|P-value:5.47E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.90|P-value:3.67E-5 ID:SGTC_3050|Compound:9093513|FD-Score:4.66|P-value:1.06E-6 ID:SGTC_3063|Compound:1327-0034|FD-Score:-3.07|P-value:8.71E-4 ID:SGTC_3078|Compound:9116413|FD-Score:3.44|P-value:2.30E-4 ID:SGTC_3166|Compound:9102297|FD-Score:3.25|P-value:4.76E-4 ID:SGTC_3206|Compound:9114350|FD-Score:3.59|P-value:1.29E-4

Top fitness defect scores for YHR075C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3050 9093513 4.66 49.5 µM 1.06E-6 9 1.59E-6
2 SGTC_1604 st002052 4.64 72.4 µM 1.17E-6 TSC3-RPN4 16 1.75E-6
3 SGTC_2064 5220758 4.59 200.0 µM 1.51E-6 18 2.23E-6
4 SGTC_409 arachidonic acid 4.38 100.0 µM 4.10E-6 15 5.85E-6
5 SGTC_1615 st002656 4.33 86.5 µM 5.29E-6 redox potentiating 7 7.49E-6
6 SGTC_138 0850-0259 4.07 40.9 µM 1.72E-5 cell wall 192 2.34E-5
7 SGTC_683 panaxydol 4.05 45.0 µM 1.85E-5 sphingolipid biosynthesis & PDR1 37 2.52E-5
8 SGTC_2176 5809774 3.94 34.0 µM 3.04E-5 mitochondrial processes 11 4.05E-5
9 SGTC_2999 9069765 3.90 71.4 µM 3.67E-5 amide catabolism 26 4.87E-5
10 SGTC_937 3122-0005 3.80 46.7 µM 5.60E-5 28 7.32E-5
11 SGTC_1861 st057647 3.73 17.4 µM 7.27E-5 27 9.43E-5
12 SGTC_606 k015-0008 3.67 38.6 µM 9.25E-5 22 1.19E-4
13 SGTC_3206 9114350 3.59 49.5 µM 1.29E-4 27 1.64E-4
14 SGTC_2556 citropten 3.58 84.6 µM 1.34E-4 36 1.70E-4
15 SGTC_2946 9065502 3.56 71.4 µM 1.46E-4 13 1.85E-4
16 SGTC_1610 st001799 3.45 48.1 µM 2.26E-4 24 2.83E-4
17 SGTC_3078 9116413 3.44 49.5 µM 2.30E-4 unfolded protein response 54 2.88E-4
18 SGTC_2803 7707263 3.42 71.4 µM 2.50E-4 47 3.12E-4
19 SGTC_2943 9068529 3.38 45.3 µM 2.91E-4 42 3.61E-4
20 SGTC_44 0929-0055 3.34 61.2 µM 3.44E-4 54 4.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 5.80E-11 YDL188C PPH22 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
0.107 5.38E-10 YBR030W RKM3 hom Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein
0.103 2.04E-9 YJR111C_p YJR111C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
0.098 1.14E-8 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.094 4.41E-8 YHR129C ARP1 hom Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
0.094 4.72E-8 YLL021W SPA2 hom Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate
0.093 6.95E-8 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.093 7.59E-8 YER114C BOI2 hom Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
0.090 2.03E-7 YLR138W NHA1 hom Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
0.089 2.42E-7 YDR540C IRC4 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.089 2.55E-7 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.087 4.29E-7 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.087 4.31E-7 YMR152W YIM1 hom Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
0.085 7.05E-7 YDR016C DAD1 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
0.085 8.99E-7 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth