HIPHOP chemogenomics database

YHR077C / NMD2

Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR077C / NMD2

Click on Significant Values for Screen Details

Top fitness defect scores for YHR077C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2606	farnesol	6.97	9.8 µM	1.04E-12		2	1.60E-12
2	SGTC_1528	arabinosylhypoxanthine	5.95	74.6 µM	9.84E-10		4	1.34E-9
3	SGTC_1081	sertraline	5.82	6.0 µM	2.16E-9	60S ribosome export	11	2.91E-9
4	SGTC_1361	1608-0041	5.14	5.6 µM	1.07E-7	RPP1 & pyrimidine depletion	6	1.35E-7
5	SGTC_133	0724-0033	4.96	378.9 µM	2.88E-7		10	3.57E-7
6	SGTC_568	1193-0097	4.65	7.5 µM	1.41E-6	ERAD & cell cycle	104	1.70E-6
7	SGTC_1855	st056195	4.54	39.6 µM	2.34E-6	60S ribosome export	8	2.80E-6
8	SGTC_1750	st081859	4.49	2.4 µM	3.04E-6	endomembrane recycling	153	3.61E-6
9	SGTC_183	3914-0051	4.43	30.6 µM	3.90E-6	60S ribosome export	12	4.62E-6
10	SGTC_2608	thymoquinone	4.21	42.7 µM	1.08E-5		13	1.26E-5
11	SGTC_3178	9107506	4.16	49.5 µM	1.37E-5		10	1.59E-5
12	SGTC_3090	9117696	4.14	49.5 µM	1.52E-5		14	1.77E-5
13	SGTC_1050	3232-1156	4.11	69.3 µM	1.69E-5	RSC complex & mRNA processing	17	1.96E-5
14	SGTC_66	0109-0187	4.10	42.4 µM	1.76E-5		35	2.03E-5
15	SGTC_591	4299-0174	4.00	154.0 µM	2.78E-5		8	3.19E-5
16	SGTC_1523	st012844	3.80	44.7 µM	6.30E-5	60S ribosome export	25	7.13E-5
17	SGTC_1970	st070122	3.74	35.8 µM	8.00E-5	TSC3-RPN4	31	9.02E-5
18	SGTC_2014	4013930	3.73	200.0 µM	8.46E-5		41	9.53E-5
19	SGTC_263	alizarin	3.68	107.9 µM	1.05E-4	iron homeostasis	70	1.18E-4
20	SGTC_453	enantio-paf c16	3.57	10.0 µM	1.58E-4		180	1.76E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.167	2.48E-22	YMR126C_p	DLT1_p	hom	Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.163	1.87E-21	YER171W	RAD3	het	5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.145	3.11E-17	YPR153W	YPR153W	hom	Putative protein of unknown function
0.131	2.79E-14	YKL098W	MTC2	hom	Protein of unknown function; mtc2 is synthetically sick with cdc13-1
0.123	7.58E-13	YPL077C_p	YPL077C_p	hom	Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
0.109	2.88E-10	YER066W_p	RRT13_p	hom	Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.108	3.16E-10	YGL146C_p	RRT6_p	hom	Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.102	2.85E-9	YOL011W	PLB3	hom	Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro
0.102	3.79E-9	YMR135C	GID8	hom	Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
0.099	8.49E-9	YHR151C	MTC6	hom	Protein of unknown function; mtc6 is synthetically sick with cdc13-1
0.099	8.96E-9	YBR255W	MTC4	hom	Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
0.096	2.36E-8	YMR182C	RGM1	hom	Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y-prime telomeric elements and subtelomeric COS genes; RGM1 has a paralog, USV1, that arose from the whole genome duplication
0.096	2.89E-8	YLR395C	COX8	hom	Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.092	9.37E-8	YOR300W_d	YOR300W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.090	1.53E-7	YBR058C	UBP14	hom	Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T