YHR086W / NAM8

RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR086W / NAM8

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:-3.53|P-value:1.10E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:-3.41|P-value:1.76E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.96|P-value:9.63E-4 ID:SGTC_647|Compound:0140-0236|FD-Score:-3.40|P-value:1.86E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.07|P-value:8.13E-4 ID:SGTC_1115|Compound:galangin|FD-Score:3.73|P-value:6.22E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:-3.57|P-value:9.46E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.49|P-value:1.69E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.03|P-value:9.51E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.02|P-value:9.87E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.05|P-value:8.71E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:3.68|P-value:7.66E-5 ID:SGTC_1567|Compound:st069303|FD-Score:3.33|P-value:3.14E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.41|P-value:2.28E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-3.77|P-value:4.11E-5 ID:SGTC_1781|Compound:st038725|FD-Score:-3.18|P-value:4.39E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.20|P-value:5.15E-4 ID:SGTC_1960|Compound:st011366|FD-Score:4.34|P-value:4.00E-6 ID:SGTC_1968|Compound:st069590|FD-Score:3.17|P-value:5.61E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.15|P-value:4.86E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.19|P-value:5.26E-4 ID:SGTC_2184|Compound:6075246|FD-Score:3.05|P-value:8.83E-4 ID:SGTC_2317|Compound:5986382|FD-Score:-3.44|P-value:1.61E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.51|P-value:1.72E-6 ID:SGTC_2358|Compound:9037157|FD-Score:3.45|P-value:1.94E-4 ID:SGTC_2396|Compound:5342950|FD-Score:-3.06|P-value:6.79E-4 ID:SGTC_2398|Compound:5228213|FD-Score:7.38|P-value:1.23E-14 ID:SGTC_2399|Compound:5884133|FD-Score:3.26|P-value:4.07E-4 ID:SGTC_2479|Compound:5617353|FD-Score:5.18|P-value:4.57E-8 ID:SGTC_2485|Compound:5633444|FD-Score:-3.46|P-value:1.46E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-4.19|P-value:5.94E-6 ID:SGTC_2576|Compound:guaiol|FD-Score:3.66|P-value:8.46E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.46|P-value:1.48E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.35|P-value:2.90E-4 ID:SGTC_2926|Compound:9010726|FD-Score:-4.53|P-value:1.14E-6 ID:SGTC_3004|Compound:9076122|FD-Score:4.34|P-value:3.94E-6 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.53|P-value:1.10E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:-3.41|P-value:1.76E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.96|P-value:9.63E-4 ID:SGTC_647|Compound:0140-0236|FD-Score:-3.40|P-value:1.86E-4 ID:SGTC_890|Compound:0929-0006|FD-Score:3.07|P-value:8.13E-4 ID:SGTC_1115|Compound:galangin|FD-Score:3.73|P-value:6.22E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:-3.57|P-value:9.46E-5 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.49|P-value:1.69E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.03|P-value:9.51E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.02|P-value:9.87E-4 ID:SGTC_1405|Compound:3909-7894|FD-Score:3.05|P-value:8.71E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:3.68|P-value:7.66E-5 ID:SGTC_1567|Compound:st069303|FD-Score:3.33|P-value:3.14E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:3.41|P-value:2.28E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-3.77|P-value:4.11E-5 ID:SGTC_1781|Compound:st038725|FD-Score:-3.18|P-value:4.39E-4 ID:SGTC_1941|Compound:st076221|FD-Score:3.20|P-value:5.15E-4 ID:SGTC_1960|Compound:st011366|FD-Score:4.34|P-value:4.00E-6 ID:SGTC_1968|Compound:st069590|FD-Score:3.17|P-value:5.61E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.15|P-value:4.86E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.19|P-value:5.26E-4 ID:SGTC_2184|Compound:6075246|FD-Score:3.05|P-value:8.83E-4 ID:SGTC_2317|Compound:5986382|FD-Score:-3.44|P-value:1.61E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.51|P-value:1.72E-6 ID:SGTC_2358|Compound:9037157|FD-Score:3.45|P-value:1.94E-4 ID:SGTC_2396|Compound:5342950|FD-Score:-3.06|P-value:6.79E-4 ID:SGTC_2398|Compound:5228213|FD-Score:7.38|P-value:1.23E-14 ID:SGTC_2399|Compound:5884133|FD-Score:3.26|P-value:4.07E-4 ID:SGTC_2479|Compound:5617353|FD-Score:5.18|P-value:4.57E-8 ID:SGTC_2485|Compound:5633444|FD-Score:-3.46|P-value:1.46E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:-4.19|P-value:5.94E-6 ID:SGTC_2576|Compound:guaiol|FD-Score:3.66|P-value:8.46E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-3.46|P-value:1.48E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.35|P-value:2.90E-4 ID:SGTC_2926|Compound:9010726|FD-Score:-4.53|P-value:1.14E-6 ID:SGTC_3004|Compound:9076122|FD-Score:4.34|P-value:3.94E-6

Top fitness defect scores for YHR086W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2398 5228213 7.38 48.8 µM 1.23E-14 iron homeostasis 3 7.71E-14
2 SGTC_2479 5617353 5.18 43.9 µM 4.57E-8 13 1.08E-7
3 SGTC_2329 6138490 4.51 200.0 µM 1.72E-6 15 3.26E-6
4 SGTC_3004 9076122 4.34 71.4 µM 3.94E-6 12 7.10E-6
5 SGTC_1960 st011366 4.34 51.8 µM 4.00E-6 5 7.19E-6
6 SGTC_1115 galangin 3.73 695.7 nM 6.22E-5 RPP1 & pyrimidine depletion 22 9.49E-5
7 SGTC_1408 3909-7976 3.68 48.8 µM 7.66E-5 Golgi 31 1.15E-4
8 SGTC_2576 guaiol 3.66 23.1 µM 8.46E-5 32 1.27E-4
9 SGTC_1293 1000-1278 3.49 195.0 µM 1.69E-4 26 2.43E-4
10 SGTC_2358 9037157 3.45 4.0 µM 1.94E-4 50 2.76E-4
11 SGTC_1598 3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin 3.41 19.1 µM 2.28E-4 44 3.22E-4
12 SGTC_2829 7992743 3.35 58.4 µM 2.90E-4 60 4.04E-4
13 SGTC_1567 st069303 3.33 68.4 µM 3.14E-4 mitochondrial processes 42 4.36E-4
14 SGTC_2399 5884133 3.26 200.0 µM 4.07E-4 cell wall signaling 110 5.57E-4
15 SGTC_1941 st076221 3.20 61.8 µM 5.15E-4 sphingolipid biosynthesis & PDR1 121 6.95E-4
16 SGTC_2156 5649887 3.19 115.0 µM 5.26E-4 RPP1 & pyrimidine depletion 39 7.08E-4
17 SGTC_1968 st069590 3.17 43.6 µM 5.61E-4 34 7.53E-4
18 SGTC_890 0929-0006 3.07 47.2 µM 8.13E-4 44 0.00107
19 SGTC_1405 3909-7894 3.05 12.0 µM 8.71E-4 60S ribosome export 38 0.00114
20 SGTC_2184 6075246 3.05 200.0 µM 8.83E-4 59 0.00115

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 3.26E-20 YHR101C BIG1 het Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
0.150 2.22E-18 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.142 1.19E-16 YMR092C AIP1 hom Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
0.128 9.22E-14 YOR342C YOR342C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication
0.123 7.44E-13 YBR254C TRS20 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
0.113 4.81E-11 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.112 6.57E-11 YJR112W NNF1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
0.110 1.54E-10 YPL074W YTA6 hom Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
0.109 2.51E-10 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.105 1.00E-9 YLR326W_p YLR326W_p hom Putative protein of unknown function, predicted to be palmitoylated
0.100 6.91E-9 YPL177C CUP9 hom Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.098 1.28E-8 YER062C HOR2 hom Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication
0.097 1.75E-8 YDR154C_d YDR154C_d hom Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein
0.096 2.84E-8 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
0.096 2.75E-8 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress