YHR111W / UBA4

Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR111W / UBA4

Click on Significant Values for Screen Details ID:SGTC_91|Compound:0417-1751|FD-Score:4.71|P-value:7.93E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:4.31|P-value:3.76E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.56|P-value:1.45E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:5.52|P-value:2.16E-7 ID:SGTC_378|Compound:3970-0959|FD-Score:3.64|P-value:4.03E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.42|P-value:2.46E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.37|P-value:9.57E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.70|P-value:4.79E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:4.53|P-value:1.63E-5 ID:SGTC_756|Compound:0508-1999|FD-Score:4.38|P-value:2.94E-5 ID:SGTC_770|Compound:0389-1121|FD-Score:5.23|P-value:8.25E-7 ID:SGTC_879|Compound:0831-1030|FD-Score:4.27|P-value:4.40E-5 ID:SGTC_1096|Compound:rimcazole|FD-Score:4.17|P-value:6.46E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.19|P-value:5.96E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:6.03|P-value:1.72E-8 ID:SGTC_1547|Compound:harmine|FD-Score:-4.24|P-value:7.58E-5 ID:SGTC_1633|Compound:st005664|FD-Score:4.95|P-value:2.78E-6 ID:SGTC_1817|Compound:st052116|FD-Score:3.51|P-value:6.11E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:11.60|P-value:3.36E-26 ID:SGTC_1868|Compound:st057644|FD-Score:3.37|P-value:9.51E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-4.70|P-value:1.32E-5 ID:SGTC_1968|Compound:st069590|FD-Score:-3.47|P-value:9.85E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.91|P-value:2.41E-4 ID:SGTC_2162|Compound:5663267|FD-Score:4.81|P-value:5.13E-6 ID:SGTC_2285|Compound:7945142|FD-Score:4.35|P-value:3.25E-5 ID:SGTC_2297|Compound:7971671|FD-Score:-4.21|P-value:8.36E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.07|P-value:9.15E-5 ID:SGTC_2457|Compound:5731572|FD-Score:5.08|P-value:1.62E-6 ID:SGTC_2473|Compound:5854593|FD-Score:3.89|P-value:1.74E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.98|P-value:1.28E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.49|P-value:9.28E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:8.26|P-value:2.41E-14 ID:SGTC_2686|Compound:alizarin|FD-Score:3.45|P-value:7.32E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.42|P-value:6.24E-12 ID:SGTC_2896|Compound:9052656|FD-Score:-3.96|P-value:2.06E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.57|P-value:5.09E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.54|P-value:5.62E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.60|P-value:4.62E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.39|P-value:8.88E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-4.07|P-value:1.38E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.81|P-value:3.33E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-3.56|P-value:7.65E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.62|P-value:4.33E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:4.71|P-value:7.93E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:4.31|P-value:3.76E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.56|P-value:1.45E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:5.52|P-value:2.16E-7 ID:SGTC_378|Compound:3970-0959|FD-Score:3.64|P-value:4.03E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.42|P-value:2.46E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.37|P-value:9.57E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.70|P-value:4.79E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:4.53|P-value:1.63E-5 ID:SGTC_756|Compound:0508-1999|FD-Score:4.38|P-value:2.94E-5 ID:SGTC_770|Compound:0389-1121|FD-Score:5.23|P-value:8.25E-7 ID:SGTC_879|Compound:0831-1030|FD-Score:4.27|P-value:4.40E-5 ID:SGTC_1096|Compound:rimcazole|FD-Score:4.17|P-value:6.46E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.19|P-value:5.96E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:6.03|P-value:1.72E-8 ID:SGTC_1547|Compound:harmine|FD-Score:-4.24|P-value:7.58E-5 ID:SGTC_1633|Compound:st005664|FD-Score:4.95|P-value:2.78E-6 ID:SGTC_1817|Compound:st052116|FD-Score:3.51|P-value:6.11E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:11.60|P-value:3.36E-26 ID:SGTC_1868|Compound:st057644|FD-Score:3.37|P-value:9.51E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-4.70|P-value:1.32E-5 ID:SGTC_1968|Compound:st069590|FD-Score:-3.47|P-value:9.85E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-3.91|P-value:2.41E-4 ID:SGTC_2162|Compound:5663267|FD-Score:4.81|P-value:5.13E-6 ID:SGTC_2285|Compound:7945142|FD-Score:4.35|P-value:3.25E-5 ID:SGTC_2297|Compound:7971671|FD-Score:-4.21|P-value:8.36E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.07|P-value:9.15E-5 ID:SGTC_2457|Compound:5731572|FD-Score:5.08|P-value:1.62E-6 ID:SGTC_2473|Compound:5854593|FD-Score:3.89|P-value:1.74E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.98|P-value:1.28E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.49|P-value:9.28E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:8.26|P-value:2.41E-14 ID:SGTC_2686|Compound:alizarin|FD-Score:3.45|P-value:7.32E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.42|P-value:6.24E-12 ID:SGTC_2896|Compound:9052656|FD-Score:-3.96|P-value:2.06E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.57|P-value:5.09E-4 ID:SGTC_2964|Compound:9083345|FD-Score:3.54|P-value:5.62E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.60|P-value:4.62E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.39|P-value:8.88E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-4.07|P-value:1.38E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.81|P-value:3.33E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-3.56|P-value:7.65E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.62|P-value:4.33E-4

Top fitness defect scores for YHR111W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 11.60 4.0 nM 3.36E-26 27 3.47E-31
2 SGTC_2679 rapamycin 8.26 1.0 nM 2.41E-14 38 7.33E-17
3 SGTC_2688 kanamycin b 7.42 96.7 µM 6.24E-12 calcium & mitochondrial duress 90 5.95E-14
4 SGTC_1284 0929-0043 6.03 16.0 µM 1.72E-8 9 8.19E-10
5 SGTC_314 1671-0008 5.52 2.7 µM 2.16E-7 12 1.70E-8
6 SGTC_770 0389-1121 5.23 67.2 µM 8.25E-7 RPP1 & pyrimidine depletion 7 8.48E-8
7 SGTC_2457 5731572 5.08 11.7 µM 1.62E-6 6 1.90E-7
8 SGTC_1633 st005664 4.95 67.9 µM 2.78E-6 14 3.62E-7
9 SGTC_2162 5663267 4.81 200.0 µM 5.13E-6 5 7.54E-7
10 SGTC_91 0417-1751 4.71 58.4 µM 7.93E-6 Golgi 31 1.27E-6
11 SGTC_269 exalamide 4.56 29.0 µM 1.45E-5 ergosterol biosynthesis 13 2.61E-6
12 SGTC_706 4262-0057 4.53 46.9 µM 1.63E-5 fatty acid desaturase (OLE1) 18 2.99E-6
13 SGTC_467 ro 31-8220 4.42 72.8 µM 2.46E-5 calcium & mitochondrial duress 59 4.90E-6
14 SGTC_756 0508-1999 4.38 28.3 µM 2.94E-5 RPP1 & pyrimidine depletion 14 6.07E-6
15 SGTC_2285 7945142 4.35 200.0 µM 3.25E-5 12 6.84E-6
16 SGTC_118 0083-0097 4.31 135.3 µM 3.76E-5 translation 101 8.13E-6
17 SGTC_879 0831-1030 4.27 162.0 µM 4.40E-5 37 9.79E-6
18 SGTC_1157 4092-0394 4.19 27.6 µM 5.96E-5 25 1.41E-5
19 SGTC_1096 rimcazole 4.17 32.2 µM 6.46E-5 5 1.55E-5
20 SGTC_2420 aureobasidin a 4.07 50.0 nM 9.15E-5 36 2.35E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.292 5.31E-67 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.282 3.41E-62 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.279 6.47E-61 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.278 2.52E-60 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.273 2.12E-58 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.272 7.89E-58 YGR200C ELP2 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.269 1.69E-56 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.266 1.82E-55 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.265 8.90E-55 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.251 3.86E-49 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.241 1.98E-45 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.217 4.16E-37 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.206 2.51E-33 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.205 4.42E-33 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.201 8.32E-32 YMR312W ELP6 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity