HIPHOP chemogenomics database

YHR183W / GND1

6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR183W / GND1

Click on Significant Values for Screen Details

Top fitness defect scores for YHR183W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_69	0096-0274	4.63	206.7 µM	3.35E-6	mitochondrial processes	26	1.83E-6
2	SGTC_577	r071-0003	4.16	38.6 µM	2.58E-5	fatty acid desaturase (OLE1)	46	1.61E-5
3	SGTC_3158	9100007	4.07	49.5 µM	3.67E-5		17	2.34E-5
4	SGTC_2393	9031399	4.01	185.9 µM	4.66E-5	RNA processing & uracil transport	24	3.02E-5
5	SGTC_2223	6903751	3.98	107.6 µM	5.22E-5	superoxide	10	3.41E-5
6	SGTC_1354	1498-1681	3.88	81.0 µM	7.68E-5		22	5.13E-5
7	SGTC_1969	st069479	3.85	40.1 µM	8.65E-5		13	5.83E-5
8	SGTC_272	doxorubicin	3.84	8.1 µM	9.15E-5	anthracycline transcription coupled DNA repair	24	6.19E-5
9	SGTC_2303	7774051	3.83	200.0 µM	9.61E-5		22	6.52E-5
10	SGTC_2358	9037157	3.82	4.0 µM	9.69E-5		36	6.57E-5
11	SGTC_20	0481-0013	3.71	4.9 µM	1.51E-4		31	1.06E-4
12	SGTC_1661	st013063	3.69	9.7 µM	1.57E-4		31	1.10E-4
13	SGTC_100	0086-0128	3.67	49.6 µM	1.72E-4	copper-dependent oxidative stress	62	1.21E-4
14	SGTC_2139	5578692	3.63	56.5 µM	1.97E-4	superoxide	21	1.40E-4
15	SGTC_2845	9003024	3.62	43.0 µM	2.07E-4	amide catabolism	56	1.47E-4
16	SGTC_2408	sulbentine	3.54	18.4 µM	2.76E-4		63	2.00E-4
17	SGTC_581	0958-0271	3.44	211.0 µM	3.96E-4		73	2.93E-4
18	SGTC_2952	9073731	3.42	13.8 µM	4.17E-4	tubulin folding & SWR complex	67	3.10E-4
19	SGTC_2578	7,8-dihydroxyflavone	3.42	64.4 µM	4.26E-4		55	3.17E-4
20	SGTC_1633	st005664	3.34	67.9 µM	5.56E-4		61	4.20E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.107	4.85E-10	YDR032C	PST2	hom	Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.098	1.31E-8	YJL120W_d	YJL120W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
0.097	2.06E-8	YMR075C-A_d	YMR075C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W
0.093	7.44E-8	YFR031C-A	RPL2A	hom	Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
0.091	1.28E-7	YPL080C_d	YPL080C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.089	2.34E-7	YBL105C	PKC1	het	Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
0.089	2.84E-7	YLL045C	RPL8B	hom	Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
0.087	5.28E-7	YJL188C_d	BUD19_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.086	6.56E-7	YPR187W	RPO26	het	RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
0.082	1.95E-6	YPR074C	TKL1	hom	Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
0.081	2.40E-6	YIL043C	CBR1	hom	Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
0.080	3.88E-6	YJL176C	SWI3	hom	Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
0.079	4.83E-6	YDL217C	TIM22	het	Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
0.078	5.69E-6	YDL082W	RPL13A	hom	Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
0.078	6.44E-6	YIL143C	SSL2	het	Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3