HIPHOP chemogenomics database

YHR187W / IKI1

Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR187W / IKI1

Click on Significant Values for Screen Details

Top fitness defect scores for YHR187W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1844	rapamycin	18.60	4.0 nM	1.18E-73		6	9.20E-78
2	SGTC_2679	rapamycin	15.70	1.0 nM	6.39E-53		6	8.27E-56
3	SGTC_1754	st042065	8.48	16.1 µM	7.18E-17	NEO1-PIK1	50	1.13E-17
4	SGTC_686	1319-0111	7.56	510.0 µM	8.70E-14	60S ribosome export	1	2.04E-14
5	SGTC_1803	st050068	6.78	52.4 µM	1.87E-11	NEO1-PIK1	60	5.90E-12
6	SGTC_1818	st052136	6.12	56.3 µM	1.16E-9	NEO1-PIK1	12	4.61E-10
7	SGTC_2873	9040596	5.99	71.4 µM	2.55E-9		14	1.06E-9
8	SGTC_2257	7960413	5.61	200.0 µM	2.15E-8	NEO1-PIK1	19	9.98E-9
9	SGTC_1868	st057644	5.59	42.7 µM	2.40E-8		12	1.12E-8
10	SGTC_1800	st050079	5.58	29.1 µM	2.54E-8	NEO1-PIK1	52	1.19E-8
11	SGTC_679	0422-0091	5.54	41.4 µM	3.23E-8	Golgi	9	1.53E-8
12	SGTC_1945	st075991	5.32	56.6 µM	1.01E-7	60S ribosome export	4	5.09E-8
13	SGTC_3202	9113761	5.31	49.5 µM	1.07E-7		17	5.43E-8
14	SGTC_42	0133-0086	5.24	60.6 µM	1.55E-7		6	8.01E-8
15	SGTC_2325	6158266	5.15	200.0 µM	2.42E-7		9	1.28E-7
16	SGTC_704	1310-0270	5.09	142.0 µM	3.34E-7		13	1.79E-7
17	SGTC_685	1278-0033	4.60	54.9 µM	3.49E-6	60S ribosome export	12	2.12E-6
18	SGTC_37	0566-0070	4.55	1.6 µM	4.29E-6		17	2.63E-6
19	SGTC_2327	6189656	4.50	200.0 µM	5.42E-6		14	3.37E-6
20	SGTC_629	3448-6466	4.49	76.7 µM	5.77E-6		8	3.60E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.406	4.90E-133	YGR200C	ELP2	hom	Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
0.403	2.37E-131	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.366	7.35E-107	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.352	1.44E-98	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.351	6.10E-98	YGR163W	GTR2	hom	Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.340	2.21E-91	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.328	6.25E-85	YLR384C	IKI3	hom	Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.323	1.76E-82	YKL110C	KTI12	hom	Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.318	9.41E-80	YHR186C	KOG1	het	Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.316	2.47E-78	YNL119W	NCS2	hom	Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.307	2.10E-74	YGL211W	NCS6	hom	Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.285	7.85E-64	YOR184W	SER1	hom	3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
0.265	8.90E-55	YHR111W	UBA4	hom	Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
0.255	8.24E-51	YIL008W	URM1	hom	Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
0.253	3.85E-50	YPL202C	AFT2	hom	Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication