YHR200W / RPN10

Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR200W / RPN10

Click on Significant Values for Screen Details ID:SGTC_105|Compound:0111-0055|FD-Score:3.08|P-value:9.68E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.52|P-value:1.69E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.52|P-value:1.97E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.29|P-value:4.69E-4 ID:SGTC_433|Compound:pimozide|FD-Score:4.27|P-value:8.47E-6 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.95|P-value:3.54E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.18|P-value:6.97E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.53|P-value:1.87E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.21|P-value:6.30E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:4.50|P-value:2.92E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:3.34|P-value:3.87E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.32|P-value:4.11E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.53|P-value:2.54E-6 ID:SGTC_929|Compound:2805-0021|FD-Score:3.40|P-value:3.06E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:3.26|P-value:5.15E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.47|P-value:2.05E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.66|P-value:1.00E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.51|P-value:2.06E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.63|P-value:1.31E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.49|P-value:3.06E-6 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.72|P-value:9.00E-5 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.25|P-value:5.39E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.60|P-value:1.43E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.12|P-value:8.48E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.19|P-value:6.73E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.38|P-value:3.31E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.15|P-value:7.55E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.34|P-value:3.86E-4 ID:SGTC_1793|Compound:st048256|FD-Score:3.33|P-value:4.00E-4 ID:SGTC_1847|Compound:st056231|FD-Score:3.20|P-value:6.34E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.78|P-value:7.10E-5 ID:SGTC_1923|Compound:st058458|FD-Score:3.65|P-value:1.21E-4 ID:SGTC_2198|Compound:6589944|FD-Score:5.50|P-value:1.50E-8 ID:SGTC_2297|Compound:7971671|FD-Score:3.31|P-value:4.40E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-4.53|P-value:2.09E-6 ID:SGTC_2424|Compound:5537928|FD-Score:3.16|P-value:7.48E-4 ID:SGTC_2442|Compound:5158906|FD-Score:4.64|P-value:1.42E-6 ID:SGTC_2513|Compound:baicalein|FD-Score:4.07|P-value:2.10E-5 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.25|P-value:5.33E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.70|P-value:1.10E-6 ID:SGTC_2549|Compound:cedrol|FD-Score:3.70|P-value:9.90E-5 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.74|P-value:8.39E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:8.65|P-value:1.22E-18 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.72|P-value:8.95E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:4.05|P-value:2.25E-5 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.32|P-value:4.18E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.20|P-value:6.34E-4 ID:SGTC_2860|Compound:9025725|FD-Score:-3.53|P-value:1.66E-4 ID:SGTC_2862|Compound:9027634|FD-Score:6.28|P-value:1.17E-10 ID:SGTC_2879|Compound:9041815|FD-Score:-3.04|P-value:9.96E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.77|P-value:7.30E-5 ID:SGTC_2954|Compound:9074818|FD-Score:5.37|P-value:3.04E-8 ID:SGTC_2964|Compound:9083345|FD-Score:-3.25|P-value:4.80E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.16|P-value:7.50E-4 ID:SGTC_3065|Compound:9113888|FD-Score:-3.49|P-value:1.95E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.28|P-value:4.78E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.66|P-value:1.14E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.37|P-value:3.03E-4 ID:SGTC_3203|Compound:9113543|FD-Score:4.74|P-value:8.73E-7 ID:SGTC_3205|Compound:9113483|FD-Score:3.13|P-value:8.14E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.23|P-value:5.70E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.43|P-value:2.74E-4 ID:SGTC_3277|Compound:9138724|FD-Score:3.65|P-value:1.21E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:3.08|P-value:9.68E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:-3.52|P-value:1.69E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.52|P-value:1.97E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.29|P-value:4.69E-4 ID:SGTC_433|Compound:pimozide|FD-Score:4.27|P-value:8.47E-6 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.95|P-value:3.54E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.18|P-value:6.97E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.53|P-value:1.87E-4 ID:SGTC_629|Compound:3448-6466|FD-Score:3.21|P-value:6.30E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:4.50|P-value:2.92E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:3.34|P-value:3.87E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.32|P-value:4.11E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.53|P-value:2.54E-6 ID:SGTC_929|Compound:2805-0021|FD-Score:3.40|P-value:3.06E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:3.26|P-value:5.15E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:-3.47|P-value:2.05E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-3.66|P-value:1.00E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.51|P-value:2.06E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.63|P-value:1.31E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:4.49|P-value:3.06E-6 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.72|P-value:9.00E-5 ID:SGTC_1279|Compound:0874-0590|FD-Score:3.25|P-value:5.39E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.60|P-value:1.43E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.12|P-value:8.48E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:3.19|P-value:6.73E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.38|P-value:3.31E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.15|P-value:7.55E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.34|P-value:3.86E-4 ID:SGTC_1793|Compound:st048256|FD-Score:3.33|P-value:4.00E-4 ID:SGTC_1847|Compound:st056231|FD-Score:3.20|P-value:6.34E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.78|P-value:7.10E-5 ID:SGTC_1923|Compound:st058458|FD-Score:3.65|P-value:1.21E-4 ID:SGTC_2198|Compound:6589944|FD-Score:5.50|P-value:1.50E-8 ID:SGTC_2297|Compound:7971671|FD-Score:3.31|P-value:4.40E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-4.53|P-value:2.09E-6 ID:SGTC_2424|Compound:5537928|FD-Score:3.16|P-value:7.48E-4 ID:SGTC_2442|Compound:5158906|FD-Score:4.64|P-value:1.42E-6 ID:SGTC_2513|Compound:baicalein|FD-Score:4.07|P-value:2.10E-5 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.25|P-value:5.33E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.70|P-value:1.10E-6 ID:SGTC_2549|Compound:cedrol|FD-Score:3.70|P-value:9.90E-5 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:3.74|P-value:8.39E-5 ID:SGTC_2561|Compound:acetosyringone|FD-Score:8.65|P-value:1.22E-18 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.72|P-value:8.95E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:4.05|P-value:2.25E-5 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.32|P-value:4.18E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.20|P-value:6.34E-4 ID:SGTC_2860|Compound:9025725|FD-Score:-3.53|P-value:1.66E-4 ID:SGTC_2862|Compound:9027634|FD-Score:6.28|P-value:1.17E-10 ID:SGTC_2879|Compound:9041815|FD-Score:-3.04|P-value:9.96E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.77|P-value:7.30E-5 ID:SGTC_2954|Compound:9074818|FD-Score:5.37|P-value:3.04E-8 ID:SGTC_2964|Compound:9083345|FD-Score:-3.25|P-value:4.80E-4 ID:SGTC_3031|Compound:9088762|FD-Score:3.16|P-value:7.50E-4 ID:SGTC_3065|Compound:9113888|FD-Score:-3.49|P-value:1.95E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.28|P-value:4.78E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.66|P-value:1.14E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.37|P-value:3.03E-4 ID:SGTC_3203|Compound:9113543|FD-Score:4.74|P-value:8.73E-7 ID:SGTC_3205|Compound:9113483|FD-Score:3.13|P-value:8.14E-4 ID:SGTC_3215|Compound:9129942|FD-Score:3.23|P-value:5.70E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.43|P-value:2.74E-4 ID:SGTC_3277|Compound:9138724|FD-Score:3.65|P-value:1.21E-4

Top fitness defect scores for YHR200W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2561 acetosyringone 8.65 100.0 µM 1.22E-18 1 2.56E-18
2 SGTC_2862 9027634 6.28 71.4 µM 1.17E-10 ERG2 9 1.68E-10
3 SGTC_2198 6589944 5.50 200.0 µM 1.50E-8 8 1.95E-8
4 SGTC_2954 9074818 5.37 52.7 µM 3.04E-8 16 3.91E-8
5 SGTC_3203 9113543 4.74 49.5 µM 8.73E-7 14 1.05E-6
6 SGTC_2536 dehydroabietamide 4.70 32.0 µM 1.10E-6 11 1.32E-6
7 SGTC_2442 5158906 4.64 48.5 µM 1.42E-6 10 1.70E-6
8 SGTC_916 4466-0038 4.53 42.0 nM 2.54E-6 cell wall 47 3.00E-6
9 SGTC_711 k292-0785 4.50 179.0 µM 2.92E-6 DNA intercalators 13 3.45E-6
10 SGTC_1098 clofazimine 4.49 15.1 µM 3.06E-6 heme biosynthesis & mitochondrial translocase 14 3.61E-6
11 SGTC_433 pimozide 4.27 108.0 µM 8.47E-6 13 9.80E-6
12 SGTC_2513 baicalein 4.07 25.6 µM 2.10E-5 calcium & mitochondrial duress 24 2.39E-5
13 SGTC_2610 ononetin 4.05 44.2 µM 2.25E-5 40 2.55E-5
14 SGTC_491 tetrandrine 3.95 80.3 µM 3.54E-5 11 3.99E-5
15 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.78 14.9 µM 7.10E-5 15 7.91E-5
16 SGTC_2882 9058044 3.77 58.4 µM 7.30E-5 21 8.12E-5
17 SGTC_2558 carylophyllene oxide 3.74 100.0 µM 8.39E-5 17 9.31E-5
18 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 3.72 15.3 µM 8.95E-5 41 9.92E-5
19 SGTC_1246 0422-0085 3.72 105.0 µM 9.00E-5 39 9.98E-5
20 SGTC_2549 cedrol 3.70 20.9 µM 9.90E-5 19 1.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.69E-9 YPL009C TAE2 hom Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.092 8.11E-8 YCR024C-A PMP1 hom Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
0.087 5.14E-7 YOR102W_d YOR102W_d het Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
0.085 8.17E-7 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.084 9.75E-7 YOR197W MCA1 hom Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization
0.082 2.17E-6 YPL099C AIM43 hom Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
0.081 2.92E-6 YPL250C ICY2 hom Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate
0.080 3.73E-6 YOR384W FRE5 hom Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.079 4.58E-6 YLR212C TUB4 het Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.075 1.23E-5 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.075 1.42E-5 YNL126W SPC98 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.073 2.08E-5 YJR024C MDE1 hom 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
0.073 2.41E-5 YIR009W MSL1 hom U2B component of U2 snRNP, involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
0.072 2.83E-5 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.071 3.81E-5 YOR195W SLK19 hom Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate