HIPHOP chemogenomics database

YIL007C / NAS2

Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YIL007C / NAS2

Click on Significant Values for Screen Details

Top fitness defect scores for YIL007C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_147	4466-0538	6.22	29.2 µM	9.89E-12	ergosterol biosynthesis	3	2.50E-10
2	SGTC_2063	5246145	5.39	200.0 µM	3.01E-9		8	3.58E-8
3	SGTC_2598	perillyl alcohol	4.61	100.0 µM	3.12E-7		17	2.01E-6
4	SGTC_1616	st002734	4.59	56.1 µM	3.50E-7		13	2.23E-6
5	SGTC_1444	4112-3581	4.12	28.0 µM	4.04E-6		7	1.86E-5
6	SGTC_876	0720-0963	3.91	56.8 µM	1.17E-5		16	4.68E-5
7	SGTC_2179	6298986	3.78	200.0 µM	2.09E-5	heme requiring	20	7.75E-5
8	SGTC_1644	st010134	3.68	20.9 µM	3.34E-5	mitochondrial processes	14	1.17E-4
9	SGTC_1146	3579-1023	3.66	7.0 µM	3.59E-5		23	1.24E-4
10	SGTC_1155	4088-0394	3.49	48.2 µM	7.68E-5		48	2.41E-4
11	SGTC_2284	7945075	3.43	39.2 µM	9.87E-5	mitochondrial processes	43	2.99E-4
12	SGTC_2133	5320837	3.36	200.0 µM	1.34E-4	ERG2	48	3.89E-4
13	SGTC_369	0957-0393	3.33	4.5 µM	1.50E-4	cell wall signaling	52	4.31E-4
14	SGTC_3276	9139060	3.32	49.5 µM	1.57E-4	60S ribosome export	14	4.48E-4
15	SGTC_68	0986-0284	3.30	82.8 µM	1.72E-4		105	4.85E-4
16	SGTC_1242	0402-0103	3.27	60.1 µM	1.94E-4		49	5.37E-4
17	SGTC_2559	tetrahydrosappanone a trimethyl ether	3.22	100.0 µM	2.34E-4		32	6.33E-4
18	SGTC_796	1234-0020	3.18	70.7 µM	2.75E-4	SWF1 & branched chain AA biosynthesis	49	7.28E-4
19	SGTC_2534	helenine	3.18	20.1 µM	2.83E-4	RSC complex & mRNA processing	31	7.47E-4
20	SGTC_78	0336-0123	3.16	156.8 µM	2.99E-4		45	7.83E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.121	1.87E-12	YIR013C	GAT4	hom	Protein containing GATA family zinc finger motifs
0.110	1.64E-10	YGL248W	PDE1	hom	Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
0.102	2.72E-9	YDR329C	PEX3	hom	Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p
0.082	2.00E-6	YIL023C	YKE4	hom	Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family
0.081	2.43E-6	YHR085W	IPI1	het	Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
0.080	3.20E-6	YJL208C	NUC1	hom	Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy
0.079	5.00E-6	YLL019C	KNS1	hom	Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
0.077	7.01E-6	YER089C	PTC2	hom	Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
0.075	1.46E-5	YLR249W	YEF3	het	Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
0.074	1.80E-5	YNR025C_d	YNR025C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
0.072	2.75E-5	YLR403W	SFP1	hom	Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
0.071	4.00E-5	YMR287C	DSS1	hom	3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.071	4.16E-5	YER039C	HVG1	hom	Protein of unknown function, has homology to Vrg4p
0.070	4.85E-5	YER020W	GPA2	hom	Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.068	7.31E-5	YPR041W	TIF5	het	Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2