YIL042C / PKP1

Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL042C / PKP1

Click on Significant Values for Screen Details ID:SGTC_144|Compound:4161-2749|FD-Score:-2.99|P-value:3.62E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.65|P-value:1.86E-5 ID:SGTC_289|Compound:k017-0060|FD-Score:-3.03|P-value:3.08E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.19|P-value:1.52E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.88|P-value:5.51E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.87|P-value:5.98E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:-3.46|P-value:4.47E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:3.08|P-value:2.84E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-4.28|P-value:6.45E-7 ID:SGTC_1374|Compound:2889-5571|FD-Score:-2.94|P-value:4.33E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.23|P-value:8.92E-7 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.32|P-value:8.69E-5 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-2.78|P-value:8.30E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.39|P-value:7.37E-5 ID:SGTC_2014|Compound:4013930|FD-Score:3.30|P-value:1.09E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.01|P-value:3.21E-4 ID:SGTC_2404|Compound:5161160|FD-Score:2.88|P-value:6.32E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-2.82|P-value:6.93E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.65|P-value:7.49E-8 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.68|P-value:1.87E-5 ID:SGTC_2630|Compound:antimycin a|FD-Score:-3.54|P-value:3.06E-5 ID:SGTC_2641|Compound:levomentholum|FD-Score:2.81|P-value:8.26E-4 ID:SGTC_2659|Compound:sanguinarine|FD-Score:-3.05|P-value:2.82E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.01|P-value:3.28E-4 ID:SGTC_2825|Compound:7998094|FD-Score:-2.85|P-value:6.16E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-2.89|P-value:5.25E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.64|P-value:1.96E-5 ID:SGTC_2925|Compound:9010536|FD-Score:3.35|P-value:8.68E-5 ID:SGTC_2927|Compound:9011802|FD-Score:-3.09|P-value:2.30E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.60|P-value:2.68E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-2.90|P-value:5.13E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.99|P-value:3.62E-4 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.65|P-value:1.86E-5 ID:SGTC_289|Compound:k017-0060|FD-Score:-3.03|P-value:3.08E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-3.19|P-value:1.52E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-2.88|P-value:5.51E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.87|P-value:5.98E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:-3.46|P-value:4.47E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:3.08|P-value:2.84E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:-4.28|P-value:6.45E-7 ID:SGTC_1374|Compound:2889-5571|FD-Score:-2.94|P-value:4.33E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.23|P-value:8.92E-7 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:-3.32|P-value:8.69E-5 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-2.78|P-value:8.30E-4 ID:SGTC_1976|Compound:st070609|FD-Score:3.39|P-value:7.37E-5 ID:SGTC_2014|Compound:4013930|FD-Score:3.30|P-value:1.09E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.01|P-value:3.21E-4 ID:SGTC_2404|Compound:5161160|FD-Score:2.88|P-value:6.32E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:-2.82|P-value:6.93E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.65|P-value:7.49E-8 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:3.68|P-value:1.87E-5 ID:SGTC_2630|Compound:antimycin a|FD-Score:-3.54|P-value:3.06E-5 ID:SGTC_2641|Compound:levomentholum|FD-Score:2.81|P-value:8.26E-4 ID:SGTC_2659|Compound:sanguinarine|FD-Score:-3.05|P-value:2.82E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.01|P-value:3.28E-4 ID:SGTC_2825|Compound:7998094|FD-Score:-2.85|P-value:6.16E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-2.89|P-value:5.25E-4 ID:SGTC_2840|Compound:9008771|FD-Score:-3.64|P-value:1.96E-5 ID:SGTC_2925|Compound:9010536|FD-Score:3.35|P-value:8.68E-5 ID:SGTC_2927|Compound:9011802|FD-Score:-3.09|P-value:2.30E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.60|P-value:2.68E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-2.90|P-value:5.13E-4

Top fitness defect scores for YIL042C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 3.68 100.0 µM 1.87E-5 60S ribosome export 28 1.18E-4
2 SGTC_2930 9013230 3.60 14.9 µM 2.68E-5 heme biosynthesis & mitochondrial translocase 52 1.57E-4
3 SGTC_1976 st070609 3.39 63.5 µM 7.37E-5 26 3.53E-4
4 SGTC_2925 9010536 3.35 32.5 µM 8.68E-5 azole & statin 76 4.03E-4
5 SGTC_2014 4013930 3.30 200.0 µM 1.09E-4 67 4.86E-4
6 SGTC_940 3232-1287 3.08 54.8 µM 2.84E-4 NEO1 79 0.00104
7 SGTC_2404 5161160 2.88 200.0 µM 6.32E-4 73 0.00199
8 SGTC_2641 levomentholum 2.81 100.0 µM 8.26E-4 28 0.00247
9 SGTC_2645 abamectin 2.73 100.0 µM 0.00114 heme requiring 85 0.00320
10 SGTC_2643 anethole 2.66 100.0 µM 0.00147 88 0.00393
11 SGTC_411 enrofloxacin 2.63 100.0 µM 0.00165 DNA damage response 67 0.00432
12 SGTC_2516 gossypol 2.62 71.5 µM 0.00167 88 0.00436
13 SGTC_942 3253-1115 2.57 227.0 µM 0.00199 108 0.00503
14 SGTC_533 1222-0222 2.52 6.7 µM 0.00243 PDR1 120 0.00592
15 SGTC_2394 5221648 2.50 200.0 µM 0.00261 132 0.00627
16 SGTC_1784 st039334 2.49 36.3 µM 0.00266 115 0.00637
17 SGTC_306 1672-3436 2.48 11.6 µM 0.00272 Golgi 106 0.00649
18 SGTC_1975 st070967 2.47 30.8 µM 0.00284 158 0.00671
19 SGTC_2591 skatole 2.46 100.0 µM 0.00301 131 0.00704
20 SGTC_1452 4194-0072 2.44 190.0 µM 0.00318 122 0.00736

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.242 5.46E-46 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.210 1.36E-34 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.209 2.21E-34 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.196 2.05E-30 YML108W YML108W hom Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.191 5.50E-29 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.182 2.93E-26 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.181 5.42E-26 YLR367W RPS22B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.168 9.52E-23 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene
0.165 6.00E-22 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.164 1.50E-21 YDR528W HLR1 hom Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication
0.150 2.09E-18 YNL339C YRF1-6 hom Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.150 2.42E-18 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.147 1.20E-17 YNR044W AGA1 hom Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
0.146 1.55E-17 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.146 2.18E-17 YFR017C IGD1 hom Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication