HIPHOP chemogenomics database

YIL042C / PKP1

Mitochondrial protein kinase involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p

Zygosity: Homozygous strain

fixedexpanded
Profile for YIL042C / PKP1

Click on Significant Values for Screen Details

Top fitness defect scores for YIL042C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2590	3,4',5',6',7'-pentamethoxyflavone	3.68	100.0 µM	1.87E-5	60S ribosome export	28	1.18E-4
2	SGTC_2930	9013230	3.60	14.9 µM	2.68E-5	heme biosynthesis & mitochondrial translocase	52	1.57E-4
3	SGTC_1976	st070609	3.39	63.5 µM	7.37E-5		26	3.53E-4
4	SGTC_2925	9010536	3.35	32.5 µM	8.68E-5	azole & statin	76	4.03E-4
5	SGTC_2014	4013930	3.30	200.0 µM	1.09E-4		67	4.86E-4
6	SGTC_940	3232-1287	3.08	54.8 µM	2.84E-4	NEO1	79	0.00104
7	SGTC_2404	5161160	2.88	200.0 µM	6.32E-4		73	0.00199
8	SGTC_2641	levomentholum	2.81	100.0 µM	8.26E-4		28	0.00247
9	SGTC_2645	abamectin	2.73	100.0 µM	0.00114	heme requiring	85	0.00320
10	SGTC_2643	anethole	2.66	100.0 µM	0.00147		88	0.00393
11	SGTC_411	enrofloxacin	2.63	100.0 µM	0.00165	DNA damage response	67	0.00432
12	SGTC_2516	gossypol	2.62	71.5 µM	0.00167		88	0.00436
13	SGTC_942	3253-1115	2.57	227.0 µM	0.00199		108	0.00503
14	SGTC_533	1222-0222	2.52	6.7 µM	0.00243	PDR1	120	0.00592
15	SGTC_2394	5221648	2.50	200.0 µM	0.00261		132	0.00627
16	SGTC_1784	st039334	2.49	36.3 µM	0.00266		115	0.00637
17	SGTC_306	1672-3436	2.48	11.6 µM	0.00272	Golgi	106	0.00649
18	SGTC_1975	st070967	2.47	30.8 µM	0.00284		158	0.00671
19	SGTC_2591	skatole	2.46	100.0 µM	0.00301		131	0.00704
20	SGTC_1452	4194-0072	2.44	190.0 µM	0.00318		122	0.00736

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.242	5.46E-46	YDR169C	STB3	hom	Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.210	1.36E-34	YDR521W_d	YDR521W_d	hom	Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.209	2.21E-34	YBL017C	PEP1	hom	Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.196	2.05E-30	YML108W	YML108W	hom	Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
0.191	5.50E-29	YDL176W	YDL176W	hom	Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.182	2.93E-26	YFL014W	HSP12	hom	Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.181	5.42E-26	YLR367W	RPS22B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
0.168	9.52E-23	YOR152C_p	YOR152C_p	hom	Putative protein of unknown function; YOR152C is not an essential gene
0.165	6.00E-22	YGR159C	NSR1	hom	Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.164	1.50E-21	YDR528W	HLR1	hom	Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; HLR1 has a paralog, LRE1, that arose from the whole genome duplication
0.150	2.09E-18	YNL339C	YRF1-6	hom	Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
0.150	2.42E-18	YGR022C_d	YGR022C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.147	1.20E-17	YNR044W	AGA1	hom	Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
0.146	1.55E-17	YNL067W	RPL9B	hom	Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.146	2.18E-17	YFR017C	IGD1	hom	Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication