YIL068C / SEC6

Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YIL068C / SEC6

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:11.00|P-value:2.72E-29 ID:SGTC_81|Compound:0411-0046|FD-Score:4.96|P-value:2.38E-7 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.94|P-value:1.92E-7 ID:SGTC_131|Compound:0141-0277|FD-Score:3.55|P-value:1.63E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.07|P-value:1.84E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.41|P-value:1.39E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:5.80|P-value:1.84E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:3.40|P-value:2.82E-4 ID:SGTC_252|Compound:miconazole|FD-Score:8.15|P-value:5.45E-17 ID:SGTC_266|Compound:formosanin C|FD-Score:6.89|P-value:1.20E-12 ID:SGTC_309|Compound:k408-0051|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:14.90|P-value:2.36E-52 ID:SGTC_320|Compound:4092-0846|FD-Score:9.90|P-value:3.11E-24 ID:SGTC_330|Compound:1488-0319|FD-Score:-3.37|P-value:2.53E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:4.12|P-value:1.48E-5 ID:SGTC_459|Compound:nigericin|FD-Score:3.61|P-value:1.26E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.16|P-value:6.93E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.17|P-value:5.30E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.27|P-value:4.63E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:-3.45|P-value:1.91E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.07|P-value:7.60E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.17|P-value:6.64E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.62|P-value:1.36E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.50|P-value:1.15E-8 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.01|P-value:2.41E-5 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.46|P-value:1.84E-4 ID:SGTC_1727|Compound:st034309|FD-Score:-5.26|P-value:3.31E-8 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.31|P-value:3.97E-4 ID:SGTC_2084|Compound:5306078|FD-Score:-3.01|P-value:9.32E-4 ID:SGTC_2240|Compound:6690706|FD-Score:3.14|P-value:7.39E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.81|P-value:5.14E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.56|P-value:1.81E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:5.08|P-value:1.21E-7 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.74|P-value:7.52E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:11.90|P-value:3.36E-34 ID:SGTC_2778|Compound:tioconazole|FD-Score:10.60|P-value:2.61E-27 ID:SGTC_2793|Compound:7325131|FD-Score:5.67|P-value:4.01E-9 ID:SGTC_2797|Compound:7859098|FD-Score:-3.13|P-value:6.22E-4 ID:SGTC_2799|Compound:7812892|FD-Score:4.41|P-value:3.77E-6 ID:SGTC_2801|Compound:7907800|FD-Score:3.35|P-value:3.45E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.56|P-value:1.53E-4 ID:SGTC_2920|Compound:7982904|FD-Score:5.41|P-value:1.93E-8 ID:SGTC_2944|Compound:9067346|FD-Score:3.17|P-value:6.70E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.23|P-value:4.39E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.62|P-value:9.43E-5 ID:SGTC_3034|Compound:9090421|FD-Score:-3.22|P-value:4.51E-4 ID:SGTC_3108|Compound:9121785|FD-Score:4.04|P-value:2.04E-5 ID:SGTC_3243|Compound:9134147|FD-Score:4.15|P-value:1.28E-5 ID:SGTC_3346|Compound:9158254|FD-Score:-3.17|P-value:5.31E-4 ID:SGTC_57|Compound:miconazole|FD-Score:11.00|P-value:2.72E-29 ID:SGTC_81|Compound:0411-0046|FD-Score:4.96|P-value:2.38E-7 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.94|P-value:1.92E-7 ID:SGTC_131|Compound:0141-0277|FD-Score:3.55|P-value:1.63E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.07|P-value:1.84E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-5.41|P-value:1.39E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:5.80|P-value:1.84E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:3.40|P-value:2.82E-4 ID:SGTC_252|Compound:miconazole|FD-Score:8.15|P-value:5.45E-17 ID:SGTC_266|Compound:formosanin C|FD-Score:6.89|P-value:1.20E-12 ID:SGTC_309|Compound:k408-0051|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:14.90|P-value:2.36E-52 ID:SGTC_320|Compound:4092-0846|FD-Score:9.90|P-value:3.11E-24 ID:SGTC_330|Compound:1488-0319|FD-Score:-3.37|P-value:2.53E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:4.12|P-value:1.48E-5 ID:SGTC_459|Compound:nigericin|FD-Score:3.61|P-value:1.26E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.16|P-value:6.93E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.17|P-value:5.30E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.27|P-value:4.63E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:-3.45|P-value:1.91E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.07|P-value:7.60E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:3.17|P-value:6.64E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:4.62|P-value:1.36E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.50|P-value:1.15E-8 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.01|P-value:2.41E-5 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.46|P-value:1.84E-4 ID:SGTC_1727|Compound:st034309|FD-Score:-5.26|P-value:3.31E-8 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.31|P-value:3.97E-4 ID:SGTC_2084|Compound:5306078|FD-Score:-3.01|P-value:9.32E-4 ID:SGTC_2240|Compound:6690706|FD-Score:3.14|P-value:7.39E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.81|P-value:5.14E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.56|P-value:1.81E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:5.08|P-value:1.21E-7 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:3.74|P-value:7.52E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:11.90|P-value:3.36E-34 ID:SGTC_2778|Compound:tioconazole|FD-Score:10.60|P-value:2.61E-27 ID:SGTC_2793|Compound:7325131|FD-Score:5.67|P-value:4.01E-9 ID:SGTC_2797|Compound:7859098|FD-Score:-3.13|P-value:6.22E-4 ID:SGTC_2799|Compound:7812892|FD-Score:4.41|P-value:3.77E-6 ID:SGTC_2801|Compound:7907800|FD-Score:3.35|P-value:3.45E-4 ID:SGTC_2919|Compound:7978188|FD-Score:3.56|P-value:1.53E-4 ID:SGTC_2920|Compound:7982904|FD-Score:5.41|P-value:1.93E-8 ID:SGTC_2944|Compound:9067346|FD-Score:3.17|P-value:6.70E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.23|P-value:4.39E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.62|P-value:9.43E-5 ID:SGTC_3034|Compound:9090421|FD-Score:-3.22|P-value:4.51E-4 ID:SGTC_3108|Compound:9121785|FD-Score:4.04|P-value:2.04E-5 ID:SGTC_3243|Compound:9134147|FD-Score:4.15|P-value:1.28E-5 ID:SGTC_3346|Compound:9158254|FD-Score:-3.17|P-value:5.31E-4

Top fitness defect scores for YIL068C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_312 4092-0653 14.90 6.8 µM 2.36E-52 ergosterol depletion effects on membrane 2 2.11E-50 1.98
2 SGTC_2651 1327-0036 11.90 5.0 µM 3.36E-34 11 5.58E-33 0.17
3 SGTC_57 miconazole 11.00 162.5 nM 2.72E-29 ergosterol depletion effects on membrane 4 2.88E-28 0.58
4 SGTC_2778 tioconazole 10.60 65.1 nM 2.61E-27 ergosterol depletion effects on membrane 3 2.30E-26 1.28
5 SGTC_320 4092-0846 9.90 5.3 µM 3.11E-24 ergosterol depletion effects on membrane 5 2.07E-23 2.77
6 SGTC_252 miconazole 8.15 1.3 nM 5.45E-17 ergosterol depletion effects on membrane 6 1.89E-16 2.24
7 SGTC_266 formosanin C 6.89 406.0 nM 1.20E-12 sphingolipid biosynthesis & PDR1 5 2.84E-12 0.43
8 SGTC_163 1327-0036 5.80 7.5 µM 1.84E-9 34 3.31E-9 0.02
9 SGTC_2793 7325131 5.67 81.8 µM 4.01E-9 1 7.02E-9 0.75
10 SGTC_1072 oxiconazole 5.50 14.9 nM 1.15E-8 ergosterol depletion effects on membrane 12 1.94E-8 0.16
11 SGTC_2920 7982904 5.41 15.8 µM 1.93E-8 4 3.18E-8 0.09
12 SGTC_2594 a-mangostin 5.08 100.0 µM 1.21E-7 3 1.87E-7 0.90
13 SGTC_81 0411-0046 4.96 10.0 µM 2.38E-7 28 3.58E-7 0.11
14 SGTC_2249 haloperidol 4.81 116.4 µM 5.14E-7 ergosterol depletion effects on membrane 25 7.54E-7 0.14
15 SGTC_1029 k015-0030 4.62 64.7 µM 1.36E-6 3 1.93E-6 0.33
16 SGTC_2567 aureobasidin a 4.56 60.0 nM 1.81E-6 36 2.54E-6 0.17
17 SGTC_2799 7812892 4.41 81.8 µM 3.77E-6 superoxide 14 5.15E-6 0.34
18 SGTC_3243 9134147 4.15 49.5 µM 1.28E-5 3 1.67E-5 0.53
19 SGTC_432 NS 1619 4.12 61.3 µM 1.48E-5 8 1.93E-5 0.10
20 SGTC_137 0438-0332 4.07 85.4 µM 1.84E-5 ubiquinone biosynthesis & proteasome 40 2.38E-5 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.437 3.54E-156 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.427 1.66E-148 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.409 2.24E-135 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.408 8.67E-135 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.334 4.47E-88 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.314 8.53E-78 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.301 3.41E-71 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.300 1.74E-70 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.294 9.61E-68 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.286 4.03E-64 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.282 1.68E-62 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.277 2.84E-60 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.273 2.14E-58 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.269 1.08E-56 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.259 1.61E-52 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria