HIPHOP chemogenomics database

YIL103W / DPH1

Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p

Zygosity: Homozygous strain

fixedexpanded
Profile for YIL103W / DPH1

Click on Significant Values for Screen Details

Top fitness defect scores for YIL103W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_955	1216-0110	8.56	33.0 µM	2.77E-16	sphingolipid biosynthesis & PDR1	18	5.68E-18
2	SGTC_2567	aureobasidin a	6.16	60.0 nM	2.54E-9		41	3.61E-10
3	SGTC_2283	7944793	6.02	200.0 µM	5.72E-9		7	8.95E-10
4	SGTC_838	0109-0203	5.95	71.4 µM	8.07E-9		1	1.31E-9
5	SGTC_859	0439-0102	5.95	54.6 µM	8.11E-9	redox potentiating	5	1.32E-9
6	SGTC_2541	muurolladie-3-one	5.93	83.1 µM	9.13E-9	excess fatty acid	5	1.51E-9
7	SGTC_2540	epiandrosterone	5.61	100.0 µM	4.90E-8		22	9.87E-9
8	SGTC_1785	bathophenanthroline	4.85	24.0 µM	1.99E-6	heme requiring	24	6.16E-7
9	SGTC_1844	rapamycin	4.85	4.0 nM	2.02E-6		119	6.25E-7
10	SGTC_1418	3966-3099	4.51	2.4 µM	8.90E-6		12	3.26E-6
11	SGTC_1474	0330-0125	4.21	5.3 µM	3.03E-5		25	1.28E-5
12	SGTC_422	cantharidin	4.17	155.0 µM	3.51E-5	cell wall signaling	50	1.50E-5
13	SGTC_3094	9117742	4.17	49.5 µM	3.59E-5		15	1.54E-5
14	SGTC_2439	5492030	4.12	7.2 µM	4.30E-5		8	1.88E-5
15	SGTC_66	0109-0187	4.11	42.4 µM	4.44E-5		34	1.95E-5
16	SGTC_2701	st077767	3.98	14.7 µM	7.29E-5		43	3.38E-5
17	SGTC_1941	st076221	3.92	61.8 µM	9.43E-5	sphingolipid biosynthesis & PDR1	82	4.50E-5
18	SGTC_969	1313-0238	3.83	134.0 µM	1.31E-4	mitochondrial processes	30	6.46E-5
19	SGTC_1426	4048-4480	3.83	71.7 µM	1.31E-4	60S ribosome export	20	6.49E-5
20	SGTC_3075	9116269	3.82	49.5 µM	1.33E-4		24	6.57E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.160	1.39E-20	YPL057C	SUR1	hom	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.146	1.60E-17	YKL004W	AUR1	het	Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.134	8.08E-15	YDR516C	EMI2	hom	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.132	1.49E-14	YGR143W	SKN1	hom	Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p
0.131	2.18E-14	YOR061W	CKA2	hom	Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
0.123	7.69E-13	YMR102C_p	YMR102C_p	hom	Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.121	2.09E-12	YOL001W	PHO80	hom	Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
0.120	3.01E-12	YDR326C	YSP2	hom	Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.117	9.13E-12	YGL251C	HFM1	hom	Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.115	2.05E-11	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.111	1.27E-10	YLR055C	SPT8	hom	Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.109	2.52E-10	YBR036C	CSG2	hom	Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
0.106	6.27E-10	YHR187W	IKI1	hom	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.106	8.73E-10	YMR211W	DML1	het	Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.105	9.44E-10	YGR209C	TRX2	hom	Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p; required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress