YIL119C / RPI1

Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL119C / RPI1

Click on Significant Values for Screen Details ID:SGTC_84|Compound:0443-0269|FD-Score:3.08|P-value:6.86E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.78|P-value:4.51E-5 ID:SGTC_433|Compound:pimozide|FD-Score:-3.21|P-value:5.79E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-3.84|P-value:4.95E-5 ID:SGTC_542|Compound:3381-0244|FD-Score:3.71|P-value:6.07E-5 ID:SGTC_552|Compound:k242-0005|FD-Score:3.23|P-value:3.92E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.30|P-value:3.09E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.78|P-value:4.46E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:3.46|P-value:1.65E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.04|P-value:7.79E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.45|P-value:1.74E-4 ID:SGTC_1426|Compound:4048-4480|FD-Score:3.34|P-value:2.62E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.53|P-value:1.27E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.02|P-value:8.62E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.66|P-value:7.25E-5 ID:SGTC_1697|Compound:st025554|FD-Score:3.20|P-value:4.40E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.90|P-value:2.71E-5 ID:SGTC_1769|Compound:st044406|FD-Score:3.00|P-value:9.16E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.85|P-value:4.59E-5 ID:SGTC_2296|Compound:7971656|FD-Score:-3.21|P-value:5.85E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.31|P-value:2.97E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-4.94|P-value:2.57E-7 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.32|P-value:2.86E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:4.25|P-value:5.42E-6 ID:SGTC_2523|Compound:spermine|FD-Score:3.22|P-value:4.17E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:4.36|P-value:3.25E-6 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.28|P-value:3.30E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.07|P-value:7.23E-4 ID:SGTC_2707|Compound:st078556|FD-Score:3.04|P-value:7.82E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.08|P-value:6.81E-4 ID:SGTC_2712|Compound:st079126|FD-Score:-3.95|P-value:2.97E-5 ID:SGTC_2717|Compound:st081029|FD-Score:3.35|P-value:2.51E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.75|P-value:6.64E-7 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.26|P-value:3.57E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.22|P-value:4.16E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-3.31|P-value:4.00E-4 ID:SGTC_84|Compound:0443-0269|FD-Score:3.08|P-value:6.86E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.78|P-value:4.51E-5 ID:SGTC_433|Compound:pimozide|FD-Score:-3.21|P-value:5.79E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-3.84|P-value:4.95E-5 ID:SGTC_542|Compound:3381-0244|FD-Score:3.71|P-value:6.07E-5 ID:SGTC_552|Compound:k242-0005|FD-Score:3.23|P-value:3.92E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.30|P-value:3.09E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:3.78|P-value:4.46E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:3.46|P-value:1.65E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.04|P-value:7.79E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.45|P-value:1.74E-4 ID:SGTC_1426|Compound:4048-4480|FD-Score:3.34|P-value:2.62E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.53|P-value:1.27E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.02|P-value:8.62E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.66|P-value:7.25E-5 ID:SGTC_1697|Compound:st025554|FD-Score:3.20|P-value:4.40E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.90|P-value:2.71E-5 ID:SGTC_1769|Compound:st044406|FD-Score:3.00|P-value:9.16E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:-3.85|P-value:4.59E-5 ID:SGTC_2296|Compound:7971656|FD-Score:-3.21|P-value:5.85E-4 ID:SGTC_2298|Compound:7976326|FD-Score:3.31|P-value:2.97E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-4.94|P-value:2.57E-7 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.32|P-value:2.86E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:4.25|P-value:5.42E-6 ID:SGTC_2523|Compound:spermine|FD-Score:3.22|P-value:4.17E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:4.36|P-value:3.25E-6 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.28|P-value:3.30E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:3.07|P-value:7.23E-4 ID:SGTC_2707|Compound:st078556|FD-Score:3.04|P-value:7.82E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.08|P-value:6.81E-4 ID:SGTC_2712|Compound:st079126|FD-Score:-3.95|P-value:2.97E-5 ID:SGTC_2717|Compound:st081029|FD-Score:3.35|P-value:2.51E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.75|P-value:6.64E-7 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.26|P-value:3.57E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.22|P-value:4.16E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-3.31|P-value:4.00E-4

Top fitness defect scores for YIL119C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2524 avocatin b 4.36 10.6 µM 3.25E-6 19 6.59E-6
2 SGTC_2518 prenyletin 4.25 46.3 µM 5.42E-6 8 1.07E-5
3 SGTC_1705 st027868 3.90 56.3 µM 2.71E-5 9 4.89E-5
4 SGTC_932 2857-0803 3.78 154.0 µM 4.46E-5 29 7.83E-5
5 SGTC_99 0109-0120 3.78 622.8 µM 4.51E-5 19 7.92E-5
6 SGTC_542 3381-0244 3.71 89.3 µM 6.07E-5 48 1.05E-4
7 SGTC_1635 st007035 3.66 42.4 µM 7.25E-5 230 1.24E-4
8 SGTC_1445 4239-0156 3.53 97.7 µM 1.27E-4 46 2.10E-4
9 SGTC_939 1165-0479 3.46 45.3 µM 1.65E-4 55 2.69E-4
10 SGTC_1033 k072-0102 3.45 21.3 µM 1.74E-4 60S ribosome export 39 2.84E-4
11 SGTC_2717 st081029 3.35 62.3 µM 2.51E-4 31 4.01E-4
12 SGTC_1426 4048-4480 3.34 71.7 µM 2.62E-4 60S ribosome export 34 4.17E-4
13 SGTC_2500 5-alpha-androstan-3,17-dione 3.32 61.4 µM 2.86E-4 60 4.52E-4
14 SGTC_2298 7976326 3.31 193.8 µM 2.97E-4 21 4.69E-4
15 SGTC_807 1315-0151 3.30 48.0 µM 3.09E-4 50 4.86E-4
16 SGTC_2573 2',4'-dihydroxychalcone 3.28 15.0 µM 3.30E-4 91 5.18E-4
17 SGTC_2764 methyldopa 3.26 94.7 µM 3.57E-4 44 5.58E-4
18 SGTC_552 k242-0005 3.23 476.0 µM 3.92E-4 41 6.09E-4
19 SGTC_3129 9127257 3.22 49.5 µM 4.16E-4 51 6.44E-4
20 SGTC_2523 spermine 3.22 47.7 µM 4.17E-4 calcium & mitochondrial duress 49 6.45E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.58E-18 YNR018W RCF2 hom Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.137 1.68E-15 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.136 2.81E-15 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.125 3.30E-13 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.122 1.20E-12 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.121 2.15E-12 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.115 2.41E-11 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.114 3.32E-11 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.112 8.08E-11 YKL155C RSM22 hom Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.104 1.30E-9 YPL189C-A COA2 hom Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
0.098 1.46E-8 YNR045W PET494 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
0.097 2.04E-8 YNL213C RRG9 hom Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.097 2.06E-8 YDL172C_d YDL172C_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.095 4.04E-8 YDL067C COX9 hom Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.094 5.70E-8 YNL073W MSK1 hom Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)