YIL132C / CSM2

Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL132C / CSM2

Click on Significant Values for Screen Details ID:SGTC_96|Compound:0929-0093|FD-Score:-3.00|P-value:5.14E-4 ID:SGTC_192|Compound:3455-0609|FD-Score:3.06|P-value:3.05E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.81|P-value:4.15E-8 ID:SGTC_314|Compound:1671-0008|FD-Score:3.29|P-value:1.15E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.35|P-value:9.01E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:2.95|P-value:4.84E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.94|P-value:4.89E-4 ID:SGTC_444|Compound:curcumin|FD-Score:2.91|P-value:5.56E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.53|P-value:5.51E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.25|P-value:1.36E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.12|P-value:2.40E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:2.77|P-value:9.36E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:3.13|P-value:2.33E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.19|P-value:1.81E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:2.76|P-value:9.76E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:2.91|P-value:5.64E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.73|P-value:1.56E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:4.24|P-value:1.08E-6 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.17|P-value:1.97E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-3.14|P-value:2.95E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.12|P-value:2.34E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.69|P-value:1.88E-5 ID:SGTC_1726|Compound:st035556|FD-Score:-2.94|P-value:6.57E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-3.17|P-value:2.60E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.14|P-value:2.21E-4 ID:SGTC_1886|Compound:st057356|FD-Score:2.99|P-value:4.14E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-2.91|P-value:7.17E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-3.07|P-value:3.85E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.23|P-value:1.48E-4 ID:SGTC_2292|Compound:7671323|FD-Score:5.17|P-value:4.02E-9 ID:SGTC_2714|Compound:st079313|FD-Score:-3.12|P-value:3.19E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.28|P-value:8.71E-7 ID:SGTC_3202|Compound:9113761|FD-Score:3.82|P-value:1.01E-5 ID:SGTC_3327|Compound:9140648|FD-Score:3.68|P-value:1.99E-5 ID:SGTC_3330|Compound:9141424|FD-Score:3.19|P-value:1.78E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.00|P-value:5.14E-4 ID:SGTC_192|Compound:3455-0609|FD-Score:3.06|P-value:3.05E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.81|P-value:4.15E-8 ID:SGTC_314|Compound:1671-0008|FD-Score:3.29|P-value:1.15E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.35|P-value:9.01E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:2.95|P-value:4.84E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:2.94|P-value:4.89E-4 ID:SGTC_444|Compound:curcumin|FD-Score:2.91|P-value:5.56E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.53|P-value:5.51E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.25|P-value:1.36E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.12|P-value:2.40E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:2.77|P-value:9.36E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:3.13|P-value:2.33E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.19|P-value:1.81E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:2.76|P-value:9.76E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:2.91|P-value:5.64E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:3.73|P-value:1.56E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:4.24|P-value:1.08E-6 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.17|P-value:1.97E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-3.14|P-value:2.95E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.12|P-value:2.34E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.69|P-value:1.88E-5 ID:SGTC_1726|Compound:st035556|FD-Score:-2.94|P-value:6.57E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-3.17|P-value:2.60E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.14|P-value:2.21E-4 ID:SGTC_1886|Compound:st057356|FD-Score:2.99|P-value:4.14E-4 ID:SGTC_1898|Compound:st060199|FD-Score:-2.91|P-value:7.17E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-3.07|P-value:3.85E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.23|P-value:1.48E-4 ID:SGTC_2292|Compound:7671323|FD-Score:5.17|P-value:4.02E-9 ID:SGTC_2714|Compound:st079313|FD-Score:-3.12|P-value:3.19E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.28|P-value:8.71E-7 ID:SGTC_3202|Compound:9113761|FD-Score:3.82|P-value:1.01E-5 ID:SGTC_3327|Compound:9140648|FD-Score:3.68|P-value:1.99E-5 ID:SGTC_3330|Compound:9141424|FD-Score:3.19|P-value:1.78E-4

Top fitness defect scores for YIL132C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2292 7671323 5.17 168.1 µM 4.02E-9 RNA processing & uracil transport 13 1.14E-7
2 SGTC_248 streptozotocin 4.81 5.2 mM 4.15E-8 DNA damage response 16 7.67E-7
3 SGTC_2789 5281112 4.28 71.4 µM 8.71E-7 25 9.19E-6
4 SGTC_1492 4158-0558 4.24 28.5 µM 1.08E-6 60S ribosome export 9 1.10E-5
5 SGTC_3202 9113761 3.82 49.5 µM 1.01E-5 51 6.79E-5
6 SGTC_1456 k015-0012 3.73 13.0 µM 1.56E-5 39 9.67E-5
7 SGTC_1725 st036284 3.69 20.6 µM 1.88E-5 37 1.13E-4
8 SGTC_3327 9140648 3.68 26.9 µM 1.99E-5 14 1.18E-4
9 SGTC_350 k064-0020 3.35 19.8 µM 9.01E-5 heme requiring 46 4.07E-4
10 SGTC_314 1671-0008 3.29 2.7 µM 1.15E-4 87 4.97E-4
11 SGTC_797 k781-0471 3.25 270.0 µM 1.36E-4 RPP1 & pyrimidine depletion 44 5.70E-4
12 SGTC_1936 st074407 3.23 74.3 µM 1.48E-4 DNA damage response 78 6.10E-4
13 SGTC_3330 9141424 3.19 71.4 µM 1.78E-4 amide catabolism 46 7.09E-4
14 SGTC_1128 3448-9350 3.19 235.0 µM 1.81E-4 54 7.20E-4
15 SGTC_1579 chlormadinone acetate 3.17 49.4 µM 1.97E-4 34 7.73E-4
16 SGTC_1764 st044502 3.14 93.5 µM 2.21E-4 60 8.46E-4
17 SGTC_992 3909-8734 3.13 162.0 µM 2.33E-4 49 8.83E-4
18 SGTC_1707 st032323 3.12 6.3 µM 2.34E-4 DNA damage response 102 8.89E-4
19 SGTC_845 0330-0138 3.12 4.5 µM 2.40E-4 48 9.07E-4
20 SGTC_192 3455-0609 3.06 60.9 µM 3.05E-4 51 0.00110

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 5.03E-13 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.123 7.68E-13 YBL068W PRS4 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication
0.122 1.15E-12 YNL281W HCH1 hom Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
0.121 2.41E-12 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.120 3.77E-12 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.117 1.13E-11 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.115 2.12E-11 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.105 1.21E-9 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.102 2.75E-9 YOR266W PNT1 hom Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
0.102 3.39E-9 YDL218W_p YDL218W_p hom Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin
0.100 6.84E-9 YML047W-A_d YML047W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.097 1.55E-8 YER142C MAG1 hom 3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress
0.096 2.96E-8 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.093 6.01E-8 YOL030W GAS5 hom 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall
0.092 8.64E-8 YBL092W RPL32 het Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog