YIL135C / VHS2

Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YIL135C / VHS2

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:4.37|P-value:4.97E-7 ID:SGTC_41|Compound:0478-0632|FD-Score:4.59|P-value:1.36E-7 ID:SGTC_145|Compound:3737-0113|FD-Score:-3.03|P-value:4.62E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:2.74|P-value:9.90E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.90|P-value:5.50E-4 ID:SGTC_180|Compound:1486-1293|FD-Score:2.95|P-value:4.51E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.08|P-value:2.65E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:4.02|P-value:3.20E-6 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.41|P-value:6.31E-5 ID:SGTC_486|Compound:niguldipine|FD-Score:2.81|P-value:7.60E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.26|P-value:1.78E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:3.04|P-value:3.10E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.44|P-value:5.57E-5 ID:SGTC_771|Compound:0250-0039|FD-Score:2.98|P-value:3.99E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:2.99|P-value:3.82E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:2.87|P-value:6.22E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:3.40|P-value:6.78E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.43|P-value:5.42E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.97|P-value:4.22E-6 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:6.01|P-value:9.26E-12 ID:SGTC_1696|Compound:st025591|FD-Score:3.03|P-value:3.24E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.25|P-value:1.30E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.18|P-value:1.73E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.10|P-value:2.42E-4 ID:SGTC_2008|Compound:4012253|FD-Score:2.97|P-value:4.09E-4 ID:SGTC_2012|Compound:4023501|FD-Score:2.99|P-value:3.82E-4 ID:SGTC_2040|Compound:5100729|FD-Score:3.75|P-value:1.26E-5 ID:SGTC_2158|Compound:5657123|FD-Score:2.98|P-value:3.96E-4 ID:SGTC_2256|Compound:7954252|FD-Score:-2.95|P-value:6.11E-4 ID:SGTC_2312|Compound:7706453|FD-Score:4.06|P-value:2.60E-6 ID:SGTC_2463|Compound:5473429|FD-Score:3.30|P-value:1.02E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:6.44|P-value:3.10E-13 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-2.89|P-value:7.72E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.08|P-value:2.64E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:2.95|P-value:4.39E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:6.38|P-value:5.03E-13 ID:SGTC_2651|Compound:1327-0036|FD-Score:10.20|P-value:3.05E-30 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.13|P-value:2.19E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.83|P-value:1.19E-14 ID:SGTC_3002|Compound:9075603|FD-Score:-3.48|P-value:6.88E-5 ID:SGTC_3009|Compound:9080819|FD-Score:3.36|P-value:7.79E-5 ID:SGTC_3132|Compound:9126706|FD-Score:3.14|P-value:2.05E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-4.31|P-value:1.08E-6 ID:SGTC_3181|Compound:9108277|FD-Score:-3.90|P-value:9.10E-6 ID:SGTC_39|Compound:0986-0283|FD-Score:4.37|P-value:4.97E-7 ID:SGTC_41|Compound:0478-0632|FD-Score:4.59|P-value:1.36E-7 ID:SGTC_145|Compound:3737-0113|FD-Score:-3.03|P-value:4.62E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:2.74|P-value:9.90E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:2.90|P-value:5.50E-4 ID:SGTC_180|Compound:1486-1293|FD-Score:2.95|P-value:4.51E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.08|P-value:2.65E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:4.02|P-value:3.20E-6 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.41|P-value:6.31E-5 ID:SGTC_486|Compound:niguldipine|FD-Score:2.81|P-value:7.60E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.26|P-value:1.78E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:3.04|P-value:3.10E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.44|P-value:5.57E-5 ID:SGTC_771|Compound:0250-0039|FD-Score:2.98|P-value:3.99E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:2.99|P-value:3.82E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:2.87|P-value:6.22E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:3.40|P-value:6.78E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.43|P-value:5.42E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.97|P-value:4.22E-6 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:6.01|P-value:9.26E-12 ID:SGTC_1696|Compound:st025591|FD-Score:3.03|P-value:3.24E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.25|P-value:1.30E-4 ID:SGTC_1755|Compound:st041960|FD-Score:3.18|P-value:1.73E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.10|P-value:2.42E-4 ID:SGTC_2008|Compound:4012253|FD-Score:2.97|P-value:4.09E-4 ID:SGTC_2012|Compound:4023501|FD-Score:2.99|P-value:3.82E-4 ID:SGTC_2040|Compound:5100729|FD-Score:3.75|P-value:1.26E-5 ID:SGTC_2158|Compound:5657123|FD-Score:2.98|P-value:3.96E-4 ID:SGTC_2256|Compound:7954252|FD-Score:-2.95|P-value:6.11E-4 ID:SGTC_2312|Compound:7706453|FD-Score:4.06|P-value:2.60E-6 ID:SGTC_2463|Compound:5473429|FD-Score:3.30|P-value:1.02E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:6.44|P-value:3.10E-13 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-2.89|P-value:7.72E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.08|P-value:2.64E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:2.95|P-value:4.39E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:6.38|P-value:5.03E-13 ID:SGTC_2651|Compound:1327-0036|FD-Score:10.20|P-value:3.05E-30 ID:SGTC_2685|Compound:mycophenolic acid|FD-Score:3.13|P-value:2.19E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.83|P-value:1.19E-14 ID:SGTC_3002|Compound:9075603|FD-Score:-3.48|P-value:6.88E-5 ID:SGTC_3009|Compound:9080819|FD-Score:3.36|P-value:7.79E-5 ID:SGTC_3132|Compound:9126706|FD-Score:3.14|P-value:2.05E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-4.31|P-value:1.08E-6 ID:SGTC_3181|Compound:9108277|FD-Score:-3.90|P-value:9.10E-6

Top fitness defect scores for YIL135C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2651 1327-0036 10.20 5.0 µM 3.05E-30 24 1.03E-24
2 SGTC_2688 kanamycin b 6.83 96.7 µM 1.19E-14 calcium & mitochondrial duress 112 4.29E-12
3 SGTC_2560 caryophyllene 6.44 100.0 µM 3.10E-13 2 5.99E-11
4 SGTC_2640 pentamidine 6.38 10.4 µM 5.03E-13 mitochondrial processes 4 8.86E-11
5 SGTC_1309 pyrazolanthrone 6.01 13.4 µM 9.26E-12 8 9.37E-10
6 SGTC_41 0478-0632 4.59 82.6 µM 1.36E-7 16 2.23E-6
7 SGTC_39 0986-0283 4.37 58.4 µM 4.97E-7 calcium & mitochondrial duress 27 6.35E-6
8 SGTC_2312 7706453 4.06 155.7 µM 2.60E-6 26 2.43E-5
9 SGTC_435 c-PAF 4.02 6.7 µM 3.20E-6 39 2.88E-5
10 SGTC_1251 0490-5699 3.97 265.0 µM 4.22E-6 32 3.60E-5
11 SGTC_2040 5100729 3.75 123.0 µM 1.26E-5 47 8.77E-5
12 SGTC_759 3486-0358 3.44 89.3 µM 5.57E-5 33 2.92E-4
13 SGTC_473 2-arachidonoylglycerol 3.41 10.0 µM 6.31E-5 30 3.23E-4
14 SGTC_882 0843-0131 3.40 351.0 µM 6.78E-5 28 3.43E-4
15 SGTC_3009 9080819 3.36 71.4 µM 7.79E-5 31 3.84E-4
16 SGTC_2463 5473429 3.30 200.0 µM 1.02E-4 ERG2 36 4.78E-4
17 SGTC_1698 5-fluorocytosine 3.25 1.1 µM 1.30E-4 exosome 22 5.81E-4
18 SGTC_1755 st041960 3.18 45.2 µM 1.73E-4 46 7.34E-4
19 SGTC_3132 9126706 3.14 49.5 µM 2.05E-4 57 8.41E-4
20 SGTC_2685 mycophenolic acid 3.13 18.9 µM 2.19E-4 RNA pol III & RNase P/MRP 27 8.88E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.373 5.27E-111 YIL107C PFK26 hom 6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A
0.273 3.18E-58 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.271 1.47E-57 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.260 7.21E-53 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.259 1.53E-52 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.248 4.28E-48 YIL069C RPS24B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication
0.233 1.43E-42 YIL057C RGI2 hom Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose
0.226 2.96E-40 YIR012W SQT1 het Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
0.213 1.05E-35 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.201 8.38E-32 YIL124W AYR1 hom NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones
0.199 1.97E-31 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.191 4.74E-29 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.186 1.86E-27 YLL038C ENT4 hom Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
0.182 1.60E-26 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.181 3.41E-26 YIL022W TIM44 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)