YIL143C / SSL2

Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3

Zygosity: Heterozygous strain
fixedexpanded
Profile for YIL143C / SSL2

Click on Significant Values for Screen Details ID:SGTC_124|Compound:0136-0217|FD-Score:2.88|P-value:8.66E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:3.09|P-value:3.89E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.04|P-value:4.68E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:45.60|P-value:0 ID:SGTC_272|Compound:doxorubicin|FD-Score:52.60|P-value:0 ID:SGTC_291|Compound:4130-1278|FD-Score:3.25|P-value:1.99E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.48|P-value:7.49E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:2.88|P-value:8.71E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.20|P-value:2.42E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-5.93|P-value:5.03E-11 ID:SGTC_919|Compound:tnp00227|FD-Score:3.33|P-value:1.44E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.93|P-value:9.33E-6 ID:SGTC_948|Compound:3448-0295|FD-Score:3.99|P-value:6.84E-6 ID:SGTC_1085|Compound:doxorubicin|FD-Score:23.10|P-value:1.67E-139 ID:SGTC_1105|Compound:epirubicin|FD-Score:10.60|P-value:3.28E-31 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.04|P-value:4.70E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.51|P-value:6.73E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:4.59|P-value:2.97E-7 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.11|P-value:3.77E-6 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.94|P-value:9.11E-6 ID:SGTC_1912|Compound:st060209|FD-Score:-3.39|P-value:1.09E-4 ID:SGTC_2028|Compound:5153890|FD-Score:5.62|P-value:4.55E-10 ID:SGTC_2029|Compound:5175081|FD-Score:2.98|P-value:5.98E-4 ID:SGTC_2035|Compound:5154463|FD-Score:-4.26|P-value:1.69E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-3.40|P-value:1.07E-4 ID:SGTC_2043|Compound:5101342|FD-Score:-4.84|P-value:6.68E-8 ID:SGTC_2068|Compound:5226020|FD-Score:4.21|P-value:2.29E-6 ID:SGTC_2089|Compound:5281248|FD-Score:3.94|P-value:9.06E-6 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.93|P-value:3.86E-8 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.28|P-value:1.76E-4 ID:SGTC_2290|Compound:7904846|FD-Score:-3.26|P-value:1.87E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.63|P-value:2.37E-7 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.47|P-value:7.94E-5 ID:SGTC_2412|Compound:st077135|FD-Score:4.30|P-value:1.39E-6 ID:SGTC_2439|Compound:5492030|FD-Score:3.10|P-value:3.65E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-2.87|P-value:8.95E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:6.18|P-value:8.16E-12 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.07|P-value:4.67E-6 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.05|P-value:4.43E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-4.92|P-value:4.24E-8 ID:SGTC_2662|Compound:daunorubicin|FD-Score:30.30|P-value:9.77E-239 ID:SGTC_2746|Compound:daunorubicin|FD-Score:6.71|P-value:1.34E-13 ID:SGTC_2747|Compound:doxorubicin|FD-Score:11.30|P-value:5.07E-35 ID:SGTC_2796|Compound:7949634|FD-Score:-4.15|P-value:3.10E-6 ID:SGTC_2798|Compound:7813637|FD-Score:-4.89|P-value:4.99E-8 ID:SGTC_2803|Compound:7707263|FD-Score:4.26|P-value:1.77E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-6.27|P-value:4.37E-12 ID:SGTC_3054|Compound:9094397|FD-Score:2.89|P-value:8.24E-4 ID:SGTC_3127|Compound:9126946|FD-Score:3.20|P-value:2.47E-4 ID:SGTC_3144|Compound:9087057|FD-Score:4.16|P-value:2.95E-6 ID:SGTC_3296|Compound:9122258|FD-Score:3.18|P-value:2.69E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:2.88|P-value:8.66E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:3.09|P-value:3.89E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.04|P-value:4.68E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:45.60|P-value:0 ID:SGTC_272|Compound:doxorubicin|FD-Score:52.60|P-value:0 ID:SGTC_291|Compound:4130-1278|FD-Score:3.25|P-value:1.99E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.48|P-value:7.49E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:2.88|P-value:8.71E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:3.20|P-value:2.42E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-5.93|P-value:5.03E-11 ID:SGTC_919|Compound:tnp00227|FD-Score:3.33|P-value:1.44E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.93|P-value:9.33E-6 ID:SGTC_948|Compound:3448-0295|FD-Score:3.99|P-value:6.84E-6 ID:SGTC_1085|Compound:doxorubicin|FD-Score:23.10|P-value:1.67E-139 ID:SGTC_1105|Compound:epirubicin|FD-Score:10.60|P-value:3.28E-31 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.04|P-value:4.70E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.51|P-value:6.73E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:4.59|P-value:2.97E-7 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.11|P-value:3.77E-6 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.94|P-value:9.11E-6 ID:SGTC_1912|Compound:st060209|FD-Score:-3.39|P-value:1.09E-4 ID:SGTC_2028|Compound:5153890|FD-Score:5.62|P-value:4.55E-10 ID:SGTC_2029|Compound:5175081|FD-Score:2.98|P-value:5.98E-4 ID:SGTC_2035|Compound:5154463|FD-Score:-4.26|P-value:1.69E-6 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-3.40|P-value:1.07E-4 ID:SGTC_2043|Compound:5101342|FD-Score:-4.84|P-value:6.68E-8 ID:SGTC_2068|Compound:5226020|FD-Score:4.21|P-value:2.29E-6 ID:SGTC_2089|Compound:5281248|FD-Score:3.94|P-value:9.06E-6 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:4.93|P-value:3.86E-8 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.28|P-value:1.76E-4 ID:SGTC_2290|Compound:7904846|FD-Score:-3.26|P-value:1.87E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.63|P-value:2.37E-7 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.47|P-value:7.94E-5 ID:SGTC_2412|Compound:st077135|FD-Score:4.30|P-value:1.39E-6 ID:SGTC_2439|Compound:5492030|FD-Score:3.10|P-value:3.65E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-2.87|P-value:8.95E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:6.18|P-value:8.16E-12 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.07|P-value:4.67E-6 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.05|P-value:4.43E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-4.92|P-value:4.24E-8 ID:SGTC_2662|Compound:daunorubicin|FD-Score:30.30|P-value:9.77E-239 ID:SGTC_2746|Compound:daunorubicin|FD-Score:6.71|P-value:1.34E-13 ID:SGTC_2747|Compound:doxorubicin|FD-Score:11.30|P-value:5.07E-35 ID:SGTC_2796|Compound:7949634|FD-Score:-4.15|P-value:3.10E-6 ID:SGTC_2798|Compound:7813637|FD-Score:-4.89|P-value:4.99E-8 ID:SGTC_2803|Compound:7707263|FD-Score:4.26|P-value:1.77E-6 ID:SGTC_2965|Compound:9082772|FD-Score:-6.27|P-value:4.37E-12 ID:SGTC_3054|Compound:9094397|FD-Score:2.89|P-value:8.24E-4 ID:SGTC_3127|Compound:9126946|FD-Score:3.20|P-value:2.47E-4 ID:SGTC_3144|Compound:9087057|FD-Score:4.16|P-value:2.95E-6 ID:SGTC_3296|Compound:9122258|FD-Score:3.18|P-value:2.69E-4

Top fitness defect scores for YIL143C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_272 doxorubicin 52.60 8.1 µM 0 anthracycline transcription coupled DNA repair 1 0 42.30
2 SGTC_257 daunorubicin 45.60 18.5 µM 0 anthracycline transcription coupled DNA repair 1 0 31.40
3 SGTC_2662 daunorubicin 30.30 22.4 µM 9.77E-239 anthracycline transcription coupled DNA repair 1 1.33E-201 20.40
4 SGTC_1085 doxorubicin 23.10 7.7 µM 1.67E-139 anthracycline transcription coupled DNA repair 1 6.02E-118 16.80
5 SGTC_2747 doxorubicin 11.30 7.6 µM 5.07E-35 anthracycline transcription coupled DNA repair 1 7.80E-30 3.19
6 SGTC_1105 epirubicin 10.60 18.1 µM 3.28E-31 anthracycline transcription coupled DNA repair 1 1.28E-26 4.17
7 SGTC_2746 daunorubicin 6.71 9.9 µM 1.34E-13 anthracycline transcription coupled DNA repair 1 9.60E-12 1.82
8 SGTC_2507 purpurin 6.18 79.7 µM 8.16E-12 copper-dependent oxidative stress 4 3.11E-10 0.14
9 SGTC_2028 5153890 5.62 36.4 µM 4.55E-10 cell wall signaling 3 9.35E-9 0.03
10 SGTC_2209 5-(5-methyl-2-thenylidene)-Barbituric acid 4.93 122.8 µM 3.86E-8 3 4.02E-7 0.79
11 SGTC_2408 sulbentine 4.63 18.4 µM 2.37E-7 25 1.87E-6 0.06
12 SGTC_1313 1220-0047 4.59 237.0 µM 2.97E-7 4 2.27E-6 0.08
13 SGTC_2412 st077135 4.30 33.4 µM 1.39E-6 5 8.39E-6 0.08
14 SGTC_2803 7707263 4.26 71.4 µM 1.77E-6 2 1.03E-5 0.50
15 SGTC_2068 5226020 4.21 147.0 µM 2.29E-6 4 1.28E-5 0.15
16 SGTC_3144 9087057 4.16 49.5 µM 2.95E-6 3 1.59E-5 0.14
17 SGTC_1317 1273-0028 4.11 910.0 nM 3.77E-6 azole & statin 11 1.96E-5 0.10
18 SGTC_2510 benzyl isothiocyanate 4.07 8.3 µM 4.67E-6 24 2.35E-5 0.18
19 SGTC_948 3448-0295 3.99 75.3 µM 6.84E-6 9 3.25E-5 0.06
20 SGTC_2089 5281248 3.94 129.7 µM 9.06E-6 superoxide 11 4.13E-5 0.29

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 1.63E-30 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.175 1.33E-24 YMR146C TIF34 het eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
0.173 5.55E-24 YPL161C BEM4 hom Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
0.164 1.25E-21 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.146 1.75E-17 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.146 1.91E-17 YGR098C ESP1 het Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.128 8.81E-14 YNL202W SPS19 hom Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
0.127 1.35E-13 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.126 2.89E-13 YIR018C-A_p YIR018C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.124 5.86E-13 YLR428C_d YLR428C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1
0.123 1.03E-12 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.119 5.28E-12 YHR185C PFS1 hom Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation
0.114 4.33E-11 YBR069C TAT1 hom Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
0.113 6.14E-11 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.111 1.30E-10 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway