YIR002C / MPH1

3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases

Zygosity: Homozygous strain
fixedexpanded
Profile for YIR002C / MPH1

Click on Significant Values for Screen Details ID:SGTC_113|Compound:0099-0221|FD-Score:3.38|P-value:5.98E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.92|P-value:1.23E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.93|P-value:1.32E-6 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.62|P-value:3.54E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.12|P-value:4.12E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:6.62|P-value:1.50E-10 ID:SGTC_693|Compound:4161-2696|FD-Score:-3.50|P-value:5.39E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:4.02|P-value:6.02E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:3.48|P-value:4.29E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.84|P-value:1.21E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:-3.62|P-value:3.59E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:-4.80|P-value:3.37E-6 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.45|P-value:4.85E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.39|P-value:5.89E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.65|P-value:2.44E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.27|P-value:8.65E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.79|P-value:2.48E-6 ID:SGTC_1422|Compound:4031-0024|FD-Score:3.96|P-value:7.69E-5 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.20|P-value:3.06E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:3.63|P-value:2.58E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:5.23|P-value:3.13E-7 ID:SGTC_1591|Compound:genipin|FD-Score:-3.41|P-value:7.07E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.94|P-value:1.15E-4 ID:SGTC_1781|Compound:st038725|FD-Score:3.45|P-value:4.74E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.68|P-value:2.17E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.26|P-value:8.89E-4 ID:SGTC_1933|Compound:st074711|FD-Score:-3.42|P-value:7.00E-4 ID:SGTC_1936|Compound:st074407|FD-Score:8.73|P-value:5.23E-17 ID:SGTC_2329|Compound:6138490|FD-Score:4.25|P-value:2.45E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.75|P-value:2.94E-6 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:4.26|P-value:2.34E-5 ID:SGTC_2557|Compound:derrustone|FD-Score:3.85|P-value:1.18E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.10|P-value:4.57E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:5.29|P-value:2.26E-7 ID:SGTC_2677|Compound:mitomycin C|FD-Score:4.23|P-value:2.67E-5 ID:SGTC_2906|Compound:7926292|FD-Score:3.27|P-value:8.83E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.74|P-value:1.77E-4 ID:SGTC_3027|Compound:9089541|FD-Score:5.29|P-value:2.34E-7 ID:SGTC_3058|Compound:9093861|FD-Score:4.54|P-value:7.35E-6 ID:SGTC_3101|Compound:9120531|FD-Score:-5.16|P-value:6.29E-7 ID:SGTC_3189|Compound:9110682|FD-Score:4.64|P-value:4.80E-6 ID:SGTC_3195|Compound:9113082|FD-Score:3.26|P-value:8.91E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.30|P-value:2.05E-5 ID:SGTC_3238|Compound:9133470|FD-Score:3.35|P-value:6.71E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.95|P-value:1.08E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.28|P-value:8.43E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:3.38|P-value:5.98E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.92|P-value:1.23E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.93|P-value:1.32E-6 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.62|P-value:3.54E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.12|P-value:4.12E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:6.62|P-value:1.50E-10 ID:SGTC_693|Compound:4161-2696|FD-Score:-3.50|P-value:5.39E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:4.02|P-value:6.02E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:3.48|P-value:4.29E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.84|P-value:1.21E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:-3.62|P-value:3.59E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:-4.80|P-value:3.37E-6 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.45|P-value:4.85E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.39|P-value:5.89E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.65|P-value:2.44E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.27|P-value:8.65E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:4.79|P-value:2.48E-6 ID:SGTC_1422|Compound:4031-0024|FD-Score:3.96|P-value:7.69E-5 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.20|P-value:3.06E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:3.63|P-value:2.58E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:5.23|P-value:3.13E-7 ID:SGTC_1591|Compound:genipin|FD-Score:-3.41|P-value:7.07E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:-3.94|P-value:1.15E-4 ID:SGTC_1781|Compound:st038725|FD-Score:3.45|P-value:4.74E-4 ID:SGTC_1820|Compound:st053765|FD-Score:3.68|P-value:2.17E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.26|P-value:8.89E-4 ID:SGTC_1933|Compound:st074711|FD-Score:-3.42|P-value:7.00E-4 ID:SGTC_1936|Compound:st074407|FD-Score:8.73|P-value:5.23E-17 ID:SGTC_2329|Compound:6138490|FD-Score:4.25|P-value:2.45E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.75|P-value:2.94E-6 ID:SGTC_2554|Compound:13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one|FD-Score:4.26|P-value:2.34E-5 ID:SGTC_2557|Compound:derrustone|FD-Score:3.85|P-value:1.18E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.10|P-value:4.57E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:5.29|P-value:2.26E-7 ID:SGTC_2677|Compound:mitomycin C|FD-Score:4.23|P-value:2.67E-5 ID:SGTC_2906|Compound:7926292|FD-Score:3.27|P-value:8.83E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.74|P-value:1.77E-4 ID:SGTC_3027|Compound:9089541|FD-Score:5.29|P-value:2.34E-7 ID:SGTC_3058|Compound:9093861|FD-Score:4.54|P-value:7.35E-6 ID:SGTC_3101|Compound:9120531|FD-Score:-5.16|P-value:6.29E-7 ID:SGTC_3189|Compound:9110682|FD-Score:4.64|P-value:4.80E-6 ID:SGTC_3195|Compound:9113082|FD-Score:3.26|P-value:8.91E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.30|P-value:2.05E-5 ID:SGTC_3238|Compound:9133470|FD-Score:3.35|P-value:6.71E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.95|P-value:1.08E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.28|P-value:8.43E-4

Top fitness defect scores for YIR002C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1936 st074407 8.73 74.3 µM 5.23E-17 DNA damage response 2 1.23E-18
2 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 6.62 44.4 nM 1.50E-10 DNA damage response 17 1.81E-11
3 SGTC_2583 asiatic acid 5.29 100.0 µM 2.26E-7 18 5.98E-8
4 SGTC_3027 9089541 5.29 49.5 µM 2.34E-7 fatty acid desaturase (OLE1) 4 6.22E-8
5 SGTC_1584 6-fluoro-dl-tryptophan 5.23 90.0 µM 3.13E-7 mitochondrial processes 4 8.60E-8
6 SGTC_184 1494-0393 4.93 24.7 µM 1.32E-6 34 4.22E-7
7 SGTC_1351 1496-0003 4.79 46.5 µM 2.48E-6 9 8.47E-7
8 SGTC_2406 ethylparaben 4.75 405.7 µM 2.94E-6 18 1.02E-6
9 SGTC_3189 9110682 4.64 49.5 µM 4.80E-6 22 1.75E-6
10 SGTC_3058 9093861 4.54 49.5 µM 7.35E-6 9 2.81E-6
11 SGTC_3210 9128157 4.30 49.5 µM 2.05E-5 39 8.72E-6
12 SGTC_2554 13-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one 4.26 66.7 µM 2.34E-5 14 1.01E-5
13 SGTC_2329 6138490 4.25 200.0 µM 2.45E-5 22 1.06E-5
14 SGTC_2677 mitomycin C 4.23 100.0 µM 2.67E-5 DNA damage response 13 1.16E-5
15 SGTC_1425 4048-4433 4.20 7.3 µM 3.06E-5 copper-dependent oxidative stress 33 1.35E-5
16 SGTC_248 streptozotocin 4.12 5.2 mM 4.12E-5 DNA damage response 23 1.88E-5
17 SGTC_2560 caryophyllene 4.10 100.0 µM 4.57E-5 15 2.11E-5
18 SGTC_864 0469-0573 4.02 172.0 µM 6.02E-5 17 2.85E-5
19 SGTC_1422 4031-0024 3.96 15.5 µM 7.69E-5 iron homeostasis 17 3.74E-5
20 SGTC_2557 derrustone 3.85 83.8 µM 1.18E-4 37 5.96E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.266 3.60E-55 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.239 1.02E-44 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.233 2.28E-42 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.221 3.17E-38 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.185 2.53E-27 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.184 5.16E-27 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.162 5.11E-21 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.161 5.65E-21 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.152 6.95E-19 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.135 4.76E-15 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.134 8.18E-15 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.130 4.78E-14 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.127 2.01E-13 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.121 1.83E-12 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.118 5.91E-12 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability