YIR005W / IST3

Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing

Zygosity: Homozygous strain
fixedexpanded
Profile for YIR005W / IST3

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:-5.49|P-value:3.16E-8 ID:SGTC_72|Compound:0744-0168|FD-Score:3.43|P-value:2.37E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.35|P-value:5.15E-6 ID:SGTC_370|Compound:0180-0345|FD-Score:-3.22|P-value:8.51E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:4.11|P-value:1.51E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:-3.20|P-value:8.88E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:3.31|P-value:3.62E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:-3.33|P-value:5.68E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.66|P-value:9.75E-5 ID:SGTC_895|Compound:0964-0006|FD-Score:3.38|P-value:2.90E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.70|P-value:9.40E-7 ID:SGTC_995|Compound:3931-2021|FD-Score:3.36|P-value:3.12E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-4.48|P-value:5.47E-6 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.54|P-value:2.72E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:6.65|P-value:9.50E-12 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.79|P-value:1.03E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.63|P-value:1.12E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.15|P-value:6.49E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.76|P-value:6.60E-5 ID:SGTC_1184|Compound:1269-1718|FD-Score:4.26|P-value:7.73E-6 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.05|P-value:9.28E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.22|P-value:8.39E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:4.29|P-value:6.79E-6 ID:SGTC_1705|Compound:st027868|FD-Score:3.21|P-value:5.34E-4 ID:SGTC_1969|Compound:st069479|FD-Score:3.42|P-value:2.42E-4 ID:SGTC_2027|Compound:5150920|FD-Score:4.77|P-value:6.62E-7 ID:SGTC_2050|Compound:5263056|FD-Score:5.22|P-value:6.44E-8 ID:SGTC_2051|Compound:5263410|FD-Score:-4.55|P-value:3.90E-6 ID:SGTC_2060|Compound:5238662|FD-Score:3.25|P-value:4.54E-4 ID:SGTC_2101|Compound:5377231|FD-Score:3.34|P-value:3.30E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.96|P-value:2.82E-5 ID:SGTC_2255|Compound:7941087|FD-Score:3.20|P-value:5.54E-4 ID:SGTC_2259|Compound:7964009|FD-Score:5.43|P-value:1.94E-8 ID:SGTC_2289|Compound:7946404|FD-Score:3.27|P-value:4.24E-4 ID:SGTC_2290|Compound:7904846|FD-Score:3.05|P-value:9.13E-4 ID:SGTC_2431|Compound:5490079|FD-Score:6.45|P-value:3.68E-11 ID:SGTC_2434|Compound:5670830|FD-Score:-3.33|P-value:5.66E-4 ID:SGTC_2460|Compound:5552655|FD-Score:4.23|P-value:8.59E-6 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.71|P-value:9.24E-7 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.44|P-value:2.28E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:4.95|P-value:2.71E-7 ID:SGTC_2646|Compound:retinol|FD-Score:3.34|P-value:3.25E-4 ID:SGTC_2705|Compound:st078069|FD-Score:3.54|P-value:1.58E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.42|P-value:4.20E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-4.87|P-value:8.41E-7 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.56|P-value:2.49E-4 ID:SGTC_2780|Compound:5215298|FD-Score:3.10|P-value:7.76E-4 ID:SGTC_2785|Compound:7756463|FD-Score:4.01|P-value:2.29E-5 ID:SGTC_2857|Compound:9024691|FD-Score:-3.69|P-value:1.54E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.73|P-value:1.30E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.69|P-value:1.55E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-4.10|P-value:2.95E-5 ID:SGTC_3216|Compound:9129716|FD-Score:3.21|P-value:5.27E-4 ID:SGTC_3336|Compound:9143619|FD-Score:4.30|P-value:6.28E-6 ID:SGTC_3351|Compound:9150312|FD-Score:3.47|P-value:2.06E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:-5.49|P-value:3.16E-8 ID:SGTC_72|Compound:0744-0168|FD-Score:3.43|P-value:2.37E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.35|P-value:5.15E-6 ID:SGTC_370|Compound:0180-0345|FD-Score:-3.22|P-value:8.51E-4 ID:SGTC_589|Compound:1227-0070|FD-Score:4.11|P-value:1.51E-5 ID:SGTC_626|Compound:0214-0009|FD-Score:-3.20|P-value:8.88E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:3.31|P-value:3.62E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:-3.33|P-value:5.68E-4 ID:SGTC_866|Compound:0495-0101|FD-Score:3.66|P-value:9.75E-5 ID:SGTC_895|Compound:0964-0006|FD-Score:3.38|P-value:2.90E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.70|P-value:9.40E-7 ID:SGTC_995|Compound:3931-2021|FD-Score:3.36|P-value:3.12E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-4.48|P-value:5.47E-6 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.54|P-value:2.72E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:6.65|P-value:9.50E-12 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.79|P-value:1.03E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.63|P-value:1.12E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:3.15|P-value:6.49E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.76|P-value:6.60E-5 ID:SGTC_1184|Compound:1269-1718|FD-Score:4.26|P-value:7.73E-6 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.05|P-value:9.28E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.22|P-value:8.39E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:4.29|P-value:6.79E-6 ID:SGTC_1705|Compound:st027868|FD-Score:3.21|P-value:5.34E-4 ID:SGTC_1969|Compound:st069479|FD-Score:3.42|P-value:2.42E-4 ID:SGTC_2027|Compound:5150920|FD-Score:4.77|P-value:6.62E-7 ID:SGTC_2050|Compound:5263056|FD-Score:5.22|P-value:6.44E-8 ID:SGTC_2051|Compound:5263410|FD-Score:-4.55|P-value:3.90E-6 ID:SGTC_2060|Compound:5238662|FD-Score:3.25|P-value:4.54E-4 ID:SGTC_2101|Compound:5377231|FD-Score:3.34|P-value:3.30E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.96|P-value:2.82E-5 ID:SGTC_2255|Compound:7941087|FD-Score:3.20|P-value:5.54E-4 ID:SGTC_2259|Compound:7964009|FD-Score:5.43|P-value:1.94E-8 ID:SGTC_2289|Compound:7946404|FD-Score:3.27|P-value:4.24E-4 ID:SGTC_2290|Compound:7904846|FD-Score:3.05|P-value:9.13E-4 ID:SGTC_2431|Compound:5490079|FD-Score:6.45|P-value:3.68E-11 ID:SGTC_2434|Compound:5670830|FD-Score:-3.33|P-value:5.66E-4 ID:SGTC_2460|Compound:5552655|FD-Score:4.23|P-value:8.59E-6 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.71|P-value:9.24E-7 ID:SGTC_2555|Compound:cholic acid|FD-Score:3.44|P-value:2.28E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:4.95|P-value:2.71E-7 ID:SGTC_2646|Compound:retinol|FD-Score:3.34|P-value:3.25E-4 ID:SGTC_2705|Compound:st078069|FD-Score:3.54|P-value:1.58E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:-3.42|P-value:4.20E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-4.87|P-value:8.41E-7 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.56|P-value:2.49E-4 ID:SGTC_2780|Compound:5215298|FD-Score:3.10|P-value:7.76E-4 ID:SGTC_2785|Compound:7756463|FD-Score:4.01|P-value:2.29E-5 ID:SGTC_2857|Compound:9024691|FD-Score:-3.69|P-value:1.54E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.73|P-value:1.30E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.69|P-value:1.55E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-4.10|P-value:2.95E-5 ID:SGTC_3216|Compound:9129716|FD-Score:3.21|P-value:5.27E-4 ID:SGTC_3336|Compound:9143619|FD-Score:4.30|P-value:6.28E-6 ID:SGTC_3351|Compound:9150312|FD-Score:3.47|P-value:2.06E-4

Top fitness defect scores for YIR005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1119 parthenolide 6.65 318.5 nM 9.50E-12 TSC3-RPN4 6 1.42E-11
2 SGTC_2431 5490079 6.45 200.0 µM 3.68E-11 5 5.45E-11
3 SGTC_2259 7964009 5.43 200.0 µM 1.94E-8 superoxide 15 2.75E-8
4 SGTC_2050 5263056 5.22 171.0 µM 6.44E-8 fatty acid desaturase (OLE1) 22 9.02E-8
5 SGTC_2633 5,7-dihydroxyisoflavone 4.95 100.0 µM 2.71E-7 17 3.74E-7
6 SGTC_2027 5150920 4.77 200.0 µM 6.62E-7 4 9.06E-7
7 SGTC_2502 avocadyne acetate 4.71 18.6 µM 9.24E-7 15 1.26E-6
8 SGTC_994 3910-0338 4.70 138.0 µM 9.40E-7 calcium & mitochondrial duress 25 1.28E-6
9 SGTC_118 0083-0097 4.35 135.3 µM 5.15E-6 translation 97 6.90E-6
10 SGTC_3336 9143619 4.30 71.4 µM 6.28E-6 13 8.39E-6
11 SGTC_1305 1181-0477 4.29 144.0 µM 6.79E-6 9 9.06E-6
12 SGTC_1184 1269-1718 4.26 139.0 µM 7.73E-6 21 1.03E-5
13 SGTC_2460 5552655 4.23 174.2 µM 8.59E-6 amide catabolism 25 1.14E-5
14 SGTC_589 1227-0070 4.11 34.1 µM 1.51E-5 26 2.00E-5
15 SGTC_2785 7756463 4.01 72.7 µM 2.29E-5 28 3.01E-5
16 SGTC_2249 haloperidol 3.96 116.4 µM 2.82E-5 ergosterol depletion effects on membrane 111 3.70E-5
17 SGTC_1177 0983-0204 3.76 23.6 µM 6.60E-5 19 8.57E-5
18 SGTC_866 0495-0101 3.66 262.0 µM 9.75E-5 60S ribosome export 27 1.26E-4
19 SGTC_1127 asiatic acid 3.63 681.4 nM 1.12E-4 33 1.44E-4
20 SGTC_2705 st078069 3.54 42.8 µM 1.58E-4 12 2.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.108 4.01E-10 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.106 6.59E-10 YBR082C UBC4 hom Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.104 1.60E-9 YAL033W POP5 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.103 1.94E-9 YOR080W DIA2 hom Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.100 5.67E-9 YML057C-A_d YML057C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
0.095 3.01E-8 YKR076W ECM4 hom Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.095 3.53E-8 YGL158W RCK1 hom Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication
0.088 3.62E-7 YJR056C YJR056C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.087 4.92E-7 YLR037C PAU23 hom Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
0.086 6.17E-7 YNL305C BXI1 hom Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
0.085 8.28E-7 YDR253C MET32 hom Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication
0.084 1.17E-6 YDR248C_p YDR248C_p hom Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
0.077 7.12E-6 YCR019W MAK32 hom Protein necessary for structural stability of L-A double-stranded RNA-containing particles
0.076 1.00E-5 YBR116C_d YBR116C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2
0.075 1.39E-5 YLL051C FRE6 hom Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels