YJL013C / MAD3

Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL013C / MAD3

Click on Significant Values for Screen Details ID:SGTC_514|Compound:4466-0038|FD-Score:3.99|P-value:8.34E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:3.18|P-value:2.98E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:-2.94|P-value:7.93E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.40|P-value:1.33E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.10|P-value:4.11E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.28|P-value:1.97E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.94|P-value:1.08E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:-3.29|P-value:2.08E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.32|P-value:1.72E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:2.96|P-value:7.01E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.72|P-value:3.33E-5 ID:SGTC_1670|Compound:st016595|FD-Score:-3.74|P-value:3.01E-5 ID:SGTC_1678|Compound:st019265|FD-Score:4.32|P-value:1.58E-6 ID:SGTC_1757|Compound:st041620|FD-Score:3.71|P-value:3.18E-5 ID:SGTC_1845|Compound:st056753|FD-Score:3.08|P-value:4.38E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.02|P-value:5.55E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.05|P-value:5.30E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.30|P-value:1.82E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.07|P-value:4.60E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-2.89|P-value:9.70E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.66|P-value:4.28E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.09|P-value:4.56E-4 ID:SGTC_2440|Compound:5628896|FD-Score:-4.52|P-value:6.05E-7 ID:SGTC_2446|Compound:5162541|FD-Score:-3.40|P-value:1.31E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.69|P-value:4.44E-10 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:-3.11|P-value:4.29E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-3.56|P-value:6.71E-5 ID:SGTC_2715|Compound:st079500|FD-Score:-3.32|P-value:1.83E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.13|P-value:3.66E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.16|P-value:3.25E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.60|P-value:5.11E-5 ID:SGTC_2895|Compound:9054447|FD-Score:-3.19|P-value:3.04E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.25|P-value:2.47E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.35|P-value:1.61E-4 ID:SGTC_3083|Compound:9117465|FD-Score:4.00|P-value:7.85E-6 ID:SGTC_3106|Compound:9121286|FD-Score:2.93|P-value:7.78E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.56|P-value:6.66E-5 ID:SGTC_3286|Compound:9117135|FD-Score:2.90|P-value:8.68E-4 ID:SGTC_3288|Compound:9119690|FD-Score:-2.92|P-value:8.68E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.51|P-value:7.67E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:3.99|P-value:8.34E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:3.18|P-value:2.98E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:-2.94|P-value:7.93E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.40|P-value:1.33E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.10|P-value:4.11E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.28|P-value:1.97E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.94|P-value:1.08E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:-3.29|P-value:2.08E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.32|P-value:1.72E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:2.96|P-value:7.01E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.72|P-value:3.33E-5 ID:SGTC_1670|Compound:st016595|FD-Score:-3.74|P-value:3.01E-5 ID:SGTC_1678|Compound:st019265|FD-Score:4.32|P-value:1.58E-6 ID:SGTC_1757|Compound:st041620|FD-Score:3.71|P-value:3.18E-5 ID:SGTC_1845|Compound:st056753|FD-Score:3.08|P-value:4.38E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.02|P-value:5.55E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.05|P-value:5.30E-4 ID:SGTC_2246|Compound:7293507|FD-Score:3.30|P-value:1.82E-4 ID:SGTC_2369|Compound:9071472|FD-Score:3.07|P-value:4.60E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-2.89|P-value:9.70E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.66|P-value:4.28E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.09|P-value:4.56E-4 ID:SGTC_2440|Compound:5628896|FD-Score:-4.52|P-value:6.05E-7 ID:SGTC_2446|Compound:5162541|FD-Score:-3.40|P-value:1.31E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.69|P-value:4.44E-10 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:-3.11|P-value:4.29E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-3.56|P-value:6.71E-5 ID:SGTC_2715|Compound:st079500|FD-Score:-3.32|P-value:1.83E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:3.13|P-value:3.66E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.16|P-value:3.25E-4 ID:SGTC_2789|Compound:5281112|FD-Score:3.60|P-value:5.11E-5 ID:SGTC_2895|Compound:9054447|FD-Score:-3.19|P-value:3.04E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.25|P-value:2.47E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.35|P-value:1.61E-4 ID:SGTC_3083|Compound:9117465|FD-Score:4.00|P-value:7.85E-6 ID:SGTC_3106|Compound:9121286|FD-Score:2.93|P-value:7.78E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.56|P-value:6.66E-5 ID:SGTC_3286|Compound:9117135|FD-Score:2.90|P-value:8.68E-4 ID:SGTC_3288|Compound:9119690|FD-Score:-2.92|P-value:8.68E-4 ID:SGTC_3298|Compound:9116858|FD-Score:3.51|P-value:7.67E-5

Top fitness defect scores for YJL013C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2651 1327-0036 5.69 5.0 µM 4.44E-10 82 6.53E-9
2 SGTC_1678 st019265 4.32 80.2 µM 1.58E-6 60S ribosome export 14 7.77E-6
3 SGTC_3083 9117465 4.00 49.5 µM 7.85E-6 23 3.12E-5
4 SGTC_514 4466-0038 3.99 2.6 nM 8.34E-6 cell wall 152 3.29E-5
5 SGTC_1450 4112-4260 3.94 37.6 µM 1.08E-5 calcium & mitochondrial duress 28 4.12E-5
6 SGTC_1757 st041620 3.71 31.8 µM 3.18E-5 36 1.05E-4
7 SGTC_2789 5281112 3.60 71.4 µM 5.11E-5 51 1.59E-4
8 SGTC_3298 9116858 3.51 71.4 µM 7.67E-5 20 2.26E-4
9 SGTC_2246 7293507 3.30 91.6 µM 1.82E-4 52 4.77E-4
10 SGTC_873 0591-4978 3.28 488.0 µM 1.97E-4 34 5.12E-4
11 SGTC_545 4218-0007 3.18 221.0 µM 2.98E-4 56 7.34E-4
12 SGTC_2784 7567673 3.16 72.7 µM 3.25E-4 iron homeostasis 65 7.92E-4
13 SGTC_2772 vinpocetine 3.13 57.1 µM 3.66E-4 56 8.78E-4
14 SGTC_779 1360-0071 3.10 233.0 µM 4.11E-4 fatty acid desaturase (OLE1) 71 9.71E-4
15 SGTC_1845 st056753 3.08 3.0 µM 4.38E-4 TSC3-RPN4 71 0.00103
16 SGTC_2369 9071472 3.07 200.0 µM 4.60E-4 49 0.00107
17 SGTC_1851 st056236 3.02 18.9 µM 5.55E-4 52 0.00126
18 SGTC_1464 k208-0027 2.96 110.0 µM 7.01E-4 60 0.00154
19 SGTC_3106 9121286 2.93 49.5 µM 7.78E-4 52 0.00169
20 SGTC_3286 9117135 2.90 37.2 µM 8.68E-4 62 0.00186

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.252 6.70E-50 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.195 4.74E-30 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.179 1.36E-25 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.169 7.29E-23 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.144 3.98E-17 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.143 7.35E-17 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.136 2.65E-15 YLL038C ENT4 hom Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
0.136 3.16E-15 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.134 8.04E-15 YIL022W TIM44 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
0.132 1.47E-14 YIR008C PRI1 het Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.132 1.51E-14 YIL101C XBP1 hom Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.123 7.58E-13 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.123 7.37E-13 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.123 8.31E-13 YLL026W HSP104 hom Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.115 2.72E-11 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188