HIPHOP chemogenomics database

YJL013C / MAD3

Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL013C / MAD3

Click on Significant Values for Screen Details

Top fitness defect scores for YJL013C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2651	1327-0036	5.69	5.0 µM	4.44E-10		82	6.53E-9
2	SGTC_1678	st019265	4.32	80.2 µM	1.58E-6	60S ribosome export	14	7.77E-6
3	SGTC_3083	9117465	4.00	49.5 µM	7.85E-6		23	3.12E-5
4	SGTC_514	4466-0038	3.99	2.6 nM	8.34E-6	cell wall	152	3.29E-5
5	SGTC_1450	4112-4260	3.94	37.6 µM	1.08E-5	calcium & mitochondrial duress	28	4.12E-5
6	SGTC_1757	st041620	3.71	31.8 µM	3.18E-5		36	1.05E-4
7	SGTC_2789	5281112	3.60	71.4 µM	5.11E-5		51	1.59E-4
8	SGTC_3298	9116858	3.51	71.4 µM	7.67E-5		20	2.26E-4
9	SGTC_2246	7293507	3.30	91.6 µM	1.82E-4		52	4.77E-4
10	SGTC_873	0591-4978	3.28	488.0 µM	1.97E-4		34	5.12E-4
11	SGTC_545	4218-0007	3.18	221.0 µM	2.98E-4		56	7.34E-4
12	SGTC_2784	7567673	3.16	72.7 µM	3.25E-4	iron homeostasis	65	7.92E-4
13	SGTC_2772	vinpocetine	3.13	57.1 µM	3.66E-4		56	8.78E-4
14	SGTC_779	1360-0071	3.10	233.0 µM	4.11E-4	fatty acid desaturase (OLE1)	71	9.71E-4
15	SGTC_1845	st056753	3.08	3.0 µM	4.38E-4	TSC3-RPN4	71	0.00103
16	SGTC_2369	9071472	3.07	200.0 µM	4.60E-4		49	0.00107
17	SGTC_1851	st056236	3.02	18.9 µM	5.55E-4		52	0.00126
18	SGTC_1464	k208-0027	2.96	110.0 µM	7.01E-4		60	0.00154
19	SGTC_3106	9121286	2.93	49.5 µM	7.78E-4		52	0.00169
20	SGTC_3286	9117135	2.90	37.2 µM	8.68E-4		62	0.00186

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.252	6.70E-50	YIL019W	FAF1	het	Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.195	4.74E-30	YJL019W	MPS3	het	Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.179	1.36E-25	YFL037W	TUB2	het	Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.169	7.29E-23	YOR297C	TIM18	hom	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.144	3.98E-17	YJL005W	CYR1	het	Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.143	7.35E-17	YMR036C	MIH1	hom	Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.136	2.65E-15	YLL038C	ENT4	hom	Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
0.136	3.16E-15	YMR048W	CSM3	hom	Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.134	8.04E-15	YIL022W	TIM44	het	Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
0.132	1.47E-14	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.132	1.51E-14	YIL101C	XBP1	hom	Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.123	7.58E-13	YER060W-A	FCY22	hom	Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.123	7.37E-13	YIL135C	VHS2	hom	Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.123	8.31E-13	YLL026W	HSP104	hom	Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.115	2.72E-11	YML103C	NUP188	hom	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188