YJL024C / APS3

Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL024C / APS3

Click on Significant Values for Screen Details ID:SGTC_91|Compound:0417-1751|FD-Score:4.18|P-value:4.44E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:4.15|P-value:5.03E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:5.90|P-value:1.53E-8 ID:SGTC_263|Compound:alizarin|FD-Score:4.38|P-value:1.98E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.03|P-value:7.19E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.93|P-value:1.04E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.64|P-value:2.96E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:4.11|P-value:5.74E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.23|P-value:3.60E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:8.83|P-value:5.61E-17 ID:SGTC_391|Compound:gliotoxin|FD-Score:4.57|P-value:8.91E-6 ID:SGTC_445|Compound:e6 berbamine|FD-Score:7.63|P-value:3.83E-13 ID:SGTC_518|Compound:1021-0119|FD-Score:3.31|P-value:9.50E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:4.13|P-value:5.39E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:14.70|P-value:1.71E-43 ID:SGTC_588|Compound:3970-1144|FD-Score:-3.37|P-value:7.31E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.37|P-value:7.81E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.69|P-value:2.51E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.75|P-value:2.22E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.13|P-value:7.45E-7 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.35|P-value:7.74E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.70|P-value:4.64E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.51|P-value:4.55E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:4.87|P-value:2.41E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:9.57|P-value:1.22E-19 ID:SGTC_922|Compound:1866-0035|FD-Score:4.42|P-value:1.69E-5 ID:SGTC_1080|Compound:nisoldipine|FD-Score:4.79|P-value:3.51E-6 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.01|P-value:1.29E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.98|P-value:1.45E-6 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:-3.55|P-value:4.01E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:4.55|P-value:9.81E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.92|P-value:1.19E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.31|P-value:9.62E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.44|P-value:6.21E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.94|P-value:1.76E-6 ID:SGTC_1581|Compound:himbacine|FD-Score:5.94|P-value:1.20E-8 ID:SGTC_1687|Compound:st020778|FD-Score:17.20|P-value:2.13E-59 ID:SGTC_1689|Compound:st020265|FD-Score:-3.52|P-value:4.48E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.42|P-value:6.83E-4 ID:SGTC_1743|Compound:st037606|FD-Score:4.96|P-value:1.63E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.74|P-value:4.26E-6 ID:SGTC_1773|Compound:st048542|FD-Score:5.38|P-value:2.23E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:8.31|P-value:2.88E-15 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.69|P-value:5.34E-6 ID:SGTC_1806|Compound:st050005|FD-Score:3.40|P-value:7.14E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.29|P-value:9.57E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:6.95|P-value:3.41E-11 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.87|P-value:2.46E-6 ID:SGTC_1936|Compound:st074407|FD-Score:3.92|P-value:1.17E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-5.08|P-value:8.22E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.32|P-value:9.31E-4 ID:SGTC_1986|Compound:st071812|FD-Score:4.07|P-value:6.64E-5 ID:SGTC_1992|Compound:st073643|FD-Score:7.08|P-value:1.50E-11 ID:SGTC_2026|Compound:5148700|FD-Score:3.78|P-value:1.94E-4 ID:SGTC_2173|Compound:5763948|FD-Score:3.69|P-value:2.70E-4 ID:SGTC_2184|Compound:6075246|FD-Score:3.70|P-value:2.58E-4 ID:SGTC_2218|Compound:6965428|FD-Score:-3.29|P-value:9.56E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.67|P-value:2.88E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-5.40|P-value:1.78E-7 ID:SGTC_2300|Compound:7976697|FD-Score:9.66|P-value:5.72E-20 ID:SGTC_2364|Compound:9055074|FD-Score:3.77|P-value:2.06E-4 ID:SGTC_2370|Compound:9071495|FD-Score:4.64|P-value:6.79E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.50|P-value:5.11E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.32|P-value:2.55E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.74|P-value:2.05E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.34|P-value:8.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.64|P-value:6.78E-6 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.12|P-value:4.45E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:11.20|P-value:2.36E-26 ID:SGTC_2781|Compound:5567276|FD-Score:-3.66|P-value:2.75E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.83|P-value:1.62E-4 ID:SGTC_2968|Compound:9090663|FD-Score:3.76|P-value:2.11E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-3.51|P-value:4.55E-4 ID:SGTC_2979|Compound:9012552|FD-Score:4.69|P-value:5.30E-6 ID:SGTC_2981|Compound:9013952|FD-Score:5.62|P-value:6.68E-8 ID:SGTC_3215|Compound:9129942|FD-Score:-3.68|P-value:2.54E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:4.18|P-value:4.44E-5 ID:SGTC_147|Compound:4466-0538|FD-Score:4.15|P-value:5.03E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:5.90|P-value:1.53E-8 ID:SGTC_263|Compound:alizarin|FD-Score:4.38|P-value:1.98E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.03|P-value:7.19E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.93|P-value:1.04E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-3.64|P-value:2.96E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:4.11|P-value:5.74E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:4.23|P-value:3.60E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:8.83|P-value:5.61E-17 ID:SGTC_391|Compound:gliotoxin|FD-Score:4.57|P-value:8.91E-6 ID:SGTC_445|Compound:e6 berbamine|FD-Score:7.63|P-value:3.83E-13 ID:SGTC_518|Compound:1021-0119|FD-Score:3.31|P-value:9.50E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:4.13|P-value:5.39E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:14.70|P-value:1.71E-43 ID:SGTC_588|Compound:3970-1144|FD-Score:-3.37|P-value:7.31E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.37|P-value:7.81E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.69|P-value:2.51E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.75|P-value:2.22E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.13|P-value:7.45E-7 ID:SGTC_716|Compound:1683-5115|FD-Score:-3.35|P-value:7.74E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.70|P-value:4.64E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:-3.51|P-value:4.55E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:4.87|P-value:2.41E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:9.57|P-value:1.22E-19 ID:SGTC_922|Compound:1866-0035|FD-Score:4.42|P-value:1.69E-5 ID:SGTC_1080|Compound:nisoldipine|FD-Score:4.79|P-value:3.51E-6 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:5.01|P-value:1.29E-6 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.98|P-value:1.45E-6 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:-3.55|P-value:4.01E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:4.55|P-value:9.81E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.92|P-value:1.19E-4 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.31|P-value:9.62E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.44|P-value:6.21E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.94|P-value:1.76E-6 ID:SGTC_1581|Compound:himbacine|FD-Score:5.94|P-value:1.20E-8 ID:SGTC_1687|Compound:st020778|FD-Score:17.20|P-value:2.13E-59 ID:SGTC_1689|Compound:st020265|FD-Score:-3.52|P-value:4.48E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.42|P-value:6.83E-4 ID:SGTC_1743|Compound:st037606|FD-Score:4.96|P-value:1.63E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.74|P-value:4.26E-6 ID:SGTC_1773|Compound:st048542|FD-Score:5.38|P-value:2.23E-7 ID:SGTC_1788|Compound:fluconazole|FD-Score:8.31|P-value:2.88E-15 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.69|P-value:5.34E-6 ID:SGTC_1806|Compound:st050005|FD-Score:3.40|P-value:7.14E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.29|P-value:9.57E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:6.95|P-value:3.41E-11 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.87|P-value:2.46E-6 ID:SGTC_1936|Compound:st074407|FD-Score:3.92|P-value:1.17E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-5.08|P-value:8.22E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.32|P-value:9.31E-4 ID:SGTC_1986|Compound:st071812|FD-Score:4.07|P-value:6.64E-5 ID:SGTC_1992|Compound:st073643|FD-Score:7.08|P-value:1.50E-11 ID:SGTC_2026|Compound:5148700|FD-Score:3.78|P-value:1.94E-4 ID:SGTC_2173|Compound:5763948|FD-Score:3.69|P-value:2.70E-4 ID:SGTC_2184|Compound:6075246|FD-Score:3.70|P-value:2.58E-4 ID:SGTC_2218|Compound:6965428|FD-Score:-3.29|P-value:9.56E-4 ID:SGTC_2238|Compound:6671620|FD-Score:3.67|P-value:2.88E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-5.40|P-value:1.78E-7 ID:SGTC_2300|Compound:7976697|FD-Score:9.66|P-value:5.72E-20 ID:SGTC_2364|Compound:9055074|FD-Score:3.77|P-value:2.06E-4 ID:SGTC_2370|Compound:9071495|FD-Score:4.64|P-value:6.79E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.50|P-value:5.11E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.32|P-value:2.55E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.74|P-value:2.05E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:3.34|P-value:8.68E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.64|P-value:6.78E-6 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.12|P-value:4.45E-9 ID:SGTC_2779|Compound:methotrexate|FD-Score:11.20|P-value:2.36E-26 ID:SGTC_2781|Compound:5567276|FD-Score:-3.66|P-value:2.75E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.83|P-value:1.62E-4 ID:SGTC_2968|Compound:9090663|FD-Score:3.76|P-value:2.11E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-3.51|P-value:4.55E-4 ID:SGTC_2979|Compound:9012552|FD-Score:4.69|P-value:5.30E-6 ID:SGTC_2981|Compound:9013952|FD-Score:5.62|P-value:6.68E-8 ID:SGTC_3215|Compound:9129942|FD-Score:-3.68|P-value:2.54E-4

Top fitness defect scores for YJL024C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1687 st020778 17.20 55.4 µM 2.13E-59 12 6.32E-67
2 SGTC_528 3492-0019 14.70 172.0 µM 1.71E-43 calcium & mitochondrial duress 16 5.96E-49
3 SGTC_2779 methotrexate 11.20 400.0 µM 2.36E-26 5 1.39E-29
4 SGTC_2300 7976697 9.66 71.6 µM 5.72E-20 ERG2 8 2.28E-22
5 SGTC_841 0251-0215 9.57 91.2 µM 1.22E-19 16 5.39E-22
6 SGTC_363 0455-0021 8.83 3.1 µM 5.61E-17 azole & statin 11 5.46E-19
7 SGTC_1788 fluconazole 8.31 20.0 µM 2.88E-15 azole & statin 17 4.67E-17
8 SGTC_445 e6 berbamine 7.63 66.0 µM 3.83E-13 NEO1-PIK1 15 1.17E-14
9 SGTC_1992 st073643 7.08 23.3 µM 1.50E-11 mitochondrial processes 1 7.37E-13
10 SGTC_1873 rapamycin 6.95 2.0 nM 3.41E-11 21 1.86E-12
11 SGTC_2737 bleomycin 6.12 30.5 nM 4.45E-9 Golgi 32 4.55E-10
12 SGTC_1581 himbacine 5.94 57.9 µM 1.20E-8 Golgi 19 1.39E-9
13 SGTC_245 methotrexate 5.90 289.6 µM 1.53E-8 13 1.83E-9
14 SGTC_2981 9013952 5.62 39.0 µM 6.68E-8 17 9.66E-9
15 SGTC_1773 st048542 5.38 73.6 µM 2.23E-7 23 3.77E-8
16 SGTC_682 0kpi-0099 5.13 17.0 µM 7.45E-7 34 1.47E-7
17 SGTC_1110 3-hydroxy-3'-methoxyflavone 5.01 1.2 µM 1.29E-6 iron homeostasis 25 2.73E-7
18 SGTC_1258 0671-0043 4.98 57.1 µM 1.45E-6 8 3.10E-7
19 SGTC_1743 st037606 4.96 46.4 µM 1.63E-6 9 3.55E-7
20 SGTC_1495 4469-0029 4.94 237.0 µM 1.76E-6 73 3.86E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.445 1.61E-162 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.439 8.26E-158 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
0.382 9.60E-117 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.361 7.92E-104 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.359 8.18E-103 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.352 1.31E-98 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.324 9.12E-83 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.305 4.54E-73 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.280 1.10E-61 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.272 6.15E-58 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.265 6.13E-55 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
0.265 7.97E-55 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.258 3.53E-52 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.252 9.78E-50 YGR164W_d YGR164W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.243 1.77E-46 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance